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The map label for this gene is prsA [H]

Identifier: 126696525

GI number: 126696525

Start: 999015

End: 1000010

Strand: Reverse

Name: prsA [H]

Synonym: P9301_11871

Alternate gene names: 126696525

Gene position: 1000010-999015 (Counterclockwise)

Preceding gene: 126696527

Following gene: 126696522

Centisome position: 60.91

GC content: 35.74

Gene sequence:

>996_bases
GTGACAAGTTTTATCACGGCAATGCAGAGTAAAGAGTCGAATTTTAATCTAACAAATAGTAGATTAAGGTTAGTAAGTGG
GACAACTAATCCTAAATTAGCTGAAGAAATTGCATCATACTTAGGGATTGAAAATGTACCTTTAATATCAAAAAGATTTG
CTGATGGAGAACTTTATGTCCAGATTCAACAATCTATTAGAGGTTGCGATGTGTTCCTGATACAACCTACATGTGCTCCT
GTAAACGATAGTTTGATGGAGCTTATGATAATGGTAGACGCGTGCAAGAGAGCGTCTGCGAGACAAATAACAGCTGTAAT
TCCTTATTTTGGATATGCAAGGGCAGATAGAAAAACCTCAGGAAGAGAGTCTATAACTGCAAAACTAACTGCTAATTTGC
TTGAGAAATCAGGAGTTGATAGGGTTCTTGCTATGGATTTACACTCAGCTCAAATTCAAGGATATTTTGACATTCCATGC
GATCATATTTACGGTTCACCTGTGTTAATTGATTATCTAGAAACTTTAGATTTAGAAGAAATAGTTGTCGTCTCTCCGGA
TGTAGGTGGAGTTGCTAGAGCAAGAGCGTTTGCAAAATTAATGAAAGATGCTCCGTTGGCTATTATTGATAAAAGGAGAT
CTGCTCATAATATCGCTGAAAGTTTAACAGTTATTGGTGAAGTTAAAGGTAAGACAGCTATTCTCATAGACGATATGATA
GATACTGGAGGCACAATTTGTTCTGGAGCTAATTTATTAAAACAAGAGGGAGCTAATAGAATATTTGCATGTGCATCGCA
TGCTGTATTCTCTCCTCCTTCTTATGAAAGATTAAGTGCTAAGAATCTATTTGAACAAGTTATTGTGACTAATAGTATAC
CTGTTTTACATAAAGATAATTTTCCGCAGTTAAAAGTTCTTTCTGTTGCGAATATGTTGGGTGAGGCTATTTGGAGAATT
CACGAGGAAAGTTCTGTTAGTTCTATGTTTAGATAA

Upstream 100 bases:

>100_bases
TAGCTTGATTGATTTAACTTTTGAAAAGATATAGAGATTATTTTTTGCGAAATTCTCATAAACGACTACGATAAGATTAT
AGTTTTATGGTGCAAATTTC

Downstream 100 bases:

>100_bases
AAGAAATTATTTTTTCCCTGATCCTTTTAATTTTTTGAGTCTCTCAGTTTCAATTTTGAATTGTTTTTTAATCTTTTCTG
GAACATTATCTGGACACACT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLIDYLETLDLEEIVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
DTGGTICSGANLLKQEGANRIFACASHAVFSPPSYERLSAKNLFEQVIVTNSIPVLHKDNFPQLKVLSVANMLGEAIWRI
HEESSVSSMFR

Sequences:

>Translated_331_residues
MTSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLIDYLETLDLEEIVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
DTGGTICSGANLLKQEGANRIFACASHAVFSPPSYERLSAKNLFEQVIVTNSIPVLHKDNFPQLKVLSVANMLGEAIWRI
HEESSVSSMFR
>Mature_330_residues
TSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYVQIQQSIRGCDVFLIQPTCAPV
NDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCD
HIYGSPVLIDYLETLDLEEIVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMID
TGGTICSGANLLKQEGANRIFACASHAVFSPPSYERLSAKNLFEQVIVTNSIPVLHKDNFPQLKVLSVANMLGEAIWRIH
EESSVSSMFR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=41.3461538461538, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=41.2903225806452, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=41.3461538461538, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=41.2140575079872, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI4506133, Length=356, Percent_Identity=35.9550561797753, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI194018537, Length=333, Percent_Identity=33.6336336336336, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=28.4722222222222, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=28.4722222222222, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=28.4722222222222, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=28.4722222222222, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=49.8392282958199, Blast_Score=324, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI17554702, Length=330, Percent_Identity=40.9090909090909, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71989924, Length=330, Percent_Identity=40.9090909090909, Blast_Score=256, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=41.7197452229299, Blast_Score=254, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=41.9871794871795, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17570245, Length=343, Percent_Identity=31.1953352769679, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320946, Length=317, Percent_Identity=41.6403785488959, Blast_Score=235, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6319403, Length=328, Percent_Identity=38.109756097561, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321776, Length=317, Percent_Identity=38.801261829653, Blast_Score=225, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=42.1319796954315, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=98, Percent_Identity=36.734693877551, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=329, Percent_Identity=40.7294832826748, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI45551540, Length=352, Percent_Identity=38.0681818181818, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24651458, Length=361, Percent_Identity=29.9168975069252, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24651456, Length=361, Percent_Identity=29.9168975069252, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI281362873, Length=361, Percent_Identity=29.9168975069252, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651454, Length=361, Percent_Identity=29.9168975069252, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651462, Length=189, Percent_Identity=35.4497354497354, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24651464, Length=189, Percent_Identity=35.4497354497354, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45552010, Length=189, Percent_Identity=35.4497354497354, Blast_Score=128, Evalue=5e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 36299; Mature: 36168

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYV
CCHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTS
EEHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCCCCC
GRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLIDYLETLDLEE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCEEECCCHHHHCCCCHHHHHHHHCCCCC
IVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHEECCCCEEEEEECHH
DTGGTICSGANLLKQEGANRIFACASHAVFSPPSYERLSAKNLFEQVIVTNSIPVLHKDN
CCCCCEECCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCC
FPQLKVLSVANMLGEAIWRIHEESSVSSMFR
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
TSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYV
CHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMVDACKRASARQITAVIPYFGYARADRKTS
EEHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCCCCC
GRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLIDYLETLDLEE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCEEECCCHHHHCCCCHHHHHHHHCCCCC
IVVVSPDVGGVARARAFAKLMKDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHEECCCCEEEEEECHH
DTGGTICSGANLLKQEGANRIFACASHAVFSPPSYERLSAKNLFEQVIVTNSIPVLHKDN
CCCCCEECCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCC
FPQLKVLSVANMLGEAIWRIHEESSVSSMFR
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]