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The map label for this gene is hisH [H]

Identifier: 126696504

GI number: 126696504

Start: 976275

End: 976892

Strand: Reverse

Name: hisH [H]

Synonym: P9301_11661

Alternate gene names: 126696504

Gene position: 976892-976275 (Counterclockwise)

Preceding gene: 126696505

Following gene: 126696503

Centisome position: 59.5

GC content: 33.01

Gene sequence:

>618_bases
TTGCATAAAATTGGACTAATAGACTATGGGATGGGTAATATTCATTCTGTAACAAAATCTCTAGAAAGTCTTGGAGAAGA
AATCATATTAATTAAAAATTTTAATGACTCTAAGCTTTGTAAAGCGATAATACTCCCTGGGGTTGGAGCCTTTGATCCAG
CTATGAATAATCTCAAAGAGACTGATTTGATAACTGATTTGAAAAATTGGATTAAAAGTGGGAAGTCTTTTTTGGGGATA
TGTTTAGGCCTTCAACTCCTTTTTGAATCAAGTGATGAGGGAGAGGTTCAAGGGCTGGCAATTTTAAAAGGAAATATACA
AAAAATTCCGAATATTGTTAACAAAAGAATTCCACACATGGGTTGGTGCCAACTTTTACCTACAAAACCAAATACTTTAT
TAGAGCTAGAAGAATTAAATAATTGGGTCTATTTTGTACATTCCTATCATGCAATCCCAGATGATTCAAAGATTATTGCA
GCTCAGGTTGATTATGGCTCTGAAAAATTAACTGCAATGATTGAGAATGATAATTTATTGGCCTGTCAATTTCATCCGGA
AAAATCTGGAAAAACCGGTGAAAAACTTTTGAGAAGATGGCTTAGTAATATTCAATAA

Upstream 100 bases:

>100_bases
TGATCTTTAAAGGAGGTCAAAAGGTTGATACCGTTGTTGGTGCTGTGCCAAAAGCAACTCTCTCGAGCACTTTAACTAAG
CATTTATAAATTTAAAAGCT

Downstream 100 bases:

>100_bases
TTGATAATTACTGATGAAGACAAACTTAAGATTAATAGGTGGTAAAAAACTCCAAAGTCCAAATAATTCTTATACCAGAC
CTACAACTTTGAGAGTGAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLKETDLITDLKNWIKSGKSFLGI
CLGLQLLFESSDEGEVQGLAILKGNIQKIPNIVNKRIPHMGWCQLLPTKPNTLLELEELNNWVYFVHSYHAIPDDSKIIA
AQVDYGSEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ

Sequences:

>Translated_205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLKETDLITDLKNWIKSGKSFLGI
CLGLQLLFESSDEGEVQGLAILKGNIQKIPNIVNKRIPHMGWCQLLPTKPNTLLELEELNNWVYFVHSYHAIPDDSKIIA
AQVDYGSEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ
>Mature_205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLKETDLITDLKNWIKSGKSFLGI
CLGLQLLFESSDEGEVQGLAILKGNIQKIPNIVNKRIPHMGWCQLLPTKPNTLLELEELNNWVYFVHSYHAIPDDSKIIA
AQVDYGSEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=36.5482233502538, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=34.7417840375587, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22941; Mature: 22941

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLKE
CCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHH
TDLITDLKNWIKSGKSFLGICLGLQLLFESSDEGEVQGLAILKGNIQKIPNIVNKRIPHM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCC
GWCQLLPTKPNTLLELEELNNWVYFVHSYHAIPDDSKIIAAQVDYGSEKLTAMIENDNLL
CCEEECCCCCCCEEEHHHCCCEEEEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCEE
ACQFHPEKSGKTGEKLLRRWLSNIQ
EEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFNDSKLCKAIILPGVGAFDPAMNNLKE
CCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHH
TDLITDLKNWIKSGKSFLGICLGLQLLFESSDEGEVQGLAILKGNIQKIPNIVNKRIPHM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCC
GWCQLLPTKPNTLLELEELNNWVYFVHSYHAIPDDSKIIAAQVDYGSEKLTAMIENDNLL
CCEEECCCCCCCEEEHHHCCCEEEEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCEE
ACQFHPEKSGKTGEKLLRRWLSNIQ
EEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA