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The map label for this gene is tolA [C]

Identifier: 126207786

GI number: 126207786

Start: 334285

End: 335580

Strand: Direct

Name: tolA [C]

Synonym: APL_0302

Alternate gene names: 126207786

Gene position: 334285-335580 (Clockwise)

Preceding gene: 126207785

Following gene: 126207787

Centisome position: 14.7

GC content: 43.21

Gene sequence:

>1296_bases
GTGAAATCTAAAAACGACAGACTGGAACTGGCGGTTATAGTTTCAATTCTATTACATGCATTGTTGATCGGATTGCTTAT
ACTCGGTTCATTTTTTACGAAAACTACGTTGGAAGAAGCTGCCGGCGGTAGCGGTGGAACCGGAGAAGAATTTGAAGCAG
TGATGGTTGATACCGGGCAGGTTGCTGCCGAGTATGGGCGTTTAAAATCCGATAAAAAGGGTAATGCTAAACCGAAGGTA
GAGGAAAAAGAAGAGCCGAAACCGGAGGAAGTAGTAGAGGACGAGCCTACACCTCAAGAAATTGCCGAAGAACAGGCGAA
AGAACAACAACGCATTGCAATGATTGAAGAGCAGAAAAAGCAAGAAGAGCAACGCAAAGAAGAACAGCGTAAAAAGGCTG
AAGAAAAACGTCAGCAAGAGATTGAAAAGCAAGAACAACTTAAAAAAGAGCAAGCGGAAGAAGCTACACGTAAGAAAGCT
GCCGAAGCCGCTCGTTTAAAAGCTGAAGCGGAAGCTAAGAATTTAGAAGCTGCGGCAAAAGCGGCGGAAGAAGAGAAAAA
GGCAAAAGAAGCTCAGAAAAAACTTGAACAGCAAAAGAAACTTGAAGAGCAAAAACAAGCTGAGAAAGAAGCGAAATTAA
AAGCTGAAAAAGAGGCTAAAGAAAAGGCTGAAAAAGAAGCTAAAGCTAAGGCTGAGAAAGAGGCTAAAGAAAAAGCGGAA
AAAGAAGCGAAATTAAAAGCTGAAAAAGAGGCTAAAGAAAAGGCTGAAAAAGAAGCGAAACTAAAAGCTGAGAAAGATGC
GAAGGCTAAAGCTGAGAAAGAAGCTAAAGCGAAGGCGGCGGCAGAAGCGAAAGCCAAAGCGGATGCGGCGGCTAAGGCTG
CTCAAGCTAAGAATAACAAAGCGCTTGATGATTTCTTAAGCGGCGGCGATATTGGCGGCGGTTCCAGCAAGGGTGGCAAT
AAAAATACGGCGGGTTCTCAAGGAAACGGTAATGCTAAGGGAATAGGAGACGGAAGAGGTACCGCCGATACGGGATATGC
TCAGTTAATTAAGAAAAAATTAGCGCGAACCTATCGTGTAGATCCGAGTTTCAGCGGGCGTGAATGCCAAGTCAAGATCT
TTATTGATCCGGCAGGTAATATTACTAACCACCAAGTATTATCGGGTCCTGACGACATTTGTCGTGCTGCAGTAACGGCG
ATTACTTCGGCAAGAAATGTTCCGAGAGCGCCAAATGAAGAGACTTATAACAAATATAAATCTCCGATTATTAAATTCGG
TTTAAAAGTTTTATAA

Upstream 100 bases:

>100_bases
TCCTTATGAAGAAGTGATTAAAGGAATCGTACTTTTAAAAGATGCGGGTATTAAGAACGCAGGGTTAATGACCCAGGGCA
AATAGTAGCAAGGATTATGT

Downstream 100 bases:

>100_bases
CAACCAAGAGGTTAAAAATGAAATTAAAATCTCGTTTTACGAGTATTATCGGCGTTATCACCCTCTTTTTCAGCCAAACG
GTAACGGCTGAATCAGATGT

Product: cell envelope integrity inner membrane protein TolA

Products: NA

Alternate protein names: L-Pal System TolA; NB Family Auxiliary Protein TolA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKSKNDRLELAVIVSILLHALLIGLLILGSFFTKTTLEEAAGGSGGTGEEFEAVMVDTGQVAAEYGRLKSDKKGNAKPKV
EEKEEPKPEEVVEDEPTPQEIAEEQAKEQQRIAMIEEQKKQEEQRKEEQRKKAEEKRQQEIEKQEQLKKEQAEEATRKKA
AEAARLKAEAEAKNLEAAAKAAEEEKKAKEAQKKLEQQKKLEEQKQAEKEAKLKAEKEAKEKAEKEAKAKAEKEAKEKAE
KEAKLKAEKEAKEKAEKEAKLKAEKDAKAKAEKEAKAKAAAEAKAKADAAAKAAQAKNNKALDDFLSGGDIGGGSSKGGN
KNTAGSQGNGNAKGIGDGRGTADTGYAQLIKKKLARTYRVDPSFSGRECQVKIFIDPAGNITNHQVLSGPDDICRAAVTA
ITSARNVPRAPNEETYNKYKSPIIKFGLKVL

Sequences:

>Translated_431_residues
MKSKNDRLELAVIVSILLHALLIGLLILGSFFTKTTLEEAAGGSGGTGEEFEAVMVDTGQVAAEYGRLKSDKKGNAKPKV
EEKEEPKPEEVVEDEPTPQEIAEEQAKEQQRIAMIEEQKKQEEQRKEEQRKKAEEKRQQEIEKQEQLKKEQAEEATRKKA
AEAARLKAEAEAKNLEAAAKAAEEEKKAKEAQKKLEQQKKLEEQKQAEKEAKLKAEKEAKEKAEKEAKAKAEKEAKEKAE
KEAKLKAEKEAKEKAEKEAKLKAEKDAKAKAEKEAKAKAAAEAKAKADAAAKAAQAKNNKALDDFLSGGDIGGGSSKGGN
KNTAGSQGNGNAKGIGDGRGTADTGYAQLIKKKLARTYRVDPSFSGRECQVKIFIDPAGNITNHQVLSGPDDICRAAVTA
ITSARNVPRAPNEETYNKYKSPIIKFGLKVL
>Mature_431_residues
MKSKNDRLELAVIVSILLHALLIGLLILGSFFTKTTLEEAAGGSGGTGEEFEAVMVDTGQVAAEYGRLKSDKKGNAKPKV
EEKEEPKPEEVVEDEPTPQEIAEEQAKEQQRIAMIEEQKKQEEQRKEEQRKKAEEKRQQEIEKQEQLKKEQAEEATRKKA
AEAARLKAEAEAKNLEAAAKAAEEEKKAKEAQKKLEQQKKLEEQKQAEKEAKLKAEKEAKEKAEKEAKAKAEKEAKEKAE
KEAKLKAEKEAKEKAEKEAKLKAEKDAKAKAEKEAKAKAAAEAKAKADAAAKAAQAKNNKALDDFLSGGDIGGGSSKGGN
KNTAGSQGNGNAKGIGDGRGTADTGYAQLIKKKLARTYRVDPSFSGRECQVKIFIDPAGNITNHQVLSGPDDICRAAVTA
ITSARNVPRAPNEETYNKYKSPIIKFGLKVL

Specific function: Involved In The Tonb-Independent Uptake Of Group A Colicins (Colicins A, E1, E2, E3, And K). Necessary For The Colicins To Reach Their Respective Targets After Initial Binding To The Bacteria. Also Involved In The Translocation Of Bacteriophage DNA. [C]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Type II Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47015; Mature: 47015

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA