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The map label for this gene is pcaF-2 [H]

Identifier: 126445826

GI number: 126445826

Start: 1834053

End: 1835270

Strand: Direct

Name: pcaF-2 [H]

Synonym: BMA10247_A1902

Alternate gene names: 126445826

Gene position: 1834053-1835270 (Clockwise)

Preceding gene: 126447745

Following gene: 126446349

Centisome position: 77.96

GC content: 72.33

Gene sequence:

>1218_bases
TTGCCGGAATTCGACATGACCGAAGCATTCCTGTGTGACGCAATCCGCACGCCCATCGGCCGCTACGGCGGCGCGCTGGC
GCCGGTGCGGGCCGACGACCTCGGCGCGGTGCCGCTCAAGGCGCTCGTCGAGCGCAACCGCGACGTCGACTGGGCCGCCG
TCGACGACGTGATCTACGGCTGCGCGAATCAGGCCGGCGAGGACAACCGCAACGTCGCGCGCATGTCGCTGCTGCTCGCC
GGCTTGCCGCACGCCGTGCCCGGCGCGACGATCAACCGCCTCTGCGGCTCCGGGATGGACGCGATCGGCCTCGCCGCGCG
CGCGATCAAGGCGGGCGAGGCGAGCCTGATGATCGCCGGCGGCGTCGAGAGCATGAGCCGCGCGCCGTTCGTGACCGGCA
AGGCGACGAGCGCGTTCTCGCGCCAGGCCGAGATCTTCGATACGACCATCGGCTGGCGCTTCGTCAATCCGCTGATGAAA
CAGCGGTACGGCGTCGATTCGATGCCGGAGACGGCCGAAAATGTCGCGACCGATTACCGCGTGAGCCGCGCCGATCAGGA
CGCGTTCGCGCTGCGCAGCCAGCAGAAGGCGGCGCGCGCGCAGGCCGACGGCACGCTCGCGCAGGAGATCGCGCCGGTGA
CGATCGCGCAGAAGCAGGGCGAGCCGCTCGTCGTGGCGCGCGACGAGCATCCGCGCGAGACGACCCTCGAGGCGCTCGCG
AAGCTCAAGGGCGTGGTGCGCCCGGACGGCACGGTGACGGCGGGCAACGCGTCGGGCGTGAACGACGGTGCGTGCGCGCT
GCTGGTGGCGAGCGAGGCGGCGGCGCGCCGCCACGGGCTCGTGCCGCGCGCGCGCGTGCTGGGGATCGCGACGGCGGGCG
TGGAGCCGCGGGTGATGGGGATCGGCCCGGCGCCGGCGACGCAGAAACTGCTCGCGCGGCTCGGGATGACGCTCGACCAG
TTCGACGTGATCGAGCTGAACGAGGCGTTCGCGTCGCAGGGGCTCGCGGTGCTGCGGCTGCTGGGCGTCGCGGACGACGA
TGCGCGGGTGAATCCGAACGGCGGCGCGATCGCGCTCGGGCATCCGCTTGGCGCGTCGGGCGCGCGGCTCGTGACGACCG
CCACGTATCAGCTGCACCGCACGGGCGGCCGCTTCGCGCTCTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGATC
GCGATCGAGCGCGTATAA

Upstream 100 bases:

>100_bases
CCCGCGGAAGCCGGCCAAACGGCATAACCCGTTCGGCCGAGCTGCCGATCGCGCGACCGTTCACTACCATGAATGAACCC
GCCCGCTCGCGGGTTCACCT

Downstream 100 bases:

>100_bases
GCCGGCCCGCGGACGAAGGACGCATCCCGTTCATTTCGAAGAGGCAAGGAGACAGCCGATGTCCTACCAGACGATTCGCA
TCGAAATCGATCAGGCGGCA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MPEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLA
GLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMK
QRYGVDSMPETAENVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALA
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQ
FDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAI
AIERV

Sequences:

>Translated_405_residues
MPEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLA
GLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMK
QRYGVDSMPETAENVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALA
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQ
FDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAI
AIERV
>Mature_404_residues
PEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAG
LPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQ
RYGVDSMPETAENVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALAK
LKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQF
DVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIA
IERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=42.2784810126582, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=39.0428211586902, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=34.2298288508557, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4501853, Length=410, Percent_Identity=37.5609756097561, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.3817330210773, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI194353979, Length=408, Percent_Identity=28.1862745098039, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.3291770573566, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=45.8852867830424, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.9057071960298, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=43.2098765432099, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=31.3868613138686, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=43.4673366834171, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=34.1584158415842, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.9901477832512, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=31.7865429234339, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=29.1044776119403, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=36.1179361179361, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=34.7394540942928, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.142144638404, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=39.1625615763547, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=35.8024691358025, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=30.8962264150943, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24585051, Length=320, Percent_Identity=26.5625, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921506, Length=320, Percent_Identity=25.9375, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42312; Mature: 42181

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYG
CCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
CANQAGEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAG
HHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEC
GVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQRYGVDSMPETAENVATDYR
CHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALA
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCEEEEECCCCCHHHHHHHHH
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMG
HHHCCCCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEE
IGPAPATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALG
CCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEEC
HPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
PEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYG
CCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
CANQAGEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAG
HHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEC
GVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQRYGVDSMPETAENVATDYR
CHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALA
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCEEEEECCCCCHHHHHHHHH
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMG
HHHCCCCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEE
IGPAPATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALG
CCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEEC
HPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]