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The map label for this gene is glpK [H]

Identifier: 126207859

GI number: 126207859

Start: 424031

End: 425542

Strand: Direct

Name: glpK [H]

Synonym: APL_0375

Alternate gene names: 126207859

Gene position: 424031-425542 (Clockwise)

Preceding gene: 126207858

Following gene: 126207863

Centisome position: 18.64

GC content: 45.77

Gene sequence:

>1512_bases
ATGACTAAAGAATACATTATTGCGTTAGACCAAGGTACGACAAGCTCTCGTGCGGTGTTACTCGACAAAAACGCCAATAT
CGTAGAGGTTTCTCAACGTGAATTTACCCAAATTTATCCGCAAGTCGGTTGGGTGGAACATAATCCGATGGAAATTTGGG
CGACGCAAAGTTCGACATTAAATGAAGTGGTGGCAAAAGCGGGCATTACTTCCGATAAAATTGCGGCAATCGGTATTACC
AACCAACGTGAAACCACGATTGTTTGGGAAAAAGAAACCGGTAAACCGGTTTACAATGCGATTGTATGGCAATGCCGTCG
TACGGCGGATATTTGTGCGAAATTAAAAGAAGACGGGCATGAAGCTTATATTCGTAAAACGACCGGTTTAGTGGTGGACC
CGTATTTCTCCGGTACGAAAGTAAAATGGATTTTAGATAATGTTGAAGGCGCAAGAGAAAAAGCCGAGCTCGGCGAGCTG
TTATTCGGTACGGTAGATACTTGGTTGGTTTGGAAATTAACCCAAGGTCGCGTGCACGTTACCGATTACACAAACGCTTC
CCGTACTATGATGTTCAATATTCATACCAAACAATGGGACGATAAAATGTTGGAATTATTAAATATTCCGCGTTCTATGT
TACCGGAAGTAAGAAATTCATCGGAAGTTTACGGCGAAACCAATATCGGCGGTAAAGGCGGCGTACGTATTCCGGTAGCC
GGTATGGCGGGCGACCAACAAGCGGCGCTTTACGGTCATTTATGCGTAGAAGCCGGTCAGGCGAAAAATACTTACGGTAC
CGGTTGCTTTATGTTGATGAATACCGGTAATGAAGCGGTCGAATCGAAAAACGGTTTGCTGACTACCATCGCATGTAACG
CAAAAGGCGAACCGTGCTATGCGTTGGAAGGTTCGATTTTTATGGGCGGTGCTTCGATTCAGTGGTTACGTGACGAACTT
AAAATCGTACATGACAGTAAAGACTCCGAATATTTTGCAACAAAAGAAGACAGCACAAACGGTGTGTATGTCGTACCGGC
GTTTACCGGCTTAGGCGCGCCGTATTGGGATCCGTATGCTCGAGGTGCGATTTTAGGACTTTCTCGCGGTGCAAACCGTA
ACCATATCGTACGTGCGACATTAGAGTCTATCGCATATCAAACCCGTGACGTATTGGATGCGATGCAATCGGATAGCGGT
AAACATCTTGCGACTTTACGTGTGGACGGCGGTGCGGTTGCCAATAACTTCTTAATGCAATTCCAAGCGGATATTCTCAA
TGCTAACGTAGAACGTCCGGTCGTGCGTGAAGTTACCGCTTTAGGTGCGGCATATCTTGCCGGTCTTGCTGTAGGATTCT
GGAAAGATTTGCAAGAATTACGCGGTAAAGCGTCGATTGAGCGTACCTTTGTTCCGGATGGCGACGAAGCAAAACGTACC
AGACGTTATAAAGGTTGGAAAAAAGCGGTTAAACGTGCGTTAGAATGGGCAAAAGAAGACGCAGAGGAATAA

Upstream 100 bases:

>100_bases
GGACTTGTCGGTGCTTGGGGCTATCGTCGTTTTATCGGTAAAAACTTACCATGTAATTGTAAATAATTGCGTCATTCATT
TAATAGAGAAGGTGATTACT

Downstream 100 bases:

>100_bases
TTTAATTATTCTGTAAGCGGTCTGATTTCTGGTAAATTTTGCAGAAAATACGACCGCTTATTATAGAAAAACAAAGGGCT
TCAAATAATTTGAAGCCCTT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTKEYIIALDQGTTSSRAVLLDKNANIVEVSQREFTQIYPQVGWVEHNPMEIWATQSSTLNEVVAKAGITSDKIAAIGIT
NQRETTIVWEKETGKPVYNAIVWQCRRTADICAKLKEDGHEAYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAELGEL
LFGTVDTWLVWKLTQGRVHVTDYTNASRTMMFNIHTKQWDDKMLELLNIPRSMLPEVRNSSEVYGETNIGGKGGVRIPVA
GMAGDQQAALYGHLCVEAGQAKNTYGTGCFMLMNTGNEAVESKNGLLTTIACNAKGEPCYALEGSIFMGGASIQWLRDEL
KIVHDSKDSEYFATKEDSTNGVYVVPAFTGLGAPYWDPYARGAILGLSRGANRNHIVRATLESIAYQTRDVLDAMQSDSG
KHLATLRVDGGAVANNFLMQFQADILNANVERPVVREVTALGAAYLAGLAVGFWKDLQELRGKASIERTFVPDGDEAKRT
RRYKGWKKAVKRALEWAKEDAEE

Sequences:

>Translated_503_residues
MTKEYIIALDQGTTSSRAVLLDKNANIVEVSQREFTQIYPQVGWVEHNPMEIWATQSSTLNEVVAKAGITSDKIAAIGIT
NQRETTIVWEKETGKPVYNAIVWQCRRTADICAKLKEDGHEAYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAELGEL
LFGTVDTWLVWKLTQGRVHVTDYTNASRTMMFNIHTKQWDDKMLELLNIPRSMLPEVRNSSEVYGETNIGGKGGVRIPVA
GMAGDQQAALYGHLCVEAGQAKNTYGTGCFMLMNTGNEAVESKNGLLTTIACNAKGEPCYALEGSIFMGGASIQWLRDEL
KIVHDSKDSEYFATKEDSTNGVYVVPAFTGLGAPYWDPYARGAILGLSRGANRNHIVRATLESIAYQTRDVLDAMQSDSG
KHLATLRVDGGAVANNFLMQFQADILNANVERPVVREVTALGAAYLAGLAVGFWKDLQELRGKASIERTFVPDGDEAKRT
RRYKGWKKAVKRALEWAKEDAEE
>Mature_502_residues
TKEYIIALDQGTTSSRAVLLDKNANIVEVSQREFTQIYPQVGWVEHNPMEIWATQSSTLNEVVAKAGITSDKIAAIGITN
QRETTIVWEKETGKPVYNAIVWQCRRTADICAKLKEDGHEAYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAELGELL
FGTVDTWLVWKLTQGRVHVTDYTNASRTMMFNIHTKQWDDKMLELLNIPRSMLPEVRNSSEVYGETNIGGKGGVRIPVAG
MAGDQQAALYGHLCVEAGQAKNTYGTGCFMLMNTGNEAVESKNGLLTTIACNAKGEPCYALEGSIFMGGASIQWLRDELK
IVHDSKDSEYFATKEDSTNGVYVVPAFTGLGAPYWDPYARGAILGLSRGANRNHIVRATLESIAYQTRDVLDAMQSDSGK
HLATLRVDGGAVANNFLMQFQADILNANVERPVVREVTALGAAYLAGLAVGFWKDLQELRGKASIERTFVPDGDEAKRTR
RYKGWKKAVKRALEWAKEDAEE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=509, Percent_Identity=44.9901768172888, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4504007, Length=509, Percent_Identity=44.9901768172888, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=44.6393762183236, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI41393575, Length=509, Percent_Identity=43.614931237721, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=33.8582677165354, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=77.9116465863454, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=499, Percent_Identity=24.4488977955912, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787789, Length=506, Percent_Identity=25.098814229249, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790005, Length=496, Percent_Identity=22.3790322580645, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789138, Length=514, Percent_Identity=22.1789883268482, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=42.6587301587302, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71986140, Length=525, Percent_Identity=24.5714285714286, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=28.9036544850498, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=557, Percent_Identity=35.3680430879713, Blast_Score=315, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17864214, Length=514, Percent_Identity=42.6070038910506, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=40.1197604790419, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=40.1197604790419, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=40.1197604790419, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=40.1197604790419, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=40.1197604790419, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24654573, Length=456, Percent_Identity=44.078947368421, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=34.3511450381679, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24652871, Length=502, Percent_Identity=34.2629482071713, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=26.937984496124, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=26.0869565217391, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=28.6532951289398, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45552909, Length=287, Percent_Identity=26.1324041811847, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55664; Mature: 55533

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEYIIALDQGTTSSRAVLLDKNANIVEVSQREFTQIYPQVGWVEHNPMEIWATQSSTL
CCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCEECCCEEEEEECCHHH
NEVVAKAGITSDKIAAIGITNQRETTIVWEKETGKPVYNAIVWQCRRTADICAKLKEDGH
HHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EAYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAELGELLFGTVDTWLVWKLTQGRVHV
EEEEEECCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEE
TDYTNASRTMMFNIHTKQWDDKMLELLNIPRSMLPEVRNSSEVYGETNIGGKGGVRIPVA
EECCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
GMAGDQQAALYGHLCVEAGQAKNTYGTGCFMLMNTGNEAVESKNGLLTTIACNAKGEPCY
CCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCCCEE
ALEGSIFMGGASIQWLRDELKIVHDSKDSEYFATKEDSTNGVYVVPAFTGLGAPYWDPYA
EECCCEEECCCHHHHHHHHHHEEECCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCHHH
RGAILGLSRGANRNHIVRATLESIAYQTRDVLDAMQSDSGKHLATLRVDGGAVANNFLMQ
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHH
FQADILNANVERPVVREVTALGAAYLAGLAVGFWKDLQELRGKASIERTFVPDGDEAKRT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
RRYKGWKKAVKRALEWAKEDAEE
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEYIIALDQGTTSSRAVLLDKNANIVEVSQREFTQIYPQVGWVEHNPMEIWATQSSTL
CCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCEECCCEEEEEECCHHH
NEVVAKAGITSDKIAAIGITNQRETTIVWEKETGKPVYNAIVWQCRRTADICAKLKEDGH
HHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EAYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAELGELLFGTVDTWLVWKLTQGRVHV
EEEEEECCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEE
TDYTNASRTMMFNIHTKQWDDKMLELLNIPRSMLPEVRNSSEVYGETNIGGKGGVRIPVA
EECCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
GMAGDQQAALYGHLCVEAGQAKNTYGTGCFMLMNTGNEAVESKNGLLTTIACNAKGEPCY
CCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCCCEE
ALEGSIFMGGASIQWLRDELKIVHDSKDSEYFATKEDSTNGVYVVPAFTGLGAPYWDPYA
EECCCEEECCCHHHHHHHHHHEEECCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCHHH
RGAILGLSRGANRNHIVRATLESIAYQTRDVLDAMQSDSGKHLATLRVDGGAVANNFLMQ
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHH
FQADILNANVERPVVREVTALGAAYLAGLAVGFWKDLQELRGKASIERTFVPDGDEAKRT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
RRYKGWKKAVKRALEWAKEDAEE
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]