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The map label for this gene is glpF [H]

Identifier: 126207858

GI number: 126207858

Start: 423214

End: 423996

Strand: Direct

Name: glpF [H]

Synonym: APL_0374

Alternate gene names: 126207858

Gene position: 423214-423996 (Clockwise)

Preceding gene: 126207857

Following gene: 126207859

Centisome position: 18.61

GC content: 47.64

Gene sequence:

>783_bases
ATGGAAAGATCGTTAAAAGGTGCTTGTATCGCAGAATTTATCGGTACGGGTTTAATTATTTTCTTTGGGGTAGGTTGTGT
TGCCGCCGCACAATTAGCCGGTGCGACATTCGGTTTATGGGAAATCTCCATTATGTGGGGCGTGGGGGTTGCTTTGGCGG
TATATACCACCGCAGGCGTATCCGGTGCGCATTTAAATCCGGCGGTAACCGTCGCACTTTGGAAGTTTGCGTGTTTTGAC
GGAAAAAAAGTACTGCCTTATATCATTTCCCAATTTTTAGGTGCTTTTGCCGCAGCGGCGTTAGTTTATTTCCTTTATAA
AGATCTCTTTGCCGCAACCGAAGCCGCTAAAAATATTGTACGCGGCGAAGGCGTCGGACTTGCCGGTGTTTTCTCTACCT
ATCCTCATCAACATATCAGCGTATTACAAGCCTTTTGTGTCGAAGCGGTCATTACCATGGCGTTAGTCGCATTAATTTTA
GCTTTAACCGATGACGGTAACGGCGTACCGAGAGGTCCGATGGCTCCGTTATTAATCGGTTTATTGATCGCTGCGATCGG
CGGTGCTTTCGGTCCTTTAACCGGCTTTGCAATGAATCCGGCGCGTGACTTCGGTCCGAAAGCCTTTGCATTCTTAGCCG
GTTGGGGTCAAGTTGCCTTTACCGGTGCACGCGATATTCCGTATTTCCTCGTTCCTTTGATTGCGCCGATTGTCGGCGGA
CTTGTCGGTGCTTGGGGCTATCGTCGTTTTATCGGTAAAAACTTACCATGTAATTGTAAATAA

Upstream 100 bases:

>100_bases
TGCGAAAAATGTTCGTTTTTATATAGTTCGCATCGAAAACGTTTGCGTTTTTCAACATTCAATAGCGTAATTTACTTTTC
GTTATCCGTAGGAGGACTAT

Downstream 100 bases:

>100_bases
TTGCGTCATTCATTTAATAGAGAAGGTGATTACTATGACTAAAGAATACATTATTGCGTTAGACCAAGGTACGACAAGCT
CTCGTGCGGTGTTACTCGAC

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MERSLKGACIAEFIGTGLIIFFGVGCVAAAQLAGATFGLWEISIMWGVGVALAVYTTAGVSGAHLNPAVTVALWKFACFD
GKKVLPYIISQFLGAFAAAALVYFLYKDLFAATEAAKNIVRGEGVGLAGVFSTYPHQHISVLQAFCVEAVITMALVALIL
ALTDDGNGVPRGPMAPLLIGLLIAAIGGAFGPLTGFAMNPARDFGPKAFAFLAGWGQVAFTGARDIPYFLVPLIAPIVGG
LVGAWGYRRFIGKNLPCNCK

Sequences:

>Translated_260_residues
MERSLKGACIAEFIGTGLIIFFGVGCVAAAQLAGATFGLWEISIMWGVGVALAVYTTAGVSGAHLNPAVTVALWKFACFD
GKKVLPYIISQFLGAFAAAALVYFLYKDLFAATEAAKNIVRGEGVGLAGVFSTYPHQHISVLQAFCVEAVITMALVALIL
ALTDDGNGVPRGPMAPLLIGLLIAAIGGAFGPLTGFAMNPARDFGPKAFAFLAGWGQVAFTGARDIPYFLVPLIAPIVGG
LVGAWGYRRFIGKNLPCNCK
>Mature_260_residues
MERSLKGACIAEFIGTGLIIFFGVGCVAAAQLAGATFGLWEISIMWGVGVALAVYTTAGVSGAHLNPAVTVALWKFACFD
GKKVLPYIISQFLGAFAAAALVYFLYKDLFAATEAAKNIVRGEGVGLAGVFSTYPHQHISVLQAFCVEAVITMALVALIL
ALTDDGNGVPRGPMAPLLIGLLIAAIGGAFGPLTGFAMNPARDFGPKAFAFLAGWGQVAFTGARDIPYFLVPLIAPIVGG
LVGAWGYRRFIGKNLPCNCK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=258, Percent_Identity=38.3720930232558, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI4826645, Length=255, Percent_Identity=38.0392156862745, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4502187, Length=256, Percent_Identity=33.984375, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI22538420, Length=254, Percent_Identity=35.0393700787402, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI310133356, Length=204, Percent_Identity=31.8627450980392, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310114181, Length=204, Percent_Identity=31.8627450980392, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI45446752, Length=204, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI86792455, Length=207, Percent_Identity=33.8164251207729, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502179, Length=242, Percent_Identity=32.2314049586777, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI6912506, Length=241, Percent_Identity=31.9502074688797, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI37694062, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1790362, Length=258, Percent_Identity=72.0930232558139, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1787101, Length=194, Percent_Identity=30.9278350515464, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=258, Percent_Identity=35.6589147286822, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71992966, Length=252, Percent_Identity=36.1111111111111, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564052, Length=248, Percent_Identity=35.4838709677419, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=248, Percent_Identity=35.4838709677419, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=261, Percent_Identity=32.5670498084291, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531429, Length=261, Percent_Identity=32.5670498084291, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17544068, Length=255, Percent_Identity=35.6862745098039, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71992961, Length=252, Percent_Identity=32.9365079365079, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17558372, Length=269, Percent_Identity=29.7397769516729, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=40.2843601895735, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322985, Length=233, Percent_Identity=30.9012875536481, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762346, Length=201, Percent_Identity=33.8308457711443, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762348, Length=201, Percent_Identity=33.8308457711443, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130305, Length=201, Percent_Identity=33.8308457711443, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762344, Length=201, Percent_Identity=33.8308457711443, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550503, Length=245, Percent_Identity=27.7551020408163, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652747, Length=240, Percent_Identity=32.9166666666667, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551084, Length=240, Percent_Identity=32.9166666666667, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27080; Mature: 27080

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]