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The map label for this gene is lacZ [H]

Identifier: 138894081

GI number: 138894081

Start: 460136

End: 461896

Strand: Direct

Name: lacZ [H]

Synonym: GTNG_0407

Alternate gene names: 138894081

Gene position: 460136-461896 (Clockwise)

Preceding gene: 138894080

Following gene: 138894083

Centisome position: 12.96

GC content: 35.04

Gene sequence:

>1761_bases
ATGAATAATGTCAAAGTCTATAACCGCACTGAATATCCTCGTCCGCAATTTAAACGCAAGGACTGGTTAAATTTAAATGG
AAAATGGAGTTTTGCATTTGATGATGAAAATATAGGAGAACGTAACGAGTGGTATAAAAATTTATCAACTTCAATGGAAA
TACAAGTTCCCTTTACTTATGAAACAAAAGCGAGCGGGATTGGAGACGAAACGTTTCACCCTGTTGTGTGGTACCAACGC
TCTTTTAAAATTCCAGAAGATCAAATAGGCAAAAAGGTGATCTTAAGGTTTCAAGCATCTGATTATTTTACAAAGGTTTG
GGTAAATGGACATTTCGTAGGTGACCATAAAGGGGGGAACTCAGCGTTTGCTTTTGATATTACGAATGTGATTGACCAAT
ATGGCGAGAATGAGCTAGTCGTAAAAGTAGAAGATAGTTTAAGTTGCTATCAGCCGAGAGGAAAACAACGGTGGATGGAT
AAGAATTTTGGCTGTTGGTATGTGCAAACTACTGGTATATGGCAGACTGTTTGGCTGGAATTTTTAAACCATCAAACCAT
AAAGTCTGTCAAAATGACGCCAAATATCGATAATCACTCGATACGATTTGATTATGATCTCGATGTGTCATTACATGAGG
ACTTGCGACTAGAAACTGTGATTAATTTTAAAGGAAGAGAAATAAAAAGATTTTCTATCAATCCTAATCGCCCTCAATTT
CATTTAGAAGTGGATCTTTTATCGGAGATCTCTGAATGGAAAGTATTTCTATGGTCGCCTGAGGATCCGAATCTTTATGA
TGTTGTTTTTCGTTTGTATGACAATGAGCAATTAGTTGATGAGGTTGAATCCTATTTTGGAATGCGAAAAATCTCTATAA
AAGATGGAAAAGTATTATTAAATAATAAACCACTTTATCAAAAACTTTTATTAGATCAGGGATATTGGCCTGACACGTTG
TTAACCCCCCCTTCTGATGAAGCAATAATTGATGATATTAATAAAACAATACAAATGGGGTTTAATGGTGTACGTAAACA
CCAAAAAATAGAAGATGAAAGATTTTTGTATTGGTGTGATAAAAAAGGATTGTTAGTGTGGTCTGAAATGGCTGCGACTT
ATGAATTTTCGGATCAGGCTGTAGAAAACTTTACAAAGGAATGGCTTGAAATTGTCCAGCAGTATTATAATCATCCCTCT
ATTATCACTTGGGTCCCCTTTAATGAATCATGGGGTATACCAAATGTAGCATATGATAAAAAACAGCAGTCGTTTACTGA
ATCAATCTATTATCTCACTAAATCGATCGATCAAATGAGACCGGTTATTGTCAATGATGGTTGGGAACATACAGTTTCTG
ATATTATTACTCTGCATGATTATGAAGAATGTGGAGAAAAATTTTATGAACGATATAAAGATAAAGATCTTATTTTAAAT
AATCAGATCCCCCATAACAAAGATAAGTATGCGTTTGCTGAAGGTTACACTTATAAAGGGCAACCGGTTATCATCTCTGA
ATATGGTGGGATTGCATTTAAGGACGAAAAAGGCTGGGGATATGGACACCAAGTGGAGACTGAAGACGAATTTTTAAGAA
GGTATCAAAGTATCACAGACGCGATAAAAAAATTACCGTATATATCCGGATATTGCTACACCCAAATTACAGATGTGCAA
CAAGAAGTTAATGGCTTATTAAAAGAAGACCGAACTCCTAAGATCGCACTAGAAAAAATAAAAAAAGTTAATGATTCCTA
A

Upstream 100 bases:

>100_bases
ATTCCAGTTATTATCGCATTTCTAATCTTTGAAAAACAAATTGTAAAAGGAATTAGTTTCACAGGTATTAAAGGATAAAC
ATAGAAAGGAAGAATGGCAG

Downstream 100 bases:

>100_bases
GAAAAGAATAGATAAGGATCATTAAGTAGGGCTGTCTATATTGACTTGCGTCATACTTAATCCCATAGTCCATAATAAAA
GCTAAGCAACACGTTGCTTA

Product: putative hydrolase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MNNVKVYNRTEYPRPQFKRKDWLNLNGKWSFAFDDENIGERNEWYKNLSTSMEIQVPFTYETKASGIGDETFHPVVWYQR
SFKIPEDQIGKKVILRFQASDYFTKVWVNGHFVGDHKGGNSAFAFDITNVIDQYGENELVVKVEDSLSCYQPRGKQRWMD
KNFGCWYVQTTGIWQTVWLEFLNHQTIKSVKMTPNIDNHSIRFDYDLDVSLHEDLRLETVINFKGREIKRFSINPNRPQF
HLEVDLLSEISEWKVFLWSPEDPNLYDVVFRLYDNEQLVDEVESYFGMRKISIKDGKVLLNNKPLYQKLLLDQGYWPDTL
LTPPSDEAIIDDINKTIQMGFNGVRKHQKIEDERFLYWCDKKGLLVWSEMAATYEFSDQAVENFTKEWLEIVQQYYNHPS
IITWVPFNESWGIPNVAYDKKQQSFTESIYYLTKSIDQMRPVIVNDGWEHTVSDIITLHDYEECGEKFYERYKDKDLILN
NQIPHNKDKYAFAEGYTYKGQPVIISEYGGIAFKDEKGWGYGHQVETEDEFLRRYQSITDAIKKLPYISGYCYTQITDVQ
QEVNGLLKEDRTPKIALEKIKKVNDS

Sequences:

>Translated_586_residues
MNNVKVYNRTEYPRPQFKRKDWLNLNGKWSFAFDDENIGERNEWYKNLSTSMEIQVPFTYETKASGIGDETFHPVVWYQR
SFKIPEDQIGKKVILRFQASDYFTKVWVNGHFVGDHKGGNSAFAFDITNVIDQYGENELVVKVEDSLSCYQPRGKQRWMD
KNFGCWYVQTTGIWQTVWLEFLNHQTIKSVKMTPNIDNHSIRFDYDLDVSLHEDLRLETVINFKGREIKRFSINPNRPQF
HLEVDLLSEISEWKVFLWSPEDPNLYDVVFRLYDNEQLVDEVESYFGMRKISIKDGKVLLNNKPLYQKLLLDQGYWPDTL
LTPPSDEAIIDDINKTIQMGFNGVRKHQKIEDERFLYWCDKKGLLVWSEMAATYEFSDQAVENFTKEWLEIVQQYYNHPS
IITWVPFNESWGIPNVAYDKKQQSFTESIYYLTKSIDQMRPVIVNDGWEHTVSDIITLHDYEECGEKFYERYKDKDLILN
NQIPHNKDKYAFAEGYTYKGQPVIISEYGGIAFKDEKGWGYGHQVETEDEFLRRYQSITDAIKKLPYISGYCYTQITDVQ
QEVNGLLKEDRTPKIALEKIKKVNDS
>Mature_586_residues
MNNVKVYNRTEYPRPQFKRKDWLNLNGKWSFAFDDENIGERNEWYKNLSTSMEIQVPFTYETKASGIGDETFHPVVWYQR
SFKIPEDQIGKKVILRFQASDYFTKVWVNGHFVGDHKGGNSAFAFDITNVIDQYGENELVVKVEDSLSCYQPRGKQRWMD
KNFGCWYVQTTGIWQTVWLEFLNHQTIKSVKMTPNIDNHSIRFDYDLDVSLHEDLRLETVINFKGREIKRFSINPNRPQF
HLEVDLLSEISEWKVFLWSPEDPNLYDVVFRLYDNEQLVDEVESYFGMRKISIKDGKVLLNNKPLYQKLLLDQGYWPDTL
LTPPSDEAIIDDINKTIQMGFNGVRKHQKIEDERFLYWCDKKGLLVWSEMAATYEFSDQAVENFTKEWLEIVQQYYNHPS
IITWVPFNESWGIPNVAYDKKQQSFTESIYYLTKSIDQMRPVIVNDGWEHTVSDIITLHDYEECGEKFYERYKDKDLILN
NQIPHNKDKYAFAEGYTYKGQPVIISEYGGIAFKDEKGWGYGHQVETEDEFLRRYQSITDAIKKLPYISGYCYTQITDVQ
QEVNGLLKEDRTPKIALEKIKKVNDS

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=538, Percent_Identity=22.6765799256506, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1787903, Length=632, Percent_Identity=22.4683544303797, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI48994920, Length=622, Percent_Identity=22.6688102893891, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786539, Length=642, Percent_Identity=23.5202492211838, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17510825, Length=608, Percent_Identity=23.6842105263158, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24655438, Length=618, Percent_Identity=23.462783171521, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922582, Length=618, Percent_Identity=23.462783171521, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI62471735, Length=618, Percent_Identity=23.462783171521, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 69313; Mature: 69313

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVKVYNRTEYPRPQFKRKDWLNLNGKWSFAFDDENIGERNEWYKNLSTSMEIQVPFTY
CCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEEEEEEE
ETKASGIGDETFHPVVWYQRSFKIPEDQIGKKVILRFQASDYFTKVWVNGHFVGDHKGGN
CCCCCCCCCCCCCCEEEEECCCCCCHHHCCCEEEEEEECCCCEEEEEEEEEEEECCCCCC
SAFAFDITNVIDQYGENELVVKVEDSLSCYQPRGKQRWMDKNFGCWYVQTTGIWQTVWLE
CEEEEEHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHH
FLNHQTIKSVKMTPNIDNHSIRFDYDLDVSLHEDLRLETVINFKGREIKRFSINPNRPQF
HHCCHHHHEEEECCCCCCCEEEEEEEECCEECCCCEEEEEEECCCCEEEEEECCCCCCEE
HLEVDLLSEISEWKVFLWSPEDPNLYDVVFRLYDNEQLVDEVESYFGMRKISIKDGKVLL
EEEHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCEEEEEECCCEEEE
NNKPLYQKLLLDQGYWPDTLLTPPSDEAIIDDINKTIQMGFNGVRKHQKIEDERFLYWCD
CCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEC
KKGLLVWSEMAATYEFSDQAVENFTKEWLEIVQQYYNHPSIITWVPFNESWGIPNVAYDK
CCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
KQQSFTESIYYLTKSIDQMRPVIVNDGWEHTVSDIITLHDYEECGEKFYERYKDKDLILN
HHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHEECCHHHHHHHHHHHHCCCCEEEC
NQIPHNKDKYAFAEGYTYKGQPVIISEYGGIAFKDEKGWGYGHQVETEDEFLRRYQSITD
CCCCCCCCCEEEECCCEECCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
AIKKLPYISGYCYTQITDVQQEVNGLLKEDRTPKIALEKIKKVNDS
HHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNNVKVYNRTEYPRPQFKRKDWLNLNGKWSFAFDDENIGERNEWYKNLSTSMEIQVPFTY
CCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEEEEEEE
ETKASGIGDETFHPVVWYQRSFKIPEDQIGKKVILRFQASDYFTKVWVNGHFVGDHKGGN
CCCCCCCCCCCCCCEEEEECCCCCCHHHCCCEEEEEEECCCCEEEEEEEEEEEECCCCCC
SAFAFDITNVIDQYGENELVVKVEDSLSCYQPRGKQRWMDKNFGCWYVQTTGIWQTVWLE
CEEEEEHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHH
FLNHQTIKSVKMTPNIDNHSIRFDYDLDVSLHEDLRLETVINFKGREIKRFSINPNRPQF
HHCCHHHHEEEECCCCCCCEEEEEEEECCEECCCCEEEEEEECCCCEEEEEECCCCCCEE
HLEVDLLSEISEWKVFLWSPEDPNLYDVVFRLYDNEQLVDEVESYFGMRKISIKDGKVLL
EEEHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCEEEEEECCCEEEE
NNKPLYQKLLLDQGYWPDTLLTPPSDEAIIDDINKTIQMGFNGVRKHQKIEDERFLYWCD
CCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEC
KKGLLVWSEMAATYEFSDQAVENFTKEWLEIVQQYYNHPSIITWVPFNESWGIPNVAYDK
CCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
KQQSFTESIYYLTKSIDQMRPVIVNDGWEHTVSDIITLHDYEECGEKFYERYKDKDLILN
HHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHEECCHHHHHHHHHHHHCCCCEEEC
NQIPHNKDKYAFAEGYTYKGQPVIISEYGGIAFKDEKGWGYGHQVETEDEFLRRYQSITD
CCCCCCCCCEEEECCCEECCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
AIKKLPYISGYCYTQITDVQQEVNGLLKEDRTPKIALEKIKKVNDS
HHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA