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The map label for this gene is folK [H]

Identifier: 126207665

GI number: 126207665

Start: 195781

End: 196251

Strand: Direct

Name: folK [H]

Synonym: APL_0177

Alternate gene names: 126207665

Gene position: 195781-196251 (Clockwise)

Preceding gene: 126207664

Following gene: 126207669

Centisome position: 8.61

GC content: 42.46

Gene sequence:

>471_bases
ATGAAAACGGTTTATATTGCTTTAGGTTCCAATTTGGATAACCCGCTTGCGCAAGTTAAACAAGCGGTCGAATCTTTAAA
AACATTTGCAATTAATTTTGAAATTAGCCCGTTCTACGGTAGTAAGCCGGTCGGGCCGCAAGATCAGCCGGATTATGTGA
ATGCGGTGGCAAAATTCGATACCGATTTAACGGCGCTGGAATTGCTGGATAAATTACAAAGCATTGAAAACGCCCAAGGA
AGAGTGCGAATTCGCCGTTGGGGCGAACGTACGCTGGATTTGGATATTATCTTATACGGCGATGAACGGATTCACAGCGA
ACGTTTAACCGTACCGCATATTGAAATGCAAAATCGTGAGTTTGTGATTGTGCCTATGTATGATCTTAACCCCGATTTAG
TGCTGCCAAGCGGTCAAAAATTAGCGGAAATTTACCAACGCTTTAAACATCATCAAATGGCCACATTCTAA

Upstream 100 bases:

>100_bases
AAAGCCGGCGAAGAAAGCCTCGGCTGAATAAATTGAAGTATGGCGCAAGTGAGAGCTTGTGCCTTTTCTTTTAGCAAGTT
TGACAGAAACGGATATGATG

Downstream 100 bases:

>100_bases
GTAAAAAATGCACGGCAATCCGTGCATTTTTTGTAACTTATTCTATCGGAGCTTCAACCACCGCTTCCGATTTCGCCGCT
TTATTGTCCGAGGTTTGCAA

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKTVYIALGSNLDNPLAQVKQAVESLKTFAINFEISPFYGSKPVGPQDQPDYVNAVAKFDTDLTALELLDKLQSIENAQG
RVRIRRWGERTLDLDIILYGDERIHSERLTVPHIEMQNREFVIVPMYDLNPDLVLPSGQKLAEIYQRFKHHQMATF

Sequences:

>Translated_156_residues
MKTVYIALGSNLDNPLAQVKQAVESLKTFAINFEISPFYGSKPVGPQDQPDYVNAVAKFDTDLTALELLDKLQSIENAQG
RVRIRRWGERTLDLDIILYGDERIHSERLTVPHIEMQNREFVIVPMYDLNPDLVLPSGQKLAEIYQRFKHHQMATF
>Mature_156_residues
MKTVYIALGSNLDNPLAQVKQAVESLKTFAINFEISPFYGSKPVGPQDQPDYVNAVAKFDTDLTALELLDKLQSIENAQG
RVRIRRWGERTLDLDIILYGDERIHSERLTVPHIEMQNREFVIVPMYDLNPDLVLPSGQKLAEIYQRFKHHQMATF

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=147, Percent_Identity=50.3401360544218, Blast_Score=149, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324073, Length=137, Percent_Identity=32.8467153284672, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17916; Mature: 17916

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVYIALGSNLDNPLAQVKQAVESLKTFAINFEISPFYGSKPVGPQDQPDYVNAVAKFD
CCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
TDLTALELLDKLQSIENAQGRVRIRRWGERTLDLDIILYGDERIHSERLTVPHIEMQNRE
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCEECCCCCCCCCEEECCCC
FVIVPMYDLNPDLVLPSGQKLAEIYQRFKHHQMATF
EEEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTVYIALGSNLDNPLAQVKQAVESLKTFAINFEISPFYGSKPVGPQDQPDYVNAVAKFD
CCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
TDLTALELLDKLQSIENAQGRVRIRRWGERTLDLDIILYGDERIHSERLTVPHIEMQNRE
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCEECCCCCCCCCEEECCCC
FVIVPMYDLNPDLVLPSGQKLAEIYQRFKHHQMATF
EEEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10080886 [H]