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The map label for this gene is greB [H]

Identifier: 126207870

GI number: 126207870

Start: 439538

End: 440026

Strand: Reverse

Name: greB [H]

Synonym: APL_0386

Alternate gene names: 126207870

Gene position: 440026-439538 (Counterclockwise)

Preceding gene: 126207871

Following gene: 126207862

Centisome position: 19.35

GC content: 43.15

Gene sequence:

>489_bases
GTGGCAAAATCAAATTATATTACGCGTAGCGGATGGCAAGCCTTAGATCAAGAACTTAAATTCTTGTGGAAAGACGAACG
CCCGAAAGTAACGCAAGCGGTATCGGAAGCCGCCGCTTTAGGTGATCGTAGCGAGAATGCCGAATATATCTATGGTAAAC
GCCGGTTACGTGAAATTGATCGCCGAGTTCGTTTTTTATCTAAACGCTTAGAAGTGTTACAAATCGTGGATTATCATCCG
AAACAAGAAGGCAAAGTCTTTTTCGGTGCTTGGGTTGAGCTTGAAAACGAACAAGGAGAAACTAAGCAATATCGGATTGT
CGGTTGTGATGAATTTGATCCGGCTAAGAATTGGATCTCGCTTGATTCGCCGGTAGCCCGAGCGTTAATCGGTAAAGAAG
TGGATGATGAAATTAAGGTGGATACGCCGCTTGGCAAAAGCTTGCTATATATCAACAAAATCTGGTACGAGAAAGAAGGT
CTTTACTAG

Upstream 100 bases:

>100_bases
GGAAGAAATGATGAAAGAATTGCATATCCATTCGCTTATTGCGCTGAACGACGAAGGCAAAGTGTCCGGTATTATGGAAT
TATCAAGTTAAGGATGTTTT

Downstream 100 bases:

>100_bases
ACATAAAATATAAGCGGTCGTTTTTAACGGGAAATTTGCAAAATTCCAGTCAAAAACGACCGCTTGTTTTTAGCACGTCA
AACGAAAATTAGATCGCTTT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MAKSNYITRSGWQALDQELKFLWKDERPKVTQAVSEAAALGDRSENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHP
KQEGKVFFGAWVELENEQGETKQYRIVGCDEFDPAKNWISLDSPVARALIGKEVDDEIKVDTPLGKSLLYINKIWYEKEG
LY

Sequences:

>Translated_162_residues
MAKSNYITRSGWQALDQELKFLWKDERPKVTQAVSEAAALGDRSENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHP
KQEGKVFFGAWVELENEQGETKQYRIVGCDEFDPAKNWISLDSPVARALIGKEVDDEIKVDTPLGKSLLYINKIWYEKEG
LY
>Mature_161_residues
AKSNYITRSGWQALDQELKFLWKDERPKVTQAVSEAAALGDRSENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPK
QEGKVFFGAWVELENEQGETKQYRIVGCDEFDPAKNWISLDSPVARALIGKEVDDEIKVDTPLGKSLLYINKIWYEKEGL
Y

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=60.2564102564103, Blast_Score=189, Evalue=5e-50,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=38.1578947368421, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18946; Mature: 18814

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNYITRSGWQALDQELKFLWKDERPKVTQAVSEAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEQGETKQYRIVGCDEFDPAKNWIS
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCC
LDSPVARALIGKEVDDEIKVDTPLGKSLLYINKIWYEKEGLY
CCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKSNYITRSGWQALDQELKFLWKDERPKVTQAVSEAAALGDRSENAEYIYGKRRLREID
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEQGETKQYRIVGCDEFDPAKNWIS
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCC
LDSPVARALIGKEVDDEIKVDTPLGKSLLYINKIWYEKEGLY
CCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]