Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 138894077

GI number: 138894077

Start: 454348

End: 455754

Strand: Direct

Name: emrB [H]

Synonym: GTNG_0403

Alternate gene names: 138894077

Gene position: 454348-455754 (Clockwise)

Preceding gene: 138894075

Following gene: 138894078

Centisome position: 12.8

GC content: 48.05

Gene sequence:

>1407_bases
ATGGGAAAAGAAAAATTACCAAAAGGAATGATTAGCATCGCGTGGATTTTGGTGCTTGGTGCAATTCTGCCTATGTTGGA
CTCCACGATCATCAATATTGCGGTGAACGATTTAGCCAAAGCGTTCTCAACCACCTTTGCGGTTACGCAATGGGTGGTGA
CTGGATATGTTCTCGCTTTGGGGATGGCAGTCCCGCTTTCCGGTTGGCTGATGCAAAAATATGATGGCAAGAAAGTATAC
ATGGTTGCATTGGGACTGTTCTTAATCAGCTCGCTTCTTTGCGGTCTTGCATGGGATATGCAGAGCTTAATCGCATTTCG
TATGCTGCAGGGGCTCGCTTCCGGCATCATAATCCCAGCGCTGACGACTTTGGTTGTACAAGCTGCTGGCAGTGAAAACC
TGGGCCGTGTCATGTCCGTTGTCGGTATTCCGGTGGTTTTTGGCCCTATTGCCGGACCGGTTATCGGAGGATTCATTTTA
CAGCACTTATCGTGGCATTGGTTGTTTTTTGTAAACCTTCCTATAGGGGGCATAGCATTACTCATCGCACAATGGAAATT
GCCGAAGTTCGAGGCAATGAATAAATCTGCAAAACTGGACTGGATAGGGATTCTGTTGCTGGCCATGTTATCTGGAATGG
TTGTCTTTGGCGTGACGGAGATTAGGGCACAGAACACGCGAGCAACTGGCATCCTGGCATTTGGCATTGGCGCTATCTCG
TTGGTGGCATACTTGCTTTATGCGTCGAAGAGAAAGGATCAAGCATTGATACCACTGGATCTGTTCAAATCAAAAAATTT
CAGTGCTGCGTTTCTTTCACTGTTTTTAGCCGGATTTGCCACCAATGGGCCTATGCTTCTCTTCCCGATGTTTTTTCAAA
ATGTACTTGAGCTCAACGTCATTACAGCGGCACTTTGGCTGATTCCCCAAGGTGTGGGAATGCTGGTCACACGGCCTTTG
ATCGGTAAAATGACGGATAAGCTCGGGGCACGGTATGTAGTACTGCCTTCCATTGCCATAACCATCATCGGGACACTTCC
ATTTGTATTCTTTGATGCCGACACGTCGCAATGGCTCATATGGTTGGTACTGTTTGTACGAGGAATAGGCGTCGGAGGGA
TCACGGTCCCTGTGATGAGTGATTCTTACGTAGGGCTCCAAAAGCTGCAAGTCCCAGCAGCCAGCGTTGCCACCCGCATC
ATCCAAAACGTTGGAGCCGCCTTTGGTTCCGCCATTTTGGCAACGGTGGTCAGCAACGCTCTAAATGTGAAGGAGGCTAC
AGTAGCCAACTTTGCCGGCGCTTATCACGCAGGTTTTATCACAAGCTTAATCTTCATGGTAATTAGCATTCTGCCTGCAC
TGTTTTTGACAAACAAGATGAGCATAGCGAGGGTCGAAGGACAGTAA

Upstream 100 bases:

>100_bases
TTTAAAAAAATTTTTAAGGTACATATTGAACCTTATTTTTAAATCGTTTATACTAATTAAGGTACAACATGTACCTTAAT
TAAAAATAGGAGATGGAAAT

Downstream 100 bases:

>100_bases
TCTGCATCTAAGGCTTTGATTGTGAAATATATGAGTTGCTACTCTCATTAGAAATCAGAATGGCCTCTTGTCGGTGATCA
TTGACAAGAGGCCATTTACT

Product: transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGKEKLPKGMISIAWILVLGAILPMLDSTIINIAVNDLAKAFSTTFAVTQWVVTGYVLALGMAVPLSGWLMQKYDGKKVY
MVALGLFLISSLLCGLAWDMQSLIAFRMLQGLASGIIIPALTTLVVQAAGSENLGRVMSVVGIPVVFGPIAGPVIGGFIL
QHLSWHWLFFVNLPIGGIALLIAQWKLPKFEAMNKSAKLDWIGILLLAMLSGMVVFGVTEIRAQNTRATGILAFGIGAIS
LVAYLLYASKRKDQALIPLDLFKSKNFSAAFLSLFLAGFATNGPMLLFPMFFQNVLELNVITAALWLIPQGVGMLVTRPL
IGKMTDKLGARYVVLPSIAITIIGTLPFVFFDADTSQWLIWLVLFVRGIGVGGITVPVMSDSYVGLQKLQVPAASVATRI
IQNVGAAFGSAILATVVSNALNVKEATVANFAGAYHAGFITSLIFMVISILPALFLTNKMSIARVEGQ

Sequences:

>Translated_468_residues
MGKEKLPKGMISIAWILVLGAILPMLDSTIINIAVNDLAKAFSTTFAVTQWVVTGYVLALGMAVPLSGWLMQKYDGKKVY
MVALGLFLISSLLCGLAWDMQSLIAFRMLQGLASGIIIPALTTLVVQAAGSENLGRVMSVVGIPVVFGPIAGPVIGGFIL
QHLSWHWLFFVNLPIGGIALLIAQWKLPKFEAMNKSAKLDWIGILLLAMLSGMVVFGVTEIRAQNTRATGILAFGIGAIS
LVAYLLYASKRKDQALIPLDLFKSKNFSAAFLSLFLAGFATNGPMLLFPMFFQNVLELNVITAALWLIPQGVGMLVTRPL
IGKMTDKLGARYVVLPSIAITIIGTLPFVFFDADTSQWLIWLVLFVRGIGVGGITVPVMSDSYVGLQKLQVPAASVATRI
IQNVGAAFGSAILATVVSNALNVKEATVANFAGAYHAGFITSLIFMVISILPALFLTNKMSIARVEGQ
>Mature_467_residues
GKEKLPKGMISIAWILVLGAILPMLDSTIINIAVNDLAKAFSTTFAVTQWVVTGYVLALGMAVPLSGWLMQKYDGKKVYM
VALGLFLISSLLCGLAWDMQSLIAFRMLQGLASGIIIPALTTLVVQAAGSENLGRVMSVVGIPVVFGPIAGPVIGGFILQ
HLSWHWLFFVNLPIGGIALLIAQWKLPKFEAMNKSAKLDWIGILLLAMLSGMVVFGVTEIRAQNTRATGILAFGIGAISL
VAYLLYASKRKDQALIPLDLFKSKNFSAAFLSLFLAGFATNGPMLLFPMFFQNVLELNVITAALWLIPQGVGMLVTRPLI
GKMTDKLGARYVVLPSIAITIIGTLPFVFFDADTSQWLIWLVLFVRGIGVGGITVPVMSDSYVGLQKLQVPAASVATRII
QNVGAAFGSAILATVVSNALNVKEATVANFAGAYHAGFITSLIFMVISILPALFLTNKMSIARVEGQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=455, Percent_Identity=26.1538461538462, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789042, Length=433, Percent_Identity=25.4041570438799, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=26.3157894736842, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788392, Length=329, Percent_Identity=28.2674772036474, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87081983, Length=314, Percent_Identity=27.3885350318471, Blast_Score=108, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319770, Length=393, Percent_Identity=24.9363867684478, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=36.0902255639098, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=170, Percent_Identity=28.2352941176471, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=180, Percent_Identity=30.5555555555556, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50125; Mature: 49994

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
GKEKLPKGMISIAWILVLGAILPMLDSTIINIAVNDLAKAFSTTFAVTQWVVTGYVLAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]