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The map label for this gene is tolB

Identifier: 126207787

GI number: 126207787

Start: 335598

End: 336878

Strand: Direct

Name: tolB

Synonym: APL_0303

Alternate gene names: 126207787

Gene position: 335598-336878 (Clockwise)

Preceding gene: 126207786

Following gene: 126207788

Centisome position: 14.75

GC content: 46.21

Gene sequence:

>1281_bases
ATGAAATTAAAATCTCGTTTTACGAGTATTATCGGCGTTATCACCCTCTTTTTCAGCCAAACGGTAACGGCTGAATCAGA
TGTAGTAATTTCTGTTGATGAAGGTGTAAGTATGGCGCAACCTATTGCGGTTGTTCCTTTTAAAGCAAACGGCGGTGTGC
CGGCTGACGTAGGACAAATTATTGCCGACGACTTACGTAATAGCGGCAAGTTCACTCCCGTAGAACGTTCTAAATTACCT
GCACAGCCGGGTTCTGCAGCGGAAGTAAATTCACAACAATGGACGGATATCGGTGTCGATTCGGTTGTTGTAGGGCAAGT
GACGCCAACCGGCGGCGGTTACAATGTTGCTTATCAGTTAGTAGATACGTTAAGTAATCCGGCAACCGTACTGGCTCAAG
GCGCATTTAACGTACCTGCGGCGCAAATTCGTCAAGGCGCGCATACGGTAAGTGACCAAGTATTTGAGAAAATTACACAA
ATTCGAGGCGCATTCAGAACAAAAATTGCATATGTAGTGCAACGTGGTGTATCATCATACGAATTGAGAGTATCCGATTA
CGACGGTTATAATGCGTTCACGGTTGTGAAGAGTAAAGAGCCGTTGATGTCGCCAGAATGGTCTCCGGACGGTAGTCGTT
TAGCTTATGTAACATTTGAAAATAAAAAGGCACAAGTTGTCGTCCACGATTTACGTTCGGGTTCTCGTCGTGTCGTAGCG
GCGTTACGCGGACATAACGGTGCGCCGGCGTTCTCTCCGGACGGTTCTCGTATTGCGTTCGCATCGAATCAAGACGGTGA
GTTGGATATCTATGTTGTCGGTGCGAATGGCGGTAAACCGTCGAAATTAACTGCAAATGCGGGTAATAATACCGAACCGA
GTTGGTCTCCGGACGGAAGTACGATTTACTTCACTTCGGATCGTGCAGGTTCTCCGCAAGTATATAGAATGGGTTCATCA
GGCGGCGGCGCAAGCCCTATGGGCGGTAGCGGTAGCTATAACGCTAAAGTATCGTCTGATGGTAAGAATTTAATCATGAT
TGCCGGTGATAAAGTAGTAAAACGCGATCTCGCTTCGGGTGGCACGGAAGTATTGAGTTCAACGTTCTTAGATGAAAGTC
CAAGTATTTCTCCAAATGGTATTATGGTTATTTATAGCTCTACCAAAGGTACGAGCAAAGTGCTACAATTGGTGTCCGCA
GATGGCCGTTTCAAAGCTAACTTGCCGGGAGCAGGTGGACAATTTAAGTTTCCTGCTTGGTCACCGTATTTGACTAAATA
A

Upstream 100 bases:

>100_bases
GAAATGTTCCGAGAGCGCCAAATGAAGAGACTTATAACAAATATAAATCTCCGATTATTAAATTCGGTTTAAAAGTTTTA
TAACAACCAAGAGGTTAAAA

Downstream 100 bases:

>100_bases
AATAAAATTCTTTTAGGAGCAACAAATGAAAAAACTAGCTAAAGTATTGATGATTGCGGCACCAGCATTCGTATTAGCAG
CTTGCAGCAGCTCATCAGAC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLKSRFTSIIGVITLFFSQTVTAESDVVISVDEGVSMAQPIAVVPFKANGGVPADVGQIIADDLRNSGKFTPVERSKLP
AQPGSAAEVNSQQWTDIGVDSVVVGQVTPTGGGYNVAYQLVDTLSNPATVLAQGAFNVPAAQIRQGAHTVSDQVFEKITQ
IRGAFRTKIAYVVQRGVSSYELRVSDYDGYNAFTVVKSKEPLMSPEWSPDGSRLAYVTFENKKAQVVVHDLRSGSRRVVA
ALRGHNGAPAFSPDGSRIAFASNQDGELDIYVVGANGGKPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMGSS
GGGASPMGGSGSYNAKVSSDGKNLIMIAGDKVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKGTSKVLQLVSA
DGRFKANLPGAGGQFKFPAWSPYLTK

Sequences:

>Translated_426_residues
MKLKSRFTSIIGVITLFFSQTVTAESDVVISVDEGVSMAQPIAVVPFKANGGVPADVGQIIADDLRNSGKFTPVERSKLP
AQPGSAAEVNSQQWTDIGVDSVVVGQVTPTGGGYNVAYQLVDTLSNPATVLAQGAFNVPAAQIRQGAHTVSDQVFEKITQ
IRGAFRTKIAYVVQRGVSSYELRVSDYDGYNAFTVVKSKEPLMSPEWSPDGSRLAYVTFENKKAQVVVHDLRSGSRRVVA
ALRGHNGAPAFSPDGSRIAFASNQDGELDIYVVGANGGKPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMGSS
GGGASPMGGSGSYNAKVSSDGKNLIMIAGDKVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKGTSKVLQLVSA
DGRFKANLPGAGGQFKFPAWSPYLTK
>Mature_426_residues
MKLKSRFTSIIGVITLFFSQTVTAESDVVISVDEGVSMAQPIAVVPFKANGGVPADVGQIIADDLRNSGKFTPVERSKLP
AQPGSAAEVNSQQWTDIGVDSVVVGQVTPTGGGYNVAYQLVDTLSNPATVLAQGAFNVPAAQIRQGAHTVSDQVFEKITQ
IRGAFRTKIAYVVQRGVSSYELRVSDYDGYNAFTVVKSKEPLMSPEWSPDGSRLAYVTFENKKAQVVVHDLRSGSRRVVA
ALRGHNGAPAFSPDGSRIAFASNQDGELDIYVVGANGGKPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMGSS
GGGASPMGGSGSYNAKVSSDGKNLIMIAGDKVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKGTSKVLQLVSA
DGRFKANLPGAGGQFKFPAWSPYLTK

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=433, Percent_Identity=54.0415704387991, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_ACTP2 (A3MZ21)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053012.1
- ProteinModelPortal:   A3MZ21
- SMR:   A3MZ21
- STRING:   A3MZ21
- GeneID:   4848550
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0303
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   A3MZ21
- ProtClustDB:   PRK01742
- BioCyc:   APLE416269:APL_0303-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- Gene3D:   G3DSA:3.40.50.10070
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 44796; Mature: 44796

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKSRFTSIIGVITLFFSQTVTAESDVVISVDEGVSMAQPIAVVPFKANGGVPADVGQI
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHH
IADDLRNSGKFTPVERSKLPAQPGSAAEVNSQQWTDIGVDSVVVGQVTPTGGGYNVAYQL
HHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHH
VDTLSNPATVLAQGAFNVPAAQIRQGAHTVSDQVFEKITQIRGAFRTKIAYVVQRGVSSY
HHHCCCCCEEEECCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ELRVSDYDGYNAFTVVKSKEPLMSPEWSPDGSRLAYVTFENKKAQVVVHDLRSGSRRVVA
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCEEEE
ALRGHNGAPAFSPDGSRIAFASNQDGELDIYVVGANGGKPSKLTANAGNNTEPSWSPDGS
EEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCC
TIYFTSDRAGSPQVYRMGSSGGGASPMGGSGSYNAKVSSDGKNLIMIAGDKVVKRDLASG
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHCC
GTEVLSSTFLDESPSISPNGIMVIYSSTKGTSKVLQLVSADGRFKANLPGAGGQFKFPAW
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEECCCCCCCEEECCCC
SPYLTK
CCCCCC
>Mature Secondary Structure
MKLKSRFTSIIGVITLFFSQTVTAESDVVISVDEGVSMAQPIAVVPFKANGGVPADVGQI
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHH
IADDLRNSGKFTPVERSKLPAQPGSAAEVNSQQWTDIGVDSVVVGQVTPTGGGYNVAYQL
HHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHH
VDTLSNPATVLAQGAFNVPAAQIRQGAHTVSDQVFEKITQIRGAFRTKIAYVVQRGVSSY
HHHCCCCCEEEECCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ELRVSDYDGYNAFTVVKSKEPLMSPEWSPDGSRLAYVTFENKKAQVVVHDLRSGSRRVVA
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCEEEE
ALRGHNGAPAFSPDGSRIAFASNQDGELDIYVVGANGGKPSKLTANAGNNTEPSWSPDGS
EEECCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCC
TIYFTSDRAGSPQVYRMGSSGGGASPMGGSGSYNAKVSSDGKNLIMIAGDKVVKRDLASG
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHCC
GTEVLSSTFLDESPSISPNGIMVIYSSTKGTSKVLQLVSADGRFKANLPGAGGQFKFPAW
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEECCCCCCCEEECCCC
SPYLTK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA