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The map label for this gene is gloA [H]

Identifier: 126207669

GI number: 126207669

Start: 200154

End: 200561

Strand: Direct

Name: gloA [H]

Synonym: APL_0181

Alternate gene names: 126207669

Gene position: 200154-200561 (Clockwise)

Preceding gene: 126207665

Following gene: 126207670

Centisome position: 8.8

GC content: 43.63

Gene sequence:

>408_bases
ATGCGAATTTTACATACCATGTTACGCGTGGGCGATTTAGATCGTTCAATTAAATTTTATACCGAAGTATTAGGTATGCG
TTTACTTCGTACCAGCGAAAACCCTCAATATAAATATTCATTAGCATTTGTCGGTTATGCGGACGAAAGTGAAAGTGCGG
TAATCGAATTAACTTATAACTGGGGCGTAGAAAGTTATGAGTTAGGTACGGCATTCGGTCACGTGGCGTTAGGCGTGGAT
GACATTTATGCAACGATCGAAAGCGTACGTGCGGCGGGCGGTAAAATTACCCGTGAGCCGGGTCCGGTATTAGGCGGAAC
AACGGTCATCGCGTTTGCCGAAGATCCGGACGGTTATAAAATCGAGTTTATCGAAAATAAAAATGCACAGGCTGCATTAG
GTAACTAA

Upstream 100 bases:

>100_bases
GTGACATAAATTAAACGGATAAGTTAAATTGGAAAATGATTTTGATGTGGAATTATGCCATAATACACGCAGTTTTTTAA
CTTAAATAAGGAATGAAACT

Downstream 100 bases:

>100_bases
TCGTATTTGCCGAAAAATAGAAATCCCTAACTATTTTTATGGCGTAAGCGGTCAAATTTTGAGGAAATTTTGCAAATTCT
CTTTAAAATCCGACCGCTTT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRILHTMLRVGDLDRSIKFYTEVLGMRLLRTSENPQYKYSLAFVGYADESESAVIELTYNWGVESYELGTAFGHVALGVD
DIYATIESVRAAGGKITREPGPVLGGTTVIAFAEDPDGYKIEFIENKNAQAALGN

Sequences:

>Translated_135_residues
MRILHTMLRVGDLDRSIKFYTEVLGMRLLRTSENPQYKYSLAFVGYADESESAVIELTYNWGVESYELGTAFGHVALGVD
DIYATIESVRAAGGKITREPGPVLGGTTVIAFAEDPDGYKIEFIENKNAQAALGN
>Mature_135_residues
MRILHTMLRVGDLDRSIKFYTEVLGMRLLRTSENPQYKYSLAFVGYADESESAVIELTYNWGVESYELGTAFGHVALGVD
DIYATIESVRAAGGKITREPGPVLGGTTVIAFAEDPDGYKIEFIENKNAQAALGN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=155, Percent_Identity=36.7741935483871, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=73.3333333333333, Blast_Score=209, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.8489208633094, Blast_Score=94, Evalue=7e-21,
Organism=Drosophila melanogaster, GI20129743, Length=142, Percent_Identity=36.6197183098592, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920404, Length=141, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14828; Mature: 14828

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVGDLDRSIKFYTEVLGMRLLRTSENPQYKYSLAFVGYADESESAVIELTYN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEEEEEC
WGVESYELGTAFGHVALGVDDIYATIESVRAAGGKITREPGPVLGGTTVIAFAEDPDGYK
CCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEECCCCEE
IEFIENKNAQAALGN
EEEEECCCCEEECCC
>Mature Secondary Structure
MRILHTMLRVGDLDRSIKFYTEVLGMRLLRTSENPQYKYSLAFVGYADESESAVIELTYN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEEEEEC
WGVESYELGTAFGHVALGVDDIYATIESVRAAGGKITREPGPVLGGTTVIAFAEDPDGYK
CCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEECCCCEE
IEFIENKNAQAALGN
EEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 9719565 [H]