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The map label for this gene is pcnB [H]

Identifier: 126207664

GI number: 126207664

Start: 194179

End: 195711

Strand: Direct

Name: pcnB [H]

Synonym: APL_0176

Alternate gene names: 126207664

Gene position: 194179-195711 (Clockwise)

Preceding gene: 126207663

Following gene: 126207665

Centisome position: 8.54

GC content: 43.25

Gene sequence:

>1533_bases
TTGTTTTCTCGACAAAAAAAATCAAAAAAACAACCGCTTACCGAACAAGAAACGCTTAGTGCTAAGCAAGAAAGAAAAGT
TAAAGCGGTAGAATTACAACCTAAATCCAAGCGTCCCGCTCCGGCTTTTAACGAACAGTCGGAACGTTCTACGAAGAAAC
CGAAAAAGCACTCGGATCTTCCTTCCCATTTATTGCACAAAAAATTTACAGTAAATGCCGGACATTATGATATTACGCCG
AAAGATTTCCCTAAAAATGCGTTAGCGATTGTTGAAAAATTACAACGTGCCGGTTTTGAAGCTTATGTGGTCGGCGGCTG
TATTCGAGATTTATTGCTAGGGCATAAGCCGAAAGATTTTGATGTTGCGACCAATGCAAAACCGGAAGAAATTCAAAAAA
TATTCGGTCGTCAATGCCGTTTAATCGGACGTCGTTTTCGTTTGGCACATATCGTATTCGGACGTGATATTTATGAAGTG
GCGACTTTCCGTGCCGATCACGCCAGCCATAATAGCGATAAGATTTCTAAAGTAAGCGAAGAAGGAATGTTGTTGCGCGA
TAATGTGTACGGTACCTTACGCGAAGATGCGCAACGACGTGATTTTAGCGTGAATTCGCTCTATTATGATCCGAAACATA
ACTTGATTTTCGACTTCTTCGACGGTATTGCGGATCTGAAAGCGGGAAAACTGCGTTTAATCGGTGATCCGGTGGTGCGC
TATCAAGAAGATCCGGTACGAATGTTACGCGCGGTTCGTTTTATGGCAAAATTGGATATGTTTTTGGATAAACCGAGCGA
TACGCCGATTCGTGAATTGGCGCATTTATTGAAAAACATTCCGGCGGCGCGTTTATTTGACGAATCGTTGAAATTATTTC
AATCGGGACAAGGTTTTAAAACCTATCAATTAATGCGTCAATACGGCTTATTCGAGCAGTTATTTCCGGTACTTACGCCG
TTTTTTACCGAACGTAACGATTCGAATGCGGAGCGTATGTTAAGTAAAGCGCTGAATTCGACCGATGATCGTATTCGTGA
TAATTTACGGGTGAATCCGGCGTTTTTATTCGCCGCTCTTCTTTGGTATCCGTTACGTGAGAAAATGGAAGTGCTGAAAA
ACGAAGGCGGTTTAAATAGTCATGATGCGATGATGCTGGCGGCGAATGACGTGTTAGCCGAAAGTTGTAAAGCGATTGCA
TTACATCGCCGTCATACCTCTGTGATTCGTGATATTTGGGCGTTACAGTTCCAAATGACCAAACGTTCGGGCAAACGTCC
GCAACAGACCTTAGAACACGTAAAATTCCGTGCGGGTTTCGATTTGTTAGTGATGCGTGCCGAGATTGAAGGCGGCGATT
TAGTCGAGCTTTCCGCTTGGTGGCACGAATATCAATTCAGTAATGACGCACAACGTGCGGAAATGCTAAAAGCGGTCAGA
AATGTGCCGAATTTTGCAGGCGAAGAAAAGAAAAAACGTCGCCGTAAAACATTTAGAAAGAAAAAGCCGGCGAAGAAAGC
CTCGGCTGAATAA

Upstream 100 bases:

>100_bases
TTACATTGTCCATTATTTTTATGATCATTTAGTTAGTGTCGTTTATCAATCGATTAATTTTATTCCCTTGAGGTGGATTA
TTTTTAACTATATTAAGTCG

Downstream 100 bases:

>100_bases
ATTGAAGTATGGCGCAAGTGAGAGCTTGTGCCTTTTCTTTTAGCAAGTTTGACAGAAACGGATATGATGATGAAAACGGT
TTATATTGCTTTAGGTTCCA

Product: putative poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MFSRQKKSKKQPLTEQETLSAKQERKVKAVELQPKSKRPAPAFNEQSERSTKKPKKHSDLPSHLLHKKFTVNAGHYDITP
KDFPKNALAIVEKLQRAGFEAYVVGGCIRDLLLGHKPKDFDVATNAKPEEIQKIFGRQCRLIGRRFRLAHIVFGRDIYEV
ATFRADHASHNSDKISKVSEEGMLLRDNVYGTLREDAQRRDFSVNSLYYDPKHNLIFDFFDGIADLKAGKLRLIGDPVVR
YQEDPVRMLRAVRFMAKLDMFLDKPSDTPIRELAHLLKNIPAARLFDESLKLFQSGQGFKTYQLMRQYGLFEQLFPVLTP
FFTERNDSNAERMLSKALNSTDDRIRDNLRVNPAFLFAALLWYPLREKMEVLKNEGGLNSHDAMMLAANDVLAESCKAIA
LHRRHTSVIRDIWALQFQMTKRSGKRPQQTLEHVKFRAGFDLLVMRAEIEGGDLVELSAWWHEYQFSNDAQRAEMLKAVR
NVPNFAGEEKKKRRRKTFRKKKPAKKASAE

Sequences:

>Translated_510_residues
MFSRQKKSKKQPLTEQETLSAKQERKVKAVELQPKSKRPAPAFNEQSERSTKKPKKHSDLPSHLLHKKFTVNAGHYDITP
KDFPKNALAIVEKLQRAGFEAYVVGGCIRDLLLGHKPKDFDVATNAKPEEIQKIFGRQCRLIGRRFRLAHIVFGRDIYEV
ATFRADHASHNSDKISKVSEEGMLLRDNVYGTLREDAQRRDFSVNSLYYDPKHNLIFDFFDGIADLKAGKLRLIGDPVVR
YQEDPVRMLRAVRFMAKLDMFLDKPSDTPIRELAHLLKNIPAARLFDESLKLFQSGQGFKTYQLMRQYGLFEQLFPVLTP
FFTERNDSNAERMLSKALNSTDDRIRDNLRVNPAFLFAALLWYPLREKMEVLKNEGGLNSHDAMMLAANDVLAESCKAIA
LHRRHTSVIRDIWALQFQMTKRSGKRPQQTLEHVKFRAGFDLLVMRAEIEGGDLVELSAWWHEYQFSNDAQRAEMLKAVR
NVPNFAGEEKKKRRRKTFRKKKPAKKASAE
>Mature_510_residues
MFSRQKKSKKQPLTEQETLSAKQERKVKAVELQPKSKRPAPAFNEQSERSTKKPKKHSDLPSHLLHKKFTVNAGHYDITP
KDFPKNALAIVEKLQRAGFEAYVVGGCIRDLLLGHKPKDFDVATNAKPEEIQKIFGRQCRLIGRRFRLAHIVFGRDIYEV
ATFRADHASHNSDKISKVSEEGMLLRDNVYGTLREDAQRRDFSVNSLYYDPKHNLIFDFFDGIADLKAGKLRLIGDPVVR
YQEDPVRMLRAVRFMAKLDMFLDKPSDTPIRELAHLLKNIPAARLFDESLKLFQSGQGFKTYQLMRQYGLFEQLFPVLTP
FFTERNDSNAERMLSKALNSTDDRIRDNLRVNPAFLFAALLWYPLREKMEVLKNEGGLNSHDAMMLAANDVLAESCKAIA
LHRRHTSVIRDIWALQFQMTKRSGKRPQQTLEHVKFRAGFDLLVMRAEIEGGDLVELSAWWHEYQFSNDAQRAEMLKAVR
NVPNFAGEEKKKRRRKTFRKKKPAKKASAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=221, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI87081691, Length=404, Percent_Identity=53.7128712871287, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1789436, Length=227, Percent_Identity=26.8722466960352, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=217, Percent_Identity=27.1889400921659, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281360112, Length=245, Percent_Identity=26.1224489795918, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357337, Length=245, Percent_Identity=26.1224489795918, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 58930; Mature: 58930

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRQKKSKKQPLTEQETLSAKQERKVKAVELQPKSKRPAPAFNEQSERSTKKPKKHSDL
CCCCCHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCC
PSHLLHKKFTVNAGHYDITPKDFPKNALAIVEKLQRAGFEAYVVGGCIRDLLLGHKPKDF
HHHHHHHHEEECCCCEECCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCC
DVATNAKPEEIQKIFGRQCRLIGRRFRLAHIVFGRDIYEVATFRADHASHNSDKISKVSE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHH
EGMLLRDNVYGTLREDAQRRDFSVNSLYYDPKHNLIFDFFDGIADLKAGKLRLIGDPVVR
CCEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHH
YQEDPVRMLRAVRFMAKLDMFLDKPSDTPIRELAHLLKNIPAARLFDESLKLFQSGQGFK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
TYQLMRQYGLFEQLFPVLTPFFTERNDSNAERMLSKALNSTDDRIRDNLRVNPAFLFAAL
HHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHH
LWYPLREKMEVLKNEGGLNSHDAMMLAANDVLAESCKAIALHRRHTSVIRDIWALQFQMT
HHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRSGKRPQQTLEHVKFRAGFDLLVMRAEIEGGDLVELSAWWHEYQFSNDAQRAEMLKAVR
HHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHH
NVPNFAGEEKKKRRRKTFRKKKPAKKASAE
HCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFSRQKKSKKQPLTEQETLSAKQERKVKAVELQPKSKRPAPAFNEQSERSTKKPKKHSDL
CCCCCHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCC
PSHLLHKKFTVNAGHYDITPKDFPKNALAIVEKLQRAGFEAYVVGGCIRDLLLGHKPKDF
HHHHHHHHEEECCCCEECCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCC
DVATNAKPEEIQKIFGRQCRLIGRRFRLAHIVFGRDIYEVATFRADHASHNSDKISKVSE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHH
EGMLLRDNVYGTLREDAQRRDFSVNSLYYDPKHNLIFDFFDGIADLKAGKLRLIGDPVVR
CCEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCHHHH
YQEDPVRMLRAVRFMAKLDMFLDKPSDTPIRELAHLLKNIPAARLFDESLKLFQSGQGFK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
TYQLMRQYGLFEQLFPVLTPFFTERNDSNAERMLSKALNSTDDRIRDNLRVNPAFLFAAL
HHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHH
LWYPLREKMEVLKNEGGLNSHDAMMLAANDVLAESCKAIALHRRHTSVIRDIWALQFQMT
HHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRSGKRPQQTLEHVKFRAGFDLLVMRAEIEGGDLVELSAWWHEYQFSNDAQRAEMLKAVR
HHCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHH
NVPNFAGEEKKKRRRKTFRKKKPAKKASAE
HCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]