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The map label for this gene is miaB

Identifier: 126696829

GI number: 126696829

Start: 1261061

End: 1262455

Strand: Direct

Name: miaB

Synonym: P9301_14911

Alternate gene names: 126696829

Gene position: 1261061-1262455 (Clockwise)

Preceding gene: 126696826

Following gene: 126696830

Centisome position: 76.81

GC content: 35.27

Gene sequence:

>1395_bases
GTGCTAACAAAAACAAAATCAGACGAAATAAAAACTCAAAAGGATTCAACTACTGGCAGTTATTGGATAACCACATTTGG
ATGCCAAATGAACAAGGCTGATTCTGAGAGAATGGCAGGGACATTAGAGAAAATGGGATACACCAGAGCAGATAATGAAT
TAAATGCCGATTTGGTCTTGTACAATACATGCACTATTAGAGACAATGCAGAGCAAAAAGTTTATAGCTTTCTAGGAAGA
CAAGCAAAAAGAAAGCACAAAACACCTAGATTAAAACTTGTTGTTGCAGGTTGCCTTGCTCAGCAAGAGGGAGAATCCTT
ACTAAGAAGAGTCCCAGAACTTGACCTAGTCATGGGCCCTCAACATGTAAATAACCTTGAGAATCTTCTGGGGAAAGTTG
ATTTAGGAAATCAAGTTGTTGCTACAGAAGAAACCTTCATATCTGAAGATATAACAAGTGCCAGGAGAGAAAGCTCTATT
TGTGGCTGGGTTAATATCATCTATGGATGTAATGAAAGATGTTCATATTGTGTAGTCCCCTCTGTCAGAGGAAAAGAGCA
ATCAAGATATCCAAATGCGATAAAAAGTGAAATCCAAAAATTAGCTGATGATAATTTCAAAGAAATTACTCTTTTGGGTC
AGAACATTGATGCTTATGGTAGAGACCTTCCAGGAACTACAAAAGAGGGTAGAAAAGAGAATACCCTAACTGATCTTTTG
TATTATATTCATGATGTTAAAGGAATTCGCAGAATAAGATTTGCTACTAGTCATCCAAGATATTTTTCAAAAAGGTTGAT
TCAAGCTTGTTATGAACTTGATAAAGTCTGTGAACATTTCCATATCCCCTTCCAAAGTGGAAATGATGAAATTTTAAAGC
AAATGTCCAGAGGATATTCTATTAAAAAGTATAAAAATATTATCGAGAACATAAGGTCATTAATGCCAGATGCATCAATC
ACAGCTGACGCGATAGTTGCTTTTCCAGGAGAAACCGAGAAACAATACCAAGATACATTAAAGCTTATATCAGAAATTGG
CTTTGATCAGGTGAATACAGCAGCATACTCTGCAAGACCAAATACGCCTGCAGCAGTTTGGACGAATCAACTTTCGGAAG
AGGTAAAAAAAGCTAGATTACAGGAAATTAATGATTTGGTCGAGAAAACTGCTAGGAGTAGAAATCAAAGATATATCGAT
AATATCGAAAGCGTTTTAATTGAGGGTTTAAATCCAAAAAATTCCTCTCAAATTATGGGAAGAACTAGAACAAATCGATT
AACTTTCGTAGAGATTCCCAAAAACATTAACTTTAATTTTTCTTTGGGAGATGAGATAAATGTCAGAATAAATGAAGCAA
GACCTTTTTCTTTAACAGGAGAACTTTCTTTATAA

Upstream 100 bases:

>100_bases
TTCGGGGGTTCGAATCCCCTCGCTCGCCCTCAAAAATATTGCAGCAAAATTATTTAACTTGTAATTTTGAATTAAAGAAT
CATAAAAAATTCCTAGCAAA

Downstream 100 bases:

>100_bases
AAATTTTTTTTAAATGATCGGGGAAAAGAAAAAATGTATTGGGTTAATATTTGGCGGGCATTCCAATGAACATGAAGTAT
CGATATCCTCTGCAAAAACA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLTKTKSDEIKTQKDSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGR
QAKRKHKTPRLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVVATEETFISEDITSARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYSIKKYKNIIENIRSLMPDASI
TADAIVAFPGETEKQYQDTLKLISEIGFDQVNTAAYSARPNTPAAVWTNQLSEEVKKARLQEINDLVEKTARSRNQRYID
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNINFNFSLGDEINVRINEARPFSLTGELSL

Sequences:

>Translated_464_residues
MLTKTKSDEIKTQKDSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGR
QAKRKHKTPRLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVVATEETFISEDITSARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYSIKKYKNIIENIRSLMPDASI
TADAIVAFPGETEKQYQDTLKLISEIGFDQVNTAAYSARPNTPAAVWTNQLSEEVKKARLQEINDLVEKTARSRNQRYID
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNINFNFSLGDEINVRINEARPFSLTGELSL
>Mature_464_residues
MLTKTKSDEIKTQKDSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGR
QAKRKHKTPRLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVVATEETFISEDITSARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYSIKKYKNIIENIRSLMPDASI
TADAIVAFPGETEKQYQDTLKLISEIGFDQVNTAAYSARPNTPAAVWTNQLSEEVKKARLQEINDLVEKTARSRNQRYID
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNINFNFSLGDEINVRINEARPFSLTGELSL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=32.2784810126582, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=32.2784810126582, Blast_Score=246, Evalue=5e-65,
Organism=Homo sapiens, GI93277076, Length=373, Percent_Identity=28.4182305630027, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=36.8539325842697, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=26.7260579064588, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=425, Percent_Identity=34.1176470588235, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=24.8648648648649, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356207, Length=471, Percent_Identity=31.8471337579618, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=24.8917748917749, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PROM0 (A3PED9)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091715.1
- ProteinModelPortal:   A3PED9
- SMR:   A3PED9
- STRING:   A3PED9
- GeneID:   4912718
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_14911
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14333
- BioCyc:   PMAR167546:P9301ORF_1516-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52724; Mature: 52724

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKTKSDEIKTQKDSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVL
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEE
YNTCTIRDNAEQKVYSFLGRQAKRKHKTPRLKLVVAGCLAQQEGESLLRRVPELDLVMGP
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLGKVDLGNQVVATEETFISEDITSARRESSICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
CCCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHH
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IKKYKNIIENIRSLMPDASITADAIVAFPGETEKQYQDTLKLISEIGFDQVNTAAYSARP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCHHCCCCC
NTPAAVWTNQLSEEVKKARLQEINDLVEKTARSRNQRYIDNIESVLIEGLNPKNSSQIMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHC
RTRTNRLTFVEIPKNINFNFSLGDEINVRINEARPFSLTGELSL
CCCCCCEEEEECCCCCCEEEECCCEEEEEEECCCCEEECCCCCC
>Mature Secondary Structure
MLTKTKSDEIKTQKDSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVL
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEE
YNTCTIRDNAEQKVYSFLGRQAKRKHKTPRLKLVVAGCLAQQEGESLLRRVPELDLVMGP
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLGKVDLGNQVVATEETFISEDITSARRESSICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
CCCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHH
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IKKYKNIIENIRSLMPDASITADAIVAFPGETEKQYQDTLKLISEIGFDQVNTAAYSARP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCHHCCCCC
NTPAAVWTNQLSEEVKKARLQEINDLVEKTARSRNQRYIDNIESVLIEGLNPKNSSQIMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHC
RTRTNRLTFVEIPKNINFNFSLGDEINVRINEARPFSLTGELSL
CCCCCCEEEEECCCCCCEEEECCCEEEEEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA