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The map label for this gene is gatB [H]

Identifier: 138893939

GI number: 138893939

Start: 295230

End: 296660

Strand: Direct

Name: gatB [H]

Synonym: GTNG_0263

Alternate gene names: 138893939

Gene position: 295230-296660 (Clockwise)

Preceding gene: 138893938

Following gene: 138893940

Centisome position: 8.32

GC content: 50.73

Gene sequence:

>1431_bases
ATGAATTTTGAAACGGTCATCGGACTTGAGGTGCACGTCGAGCTGAAAACAAAATCGAAAATTTTCTCAAGCAGCCCGAA
CGCATTCGGTGCGCCCCCGAATACGCAAACGAACGTCATCGATTTAGGGTATCCGGGTGTGCTGCCGGTCTTGAACCGGC
AAGCGGTCGAGTTTGCGATGAAGGCGGCGATGGCGTTAAACTGCGAAATCGCGACCGAGACAAAATTTGACCGCAAAAAC
TATTTTTATCCGGACAACCCGAAGGCGTACCAAATTTCGCAATACGACCAACCGCTTGGCAAAAACGGTTGGATTGAAAT
TGAAGTGAACGGGAAAAAGAAAAAAATCGGCATCACCCGCATCCATCTCGAGGAAGATGCCGGTAAATTGATGCATACAG
GCGACGGCTATTCGCTTGTTGACTTTAACCGTCAAGGGACGCCACTTATTGAGATCGTCTCCGAGCCGGACATCCGCTCG
CCGGAAGAAGCGTACGCCTACTTGGAAAAATTAAAAGCGATCATCCAATATACCGGCGTTTCCGACTGCAAAATGGAAGA
AGGGTCGCTTCGCTGCGACGCCAATATTTCGCTTCGCCCGCTCGGTTCGGACAAGTTCGGTACAAAAACGGAATTGAAAA
ACCTCAACTCATTCAACTTCGTCCGCATGGGGCTCGAGTACGAGGCGAAACGGCAAGAGAAAATTTTGCTCTCCGGTGGC
GTCATCCGTCAAGAAACGCGGCGGTTTGACGAAGCGACGAAAACGACCGTTTTGATGCGCGTCAAAGAAGGTTCGGAAGA
CTACCGTTACTTCCCAGAGCCGGATTTGGTCATGCTTTACATTGACGACGAATGGAAAGAGCGCGTCCGCGCCTCGATTC
CGGAGCTGCCGGACGCCCGCCGGAAGCGGTATGTCGAAGAGTGGGGCTTGCCAGAATATGACGCCAAAGTGCTCACACTG
ACAAAAGAAATGGCCGACTTCTTTGAAGCGACCGTTGCGAACGGCGCTGATCCGAAACTGGCGTCGAACTGGCTCATGGT
AGAAGTATCCGGTTACTTAAACTCCGAACAAAAAGAGCTTCATGACATCGCCTTGACGCCGGAAAGTCTAGCTGGGATGA
TCAAGCTCATCCAAAACGGCACGATTTCGTCGAAAATCGCGAAAAAAGTGTTTAAAGAGCTCGTTGAACACGGCGGCGAT
CCAGAGAAAATCGTCAAAGAAAAAGGGCTTGTGCAAATTTCCGACGAAGGAGCGCTGCGCAAAATCGTGCTAGAAGTGCT
AGATGCCAACCCGCAGTCGGTGGAAGACTATAAAAACGGTAAAGACCGTGCGCTCGGCTTCCTCGTTGGCCAAGTGATGA
AAGCAACGAAAGGGCAAGCGAACCCGCCGCTTGTCAACAAGCTGCTTGTTGAAGAAATCAATAAGCGATAA

Upstream 100 bases:

>100_bases
AAGCATTTTGATGAAAGCACGGTCTACCGTGTCGCTCACGCCTTTGAACAAGCGACCGATTACCATAAGCAAAAACCGGT
GTTATAAGGGGGGATAGACG

Downstream 100 bases:

>100_bases
ATCAAAAGACAAGGCTGCTGACCGGCAGCCTTGTCTTTTTATTTTATTTTTTAGACTTTTAAAAAAATACAATTGACATC
CCTCAATGGAAATGATAGAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETVIGLEVHVELKTKSKIFSSSPNAFGAPPNTQTNVIDLGYPGVLPVLNRQAVEFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQYDQPLGKNGWIEIEVNGKKKKIGITRIHLEEDAGKLMHTGDGYSLVDFNRQGTPLIEIVSEPDIRS
PEEAYAYLEKLKAIIQYTGVSDCKMEEGSLRCDANISLRPLGSDKFGTKTELKNLNSFNFVRMGLEYEAKRQEKILLSGG
VIRQETRRFDEATKTTVLMRVKEGSEDYRYFPEPDLVMLYIDDEWKERVRASIPELPDARRKRYVEEWGLPEYDAKVLTL
TKEMADFFEATVANGADPKLASNWLMVEVSGYLNSEQKELHDIALTPESLAGMIKLIQNGTISSKIAKKVFKELVEHGGD
PEKIVKEKGLVQISDEGALRKIVLEVLDANPQSVEDYKNGKDRALGFLVGQVMKATKGQANPPLVNKLLVEEINKR

Sequences:

>Translated_476_residues
MNFETVIGLEVHVELKTKSKIFSSSPNAFGAPPNTQTNVIDLGYPGVLPVLNRQAVEFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQYDQPLGKNGWIEIEVNGKKKKIGITRIHLEEDAGKLMHTGDGYSLVDFNRQGTPLIEIVSEPDIRS
PEEAYAYLEKLKAIIQYTGVSDCKMEEGSLRCDANISLRPLGSDKFGTKTELKNLNSFNFVRMGLEYEAKRQEKILLSGG
VIRQETRRFDEATKTTVLMRVKEGSEDYRYFPEPDLVMLYIDDEWKERVRASIPELPDARRKRYVEEWGLPEYDAKVLTL
TKEMADFFEATVANGADPKLASNWLMVEVSGYLNSEQKELHDIALTPESLAGMIKLIQNGTISSKIAKKVFKELVEHGGD
PEKIVKEKGLVQISDEGALRKIVLEVLDANPQSVEDYKNGKDRALGFLVGQVMKATKGQANPPLVNKLLVEEINKR
>Mature_476_residues
MNFETVIGLEVHVELKTKSKIFSSSPNAFGAPPNTQTNVIDLGYPGVLPVLNRQAVEFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQYDQPLGKNGWIEIEVNGKKKKIGITRIHLEEDAGKLMHTGDGYSLVDFNRQGTPLIEIVSEPDIRS
PEEAYAYLEKLKAIIQYTGVSDCKMEEGSLRCDANISLRPLGSDKFGTKTELKNLNSFNFVRMGLEYEAKRQEKILLSGG
VIRQETRRFDEATKTTVLMRVKEGSEDYRYFPEPDLVMLYIDDEWKERVRASIPELPDARRKRYVEEWGLPEYDAKVLTL
TKEMADFFEATVANGADPKLASNWLMVEVSGYLNSEQKELHDIALTPESLAGMIKLIQNGTISSKIAKKVFKELVEHGGD
PEKIVKEKGLVQISDEGALRKIVLEVLDANPQSVEDYKNGKDRALGFLVGQVMKATKGQANPPLVNKLLVEEINKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=37.979797979798, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=40.133779264214, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=500, Percent_Identity=30, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI45550808, Length=486, Percent_Identity=34.7736625514403, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53609; Mature: 53609

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELKTKSKIFSSSPNAFGAPPNTQTNVIDLGYPGVLPVLNRQAVEFAM
CCCCEEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
KAAMALNCEIATETKFDRKNYFYPDNPKAYQISQYDQPLGKNGWIEIEVNGKKKKIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCEEEEEEEE
IHLEEDAGKLMHTGDGYSLVDFNRQGTPLIEIVSEPDIRSPEEAYAYLEKLKAIIQYTGV
EEEECCCCCEEECCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEEGSLRCDANISLRPLGSDKFGTKTELKNLNSFNFVRMGLEYEAKRQEKILLSGG
CCCEECCCCEEECCCEEEEECCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCC
VIRQETRRFDEATKTTVLMRVKEGSEDYRYFPEPDLVMLYIDDEWKERVRASIPELPDAR
CHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCHH
RKRYVEEWGLPEYDAKVLTLTKEMADFFEATVANGADPKLASNWLMVEVSGYLNSEQKEL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEECCCCCHHHHH
HDIALTPESLAGMIKLIQNGTISSKIAKKVFKELVEHGGDPEKIVKEKGLVQISDEGALR
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHHH
KIVLEVLDANPQSVEDYKNGKDRALGFLVGQVMKATKGQANPPLVNKLLVEEINKR
HHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFETVIGLEVHVELKTKSKIFSSSPNAFGAPPNTQTNVIDLGYPGVLPVLNRQAVEFAM
CCCCEEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
KAAMALNCEIATETKFDRKNYFYPDNPKAYQISQYDQPLGKNGWIEIEVNGKKKKIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCEEEEEEEE
IHLEEDAGKLMHTGDGYSLVDFNRQGTPLIEIVSEPDIRSPEEAYAYLEKLKAIIQYTGV
EEEECCCCCEEECCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEEGSLRCDANISLRPLGSDKFGTKTELKNLNSFNFVRMGLEYEAKRQEKILLSGG
CCCEECCCCEEECCCEEEEECCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCC
VIRQETRRFDEATKTTVLMRVKEGSEDYRYFPEPDLVMLYIDDEWKERVRASIPELPDAR
CHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCHH
RKRYVEEWGLPEYDAKVLTLTKEMADFFEATVANGADPKLASNWLMVEVSGYLNSEQKEL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEECCCCCHHHHH
HDIALTPESLAGMIKLIQNGTISSKIAKKVFKELVEHGGDPEKIVKEKGLVQISDEGALR
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHHH
KIVLEVLDANPQSVEDYKNGKDRALGFLVGQVMKATKGQANPPLVNKLLVEEINKR
HHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA