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The map label for this gene is thiC

Identifier: 138894020

GI number: 138894020

Start: 393917

End: 395608

Strand: Direct

Name: thiC

Synonym: GTNG_0346

Alternate gene names: 138894020

Gene position: 393917-395608 (Clockwise)

Preceding gene: 138894019

Following gene: 138894021

Centisome position: 11.1

GC content: 52.78

Gene sequence:

>1692_bases
ATGGAAAACATTCGCTCGTTTATGTCATTTCCAGCCAGCCGGAAAGTGTACGTCGAAGGATCACGGCCGGACGTGCGCGT
GCCGATGAGAGAAATCGCGTTATCACCGACGAAGACACCGCAAGGAGAGGTGGAAAACAAACCGGTGCGCGTCTATGACA
CAACCGGTCCATACACAGATCCTGATTTTGAGCCGAATGTAGAAAAAGGATTGCCGCTTCTTCGCCGCAACTGGATTGTT
GAGCGAGGCGATGTCGAAGAAACGGAGCCACAGTCCACCGCCGGCCGTGCGGCGTCGCTTCCGTTTGCACGACGTCCGCT
GCGGGCGAAGCCGGGGAAAGTGGTCACGCAAATGCATTACGCCAAAAAAGGAATCATTACACCGGAAATGGAATTTGTCG
CGATTCGCGAACAAATCGACCCGGAAATCGTCCGCCAGGAGGTTGCCGCTGGCCGAGCGATCATCCCTTCGAATATTAAT
CATCCAGAAAGCGAACCGATGATCATCGGCCGCCGTTTTCATGTAAAAATTAACGCCAACATCGGCAACTCGGCTGTTTC
TTCCTCGATTGAGAATGAAGTGGAAAAACTGCTGTGGGCGGTACGCTGGGGTGCTGATACGGTGATGGATTTGTCGACCG
GCAAACAAATTCACGAAACGCGTGAATACATTATCCGCAATTCGCCAGTTCCAATCGGAACAGTGCCGATTTATCAGGCG
CTTGAGAAAGTAGGCGGCGTACCAGAGAAGTTGACATGGGACGTGTACCGCGAGACATTGATTGAGCAGGCTGAACAAGG
GGTTGACTACATGACGATCCACGCTGGTGTGCGGCTTCATTACATTCCGCTCACCGTCAATCGGACGACCGGCATCGTCT
CACGTGGCGGCTCAATCATCGCGCAATGGTGTTTGGCCCACCATGAAGAAAACTTCTTATATACGCATTTTGAAGAAATA
TGCGAGATTTTAAAACAATACGATGTTGCCATTTCGCTTGGTGACGGCTTGCGCCCGGGGTCCATTGCTGATGCAAACGA
CGAGGCACAGTTTGCTGAACTTGAGACGCTCGGTGAGCTGACGAAAATCGCTTGGGAGCATGACGTGCAAGTCATGATTG
AAGGACCGGGGCATATTCCGATGCAGAAAATCCGCGAAAACGTCGAGCGAGAACAAGAGCTTTGTCATGGTGCACCGTTT
TATACGTTAGGGCCGCTTGTCACCGACATTGCGCCGGGATACGACCACATCACGTCGGCGATTGGCGCCGCGATCATCGG
TGCCTACGGGACGTCCATGCTTTGCTATGTGACGCCGAAAGAACATTTAGGGTTACCAAATAAAGAGGATGTGCGCGTCG
GGGTCGTTACATATAAAATCGCCGCCCACGCCGCCGATTTGGCGAAAGGTCACCCAGCCGCCCAGCAGCGCGACGATGCG
CTCTCGAAAGCGCGCTTTGAGTTTCGCTGGAATGACCAGTTCAACTTATCGCTCGATCCGGAGCGGGCTCGCGAGTATCA
TGATGAAACATTGCCGGCTGAGGCGGCGAAAACCGCTCATTTTTGCTCGATGTGCGGGCCGAAATTTTGCTCGATGAACA
TCTCACACGAGCTGCAACGGAAAATCAAAGAAGAAGGAATGAAAGAAAAGGCGAACGAATTTGTGCGCGGCGGTTCATCG
CTGTATCGGTAA

Upstream 100 bases:

>100_bases
GTTTTTATTTTTCACGGCAAAGAGCGGCTCGTTTTTGCCTTTGCTTCGGCTGGCTTGGCCTCGGATCCTAAACCATCGTT
GATTTAGAGGGGGAATCCGT

Downstream 100 bases:

>100_bases
GGAGAGGATACGTTTTGTATACCGGACGTTAACTAGGCGTCCGGTTTTTCTTTTATGCTTATAAAGCGGTAGTGTAGATG
GGGGTGAGAAAAGCAACGAA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MENIRSFMSFPASRKVYVEGSRPDVRVPMREIALSPTKTPQGEVENKPVRVYDTTGPYTDPDFEPNVEKGLPLLRRNWIV
ERGDVEETEPQSTAGRAASLPFARRPLRAKPGKVVTQMHYAKKGIITPEMEFVAIREQIDPEIVRQEVAAGRAIIPSNIN
HPESEPMIIGRRFHVKINANIGNSAVSSSIENEVEKLLWAVRWGADTVMDLSTGKQIHETREYIIRNSPVPIGTVPIYQA
LEKVGGVPEKLTWDVYRETLIEQAEQGVDYMTIHAGVRLHYIPLTVNRTTGIVSRGGSIIAQWCLAHHEENFLYTHFEEI
CEILKQYDVAISLGDGLRPGSIADANDEAQFAELETLGELTKIAWEHDVQVMIEGPGHIPMQKIRENVEREQELCHGAPF
YTLGPLVTDIAPGYDHITSAIGAAIIGAYGTSMLCYVTPKEHLGLPNKEDVRVGVVTYKIAAHAADLAKGHPAAQQRDDA
LSKARFEFRWNDQFNLSLDPERAREYHDETLPAEAAKTAHFCSMCGPKFCSMNISHELQRKIKEEGMKEKANEFVRGGSS
LYR

Sequences:

>Translated_563_residues
MENIRSFMSFPASRKVYVEGSRPDVRVPMREIALSPTKTPQGEVENKPVRVYDTTGPYTDPDFEPNVEKGLPLLRRNWIV
ERGDVEETEPQSTAGRAASLPFARRPLRAKPGKVVTQMHYAKKGIITPEMEFVAIREQIDPEIVRQEVAAGRAIIPSNIN
HPESEPMIIGRRFHVKINANIGNSAVSSSIENEVEKLLWAVRWGADTVMDLSTGKQIHETREYIIRNSPVPIGTVPIYQA
LEKVGGVPEKLTWDVYRETLIEQAEQGVDYMTIHAGVRLHYIPLTVNRTTGIVSRGGSIIAQWCLAHHEENFLYTHFEEI
CEILKQYDVAISLGDGLRPGSIADANDEAQFAELETLGELTKIAWEHDVQVMIEGPGHIPMQKIRENVEREQELCHGAPF
YTLGPLVTDIAPGYDHITSAIGAAIIGAYGTSMLCYVTPKEHLGLPNKEDVRVGVVTYKIAAHAADLAKGHPAAQQRDDA
LSKARFEFRWNDQFNLSLDPERAREYHDETLPAEAAKTAHFCSMCGPKFCSMNISHELQRKIKEEGMKEKANEFVRGGSS
LYR
>Mature_563_residues
MENIRSFMSFPASRKVYVEGSRPDVRVPMREIALSPTKTPQGEVENKPVRVYDTTGPYTDPDFEPNVEKGLPLLRRNWIV
ERGDVEETEPQSTAGRAASLPFARRPLRAKPGKVVTQMHYAKKGIITPEMEFVAIREQIDPEIVRQEVAAGRAIIPSNIN
HPESEPMIIGRRFHVKINANIGNSAVSSSIENEVEKLLWAVRWGADTVMDLSTGKQIHETREYIIRNSPVPIGTVPIYQA
LEKVGGVPEKLTWDVYRETLIEQAEQGVDYMTIHAGVRLHYIPLTVNRTTGIVSRGGSIIAQWCLAHHEENFLYTHFEEI
CEILKQYDVAISLGDGLRPGSIADANDEAQFAELETLGELTKIAWEHDVQVMIEGPGHIPMQKIRENVEREQELCHGAPF
YTLGPLVTDIAPGYDHITSAIGAAIIGAYGTSMLCYVTPKEHLGLPNKEDVRVGVVTYKIAAHAADLAKGHPAAQQRDDA
LSKARFEFRWNDQFNLSLDPERAREYHDETLPAEAAKTAHFCSMCGPKFCSMNISHELQRKIKEEGMKEKANEFVRGGSS
LYR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=601, Percent_Identity=65.8901830282862, Blast_Score=799, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_GEOTN (A4IK74)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001124473.1
- STRING:   A4IK74
- GeneID:   4965290
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_0346
- NMPDR:   fig|420246.5.peg.332
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   A4IK74
- ProtClustDB:   PRK09284
- BioCyc:   GTHE420246:GTNG_0346-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 63051; Mature: 63051

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: BINDING 180-180 BINDING 209-209 BINDING 238-238 BINDING 274-274 BINDING 374-374 BINDING 401-401

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIRSFMSFPASRKVYVEGSRPDVRVPMREIALSPTKTPQGEVENKPVRVYDTTGPYTD
CCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCC
PDFEPNVEKGLPLLRRNWIVERGDVEETEPQSTAGRAASLPFARRPLRAKPGKVVTQMHY
CCCCCCHHHCCCHHHHCCEEECCCCCCCCCCHHCCCCCCCCHHCCCCCCCCCHHHHHHHH
AKKGIITPEMEFVAIREQIDPEIVRQEVAAGRAIIPSNINHPESEPMIIGRRFHVKINAN
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEEEEEEECC
IGNSAVSSSIENEVEKLLWAVRWGADTVMDLSTGKQIHETREYIIRNSPVPIGTVPIYQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
LEKVGGVPEKLTWDVYRETLIEQAEQGVDYMTIHAGVRLHYIPLTVNRTTGIVSRGGSII
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEECCCCHHHCCHHHH
AQWCLAHHEENFLYTHFEEICEILKQYDVAISLGDGLRPGSIADANDEAQFAELETLGEL
HHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
TKIAWEHDVQVMIEGPGHIPMQKIRENVEREQELCHGAPFYTLGPLVTDIAPGYDHITSA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHH
IGAAIIGAYGTSMLCYVTPKEHLGLPNKEDVRVGVVTYKIAAHAADLAKGHPAAQQRDDA
HHHHHHHHHCCCEEEEECCHHHCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHH
LSKARFEFRWNDQFNLSLDPERAREYHDETLPAEAAKTAHFCSMCGPKFCSMNISHELQR
HHHCEEEEEECCEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHH
KIKEEGMKEKANEFVRGGSSLYR
HHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MENIRSFMSFPASRKVYVEGSRPDVRVPMREIALSPTKTPQGEVENKPVRVYDTTGPYTD
CCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCC
PDFEPNVEKGLPLLRRNWIVERGDVEETEPQSTAGRAASLPFARRPLRAKPGKVVTQMHY
CCCCCCHHHCCCHHHHCCEEECCCCCCCCCCHHCCCCCCCCHHCCCCCCCCCHHHHHHHH
AKKGIITPEMEFVAIREQIDPEIVRQEVAAGRAIIPSNINHPESEPMIIGRRFHVKINAN
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEEEEEEECC
IGNSAVSSSIENEVEKLLWAVRWGADTVMDLSTGKQIHETREYIIRNSPVPIGTVPIYQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
LEKVGGVPEKLTWDVYRETLIEQAEQGVDYMTIHAGVRLHYIPLTVNRTTGIVSRGGSII
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEECCCCHHHCCHHHH
AQWCLAHHEENFLYTHFEEICEILKQYDVAISLGDGLRPGSIADANDEAQFAELETLGEL
HHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
TKIAWEHDVQVMIEGPGHIPMQKIRENVEREQELCHGAPFYTLGPLVTDIAPGYDHITSA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHH
IGAAIIGAYGTSMLCYVTPKEHLGLPNKEDVRVGVVTYKIAAHAADLAKGHPAAQQRDDA
HHHHHHHHHCCCEEEEECCHHHCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHH
LSKARFEFRWNDQFNLSLDPERAREYHDETLPAEAAKTAHFCSMCGPKFCSMNISHELQR
HHHCEEEEEECCEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHH
KIKEEGMKEKANEFVRGGSSLYR
HHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA