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The map label for this gene is yfjO [H]

Identifier: 138894075

GI number: 138894075

Start: 451773

End: 453167

Strand: Direct

Name: yfjO [H]

Synonym: GTNG_0401

Alternate gene names: 138894075

Gene position: 451773-453167 (Clockwise)

Preceding gene: 138894074

Following gene: 138894077

Centisome position: 12.72

GC content: 52.83

Gene sequence:

>1395_bases
ATGACAAAGCAACAAACGAAGGTAAAGATTCAAAAAGGCGATCAATTTCCGCTGACGATTCAGCGCATCGGCATTAACGG
TGAAGGGGTCGGTTATTTTCAAAAACAAGTTGTCTTTGTCCCCGGCGCCTTGCCAGGTGAGGAAGTGATCGTTGAGGCGA
CAGACATTCACCCTACCTATGCTGAGGCGAAAATCAAACGCATCCGAAAGCGCTCGTCTCACCGCGTCACGCCGTCATGC
CCGCTCTATAACCAGTGCGGCGGCTGCCAGCTGCAGCACTTGTCGTACGAGGCGCAGCTTGAAGCGAAGCGTGACATTGT
CATCCAAGCGTTGCGTCGTCACGTCCGTCGTCTCGATGTCGACAAAATTGACATCCGCCCGACGATCGGGATGAACGATC
CGTGGCATTACCGAAACAAAAGCCAGTTTCAAGTCGGAGTGAAAAAAGGAAAGGTGCTCGCCGGGCTGTACGGCCTGAAC
TCACACCGGCTCATCGACTTGTCGGAATGTCGCGTCCAACACCCACAAACGACGCGCGTGACCAACATTGTCAAAACGAT
TTTGCAAGATTTGCGCATCCCCATTTACAATGAGCGGACAAGAACAGGCGTCGTACGGACGATCGTCGCCCGCGTTGGCT
TTCATACGGGGGACATCCAGATCGTTCTTGTCACGGCGACGAAAGATATTCCGCGCAAAGAGCTGCTCATTGACGAAATT
CGTCGCCGTCTTCCAGAAGTGAAATCGATCGTGCAAAACATAAACGGCGAAAAAACCTCGCTCATTTTCGGCGACGAGAC
AGAAGTGCTAGCAGGAGATGAATACATTCAAGAAACGCTCGGCGATTTGTCATTTGAGCTCTCCGCGCGCGCCTTTTTCC
AGCTTAATCCCATCCAAACGGTGAAATTGTACGATGAAGTGAAAAAGGCATCCGCTCTCACCGGAACAGAGAGGATCGTT
GATGCCTATTGCGGCGTCGGCACGATCGGTCTTTGGCTCGCTCGCGACGCCAAAGAAGTGCGCGGCATGGATACGATTCC
GGAAGCAATCGAAGATGCAAAGAAAAACGCCAAAAAACACGGCTTTGAGAACACGCATTATGTGGTCGGAAAAGCGGAAT
ATTGGCTGCCAAAATGGGTGAAACAAGGATGGAAGCCGGACGTCGTCATCGTCGACCCGCCCCGCGTCGGCTGCGACCGA
GCGCTGCTTGAGACGATCCTTCGCGTTCGCCCGCAAACGGTCGTCTACGTCTCATGCAACCCATCCAGCCTTGCCCGTGA
TCTTGATACCTTGGCTAGGGCGTATCGCGTCGACTACATTCAACCGGTCGACATGTTCCCGCATACGGCGCATGTGGAGA
CGGTTGCGAAGTTGATTTTAACAAGATATTCATAA

Upstream 100 bases:

>100_bases
CGACTGCTAGCGAGCTGGCATTGCTCGGCGAACGATGGAAGCCGTACACGAGTTATGCGGCTCTTTATTTGTGGAGAAGT
ATTGAATGAGAGGGACTGAT

Downstream 100 bases:

>100_bases
ATGGAAACAAGGATAATGGTTGTTCATCAAAATCTGTATGAAAAAGGTCCCTTTTATGGTACCTGTGTCACTGCTTGATC
TTCCTTATTTAGGGATTTTT

Product: RNA methyltransferase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTKQQTKVKIQKGDQFPLTIQRIGINGEGVGYFQKQVVFVPGALPGEEVIVEATDIHPTYAEAKIKRIRKRSSHRVTPSC
PLYNQCGGCQLQHLSYEAQLEAKRDIVIQALRRHVRRLDVDKIDIRPTIGMNDPWHYRNKSQFQVGVKKGKVLAGLYGLN
SHRLIDLSECRVQHPQTTRVTNIVKTILQDLRIPIYNERTRTGVVRTIVARVGFHTGDIQIVLVTATKDIPRKELLIDEI
RRRLPEVKSIVQNINGEKTSLIFGDETEVLAGDEYIQETLGDLSFELSARAFFQLNPIQTVKLYDEVKKASALTGTERIV
DAYCGVGTIGLWLARDAKEVRGMDTIPEAIEDAKKNAKKHGFENTHYVVGKAEYWLPKWVKQGWKPDVVIVDPPRVGCDR
ALLETILRVRPQTVVYVSCNPSSLARDLDTLARAYRVDYIQPVDMFPHTAHVETVAKLILTRYS

Sequences:

>Translated_464_residues
MTKQQTKVKIQKGDQFPLTIQRIGINGEGVGYFQKQVVFVPGALPGEEVIVEATDIHPTYAEAKIKRIRKRSSHRVTPSC
PLYNQCGGCQLQHLSYEAQLEAKRDIVIQALRRHVRRLDVDKIDIRPTIGMNDPWHYRNKSQFQVGVKKGKVLAGLYGLN
SHRLIDLSECRVQHPQTTRVTNIVKTILQDLRIPIYNERTRTGVVRTIVARVGFHTGDIQIVLVTATKDIPRKELLIDEI
RRRLPEVKSIVQNINGEKTSLIFGDETEVLAGDEYIQETLGDLSFELSARAFFQLNPIQTVKLYDEVKKASALTGTERIV
DAYCGVGTIGLWLARDAKEVRGMDTIPEAIEDAKKNAKKHGFENTHYVVGKAEYWLPKWVKQGWKPDVVIVDPPRVGCDR
ALLETILRVRPQTVVYVSCNPSSLARDLDTLARAYRVDYIQPVDMFPHTAHVETVAKLILTRYS
>Mature_463_residues
TKQQTKVKIQKGDQFPLTIQRIGINGEGVGYFQKQVVFVPGALPGEEVIVEATDIHPTYAEAKIKRIRKRSSHRVTPSCP
LYNQCGGCQLQHLSYEAQLEAKRDIVIQALRRHVRRLDVDKIDIRPTIGMNDPWHYRNKSQFQVGVKKGKVLAGLYGLNS
HRLIDLSECRVQHPQTTRVTNIVKTILQDLRIPIYNERTRTGVVRTIVARVGFHTGDIQIVLVTATKDIPRKELLIDEIR
RRLPEVKSIVQNINGEKTSLIFGDETEVLAGDEYIQETLGDLSFELSARAFFQLNPIQTVKLYDEVKKASALTGTERIVD
AYCGVGTIGLWLARDAKEVRGMDTIPEAIEDAKKNAKKHGFENTHYVVGKAEYWLPKWVKQGWKPDVVIVDPPRVGCDRA
LLETILRVRPQTVVYVSCNPSSLARDLDTLARAYRVDYIQPVDMFPHTAHVETVAKLILTRYS

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=355, Percent_Identity=28.7323943661972, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI51173878, Length=355, Percent_Identity=28.7323943661972, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI269784680, Length=232, Percent_Identity=31.8965517241379, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI269784676, Length=232, Percent_Identity=31.8965517241379, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI20149688, Length=232, Percent_Identity=31.8965517241379, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI269784678, Length=232, Percent_Identity=31.8965517241379, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1789148, Length=463, Percent_Identity=27.8617710583153, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787083, Length=387, Percent_Identity=26.0981912144703, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790403, Length=207, Percent_Identity=25.6038647342995, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=168, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322909, Length=435, Percent_Identity=25.7471264367816, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24666579, Length=415, Percent_Identity=27.710843373494, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52590; Mature: 52459

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQQTKVKIQKGDQFPLTIQRIGINGEGVGYFQKQVVFVPGALPGEEVIVEATDIHPTY
CCCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCHH
AEAKIKRIRKRSSHRVTPSCPLYNQCGGCQLQHLSYEAQLEAKRDIVIQALRRHVRRLDV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
DKIDIRPTIGMNDPWHYRNKSQFQVGVKKGKVLAGLYGLNSHRLIDLSECRVQHPQTTRV
CEEECCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEHHHHHCCCCCHHHH
TNIVKTILQDLRIPIYNERTRTGVVRTIVARVGFHTGDIQIVLVTATKDIPRKELLIDEI
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
RRRLPEVKSIVQNINGEKTSLIFGDETEVLAGDEYIQETLGDLSFELSARAFFQLNPIQT
HHHHHHHHHHHHHCCCCEEEEEECCCCCEECCHHHHHHHHHCCEEEECCEEEEEECCCHH
VKLYDEVKKASALTGTERIVDAYCGVGTIGLWLARDAKEVRGMDTIPEAIEDAKKNAKKH
HHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHC
GFENTHYVVGKAEYWLPKWVKQGWKPDVVIVDPPRVGCDRALLETILRVRPQTVVYVSCN
CCCCCEEEEECCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECC
PSSLARDLDTLARAYRVDYIQPVDMFPHTAHVETVAKLILTRYS
HHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKQQTKVKIQKGDQFPLTIQRIGINGEGVGYFQKQVVFVPGALPGEEVIVEATDIHPTY
CCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCHH
AEAKIKRIRKRSSHRVTPSCPLYNQCGGCQLQHLSYEAQLEAKRDIVIQALRRHVRRLDV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
DKIDIRPTIGMNDPWHYRNKSQFQVGVKKGKVLAGLYGLNSHRLIDLSECRVQHPQTTRV
CEEECCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEHHHHHCCCCCHHHH
TNIVKTILQDLRIPIYNERTRTGVVRTIVARVGFHTGDIQIVLVTATKDIPRKELLIDEI
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
RRRLPEVKSIVQNINGEKTSLIFGDETEVLAGDEYIQETLGDLSFELSARAFFQLNPIQT
HHHHHHHHHHHHHCCCCEEEEEECCCCCEECCHHHHHHHHHCCEEEECCEEEEEECCCHH
VKLYDEVKKASALTGTERIVDAYCGVGTIGLWLARDAKEVRGMDTIPEAIEDAKKNAKKH
HHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHC
GFENTHYVVGKAEYWLPKWVKQGWKPDVVIVDPPRVGCDRALLETILRVRPQTVVYVSCN
CCCCCEEEEECCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECC
PSSLARDLDTLARAYRVDYIQPVDMFPHTAHVETVAKLILTRYS
HHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]