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The map label for this gene is mdeA [H]

Identifier: 138893940

GI number: 138893940

Start: 296847

End: 298151

Strand: Direct

Name: mdeA [H]

Synonym: GTNG_0264

Alternate gene names: 138893940

Gene position: 296847-298151 (Clockwise)

Preceding gene: 138893939

Following gene: 138893941

Centisome position: 8.36

GC content: 52.41

Gene sequence:

>1305_bases
ATGAGTGAAGAACGTGCATTCCGCCCAGAGACGCTCGCCATCCATGCGGGGCAACAACCGGATGCAGAAACAGGTTCGCG
AGCGGTACCGATTTATCAAACGAGTTCGTATGTATTCCGTGACAGTGAGCACGCGGCCAATTTGTTTGGATTGAAAGAGG
AAGGATACATTTATACGCGTATTATGAACCCGACGAACGATGTGCTTGAAAAGCGAATCGCGGCGTTAGAAGGCGGCGTT
GGAGCGCTGGCGCTGTCATCGGGGCAGGCGGCAGTGTTTTATTCGCTCATCAACATCGCCTCGGCGGGTGATGAAATTGT
ATCGTCTTCGTCCATTTACGGCGGCACATACAACTTGTTCGCCCATACGCTGCGCAAATTTGGCATTACGGTGAAGTTTG
TTGACTCGTCTGATCCGGAAAACTTTGAGAAGGCCATTACTGGCAAAACGAAAGCAGTGTTTGCTGAAACGATCGGCAAC
CCGAAAAACGATGTGCTCGACATTGAAGCGGTAGCTGATATCGCTCATCGCCACGGCATTCCGCTCATCGTTGATAACAC
GGTTGCCAGCCCGTACCTGCTGCGGCCGATTGAGTTTGGCGCTGATATCGTTGTCCATTCGGCGACAAAGTTTATCGGCG
GGCACGGCAATTCGATCGGCGGTGTCATTGTCGACAGCGGCAAGTTTGACTGGAAAGGAAGCGGCAAGTTTCCGGAGTTC
ACCGAACCTGATCCGAGTTATCATGGTTTGGTGTATGTCGATGCTGTTGGGGAAGCAGCGTATATCACGAAAGCGCGTAT
TCAGCTGTTGCGCGACTTAGGGGCAGCGCTGTCGCCGTTTAATGCGTTTTTGCTTTTGCAAGGATTAGAGACGCTTCATT
TACGGATGCAGCGTCATAGCGAAAATGCGCTCGCTGTTGCTAAGTTTTTGGAAGAGGAAGAAGCCGTCGAATCCGTCAGC
TATCCAGGGCTTCCAAGCCATCCGTCACATGAACTCGCGAAAAAGTATTTGCCAAACGGGCAAGGTGCGATCGTTACGTT
TGAAATCAAAGGTGGCGTCGAAGCCGGGAAAAAATTGATCGACTCGGTGAAGCTGTTCTCGCATTTGGCCAACATCGGTG
ATTCGAAATCGCTCATCATCCACCCAGCCAGCACGACGCACGAGCAGCTGACCCCGGAAGAACAGCTGTCCGCCGGTGTC
ACCCCAGGTCTTGTTCGCCTGTCTGTCGGCACGGAGTCGATCGACGACATTTTAGAAGACTTACGCCAAGCCATTCGCCA
AAGCCAAACCGTGGCGGTGAAGTAG

Upstream 100 bases:

>100_bases
TGGAAATGATAGATTTTTAGACAAAACCAACTGAATTAGTTGGATATAGACATCAAGAGGAGCGAGAGAGGAAGAATCAA
AACAGAGAGGGATGAGAACG

Downstream 100 bases:

>100_bases
TGGGCATCAACAGATGCGTATACAACACTACCATGAAATTTAGGGATTGAGAAGTGAGGGCTGAACTAAAACGCACGCGC
GTTTTGGATCAGCCCTGCTT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSEERAFRPETLAIHAGQQPDAETGSRAVPIYQTSSYVFRDSEHAANLFGLKEEGYIYTRIMNPTNDVLEKRIAALEGGV
GALALSSGQAAVFYSLINIASAGDEIVSSSSIYGGTYNLFAHTLRKFGITVKFVDSSDPENFEKAITGKTKAVFAETIGN
PKNDVLDIEAVADIAHRHGIPLIVDNTVASPYLLRPIEFGADIVVHSATKFIGGHGNSIGGVIVDSGKFDWKGSGKFPEF
TEPDPSYHGLVYVDAVGEAAYITKARIQLLRDLGAALSPFNAFLLLQGLETLHLRMQRHSENALAVAKFLEEEEAVESVS
YPGLPSHPSHELAKKYLPNGQGAIVTFEIKGGVEAGKKLIDSVKLFSHLANIGDSKSLIIHPASTTHEQLTPEEQLSAGV
TPGLVRLSVGTESIDDILEDLRQAIRQSQTVAVK

Sequences:

>Translated_434_residues
MSEERAFRPETLAIHAGQQPDAETGSRAVPIYQTSSYVFRDSEHAANLFGLKEEGYIYTRIMNPTNDVLEKRIAALEGGV
GALALSSGQAAVFYSLINIASAGDEIVSSSSIYGGTYNLFAHTLRKFGITVKFVDSSDPENFEKAITGKTKAVFAETIGN
PKNDVLDIEAVADIAHRHGIPLIVDNTVASPYLLRPIEFGADIVVHSATKFIGGHGNSIGGVIVDSGKFDWKGSGKFPEF
TEPDPSYHGLVYVDAVGEAAYITKARIQLLRDLGAALSPFNAFLLLQGLETLHLRMQRHSENALAVAKFLEEEEAVESVS
YPGLPSHPSHELAKKYLPNGQGAIVTFEIKGGVEAGKKLIDSVKLFSHLANIGDSKSLIIHPASTTHEQLTPEEQLSAGV
TPGLVRLSVGTESIDDILEDLRQAIRQSQTVAVK
>Mature_433_residues
SEERAFRPETLAIHAGQQPDAETGSRAVPIYQTSSYVFRDSEHAANLFGLKEEGYIYTRIMNPTNDVLEKRIAALEGGVG
ALALSSGQAAVFYSLINIASAGDEIVSSSSIYGGTYNLFAHTLRKFGITVKFVDSSDPENFEKAITGKTKAVFAETIGNP
KNDVLDIEAVADIAHRHGIPLIVDNTVASPYLLRPIEFGADIVVHSATKFIGGHGNSIGGVIVDSGKFDWKGSGKFPEFT
EPDPSYHGLVYVDAVGEAAYITKARIQLLRDLGAALSPFNAFLLLQGLETLHLRMQRHSENALAVAKFLEEEEAVESVSY
PGLPSHPSHELAKKYLPNGQGAIVTFEIKGGVEAGKKLIDSVKLFSHLANIGDSKSLIIHPASTTHEQLTPEEQLSAGVT
PGLVRLSVGTESIDDILEDLRQAIRQSQTVAVK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=418, Percent_Identity=38.2775119617225, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI299473759, Length=418, Percent_Identity=35.1674641148325, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI25453487, Length=417, Percent_Identity=32.8537170263789, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=31.4285714285714, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789383, Length=420, Percent_Identity=26.1904761904762, Blast_Score=138, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=38.0952380952381, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71980708, Length=419, Percent_Identity=35.5608591885441, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=36.9047619047619, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6323333, Length=430, Percent_Identity=53.953488372093, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319307, Length=435, Percent_Identity=36.0919540229885, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321254, Length=452, Percent_Identity=28.0973451327434, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=357, Percent_Identity=27.1708683473389, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=432, Percent_Identity=27.5462962962963, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922554, Length=420, Percent_Identity=35, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46751; Mature: 46620

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEERAFRPETLAIHAGQQPDAETGSRAVPIYQTSSYVFRDSEHAANLFGLKEEGYIYTR
CCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHCCCCCCCEEEEE
IMNPTNDVLEKRIAALEGGVGALALSSGQAAVFYSLINIASAGDEIVSSSSIYGGTYNLF
EECCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
AHTLRKFGITVKFVDSSDPENFEKAITGKTKAVFAETIGNPKNDVLDIEAVADIAHRHGI
HHHHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCC
PLIVDNTVASPYLLRPIEFGADIVVHSATKFIGGHGNSIGGVIVDSGKFDWKGSGKFPEF
CEEEECCCCCCEEECHHHCCCCEEEEHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
TEPDPSYHGLVYVDAVGEAAYITKARIQLLRDLGAALSPFNAFLLLQGLETLHLRMQRHS
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
ENALAVAKFLEEEEAVESVSYPGLPSHPSHELAKKYLPNGQGAIVTFEIKGGVEAGKKLI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
DSVKLFSHLANIGDSKSLIIHPASTTHEQLTPEEQLSAGVTPGLVRLSVGTESIDDILED
HHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCCEEEEECCCHHHHHHHHH
LRQAIRQSQTVAVK
HHHHHHCCCCEECC
>Mature Secondary Structure 
SEERAFRPETLAIHAGQQPDAETGSRAVPIYQTSSYVFRDSEHAANLFGLKEEGYIYTR
CCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHCCCCCCCEEEEE
IMNPTNDVLEKRIAALEGGVGALALSSGQAAVFYSLINIASAGDEIVSSSSIYGGTYNLF
EECCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
AHTLRKFGITVKFVDSSDPENFEKAITGKTKAVFAETIGNPKNDVLDIEAVADIAHRHGI
HHHHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCC
PLIVDNTVASPYLLRPIEFGADIVVHSATKFIGGHGNSIGGVIVDSGKFDWKGSGKFPEF
CEEEECCCCCCEEECHHHCCCCEEEEHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
TEPDPSYHGLVYVDAVGEAAYITKARIQLLRDLGAALSPFNAFLLLQGLETLHLRMQRHS
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
ENALAVAKFLEEEEAVESVSYPGLPSHPSHELAKKYLPNGQGAIVTFEIKGGVEAGKKLI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
DSVKLFSHLANIGDSKSLIIHPASTTHEQLTPEEQLSAGVTPGLVRLSVGTESIDDILED
HHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCCEEEEECCCHHHHHHHHH
LRQAIRQSQTVAVK
HHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]