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The map label for this gene is glcD [H]

Identifier: 138895043

GI number: 138895043

Start: 1465255

End: 1466577

Strand: Direct

Name: glcD [H]

Synonym: GTNG_1381

Alternate gene names: 138895043

Gene position: 1465255-1466577 (Clockwise)

Preceding gene: 138895042

Following gene: 138895044

Centisome position: 41.27

GC content: 54.57

Gene sequence:

>1323_bases
ATGCTCTCGTTGACATGGCTGGATGAGTTATGTGCGTTTTTGTCTCCGGAACAAATGGAAGTAAACGAATCTCCTCATCC
GCTTGGCAATAGCGGCGCGGTGACCGTTTACCCGCAGACGGAAGAAGAAATCGCCGCTGTGCTCCGTTATGCCGACCATC
AGGGCAAAAAAGTTGTTGTCATGGGCCGCGGTACGAAACGAGGGTTTGGCGGACAGCGGGAACAGGCGGATATTTTGCTG
TCCTTAGAAAACTATGCAGGCATTGTCGAACATGCGGCTGCCGACATGATGGTCACTGTGAAAGCGGGGACGGTGTTTGG
CGATTTGCAGCGTGAGCTTGCCCCGTACCGACAAAAAGTAGCCTTAGATCCGTTCTGGCCGGATGAAGCGACGGTTGGTG
GGGTGATTGCGGCGAACGACAGCGGTCCGAAGCGGCTTGGCTACGGAGCGGCACGCGATTTGGTCATTGGGCTGCGCATT
GTGTATCCGAACGGTACGGTCATCCGTTCCGGCGGTAAAGTGGTGAAAAACGTCGCCGGTTATGACATGAACAAACTGTT
TATCGGGGCGATGGGGACGCTCGGTGTGCTGAGTGAGGTTACATTAAAGCTCCGGCCGCTTGCGAAATATGAAAGTGTTG
TATTGCTGTCGTTTCCAAGTGGCGATGTCCAATCGATTCACTCGTTCGCTGTTTCCTTCCTAGATACGATGATGGAGCCG
ATTTGTTTTGAACTGCTTACCCCGACGCTCGCCGAACGATTGACGGGACGACCTGTCTATACGCTCGTGATCGGATTTGA
AGATGTGGAAAAGGCCGTTCACTACCAGGAGCAGTGGGTGGAGCGCCTCTGTCCAAAAGCAGCTTCAATGGCCGTTTTAG
CCGGCGAGGAGGCAGAAGCGTTTTGGCGGACATTTTATACGATCGCGCCGAACGGGCGTCTGACGACATCGGGAGGGGGC
GTTGAAGCGGCGGTGAAAATTGGCGTTGTCAACTTGGATGTGCTCTCGGTTTTGCGAGAAAGCGAGTTAATCGCGGATCG
CTGCCACGTTCAAATTGAGGCGCACGGCGGATTGGGCCATGGGCTTTGCCAAGCGTATGTGCGCGGCAGCGAAGAGGGAG
TGATCGCCGCCGTTGAGGAGCTGCGCCAAATGGCGGTTCGCTTGGGCGGCTATGCGGTCGTCAGGCATTTGCCATTTTTC
TTGCGCCAACGCATCGATGTCTGGGGAGGAAAGCCAGCGTACTTTTTCCTGCTCGAAGGGATTAAGCGGAAAATCGATCC
GAATAAAACGGTCAACGATCAGCGCTTTATTGGGGGGATTTAA

Upstream 100 bases:

>100_bases
TGCAGGAGGGGGATTGGCTGAAACGTCAAGCACGTTTCGCTTTTTTTAAAAACTAAAAAAGAAACGTTGTTTCGTATTAC
GGAACAAAGGAGGGGCAATC

Downstream 100 bases:

>100_bases
CGATGAGTACAGTGACGAGTGAGCGGACGGGCAGCCCGGCGTGCGCAACCGCAAGCCTTGGCAACTACCGGTTTGCCGAC
TTGCCCGACCCGAGCAAATG

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLSLTWLDELCAFLSPEQMEVNESPHPLGNSGAVTVYPQTEEEIAAVLRYADHQGKKVVVMGRGTKRGFGGQREQADILL
SLENYAGIVEHAAADMMVTVKAGTVFGDLQRELAPYRQKVALDPFWPDEATVGGVIAANDSGPKRLGYGAARDLVIGLRI
VYPNGTVIRSGGKVVKNVAGYDMNKLFIGAMGTLGVLSEVTLKLRPLAKYESVVLLSFPSGDVQSIHSFAVSFLDTMMEP
ICFELLTPTLAERLTGRPVYTLVIGFEDVEKAVHYQEQWVERLCPKAASMAVLAGEEAEAFWRTFYTIAPNGRLTTSGGG
VEAAVKIGVVNLDVLSVLRESELIADRCHVQIEAHGGLGHGLCQAYVRGSEEGVIAAVEELRQMAVRLGGYAVVRHLPFF
LRQRIDVWGGKPAYFFLLEGIKRKIDPNKTVNDQRFIGGI

Sequences:

>Translated_440_residues
MLSLTWLDELCAFLSPEQMEVNESPHPLGNSGAVTVYPQTEEEIAAVLRYADHQGKKVVVMGRGTKRGFGGQREQADILL
SLENYAGIVEHAAADMMVTVKAGTVFGDLQRELAPYRQKVALDPFWPDEATVGGVIAANDSGPKRLGYGAARDLVIGLRI
VYPNGTVIRSGGKVVKNVAGYDMNKLFIGAMGTLGVLSEVTLKLRPLAKYESVVLLSFPSGDVQSIHSFAVSFLDTMMEP
ICFELLTPTLAERLTGRPVYTLVIGFEDVEKAVHYQEQWVERLCPKAASMAVLAGEEAEAFWRTFYTIAPNGRLTTSGGG
VEAAVKIGVVNLDVLSVLRESELIADRCHVQIEAHGGLGHGLCQAYVRGSEEGVIAAVEELRQMAVRLGGYAVVRHLPFF
LRQRIDVWGGKPAYFFLLEGIKRKIDPNKTVNDQRFIGGI
>Mature_440_residues
MLSLTWLDELCAFLSPEQMEVNESPHPLGNSGAVTVYPQTEEEIAAVLRYADHQGKKVVVMGRGTKRGFGGQREQADILL
SLENYAGIVEHAAADMMVTVKAGTVFGDLQRELAPYRQKVALDPFWPDEATVGGVIAANDSGPKRLGYGAARDLVIGLRI
VYPNGTVIRSGGKVVKNVAGYDMNKLFIGAMGTLGVLSEVTLKLRPLAKYESVVLLSFPSGDVQSIHSFAVSFLDTMMEP
ICFELLTPTLAERLTGRPVYTLVIGFEDVEKAVHYQEQWVERLCPKAASMAVLAGEEAEAFWRTFYTIAPNGRLTTSGGG
VEAAVKIGVVNLDVLSVLRESELIADRCHVQIEAHGGLGHGLCQAYVRGSEEGVIAAVEELRQMAVRLGGYAVVRHLPFF
LRQRIDVWGGKPAYFFLLEGIKRKIDPNKTVNDQRFIGGI

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=402, Percent_Identity=26.3681592039801, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI119964728, Length=260, Percent_Identity=26.5384615384615, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI37595754, Length=425, Percent_Identity=24.9411764705882, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI4501993, Length=191, Percent_Identity=25.130890052356, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI48994914, Length=150, Percent_Identity=35.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789351, Length=187, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=299, Percent_Identity=27.0903010033445, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71992381, Length=303, Percent_Identity=26.4026402640264, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71992392, Length=220, Percent_Identity=28.1818181818182, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556096, Length=195, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17534361, Length=431, Percent_Identity=23.4338747099768, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320027, Length=246, Percent_Identity=30.4878048780488, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320023, Length=427, Percent_Identity=23.6533957845433, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320764, Length=165, Percent_Identity=29.6969696969697, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24653753, Length=191, Percent_Identity=29.3193717277487, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 47995; Mature: 47995

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLTWLDELCAFLSPEQMEVNESPHPLGNSGAVTVYPQTEEEIAAVLRYADHQGKKVVV
CCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEE
MGRGTKRGFGGQREQADILLSLENYAGIVEHAAADMMVTVKAGTVFGDLQRELAPYRQKV
EECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHE
ALDPFWPDEATVGGVIAANDSGPKRLGYGAARDLVIGLRIVYPNGTVIRSGGKVVKNVAG
ECCCCCCCCCCCCCEEEECCCCCCCCCCCHHCCEEEEEEEECCCCCEEECCCHHHHHHCC
YDMNKLFIGAMGTLGVLSEVTLKLRPLAKYESVVLLSFPSGDVQSIHSFAVSFLDTMMEP
CCHHHHEEEHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
ICFELLTPTLAERLTGRPVYTLVIGFEDVEKAVHYQEQWVERLCPKAASMAVLAGEEAEA
HHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCHHHCEEEEECCHHHH
FWRTFYTIAPNGRLTTSGGGVEAAVKIGVVNLDVLSVLRESELIADRCHVQIEAHGGLGH
HHHEEEEECCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHHHHHEEEEEEEECCCCCH
GLCQAYVRGSEEGVIAAVEELRQMAVRLGGYAVVRHLPFFLRQRIDVWGGKPAYFFLLEG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IKRKIDPNKTVNDQRFIGGI
HHHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MLSLTWLDELCAFLSPEQMEVNESPHPLGNSGAVTVYPQTEEEIAAVLRYADHQGKKVVV
CCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEE
MGRGTKRGFGGQREQADILLSLENYAGIVEHAAADMMVTVKAGTVFGDLQRELAPYRQKV
EECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHE
ALDPFWPDEATVGGVIAANDSGPKRLGYGAARDLVIGLRIVYPNGTVIRSGGKVVKNVAG
ECCCCCCCCCCCCCEEEECCCCCCCCCCCHHCCEEEEEEEECCCCCEEECCCHHHHHHCC
YDMNKLFIGAMGTLGVLSEVTLKLRPLAKYESVVLLSFPSGDVQSIHSFAVSFLDTMMEP
CCHHHHEEEHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
ICFELLTPTLAERLTGRPVYTLVIGFEDVEKAVHYQEQWVERLCPKAASMAVLAGEEAEA
HHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCHHHCEEEEECCHHHH
FWRTFYTIAPNGRLTTSGGGVEAAVKIGVVNLDVLSVLRESELIADRCHVQIEAHGGLGH
HHHEEEEECCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHHHHHEEEEEEEECCCCCH
GLCQAYVRGSEEGVIAAVEELRQMAVRLGGYAVVRHLPFFLRQRIDVWGGKPAYFFLLEG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IKRKIDPNKTVNDQRFIGGI
HHHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]