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The map label for this gene is glcF [H]

Identifier: 138895044

GI number: 138895044

Start: 1466580

End: 1467938

Strand: Direct

Name: glcF [H]

Synonym: GTNG_1382

Alternate gene names: 138895044

Gene position: 1466580-1467938 (Clockwise)

Preceding gene: 138895043

Following gene: 138895045

Centisome position: 41.31

GC content: 55.11

Gene sequence:

>1359_bases
ATGAGTACAGTGACGAGTGAGCGGACGGGCAGCCCGGCGTGCGCAACCGCAAGCCTTGGCAACTACCGGTTTGCCGACTT
GCCCGACCCGAGCAAATGGGCGGATTGCGTTCATTGCGGTATGTGTTTAGAGTCGTGTCCGACGTACGAACAAACCGGTG
AAGAACAACATTCTCCACGTGGTCGTGTTCACTTGATGAAGTCGGTCGGTGAAGGGAAGCTTGATTTAACGCCGGATTTG
CTTGACCCGATTTTTACGTGCCTAGACTGCCGCGCCTGTACGACAGCGTGTCCGGCTGATGTTGATGTCGGCGGTCTCAT
TGAACAAGTGCGCGGCCAGCTCCGCCAGGCGGTGCCGCTCACGGGGTGGAAGGCGCTTGTGAACGACTTCTTCTTAAAAG
GGATATTCCCGCATCCGTCGCGCCTTGATTGGCTTGGTCGTCTGCTGAAGCTGTACCAACAAAGTGGACTGCAGACAGTG
GCGCGCAAAACGGGGATGCTGAGGGTAATGCCGGACCATTTGGCCGAGATGGAAGCGATTTTGCCGAAGGTCGGCACGCC
GGTGCGGAAAAAGTATAGGAACGAGCCGTTGATTAAGGCAAAAGGGGAAACAAAGCGGACGGTCGCGATCTTGACTGGCT
GCATCATGGATGTGATGTTCTCAGATATTAACGAAGCGACGATCCGCGTGTTGACCCGTAACGGCAACGATGTCGTCATC
GTCTCTGATCAGACGTGTTGCGGGGCGCTTCACGTTCATGCCGGCGACCGCGAAACGGGGCGGAAGCTCGCCAAGCAAAA
CATCGAGGCGTTTCAAGGTGTGGACCATGTGATCGTCAACGCCGCTGGCTGCGGTGCGATGTTGAAAGAATATCCGGAAT
TGTTCCGCCATGATCCAGAATGGCATGAAAAGGCGGAACAGTTTGCCGCGAAAGTCGAGGATGTGTCTAAATTTTTGTAT
GATACGGGCTTCAAGGCGCCAAAAGCAGAATTGAATGTGCGCCTCACGTATCACGATGCGTGCCATTTAGCTCATGGACA
AGGCATCCGTAAGGAGCCGCGCGCCGTCATTGAAAGCATTCCAGGCGTGGAGATGGTCGCCATGCCGAACGCTGACCGCT
GCTGCGGCAGTGCAGGGATCTACAACCTCACCCATCCGGACATGGCGCAAGCAGTGCTTGAAAGCAAAATGCAAAACGTG
CCACAAGATGTGGAGCTCATCTCGATGGGCAACCCGGGCTGCATGTTGCAAATGGCGCTGGGCGTCATGAAGCATGGGCG
AAATCAAAAAATCGTCCATACGGTGCAATTGCTCGATTGGGCATACCAAAAAGAGGAAGGGAAGGACGTGCGCATATGA

Upstream 100 bases:

>100_bases
GAGGAAAGCCAGCGTACTTTTTCCTGCTCGAAGGGATTAAGCGGAAAATCGATCCGAATAAAACGGTCAACGATCAGCGC
TTTATTGGGGGGATTTAACG

Downstream 100 bases:

>100_bases
GAAAACGGCCCATTGCAACGAATGACCCGCATATTCGGGCGCTTGCCCGCATCGTTGGGGAAGAGGCGATTTTGTATCGG
AAAGAAGACTTAATCGCCTA

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSTVTSERTGSPACATASLGNYRFADLPDPSKWADCVHCGMCLESCPTYEQTGEEQHSPRGRVHLMKSVGEGKLDLTPDL
LDPIFTCLDCRACTTACPADVDVGGLIEQVRGQLRQAVPLTGWKALVNDFFLKGIFPHPSRLDWLGRLLKLYQQSGLQTV
ARKTGMLRVMPDHLAEMEAILPKVGTPVRKKYRNEPLIKAKGETKRTVAILTGCIMDVMFSDINEATIRVLTRNGNDVVI
VSDQTCCGALHVHAGDRETGRKLAKQNIEAFQGVDHVIVNAAGCGAMLKEYPELFRHDPEWHEKAEQFAAKVEDVSKFLY
DTGFKAPKAELNVRLTYHDACHLAHGQGIRKEPRAVIESIPGVEMVAMPNADRCCGSAGIYNLTHPDMAQAVLESKMQNV
PQDVELISMGNPGCMLQMALGVMKHGRNQKIVHTVQLLDWAYQKEEGKDVRI

Sequences:

>Translated_452_residues
MSTVTSERTGSPACATASLGNYRFADLPDPSKWADCVHCGMCLESCPTYEQTGEEQHSPRGRVHLMKSVGEGKLDLTPDL
LDPIFTCLDCRACTTACPADVDVGGLIEQVRGQLRQAVPLTGWKALVNDFFLKGIFPHPSRLDWLGRLLKLYQQSGLQTV
ARKTGMLRVMPDHLAEMEAILPKVGTPVRKKYRNEPLIKAKGETKRTVAILTGCIMDVMFSDINEATIRVLTRNGNDVVI
VSDQTCCGALHVHAGDRETGRKLAKQNIEAFQGVDHVIVNAAGCGAMLKEYPELFRHDPEWHEKAEQFAAKVEDVSKFLY
DTGFKAPKAELNVRLTYHDACHLAHGQGIRKEPRAVIESIPGVEMVAMPNADRCCGSAGIYNLTHPDMAQAVLESKMQNV
PQDVELISMGNPGCMLQMALGVMKHGRNQKIVHTVQLLDWAYQKEEGKDVRI
>Mature_451_residues
STVTSERTGSPACATASLGNYRFADLPDPSKWADCVHCGMCLESCPTYEQTGEEQHSPRGRVHLMKSVGEGKLDLTPDLL
DPIFTCLDCRACTTACPADVDVGGLIEQVRGQLRQAVPLTGWKALVNDFFLKGIFPHPSRLDWLGRLLKLYQQSGLQTVA
RKTGMLRVMPDHLAEMEAILPKVGTPVRKKYRNEPLIKAKGETKRTVAILTGCIMDVMFSDINEATIRVLTRNGNDVVIV
SDQTCCGALHVHAGDRETGRKLAKQNIEAFQGVDHVIVNAAGCGAMLKEYPELFRHDPEWHEKAEQFAAKVEDVSKFLYD
TGFKAPKAELNVRLTYHDACHLAHGQGIRKEPRAVIESIPGVEMVAMPNADRCCGSAGIYNLTHPDMAQAVLESKMQNVP
QDVELISMGNPGCMLQMALGVMKHGRNQKIVHTVQLLDWAYQKEEGKDVRI

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=420, Percent_Identity=30.7142857142857, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786497, Length=235, Percent_Identity=25.1063829787234, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788576, Length=370, Percent_Identity=24.8648648648649, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49915; Mature: 49784

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVTSERTGSPACATASLGNYRFADLPDPSKWADCVHCGMCLESCPTYEQTGEEQHSPR
CCCCCCCCCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCHHCCCC
GRVHLMKSVGEGKLDLTPDLLDPIFTCLDCRACTTACPADVDVGGLIEQVRGQLRQAVPL
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
TGWKALVNDFFLKGIFPHPSRLDWLGRLLKLYQQSGLQTVARKTGMLRVMPDHLAEMEAI
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHH
LPKVGTPVRKKYRNEPLIKAKGETKRTVAILTGCIMDVMFSDINEATIRVLTRNGNDVVI
HHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEE
VSDQTCCGALHVHAGDRETGRKLAKQNIEAFQGVDHVIVNAAGCGAMLKEYPELFRHDPE
EECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCH
WHEKAEQFAAKVEDVSKFLYDTGFKAPKAELNVRLTYHDACHLAHGQGIRKEPRAVIESI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECHHHHHCCCCCCCHHHHHHHHC
PGVEMVAMPNADRCCGSAGIYNLTHPDMAQAVLESKMQNVPQDVELISMGNPGCMLQMAL
CCCEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
GVMKHGRNQKIVHTVQLLDWAYQKEEGKDVRI
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STVTSERTGSPACATASLGNYRFADLPDPSKWADCVHCGMCLESCPTYEQTGEEQHSPR
CCCCCCCCCCCCEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCHHCCCC
GRVHLMKSVGEGKLDLTPDLLDPIFTCLDCRACTTACPADVDVGGLIEQVRGQLRQAVPL
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
TGWKALVNDFFLKGIFPHPSRLDWLGRLLKLYQQSGLQTVARKTGMLRVMPDHLAEMEAI
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHH
LPKVGTPVRKKYRNEPLIKAKGETKRTVAILTGCIMDVMFSDINEATIRVLTRNGNDVVI
HHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEE
VSDQTCCGALHVHAGDRETGRKLAKQNIEAFQGVDHVIVNAAGCGAMLKEYPELFRHDPE
EECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCH
WHEKAEQFAAKVEDVSKFLYDTGFKAPKAELNVRLTYHDACHLAHGQGIRKEPRAVIESI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECHHHHHCCCCCCCHHHHHHHHC
PGVEMVAMPNADRCCGSAGIYNLTHPDMAQAVLESKMQNVPQDVELISMGNPGCMLQMAL
CCCEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
GVMKHGRNQKIVHTVQLLDWAYQKEEGKDVRI
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]