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The map label for this gene is tbpA [H]

Identifier: 126207662

GI number: 126207662

Start: 192415

End: 193380

Strand: Direct

Name: tbpA [H]

Synonym: APL_0174

Alternate gene names: 126207662

Gene position: 192415-193380 (Clockwise)

Preceding gene: 126207660

Following gene: 126207663

Centisome position: 8.46

GC content: 42.13

Gene sequence:

>966_bases
ATGAAACTCGCCAAAATAGCCGCATTATCGGCTTTCGTTACCGGTGTCGCTTATGCGCAACAACCTACTTTAGTCGTTTA
TACTTACGATTCGTTTTCATCGGATTGGGGACCTGCTCCTAAATTAGAACCGTTATTTGAAGCGCAATGCCAATGTGACG
TGAAATTTATGCCGTTTGAAGACGGTATTACCATGCTAAACCGTATTCGTTTGGAAGGAAAAAAAACAAAAGCGGACGTA
ATGTTGGGCATTGATAATTTCACGATGGCGGAAGCGGAAAAAACCGGTTTGTTTGCAATGCACGATTTAAACCTAGCTCC
ATTAAATTGGGAGAATAAAACCTTTTATCCGTATGATTATAGCGAGTATGCGTTTGTTTACGATAAAGAAAAATTAGCGA
ATCCGCCGAAATCGTTAAAAGAATTGGTGGAACGTCAAGATTTGAAAGTGATTTACCAAGACCCTCGTACCAGTACGGTC
GGACGCGGTTTATTATTTTGGCTAAATACGGTATATGGCGAGCAAGCCGAACAAGCATGGCAAACCTTAGCCAAACATAC
GGTAACGGTCGGAAAAGGCTGGTCGGAAACTTATAGTGCCTTTTTAAAAGGGGAGTCGGATTTAGTGTTAAGCTATTCCA
CTTCTCCGCTTTATCATCAATGGCATGATAAAACCGATAAATACGTTGCCGCTCAATTTGACGAAGGGCATTTGGTGCAA
ACGGAAGTCGCCGCCATTTTGAATAGCAGTAAACAAAAAGCATTGGCGAAACAATTTTTAGCTTTCTTACACCAGCCGGA
AGCGCAGAAAGTTATTTCGTATCATAATGTGATGAAACCGGTGGTTGGATCAGTACCGGATTCCGCTTTTGATAAATTGC
CGGCTTATAAGCCGTTAGCCTTTAAAATGCCTGATGCTACGCAGGTAAAAAGCTGGTTGGCGACTTGGACGAAAAGCTGG
CAATAA

Upstream 100 bases:

>100_bases
TATCAGCGTAGGGATTTGAGATACTTTGATTCTATTTTCTTACAGAAAAAGTATATTTCGCTCAAATCCTTCTATATTAT
CCGAATTTAGAAGGACATCT

Downstream 100 bases:

>100_bases
AAGAATTATTCATCTTTTTTCATGGCTAAAGGAATCTTTAGCCATTTTTCTTGTCTGCTAAGCGGTGAAAATTATAAAAA
TTTTTGCAAATTCTAATTGC

Product: thiamine-binding periplasmic protein precursor

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKLAKIAALSAFVTGVAYAQQPTLVVYTYDSFSSDWGPAPKLEPLFEAQCQCDVKFMPFEDGITMLNRIRLEGKKTKADV
MLGIDNFTMAEAEKTGLFAMHDLNLAPLNWENKTFYPYDYSEYAFVYDKEKLANPPKSLKELVERQDLKVIYQDPRTSTV
GRGLLFWLNTVYGEQAEQAWQTLAKHTVTVGKGWSETYSAFLKGESDLVLSYSTSPLYHQWHDKTDKYVAAQFDEGHLVQ
TEVAAILNSSKQKALAKQFLAFLHQPEAQKVISYHNVMKPVVGSVPDSAFDKLPAYKPLAFKMPDATQVKSWLATWTKSW
Q

Sequences:

>Translated_321_residues
MKLAKIAALSAFVTGVAYAQQPTLVVYTYDSFSSDWGPAPKLEPLFEAQCQCDVKFMPFEDGITMLNRIRLEGKKTKADV
MLGIDNFTMAEAEKTGLFAMHDLNLAPLNWENKTFYPYDYSEYAFVYDKEKLANPPKSLKELVERQDLKVIYQDPRTSTV
GRGLLFWLNTVYGEQAEQAWQTLAKHTVTVGKGWSETYSAFLKGESDLVLSYSTSPLYHQWHDKTDKYVAAQFDEGHLVQ
TEVAAILNSSKQKALAKQFLAFLHQPEAQKVISYHNVMKPVVGSVPDSAFDKLPAYKPLAFKMPDATQVKSWLATWTKSW
Q
>Mature_321_residues
MKLAKIAALSAFVTGVAYAQQPTLVVYTYDSFSSDWGPAPKLEPLFEAQCQCDVKFMPFEDGITMLNRIRLEGKKTKADV
MLGIDNFTMAEAEKTGLFAMHDLNLAPLNWENKTFYPYDYSEYAFVYDKEKLANPPKSLKELVERQDLKVIYQDPRTSTV
GRGLLFWLNTVYGEQAEQAWQTLAKHTVTVGKGWSETYSAFLKGESDLVLSYSTSPLYHQWHDKTDKYVAAQFDEGHLVQ
TEVAAILNSSKQKALAKQFLAFLHQPEAQKVISYHNVMKPVVGSVPDSAFDKLPAYKPLAFKMPDATQVKSWLATWTKSW
Q

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=307, Percent_Identity=54.071661237785, Blast_Score=322, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36385; Mature: 36385

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAKIAALSAFVTGVAYAQQPTLVVYTYDSFSSDWGPAPKLEPLFEAQCQCDVKFMPFE
CCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEECC
DGITMLNRIRLEGKKTKADVMLGIDNFTMAEAEKTGLFAMHDLNLAPLNWENKTFYPYDY
CCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCCEECCCCC
SEYAFVYDKEKLANPPKSLKELVERQDLKVIYQDPRTSTVGRGLLFWLNTVYGEQAEQAW
CCEEEEEEHHHHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHH
QTLAKHTVTVGKGWSETYSAFLKGESDLVLSYSTSPLYHQWHDKTDKYVAAQFDEGHLVQ
HHHHHHEEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCEEEEECCCCCCHH
TEVAAILNSSKQKALAKQFLAFLHQPEAQKVISYHNVMKPVVGSVPDSAFDKLPAYKPLA
HHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEE
FKMPDATQVKSWLATWTKSWQ
EECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLAKIAALSAFVTGVAYAQQPTLVVYTYDSFSSDWGPAPKLEPLFEAQCQCDVKFMPFE
CCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEECC
DGITMLNRIRLEGKKTKADVMLGIDNFTMAEAEKTGLFAMHDLNLAPLNWENKTFYPYDY
CCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCCEECCCCC
SEYAFVYDKEKLANPPKSLKELVERQDLKVIYQDPRTSTVGRGLLFWLNTVYGEQAEQAW
CCEEEEEEHHHHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHH
QTLAKHTVTVGKGWSETYSAFLKGESDLVLSYSTSPLYHQWHDKTDKYVAAQFDEGHLVQ
HHHHHHEEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCEEEEECCCCCCHH
TEVAAILNSSKQKALAKQFLAFLHQPEAQKVISYHNVMKPVVGSVPDSAFDKLPAYKPLA
HHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEE
FKMPDATQVKSWLATWTKSWQ
EECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]