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The map label for this gene is gatA [H]

Identifier: 138893938

GI number: 138893938

Start: 293759

End: 295216

Strand: Direct

Name: gatA [H]

Synonym: GTNG_0262

Alternate gene names: 138893938

Gene position: 293759-295216 (Clockwise)

Preceding gene: 138893937

Following gene: 138893939

Centisome position: 8.27

GC content: 51.37

Gene sequence:

>1458_bases
ATGTCGCTCTTTGACCATACGGTGTCCGAATTACATACGTTATTGCAGAAAAAAGAAGTGTCCATTTCGGATTTAGTTGA
TGAATCATACCGCCGCATCGGCGAAGTGGAAGAAAAAGTACAAGCGTTTTTAACGTTAAACGAAGAACAAGCGCGGGCGA
AAGCGAAAGAGCTTGACGACAAGCTGGCGAAAGGCGAGGAAACAAACCCGCTGTTTGGCTTGCCGATCGGCATTAAAGAC
AACATTGTCACCAAAGGACTGCGCACGACATGTGCCAGCAAAATTTTATATAACTTTGATCCGATTTACGATGCGACCGT
TATGGAACGGCTGAATGCTGCGGGCGCGATTACGATCGGGAAGCTGAACATGGACGAGTTTGCCATGGGTTCGTCGACGG
AAAACTCCGGGTTCCAGCTGACGCGCAACCCATGGGATTTGGAGCGCGTTCCAGGCGGTTCAAGCGGCGGTTCGGCCGCG
GCAGTGGCGGCAGGCGAAGTGCCGTTTTCGCTAGGATCAGATACGGGTGGTTCGATCCGCCAGCCAGCAGCGTTTTGCGG
AGTTGTCGGATTGAAGCCGACGTACGGTCGCGTTTCGCGTTTCGGGCTCGTCGCGTTTGCGTCGTCGCTTGACCAAATTG
GTCCGATTACGCGCACGGTAGAAGATAATGCATATTTGTTGCAAGTGATCGCTGGCGTTGACCCGATGGATTCGACGTCG
GCGAATATTGACGTGCCAAACTACGTTGAGGCGTTAACAGGCGATATCAAAGGGCTGAAAATCGCTGTGCCAAAAGAATA
TTTAGGCGAAGGCGTCGCCGAAGAAGTACGCCAGTCGGTGCTTGCGGCACTCAGTGTGCTGGAGAAGCTTGGAGCGACAT
GGGAAGAAGTGTCGCTGCCACATTCGAAATATGCCTTGGCGACGTACTATTTGCTTGCTTCGTCGGAAGCATCTGCCAAC
CTTGCCCGCTTTGACGGTGTCCGCTACGGCTACCGGACGGACAATGCGAAAAACTTGATCGATATGTACAAACAAACGCG
GAGCGAAGGGTTCGGCAGCGAAGTCAAACGCCGTATTATGCTTGGGACGTTTGCCTTAAGCTCGGGCTATTACGATGCGT
ATTACAAAAAGGCACAAAAAGTGCGGACGTTAATTAAACGCGACTTTGAAAATGTCTTTGAACAATACGATGTCATCATC
GGTCCGACGACGCCGACGCCAGCGTTTAAAATCGGCGAGAAAACGAGCGATCCGCTGACCATGTATATGAACGATATTTT
AACGATTCCGGTCAACCTTGCGGGTGTACCGGCGATTTCTGTGCCGTGCGGCTTTGTTGATGGCTTGCCGGTCGGCTTGC
AAATTATCGGCAAGCATTTTGATGAAAGCACGGTCTACCGTGTCGCTCACGCCTTTGAACAAGCGACCGATTACCATAAG
CAAAAACCGGTGTTATAA

Upstream 100 bases:

>100_bases
GGAGCCTGGACTGCCGCGTGAAGAAGTGTTGAAAAACGCGCCGGATCAGCAAGACGGCCAATTCCGTGTACCAGCCATTT
TAGAGTAAGGAGGGGGATTT

Downstream 100 bases:

>100_bases
GGGGGGATAGACGATGAATTTTGAAACGGTCATCGGACTTGAGGTGCACGTCGAGCTGAAAACAAAATCGAAAATTTTCT
CAAGCAGCCCGAACGCATTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLFDHTVSELHTLLQKKEVSISDLVDESYRRIGEVEEKVQAFLTLNEEQARAKAKELDDKLAKGEETNPLFGLPIGIKD
NIVTKGLRTTCASKILYNFDPIYDATVMERLNAAGAITIGKLNMDEFAMGSSTENSGFQLTRNPWDLERVPGGSSGGSAA
AVAAGEVPFSLGSDTGGSIRQPAAFCGVVGLKPTYGRVSRFGLVAFASSLDQIGPITRTVEDNAYLLQVIAGVDPMDSTS
ANIDVPNYVEALTGDIKGLKIAVPKEYLGEGVAEEVRQSVLAALSVLEKLGATWEEVSLPHSKYALATYYLLASSEASAN
LARFDGVRYGYRTDNAKNLIDMYKQTRSEGFGSEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKRDFENVFEQYDVII
GPTTPTPAFKIGEKTSDPLTMYMNDILTIPVNLAGVPAISVPCGFVDGLPVGLQIIGKHFDESTVYRVAHAFEQATDYHK
QKPVL

Sequences:

>Translated_485_residues
MSLFDHTVSELHTLLQKKEVSISDLVDESYRRIGEVEEKVQAFLTLNEEQARAKAKELDDKLAKGEETNPLFGLPIGIKD
NIVTKGLRTTCASKILYNFDPIYDATVMERLNAAGAITIGKLNMDEFAMGSSTENSGFQLTRNPWDLERVPGGSSGGSAA
AVAAGEVPFSLGSDTGGSIRQPAAFCGVVGLKPTYGRVSRFGLVAFASSLDQIGPITRTVEDNAYLLQVIAGVDPMDSTS
ANIDVPNYVEALTGDIKGLKIAVPKEYLGEGVAEEVRQSVLAALSVLEKLGATWEEVSLPHSKYALATYYLLASSEASAN
LARFDGVRYGYRTDNAKNLIDMYKQTRSEGFGSEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKRDFENVFEQYDVII
GPTTPTPAFKIGEKTSDPLTMYMNDILTIPVNLAGVPAISVPCGFVDGLPVGLQIIGKHFDESTVYRVAHAFEQATDYHK
QKPVL
>Mature_484_residues
SLFDHTVSELHTLLQKKEVSISDLVDESYRRIGEVEEKVQAFLTLNEEQARAKAKELDDKLAKGEETNPLFGLPIGIKDN
IVTKGLRTTCASKILYNFDPIYDATVMERLNAAGAITIGKLNMDEFAMGSSTENSGFQLTRNPWDLERVPGGSSGGSAAA
VAAGEVPFSLGSDTGGSIRQPAAFCGVVGLKPTYGRVSRFGLVAFASSLDQIGPITRTVEDNAYLLQVIAGVDPMDSTSA
NIDVPNYVEALTGDIKGLKIAVPKEYLGEGVAEEVRQSVLAALSVLEKLGATWEEVSLPHSKYALATYYLLASSEASANL
ARFDGVRYGYRTDNAKNLIDMYKQTRSEGFGSEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKRDFENVFEQYDVIIG
PTTPTPAFKIGEKTSDPLTMYMNDILTIPVNLAGVPAISVPCGFVDGLPVGLQIIGKHFDESTVYRVAHAFEQATDYHKQ
KPVL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=37.8486055776892, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=26.5848670756646, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI166795287, Length=169, Percent_Identity=33.1360946745562, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=426, Percent_Identity=38.7323943661972, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17537465, Length=486, Percent_Identity=27.5720164609054, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17556264, Length=226, Percent_Identity=33.6283185840708, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=249, Percent_Identity=29.3172690763052, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=187, Percent_Identity=36.3636363636364, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=187, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=228, Percent_Identity=28.9473684210526, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=294, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=251, Percent_Identity=28.2868525896414, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=36.3636363636364, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=423, Percent_Identity=32.387706855792, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320448, Length=522, Percent_Identity=22.6053639846743, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=467, Percent_Identity=39.8286937901499, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=28.4552845528455, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=28.4552845528455, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=28.4552845528455, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=28.4552845528455, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=27.378640776699, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=29.3542074363992, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI45550774, Length=486, Percent_Identity=25.3086419753086, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=486, Percent_Identity=25.1028806584362, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648437, Length=486, Percent_Identity=25.1028806584362, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356731, Length=487, Percent_Identity=24.8459958932238, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648441, Length=422, Percent_Identity=25.1184834123223, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648439, Length=422, Percent_Identity=25.1184834123223, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52825; Mature: 52693

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDHTVSELHTLLQKKEVSISDLVDESYRRIGEVEEKVQAFLTLNEEQARAKAKELDD
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KLAKGEETNPLFGLPIGIKDNIVTKGLRTTCASKILYNFDPIYDATVMERLNAAGAITIG
HHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEE
KLNMDEFAMGSSTENSGFQLTRNPWDLERVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIR
CCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
QPAAFCGVVGLKPTYGRVSRFGLVAFASSLDQIGPITRTVEDNAYLLQVIAGVDPMDSTS
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCC
ANIDVPNYVEALTGDIKGLKIAVPKEYLGEGVAEEVRQSVLAALSVLEKLGATWEEVSLP
CCCCCCHHHHHHHCCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
HSKYALATYYLLASSEASANLARFDGVRYGYRTDNAKNLIDMYKQTRSEGFGSEVKRRIM
CHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LGTFALSSGYYDAYYKKAQKVRTLIKRDFENVFEQYDVIIGPTTPTPAFKIGEKTSDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCCCHH
MYMNDILTIPVNLAGVPAISVPCGFVDGLPVGLQIIGKHFDESTVYRVAHAFEQATDYHK
HHHHHHEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QKPVL
CCCCC
>Mature Secondary Structure 
SLFDHTVSELHTLLQKKEVSISDLVDESYRRIGEVEEKVQAFLTLNEEQARAKAKELDD
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KLAKGEETNPLFGLPIGIKDNIVTKGLRTTCASKILYNFDPIYDATVMERLNAAGAITIG
HHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEE
KLNMDEFAMGSSTENSGFQLTRNPWDLERVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIR
CCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
QPAAFCGVVGLKPTYGRVSRFGLVAFASSLDQIGPITRTVEDNAYLLQVIAGVDPMDSTS
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCCCC
ANIDVPNYVEALTGDIKGLKIAVPKEYLGEGVAEEVRQSVLAALSVLEKLGATWEEVSLP
CCCCCCHHHHHHHCCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
HSKYALATYYLLASSEASANLARFDGVRYGYRTDNAKNLIDMYKQTRSEGFGSEVKRRIM
CHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LGTFALSSGYYDAYYKKAQKVRTLIKRDFENVFEQYDVIIGPTTPTPAFKIGEKTSDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCCCHH
MYMNDILTIPVNLAGVPAISVPCGFVDGLPVGLQIIGKHFDESTVYRVAHAFEQATDYHK
HHHHHHEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QKPVL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA