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The map label for this gene is folC [H]

Identifier: 126696826

GI number: 126696826

Start: 1256923

End: 1258155

Strand: Direct

Name: folC [H]

Synonym: P9301_14881

Alternate gene names: 126696826

Gene position: 1256923-1258155 (Clockwise)

Preceding gene: 126696825

Following gene: 126696829

Centisome position: 76.55

GC content: 30.9

Gene sequence:

>1233_bases
TTGAAAAATGCAAATTTAAAAACTTTTGAATTATTATCACCTAAATATGAAAGGGATAATATCAATTTAGGCTTATCAAG
AATTAAAAAAGCACTTCAAAAACTTGGCAATCCTTGCGAGAATATTCCAGCGATACAAATTATTGGAACCAATGGGAAAG
GATCAATCGCGGCATATTTAGAAAGTATACTTTTTGAATCTAAAAGGAATTTTGGTGTAACGACATCACCTCATCTCTTG
GACATATGCGAGAGAATCAGAGTTAATAAAAAAAATATTAACAAAACTGATTTTGAAAAAATCTATAGATTAATAGAAAA
AAAATTTTCAACATTTGAATTAACTCCTTTTGAAAAAATCATTTGCTGCGCACTGAATTTTTTTGACAATAAAAAAGTTG
AATTGCTCATTCTTGAAGCTGGCTTAGGGGGAAGATTAGACGCGACAACAGCCCATAAATTTAGACCAATAATTGCTATT
GGGAATATTGGCTTAGACCATAAAGAATTTCTCGGAGATACGATTGAAAAAATTGCCGAAGAAAAATTAGCAGTTATTGA
AAAAAACGCAATCGTCATCTCATGCAAACAAAATAGTCAAGTTGAAAATTTAATAACCCAAAAAGTTCAAGAGGTTGGAG
CAGAAATTATCTGGAAAGATGCAATCACAAGCAGCTTTGAGATTGGATTAAAAGGAATTTTTCAAAAGCAAAATGCCTCA
GTAGCTGTTGGTGCAATTGAAGCGCTGAATAATTTGGGATTTAATATAAAAGAGAAATATATATCTGAAGGTCTTAAAAA
GACATCTTGGAAAGGTAGGTTAGAAATAATAAATTATTTGAACAAAGAAATTCTTGTAGATTGTGCGCATAATTATCCTG
CTGCTAAAGCACTCTCTCATGAGCGAAGCAATTGGGAGAATGAAGATAAAGGAATTTATTGGATTTTGGGTGTCCAAAGA
CAAAAAGATATTTACGCAATATTAAAAACACTTCTAAAGAAAAGTGATCACTTATTACTTGTGCCAGTCCCAAACCAACC
TAGTTGGCAATTAAACGATCTCTCACATATTAAAAAAATTGTCCCTCAAAAAACAATTGAATTTAAAACATTTGAACTTG
CCATTGAGTATTTATTTTCCCTAGAAAAATGGCCACCTAATCATCCTGTTCTAACAGGTTCTATTTTTTTAGTTGCTGAA
TTTATTAAATTTGCGAATAAAGAAAAAAATTAA

Upstream 100 bases:

>100_bases
TTGTAAGGATTGTCCCACCATTAGTTATATCTCGAAGAGAAATTAATATTCTTTTGGATAAGCTAAATTTAATTTTTGGA
GAGTTATAGCAATTTAAATT

Downstream 100 bases:

>100_bases
ATTTTAAATTGTTCTTTTACTTCCCAACCTTCCAATTTTCCAGGATTTAAAAGCCCTTTAGGATCAAATCTTAATTTCGC
TTTTACTTGATCTGCATCCA

Product: putative bifunctional dihydrofolate/folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKNANLKTFELLSPKYERDNINLGLSRIKKALQKLGNPCENIPAIQIIGTNGKGSIAAYLESILFESKRNFGVTTSPHLL
DICERIRVNKKNINKTDFEKIYRLIEKKFSTFELTPFEKIICCALNFFDNKKVELLILEAGLGGRLDATTAHKFRPIIAI
GNIGLDHKEFLGDTIEKIAEEKLAVIEKNAIVISCKQNSQVENLITQKVQEVGAEIIWKDAITSSFEIGLKGIFQKQNAS
VAVGAIEALNNLGFNIKEKYISEGLKKTSWKGRLEIINYLNKEILVDCAHNYPAAKALSHERSNWENEDKGIYWILGVQR
QKDIYAILKTLLKKSDHLLLVPVPNQPSWQLNDLSHIKKIVPQKTIEFKTFELAIEYLFSLEKWPPNHPVLTGSIFLVAE
FIKFANKEKN

Sequences:

>Translated_410_residues
MKNANLKTFELLSPKYERDNINLGLSRIKKALQKLGNPCENIPAIQIIGTNGKGSIAAYLESILFESKRNFGVTTSPHLL
DICERIRVNKKNINKTDFEKIYRLIEKKFSTFELTPFEKIICCALNFFDNKKVELLILEAGLGGRLDATTAHKFRPIIAI
GNIGLDHKEFLGDTIEKIAEEKLAVIEKNAIVISCKQNSQVENLITQKVQEVGAEIIWKDAITSSFEIGLKGIFQKQNAS
VAVGAIEALNNLGFNIKEKYISEGLKKTSWKGRLEIINYLNKEILVDCAHNYPAAKALSHERSNWENEDKGIYWILGVQR
QKDIYAILKTLLKKSDHLLLVPVPNQPSWQLNDLSHIKKIVPQKTIEFKTFELAIEYLFSLEKWPPNHPVLTGSIFLVAE
FIKFANKEKN
>Mature_410_residues
MKNANLKTFELLSPKYERDNINLGLSRIKKALQKLGNPCENIPAIQIIGTNGKGSIAAYLESILFESKRNFGVTTSPHLL
DICERIRVNKKNINKTDFEKIYRLIEKKFSTFELTPFEKIICCALNFFDNKKVELLILEAGLGGRLDATTAHKFRPIIAI
GNIGLDHKEFLGDTIEKIAEEKLAVIEKNAIVISCKQNSQVENLITQKVQEVGAEIIWKDAITSSFEIGLKGIFQKQNAS
VAVGAIEALNNLGFNIKEKYISEGLKKTSWKGRLEIINYLNKEILVDCAHNYPAAKALSHERSNWENEDKGIYWILGVQR
QKDIYAILKTLLKKSDHLLLVPVPNQPSWQLNDLSHIKKIVPQKTIEFKTFELAIEYLFSLEKWPPNHPVLTGSIFLVAE
FIKFANKEKN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=320, Percent_Identity=24.6875, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI66932984, Length=320, Percent_Identity=25.3125, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1788654, Length=348, Percent_Identity=28.448275862069, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=314, Percent_Identity=23.8853503184713, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17553150, Length=324, Percent_Identity=23.7654320987654, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17553148, Length=324, Percent_Identity=23.7654320987654, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=429, Percent_Identity=30.5361305361305, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=288, Percent_Identity=29.5138888888889, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322718, Length=348, Percent_Identity=23.2758620689655, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24641571, Length=288, Percent_Identity=27.0833333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24581568, Length=177, Percent_Identity=30.5084745762712, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46533; Mature: 46533

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNANLKTFELLSPKYERDNINLGLSRIKKALQKLGNPCENIPAIQIIGTNGKGSIAAYL
CCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ESILFESKRNFGVTTSPHLLDICERIRVNKKNINKTDFEKIYRLIEKKFSTFELTPFEKI
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHH
ICCALNFFDNKKVELLILEAGLGGRLDATTAHKFRPIIAIGNIGLDHKEFLGDTIEKIAE
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
EKLAVIEKNAIVISCKQNSQVENLITQKVQEVGAEIIWKDAITSSFEIGLKGIFQKQNAS
HHHHHEECCEEEEEECCCCHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHHHCCCCC
VAVGAIEALNNLGFNIKEKYISEGLKKTSWKGRLEIINYLNKEILVDCAHNYPAAKALSH
EEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHH
ERSNWENEDKGIYWILGVQRQKDIYAILKTLLKKSDHLLLVPVPNQPSWQLNDLSHIKKI
HHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
VPQKTIEFKTFELAIEYLFSLEKWPPNHPVLTGSIFLVAEFIKFANKEKN
CCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNANLKTFELLSPKYERDNINLGLSRIKKALQKLGNPCENIPAIQIIGTNGKGSIAAYL
CCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ESILFESKRNFGVTTSPHLLDICERIRVNKKNINKTDFEKIYRLIEKKFSTFELTPFEKI
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHH
ICCALNFFDNKKVELLILEAGLGGRLDATTAHKFRPIIAIGNIGLDHKEFLGDTIEKIAE
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHH
EKLAVIEKNAIVISCKQNSQVENLITQKVQEVGAEIIWKDAITSSFEIGLKGIFQKQNAS
HHHHHEECCEEEEEECCCCHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHHHCCCCC
VAVGAIEALNNLGFNIKEKYISEGLKKTSWKGRLEIINYLNKEILVDCAHNYPAAKALSH
EEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHCCCCHHHHHHH
ERSNWENEDKGIYWILGVQRQKDIYAILKTLLKKSDHLLLVPVPNQPSWQLNDLSHIKKI
HHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
VPQKTIEFKTFELAIEYLFSLEKWPPNHPVLTGSIFLVAEFIKFANKEKN
CCCHHCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]