Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 126207877

GI number: 126207877

Start: 447202

End: 448743

Strand: Direct

Name: leuA [H]

Synonym: APL_0393

Alternate gene names: 126207877

Gene position: 447202-448743 (Clockwise)

Preceding gene: 126207874

Following gene: 126207878

Centisome position: 19.66

GC content: 42.93

Gene sequence:

>1542_bases
ATGAGTAACATTATTATTTTTGATACGACATTACGTGACGGTGAACAATCGCTTAAAGCAAGTTTAACCGTAAAAGAAAA
ACTACAAATTGCGTTAGCATTAGAGCGTTTAGGCGTTGATGTTATGGAAGTCGGTTTTCCGGTTTCATCGGCAGGGGATT
TCGAGTCGGTAAAAACCATTGCTCAACACATTAAAAACAGCCGTGTTTGCGCATTATCCCGCGCAGTGGATAAAGATATT
GATGTGGCGGCGGAAGCATTGAAAGTCGCAGAAGCATTTCGTATTCATACATTTATCGCCAGTTCCGCTATTCATGTTGA
GTCTAAATTAAAACGTTCGTTTGATGATGTCGTTGAAATGGCGGTGCATGCGGTTAAACGTGCCAGAAACTATACCGATG
ATGTGGAGTTTTCCTGTGAAGATGCGGGACGTACCGGTATTGATAACATTTGTCGTATTGTCGAAGCGGCAATCAATGCC
GGTGCGACTACGGTAAATATTCCGGATACGGTCGGTTACTGTTTACCGTATCAGTACGGCGAGATTATCGCCAATGTGAT
GAACCGTGTGCCGAATATTGATAAAGCGGTTGTTTCGGTGCATTGTCATAACGATTTAGGTATGGCGACGGCAAACTCGC
TCACGGCAGTGTTAAATGGTGCGAGACAAATCGAATGTACGATTAACGGTATCGGCGAGCGTGCGGGGAATACCGCATTA
GAAGAAGTGGTCATGGCAATCAAAACTCGCCAAGATATGTTCAATGGTAGAGATACGCGCATTAACACGCAAGAAATTCA
TCGTGTCAGCCAAATGGTCAGCCAATTATGTAATATGCCGATTCAACCGAATAAAGCGATTGTCGGTTCGAATGCCTTTG
CCCATTCATCCGGTATCCACCAAGACGGTATGGTAAAAAATAAAAATACCTATGAAATTATGTCGCCTGAAACCATCGGT
TTGAAGAAAGAAAAATTAAATTTAACCGCCCGTTCAGGACGTGCCGCCGTGAAAAATCATATGAGCGAAATGGGTTATCA
AGAAAACGACTATGATTTGGATAAACTTTATGACGCATTCCTAAAATTGGCGGATAAGAAAGGTCAAGTGTTTGATTACG
ATTTAGAAGCGCTTGCTTTTATTGATATGCAGGCAGGTGATGAAGGCAGATTACATTTAGACGTGATTACTTCGCAAATG
ATTAGCCATTTACCAGCTTCGGCATTTGTTCAAGTCGAATTAGACGGTGAAAAATTAAGCCAAGTTTCCAGTGGCGGTAA
CGGGCCAGTCGATGCGGTCTTTAATGCGATTCTAGCCATTACGGGGATGGATATTAAAATGTTACATTATAACCTTACCG
CAAAAGGCGAAGGTGCGGAAGCATTAGGTCAAGTGGATATTGTGGTTGAACACGAAGGTCGCCGCTTCCACGGTGTCGGT
TTGGCAACCGATATTGTCGAGTCTTCGGCGCGTGCATTAATCCATGTGATCAATGCGATTTATCGAGCGCATAAAGTGGC
GGATTTAAAAGCTAAAAAATAA

Upstream 100 bases:

>100_bases
AAAAAATAGCAAAGAATCGGTGAAGATGGGCATATTTGACTAGTTTATTTCGGTATATTGAATTCGGATAAAAGTGTTGA
GAACACTTAATGGAGAGCTT

Downstream 100 bases:

>100_bases
AGTATTTTAAAAAGATAAGATAAGCGGTCAGATTTGATGGAAATTTTGTAAAAATCCAGTAAAATCTGACCGCTTTCAGT
CATTAAATAAGAAAATATTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVMEVGFPVSSAGDFESVKTIAQHIKNSRVCALSRAVDKDI
DVAAEALKVAEAFRIHTFIASSAIHVESKLKRSFDDVVEMAVHAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINA
GATTVNIPDTVGYCLPYQYGEIIANVMNRVPNIDKAVVSVHCHNDLGMATANSLTAVLNGARQIECTINGIGERAGNTAL
EEVVMAIKTRQDMFNGRDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMVKNKNTYEIMSPETIG
LKKEKLNLTARSGRAAVKNHMSEMGYQENDYDLDKLYDAFLKLADKKGQVFDYDLEALAFIDMQAGDEGRLHLDVITSQM
ISHLPASAFVQVELDGEKLSQVSSGGNGPVDAVFNAILAITGMDIKMLHYNLTAKGEGAEALGQVDIVVEHEGRRFHGVG
LATDIVESSARALIHVINAIYRAHKVADLKAKK

Sequences:

>Translated_513_residues
MSNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVMEVGFPVSSAGDFESVKTIAQHIKNSRVCALSRAVDKDI
DVAAEALKVAEAFRIHTFIASSAIHVESKLKRSFDDVVEMAVHAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINA
GATTVNIPDTVGYCLPYQYGEIIANVMNRVPNIDKAVVSVHCHNDLGMATANSLTAVLNGARQIECTINGIGERAGNTAL
EEVVMAIKTRQDMFNGRDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMVKNKNTYEIMSPETIG
LKKEKLNLTARSGRAAVKNHMSEMGYQENDYDLDKLYDAFLKLADKKGQVFDYDLEALAFIDMQAGDEGRLHLDVITSQM
ISHLPASAFVQVELDGEKLSQVSSGGNGPVDAVFNAILAITGMDIKMLHYNLTAKGEGAEALGQVDIVVEHEGRRFHGVG
LATDIVESSARALIHVINAIYRAHKVADLKAKK
>Mature_512_residues
SNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVMEVGFPVSSAGDFESVKTIAQHIKNSRVCALSRAVDKDID
VAAEALKVAEAFRIHTFIASSAIHVESKLKRSFDDVVEMAVHAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAG
ATTVNIPDTVGYCLPYQYGEIIANVMNRVPNIDKAVVSVHCHNDLGMATANSLTAVLNGARQIECTINGIGERAGNTALE
EVVMAIKTRQDMFNGRDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMVKNKNTYEIMSPETIGL
KKEKLNLTARSGRAAVKNHMSEMGYQENDYDLDKLYDAFLKLADKKGQVFDYDLEALAFIDMQAGDEGRLHLDVITSQMI
SHLPASAFVQVELDGEKLSQVSSGGNGPVDAVFNAILAITGMDIKMLHYNLTAKGEGAEALGQVDIVVEHEGRRFHGVGL
ATDIVESSARALIHVINAIYRAHKVADLKAKK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=503, Percent_Identity=67.3956262425447, Blast_Score=701, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320019, Length=407, Percent_Identity=27.027027027027, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=370, Percent_Identity=27.8378378378378, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=558, Percent_Identity=24.7311827956989, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=24.7211895910781, Blast_Score=126, Evalue=8e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55881; Mature: 55750

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVMEVGFPVSSAGDFESVKTI
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHH
AQHIKNSRVCALSRAVDKDIDVAAEALKVAEAFRIHTFIASSAIHVESKLKRSFDDVVEM
HHHHCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVHAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGYCLPYQYG
HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCHHH
EIIANVMNRVPNIDKAVVSVHCHNDLGMATANSLTAVLNGARQIECTINGIGERAGNTAL
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHH
EEVVMAIKTRQDMFNGRDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGMVKNKNTYEIMSPETIGLKKEKLNLTARSGRAAVKNHMSEMGYQENDYDLDKLYDAF
CCCCCCCCCCEEEECCCCCCCCHHHCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIDMQAGDEGRLHLDVITSQMISHLPASAFVQVELDGEKLS
HHHHCCCCCEEEECCCEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCEEEEEEECHHHHH
QVSSGGNGPVDAVFNAILAITGMDIKMLHYNLTAKGEGAEALGQVDIVVEHEGRRFHGVG
HHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHCCEEEEEECCCCEEECCC
LATDIVESSARALIHVINAIYRAHKVADLKAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVMEVGFPVSSAGDFESVKTI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHH
AQHIKNSRVCALSRAVDKDIDVAAEALKVAEAFRIHTFIASSAIHVESKLKRSFDDVVEM
HHHHCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVHAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAINAGATTVNIPDTVGYCLPYQYG
HHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCHHH
EIIANVMNRVPNIDKAVVSVHCHNDLGMATANSLTAVLNGARQIECTINGIGERAGNTAL
HHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHH
EEVVMAIKTRQDMFNGRDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGMVKNKNTYEIMSPETIGLKKEKLNLTARSGRAAVKNHMSEMGYQENDYDLDKLYDAF
CCCCCCCCCCEEEECCCCCCCCHHHCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIDMQAGDEGRLHLDVITSQMISHLPASAFVQVELDGEKLS
HHHHCCCCCEEEECCCEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCEEEEEEECHHHHH
QVSSGGNGPVDAVFNAILAITGMDIKMLHYNLTAKGEGAEALGQVDIVVEHEGRRFHGVG
HHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHCCEEEEEECCCCEEECCC
LATDIVESSARALIHVINAIYRAHKVADLKAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]