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The map label for this gene is dat [H]

Identifier: 138894253

GI number: 138894253

Start: 642734

End: 643597

Strand: Reverse

Name: dat [H]

Synonym: GTNG_0579

Alternate gene names: 138894253

Gene position: 643597-642734 (Counterclockwise)

Preceding gene: 138894256

Following gene: 138894239

Centisome position: 18.13

GC content: 49.65

Gene sequence:

>864_bases
ATGTCAGTGAAACCGTATGTATTGACCGAACGTGGCGTTCTCCGCTGCGAGCAAGTAACATATCCCATGGAAGAACGCGG
CTTGCAGTTTGGCGATGGCGTCTATGAGGTAGTGAGCCTATACAACGGAGCATATATTTGGCTTCAAGAACATCTCGACC
GCCTGTACCGTTCGGCAGCGGCTATTCGCTTGTCTGTCCCATTTGCTCCCGAAGAGCTGTCCGAGCGGCTTGAACAGCTT
CGCCGTATGAATGATGTGCACGAAGATGCGATTTTGTATTTGCAAGTGACAAGAGGCAGCTTTCCGCGCAACCATGCGTT
CCCGACAGAAAATCGTCCCAATTTGTATGCCTACATCCAACCGATGGCAAGAAAAACAGATGAAATGACACACGGCGTAC
GCGCCATTTTAACGAAAGACGTTCGTTGGGAGTACTGCTATATTAAAAGCTTGAATTTGTTGCCGAACGTGCTAGCGAAA
CAAGAAGCGGTCGAGCGTGGAGCGTTTGAAGCCATTCTCCACCGTGACGGCGTCGTGACCGAAGGCAGTTCGTCCAATAT
GTTTCTTGTCAAAGGCGAAACGGTGTACACCCATCCGGCGACAGAACGGATTTTAAACGGCATCGTCCGCACGAAAGTGA
AGCAGTTTTGCGCCGATCTTGGCATCCCACTGATTGAAAAAGCGTTTTCGGTTCATGATATTGCCGAAGCAGACGAACTA
TTTTTAACAAGCACAACCTCAGCTATTATTCCGATCATTCAAGTCGATGACACAACAATCGGCAACGGCCAGCCAGGAAC
GGTAACAAAATCGTTGCAAGCCGCTTACCAAAAGGCGACACAACCGGCCACAAGCAACGCTTGA

Upstream 100 bases:

>100_bases
AAACCCCCGCTTAGAGCAAAGCGGGAGTAGGGGAGGAAATCAATTTATGATATAATCGAATTGATTTATTATTCGCGCAA
TTGAAAAGGAGTGCACTTTT

Downstream 100 bases:

>100_bases
CTGGCTTAGTTGAACAAACGAGGTGTCCCCACGCTCTTCCTTGGGACACCTCGTTTTGTTTATGAGCTGAGCACAGCGCG
GTCGCTGGCGAATTGGCTGC

Product: D-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSVKPYVLTERGVLRCEQVTYPMEERGLQFGDGVYEVVSLYNGAYIWLQEHLDRLYRSAAAIRLSVPFAPEELSERLEQL
RRMNDVHEDAILYLQVTRGSFPRNHAFPTENRPNLYAYIQPMARKTDEMTHGVRAILTKDVRWEYCYIKSLNLLPNVLAK
QEAVERGAFEAILHRDGVVTEGSSSNMFLVKGETVYTHPATERILNGIVRTKVKQFCADLGIPLIEKAFSVHDIAEADEL
FLTSTTSAIIPIIQVDDTTIGNGQPGTVTKSLQAAYQKATQPATSNA

Sequences:

>Translated_287_residues
MSVKPYVLTERGVLRCEQVTYPMEERGLQFGDGVYEVVSLYNGAYIWLQEHLDRLYRSAAAIRLSVPFAPEELSERLEQL
RRMNDVHEDAILYLQVTRGSFPRNHAFPTENRPNLYAYIQPMARKTDEMTHGVRAILTKDVRWEYCYIKSLNLLPNVLAK
QEAVERGAFEAILHRDGVVTEGSSSNMFLVKGETVYTHPATERILNGIVRTKVKQFCADLGIPLIEKAFSVHDIAEADEL
FLTSTTSAIIPIIQVDDTTIGNGQPGTVTKSLQAAYQKATQPATSNA
>Mature_286_residues
SVKPYVLTERGVLRCEQVTYPMEERGLQFGDGVYEVVSLYNGAYIWLQEHLDRLYRSAAAIRLSVPFAPEELSERLEQLR
RMNDVHEDAILYLQVTRGSFPRNHAFPTENRPNLYAYIQPMARKTDEMTHGVRAILTKDVRWEYCYIKSLNLLPNVLAKQ
EAVERGAFEAILHRDGVVTEGSSSNMFLVKGETVYTHPATERILNGIVRTKVKQFCADLGIPLIEKAFSVHDIAEADELF
LTSTTSAIIPIIQVDDTTIGNGQPGTVTKSLQAAYQKATQPATSNA

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=26.551724137931, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787338, Length=257, Percent_Identity=26.0700389105058, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32280; Mature: 32149

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKPYVLTERGVLRCEQVTYPMEERGLQFGDGVYEVVSLYNGAYIWLQEHLDRLYRSAA
CCCCCEEECCCCCHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHH
AIRLSVPFAPEELSERLEQLRRMNDVHEDAILYLQVTRGSFPRNHAFPTENRPNLYAYIQ
EEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEC
PMARKTDEMTHGVRAILTKDVRWEYCYIKSLNLLPNVLAKQEAVERGAFEAILHRDGVVT
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
EGSSSNMFLVKGETVYTHPATERILNGIVRTKVKQFCADLGIPLIEKAFSVHDIAEADEL
CCCCCCEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
FLTSTTSAIIPIIQVDDTTIGNGQPGTVTKSLQAAYQKATQPATSNA
EEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVKPYVLTERGVLRCEQVTYPMEERGLQFGDGVYEVVSLYNGAYIWLQEHLDRLYRSAA
CCCCEEECCCCCHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHH
AIRLSVPFAPEELSERLEQLRRMNDVHEDAILYLQVTRGSFPRNHAFPTENRPNLYAYIQ
EEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEC
PMARKTDEMTHGVRAILTKDVRWEYCYIKSLNLLPNVLAKQEAVERGAFEAILHRDGVVT
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
EGSSSNMFLVKGETVYTHPATERILNGIVRTKVKQFCADLGIPLIEKAFSVHDIAEADEL
CCCCCCEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
FLTSTTSAIIPIIQVDDTTIGNGQPGTVTKSLQAAYQKATQPATSNA
EEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]