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The map label for this gene is potD2 [H]

Identifier: 126207852

GI number: 126207852

Start: 413401

End: 414483

Strand: Direct

Name: potD2 [H]

Synonym: APL_0368

Alternate gene names: 126207852

Gene position: 413401-414483 (Clockwise)

Preceding gene: 126207846

Following gene: 126207853

Centisome position: 18.18

GC content: 39.8

Gene sequence:

>1083_bases
ATGAAAAAATTAGCGGGTTTATTTGCAGCAGGTTTAGCGACAGTTGCATTAACAGCGTGTAATGAAGAAAAGCCAAAAGC
GGCTGAAGCAGCGGCTCAACCGGCAGCAGCGGGAACAGTTCACCTTTATACTTGGACTGAATATGTGCCTGAAGGCTTGT
TAGATGAATTTACAAAGCAAACCGGTATCAAAGTAGAGGTTTCAAGCCTTGAATCTAACGAAACCATGTATGCGAAATTA
AAATTACAAGGTAAAGACGGCGGTTACGATGTTATCGCACCTTCTAACTACTTCGTTTCAAAAATGGCGAAAGAAGGTAT
GTTAGCGGAATTAGATCACGCAAAACTTCCTGTAATCAAAGAGTTAAACCAAGATTGGTTAAACAAACCTTATGACCAAG
GTAACAAATACTCTTTACCGCAATTATTAGGTGCACCGGGTATCGCATTTAACTCAAATGACTATAAGGGCGATGCGTTC
ACTTCTTGGGGTGATTTATGGAAACCTGAGTTTGCGAATAAAGTACAATTATTAGATGACGCACGTGAAGTATTTAACAT
TGCGTTATTAAAATTAGGTAAAAACCCTAATACAACCAATCCGGAAGAGATTAAAGCGGCTTACGAAGAGTTAAGAAAAT
TACGTCCAAACGTACTTTCTTTCACTTCAGACAACCCAGCGAACTCATTTATCGCAGGTGAAGTATCTGTAGGTCAATTA
TGGAACGGTTCTGTACGTATTGCGAAAAAAGAACAAGCGCCGGTAAACATGGTGTTCCCAAAAGAAGGTCCTGTACTTTG
GGTTGATACGTTAGCCATTCCGGCGAATGCGAAAAACAAAGAAAATGCGCATAAGTTAATCAACTACTTATTAAGCGCAC
CGGTTGCGGAAAAATTAACGTTAGAAATCGGTTATCCGACTTCAAACGTAGAAGCGTTAAAAACATTACCAAAAGAGATT
ACCGAAGATCCGGCAATCTATCCGACAGCTGATGTGTTAAAAGCGGCACAATGGCAAGACGATGTAGGTAATGCAATCGA
ACTTTACGAAAAATATTACCAAGAGTTAAAAGCGGCGAAATAA

Upstream 100 bases:

>100_bases
TTGCAAAATCTAGGGGGTGGCTTAAAATCGCCCTCGTCTTTTAAGCCCTTAGGCAAGGCTATATTTTTTACTCATCTTTT
CTTTAACGGAGAAAACTTAA

Downstream 100 bases:

>100_bases
TTCGTTAGCCTAACGGATAAAGCCCTCGAGAAGCGATTTTCGAGGGCTTTTCGTTTTTACAAGCGGTCTAATTTTGCAAA
ATTTTTGCGAAAAACAGCCG

Product: spermidine/putrescine-binding periplasmic protein 1 precursor

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKLAGLFAAGLATVALTACNEEKPKAAEAAAQPAAAGTVHLYTWTEYVPEGLLDEFTKQTGIKVEVSSLESNETMYAKL
KLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAKLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNSNDYKGDAF
TSWGDLWKPEFANKVQLLDDAREVFNIALLKLGKNPNTTNPEEIKAAYEELRKLRPNVLSFTSDNPANSFIAGEVSVGQL
WNGSVRIAKKEQAPVNMVFPKEGPVLWVDTLAIPANAKNKENAHKLINYLLSAPVAEKLTLEIGYPTSNVEALKTLPKEI
TEDPAIYPTADVLKAAQWQDDVGNAIELYEKYYQELKAAK

Sequences:

>Translated_360_residues
MKKLAGLFAAGLATVALTACNEEKPKAAEAAAQPAAAGTVHLYTWTEYVPEGLLDEFTKQTGIKVEVSSLESNETMYAKL
KLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAKLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNSNDYKGDAF
TSWGDLWKPEFANKVQLLDDAREVFNIALLKLGKNPNTTNPEEIKAAYEELRKLRPNVLSFTSDNPANSFIAGEVSVGQL
WNGSVRIAKKEQAPVNMVFPKEGPVLWVDTLAIPANAKNKENAHKLINYLLSAPVAEKLTLEIGYPTSNVEALKTLPKEI
TEDPAIYPTADVLKAAQWQDDVGNAIELYEKYYQELKAAK
>Mature_360_residues
MKKLAGLFAAGLATVALTACNEEKPKAAEAAAQPAAAGTVHLYTWTEYVPEGLLDEFTKQTGIKVEVSSLESNETMYAKL
KLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAKLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNSNDYKGDAF
TSWGDLWKPEFANKVQLLDDAREVFNIALLKLGKNPNTTNPEEIKAAYEELRKLRPNVLSFTSDNPANSFIAGEVSVGQL
WNGSVRIAKKEQAPVNMVFPKEGPVLWVDTLAIPANAKNKENAHKLINYLLSAPVAEKLTLEIGYPTSNVEALKTLPKEI
TEDPAIYPTADVLKAAQWQDDVGNAIELYEKYYQELKAAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=331, Percent_Identity=53.4743202416918, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1787078, Length=352, Percent_Identity=33.8068181818182, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39579; Mature: 39579

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLAGLFAAGLATVALTACNEEKPKAAEAAAQPAAAGTVHLYTWTEYVPEGLLDEFTKQ
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
TGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAKLPVIK
CCCEEEEECCCCCCEEEEEEEEECCCCCEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHH
ELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNSNDYKGDAFTSWGDLWKPEFANKVQLLDD
HHCHHHCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHH
AREVFNIALLKLGKNPNTTNPEEIKAAYEELRKLRPNVLSFTSDNPANSFIAGEVSVGQL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCEEECCC
WNGSVRIAKKEQAPVNMVFPKEGPVLWVDTLAIPANAKNKENAHKLINYLLSAPVAEKLT
CCCCEEEEECCCCCEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEE
LEIGYPTSNVEALKTLPKEITEDPAIYPTADVLKAAQWQDDVGNAIELYEKYYQELKAAK
EEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLAGLFAAGLATVALTACNEEKPKAAEAAAQPAAAGTVHLYTWTEYVPEGLLDEFTKQ
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
TGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYFVSKMAKEGMLAELDHAKLPVIK
CCCEEEEECCCCCCEEEEEEEEECCCCCEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHH
ELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNSNDYKGDAFTSWGDLWKPEFANKVQLLDD
HHCHHHCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHH
AREVFNIALLKLGKNPNTTNPEEIKAAYEELRKLRPNVLSFTSDNPANSFIAGEVSVGQL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCEEECCC
WNGSVRIAKKEQAPVNMVFPKEGPVLWVDTLAIPANAKNKENAHKLINYLLSAPVAEKLT
CCCCEEEEECCCCCEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEE
LEIGYPTSNVEALKTLPKEITEDPAIYPTADVLKAAQWQDDVGNAIELYEKYYQELKAAK
EEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]