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The map label for this gene is dnaQ

Identifier: 126207670

GI number: 126207670

Start: 200701

End: 201357

Strand: Direct

Name: dnaQ

Synonym: APL_0182

Alternate gene names: 126207670

Gene position: 200701-201357 (Clockwise)

Preceding gene: 126207669

Following gene: 126207676

Centisome position: 8.82

GC content: 45.81

Gene sequence:

>657_bases
ATGACAGAACAAGTAACAGACTATAATTTCTTAAAAAATCGTTTTCGCGGCTATTTACCGGTCATTATTGATGTGGAAAC
CGCCGGATTGAATGCGCAAACCGACGCTTTATTGGAACTGGCGGCAATCACGGTCAAAATGGATGAGAACGGTTATTTGG
TGCCGGATCAAAAATGCCATTTTCATATTCAGCCGTTTGAAGGGGCGAATATCAATCCCGATTCGCTAAAATTTAACGGC
ATTGATATTGATAACCCGTTGCGCGGTGCGGTGCCGGAAAATATTGCGATTCCCGAAATGTTTAAAATGGTACGCCGTGC
AATGAAAGAGCAAGGTTGCCAGCGCGCGGTGATTGTGGCGCATAATGCGACTTTCGATCAGGCGTTCGTACAAGCGGCGG
TAAAGCGTATCAATGCGAAGCGTGATCCGTTCCATCCGTTTGCAATGTTTGATACCGCAACGCTTGCCGGTTTTATGTAC
GGGCAAACCGTCTTAGTCAAAGCCTGCCAAATGGCAAAAATTCCGTTTGACGGTAAACAGGCACATTCCGCTTTATACGA
CACCGAAAAAACCACCGAGTTATTCTGTGCGATGGTGAATCGCTTAAAAGATTTGGGCGGCTTTCCGTTAGTCAGCGATA
GCGAATCAAACGTATAA

Upstream 100 bases:

>100_bases
TGGCGTAAGCGGTCAAATTTTGAGGAAATTTTGCAAATTCTCTTTAAAATCCGACCGCTTTTTCTTTAGACGGATAGAGA
TTGTCCGTAATGAACAGACA

Downstream 100 bases:

>100_bases
TATATTTAAAAAAACAAGCGGTCTATTTTCCTTATTGCTTTGCAAAAAAGTCGGGAAAATAGACCGCTTGTGTATTTCCA
GCTCGTCTAACTAAGACAAC

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTEQVTDYNFLKNRFRGYLPVIIDVETAGLNAQTDALLELAAITVKMDENGYLVPDQKCHFHIQPFEGANINPDSLKFNG
IDIDNPLRGAVPENIAIPEMFKMVRRAMKEQGCQRAVIVAHNATFDQAFVQAAVKRINAKRDPFHPFAMFDTATLAGFMY
GQTVLVKACQMAKIPFDGKQAHSALYDTEKTTELFCAMVNRLKDLGGFPLVSDSESNV

Sequences:

>Translated_218_residues
MTEQVTDYNFLKNRFRGYLPVIIDVETAGLNAQTDALLELAAITVKMDENGYLVPDQKCHFHIQPFEGANINPDSLKFNG
IDIDNPLRGAVPENIAIPEMFKMVRRAMKEQGCQRAVIVAHNATFDQAFVQAAVKRINAKRDPFHPFAMFDTATLAGFMY
GQTVLVKACQMAKIPFDGKQAHSALYDTEKTTELFCAMVNRLKDLGGFPLVSDSESNV
>Mature_217_residues
TEQVTDYNFLKNRFRGYLPVIIDVETAGLNAQTDALLELAAITVKMDENGYLVPDQKCHFHIQPFEGANINPDSLKFNGI
DIDNPLRGAVPENIAIPEMFKMVRRAMKEQGCQRAVIVAHNATFDQAFVQAAVKRINAKRDPFHPFAMFDTATLAGFMYG
QTVLVKACQMAKIPFDGKQAHSALYDTEKTTELFCAMVNRLKDLGGFPLVSDSESNV

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=205, Percent_Identity=62.4390243902439, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_ACTP2 (A3MYQ4)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052895.1
- ProteinModelPortal:   A3MYQ4
- SMR:   A3MYQ4
- STRING:   A3MYQ4
- GeneID:   4848650
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0182
- eggNOG:   COG0847
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- PhylomeDB:   A3MYQ4
- ProtClustDB:   PRK05168
- BioCyc:   APLE416269:APL_0182-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 24294; Mature: 24163

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQVTDYNFLKNRFRGYLPVIIDVETAGLNAQTDALLELAAITVKMDENGYLVPDQKCH
CCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHEEEEEEEEECCCCEECCCCCCE
FHIQPFEGANINPDSLKFNGIDIDNPLRGAVPENIAIPEMFKMVRRAMKEQGCQRAVIVA
EEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
HNATFDQAFVQAAVKRINAKRDPFHPFAMFDTATLAGFMYGQTVLVKACQMAKIPFDGKQ
ECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AHSALYDTEKTTELFCAMVNRLKDLGGFPLVSDSESNV
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
TEQVTDYNFLKNRFRGYLPVIIDVETAGLNAQTDALLELAAITVKMDENGYLVPDQKCH
CCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHEEEEEEEEECCCCEECCCCCCE
FHIQPFEGANINPDSLKFNGIDIDNPLRGAVPENIAIPEMFKMVRRAMKEQGCQRAVIVA
EEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
HNATFDQAFVQAAVKRINAKRDPFHPFAMFDTATLAGFMYGQTVLVKACQMAKIPFDGKQ
ECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AHSALYDTEKTTELFCAMVNRLKDLGGFPLVSDSESNV
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA