Click here to switch to the map view.

The map label for this gene is dksA [H]

Identifier: 126207663

GI number: 126207663

Start: 193587

End: 194036

Strand: Direct

Name: dksA [H]

Synonym: APL_0175

Alternate gene names: 126207663

Gene position: 193587-194036 (Clockwise)

Preceding gene: 126207662

Following gene: 126207664

Centisome position: 8.51

GC content: 43.33

Gene sequence:

>450_bases
ATGTCTCAAGTGGCAAACACCACTTCATTAGGTTTATTAGCTTTAGCGGGCGTAACACCTTACCAACCGAAGAAAGATGA
AGAATATATGAATGATGCGCAAAAAGAGCATTTTCGTAAAATTCTTCGTGCATGGCATGTGCAAATTATGGACGAAGCTG
AGCGTACAAAAAACCAAATGCAGGAAGAAGTCGCTAATTTCGCCGATCCTGCGGACCGCGCCACTCAGGAAGAAGAATTC
AGTCTTGAATTAAGAAACCGTGACCGTGAGCGTAAATTGCTTAAGAAGATTGAGCAAACATTAAATAGCATTGCCGAAGA
CGAATACGGCTATTGCGAAACTTGCGGTGTTGAAATCGGTTTACGTCGTTTAGAAGCGCGCCCGACCGCGGATATGTGTA
TCGATTGCAAAACACTTGCGGAAATCCGTGAAAAGCAAATGGGCTTATAA

Upstream 100 bases:

>100_bases
GGATTACATTTGCTATTTATACGCCCTTTTAAATTAGCGTAAAAATTTTAGTTTGAATTTACTGAATTTAGGATGAACTC
ATCCAACAAGGAGTGAAATT

Downstream 100 bases:

>100_bases
GCTTAATTAAGAATGGATAAACGGCAATGAATATGGACAATGTTACATTGTCCATTATTTTTATGATCATTTAGTTAGTG
TCGTTTATCAATCGATTAAT

Product: DnaK suppressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSQVANTTSLGLLALAGVTPYQPKKDEEYMNDAQKEHFRKILRAWHVQIMDEAERTKNQMQEEVANFADPADRATQEEEF
SLELRNRDRERKLLKKIEQTLNSIAEDEYGYCETCGVEIGLRRLEARPTADMCIDCKTLAEIREKQMGL

Sequences:

>Translated_149_residues
MSQVANTTSLGLLALAGVTPYQPKKDEEYMNDAQKEHFRKILRAWHVQIMDEAERTKNQMQEEVANFADPADRATQEEEF
SLELRNRDRERKLLKKIEQTLNSIAEDEYGYCETCGVEIGLRRLEARPTADMCIDCKTLAEIREKQMGL
>Mature_148_residues
SQVANTTSLGLLALAGVTPYQPKKDEEYMNDAQKEHFRKILRAWHVQIMDEAERTKNQMQEEVANFADPADRATQEEEFS
LELRNRDRERKLLKKIEQTLNSIAEDEYGYCETCGVEIGLRRLEARPTADMCIDCKTLAEIREKQMGL

Specific function: Dosage-dependent suppressor of a dnaK deletion mutation. It suppressed not only the temperature-sensitive growth but also the filamentous phenotype of the dnaK deletion strain, while the defect of lambda growth is not suppressed [H]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=147, Percent_Identity=69.3877551020408, Blast_Score=218, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR020460
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 17241; Mature: 17110

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVANTTSLGLLALAGVTPYQPKKDEEYMNDAQKEHFRKILRAWHVQIMDEAERTKNQM
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEVANFADPADRATQEEEFSLELRNRDRERKLLKKIEQTLNSIAEDEYGYCETCGVEIG
HHHHHHHCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LRRLEARPTADMCIDCKTLAEIREKQMGL
HHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQVANTTSLGLLALAGVTPYQPKKDEEYMNDAQKEHFRKILRAWHVQIMDEAERTKNQM
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEVANFADPADRATQEEEFSLELRNRDRERKLLKKIEQTLNSIAEDEYGYCETCGVEIG
HHHHHHHCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LRRLEARPTADMCIDCKTLAEIREKQMGL
HHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]