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The map label for this gene is pyrG

Identifier: 126207624

GI number: 126207624

Start: 151269

End: 152906

Strand: Direct

Name: pyrG

Synonym: APL_0136

Alternate gene names: 126207624

Gene position: 151269-152906 (Clockwise)

Preceding gene: 126207622

Following gene: 126207625

Centisome position: 6.65

GC content: 44.75

Gene sequence:

>1638_bases
ATGGCAACGAATTATATTTTCGTGACGGGCGGTGTTGTTTCATCTTTAGGCAAAGGTATTGCCGCTGCATCTCTCGCATC
AATTCTCGAAGCTCGTGGTTTAAACGTAACCATTATGAAGCTGGATCCTTATATCAATGTCGATCCGGGTACAATGAGCC
CGACGCAACACGGTGAAGTTTTCGTTACGCAAGACGGTGCGGAAACCGATTTAGACTTAGGTCACTATGAGCGTTTTATT
CGTTCTAAAATGAGCAAAGCGAACAACTTTACCAGCGGTAAAATTTACTCCGAAGTATTACGCAAAGAACGCCGCGGCGA
TTATTTAGGTGCGACTATCCAAGTAATCCCGCACATCACTAACGAAATTAAAGAACGTGTGATTGAAGGCGGTAAAGGTC
GTGATGTGGTGATCGTGGAAGTCGGCGGTACGGTAGGCGATATTGAATCATTACCGTTCTTAGAAGCGTTACGTCAGTTA
GCGGTCGATGTCGGTCGAGAAAAAACCTTATTTATGCACTTAACTTTAGTGCCGTATATTCCGACTGCCGGCGAAGTGAA
AACCAAACCAACGCAACACTCGGTAAAAGAATTACTTTCAATCGGTATTCAACCGGACGTGTTAATTTGCCGTTCGGATC
GTGCGATTCCAAGCAACGAGCGTAAAAAAATCGCCTTATTCTGTAACGTGCCGGAACGTGCGGTAATTTCCTTAAAAGAC
GTGGATTCGATTTACCGTATTCCTGAATTACTCAAATCACAAGGTTTGGATACTTTTGTTTGCGATCGTTTCCGCTTAGA
TTGTCCGGAAGCGGATTTAAGCGAATGGGAACAAGTGCTTTATCGCCAAGCGAACCCGACCGGTGAAGTGACTATCGGTA
TGGTAGGTAAATATGTGGAATTACCGGATGCGTATAAATCGGTAAACGAAGCGTTAAAACACGCCGGTTTAACCAATCGC
TTAACGGTCAATATCAAGTATATTGATTCGCAAGATATCGAAACCAAAGGCGTTGAATTACTCCATGGCTTAGATGCGAT
TTTAGTGCCGGGCGGTTTCGGCTACCGTGGCGTAGAAGGTAAAATCCGTACCGCACAATATGCGCGTGAAAACAAAATCC
CTTATTTAGGTATTTGTTTAGGTATGCAAATCGCACTGATCGAATATGCACGTAACGTAGCAGGTTTAACCCAAGCAAAT
TCATCCGAGTTTGATAAAGATTGTCCGCAACCGGTAGTGGGTTTAATTACCGAATGGCAGGATGAGTCGGGTAATGTGGA
AACACGTTCCGATGAGTCGGATTTAGGCGGCACAATGCGTTTAGGCGCACAACAATGTCACTTAATCGAAGGCACTAAAG
CACGTGAAGTTTATGGTGCGGAAACGATTGTTGAGCGTCACCGTCACCGTTATGAAGTAAACAATACTTTATTACCGCAA
ATCGAAGCGGCAGGCTTAAAAGTGAGCGGTTTATCGGCAGACCGTAAATTAGTGGAAATTATTGAAATTCCAAACCACCC
TTGGTTTATTGCGGCGCAGTTCCACCCGGAATTTACTTCGACACCGCGTGACGGTCATCCGTTATTTGCCGGCTTTGTGG
CGGCGGCAAAAGCATATCAAGATAGCCATAAAGCGTAA

Upstream 100 bases:

>100_bases
ATACGTTTTACTATATACTAGGCTCCCGTCCTGATAGTTTTTATTTACTATCCATTCCGAATTTCTTATCTATTTAACTG
ATTAATCAAAGGCAGACAAA

Downstream 100 bases:

>100_bases
TCAACTATTGCTATAATGTGAGGCGAGTTTTATACTCGCCTTATTTTTTACAAAAAATAAACAAAAATTTAACCGCTTAA
TTGTATTAATAAGGAGAAAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MATNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTQDGAETDLDLGHYERFI
RSKMSKANNFTSGKIYSEVLRKERRGDYLGATIQVIPHITNEIKERVIEGGKGRDVVIVEVGGTVGDIESLPFLEALRQL
AVDVGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPSNERKKIALFCNVPERAVISLKD
VDSIYRIPELLKSQGLDTFVCDRFRLDCPEADLSEWEQVLYRQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTNR
LTVNIKYIDSQDIETKGVELLHGLDAILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTQAN
SSEFDKDCPQPVVGLITEWQDESGNVETRSDESDLGGTMRLGAQQCHLIEGTKAREVYGAETIVERHRHRYEVNNTLLPQ
IEAAGLKVSGLSADRKLVEIIEIPNHPWFIAAQFHPEFTSTPRDGHPLFAGFVAAAKAYQDSHKA

Sequences:

>Translated_545_residues
MATNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTQDGAETDLDLGHYERFI
RSKMSKANNFTSGKIYSEVLRKERRGDYLGATIQVIPHITNEIKERVIEGGKGRDVVIVEVGGTVGDIESLPFLEALRQL
AVDVGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPSNERKKIALFCNVPERAVISLKD
VDSIYRIPELLKSQGLDTFVCDRFRLDCPEADLSEWEQVLYRQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTNR
LTVNIKYIDSQDIETKGVELLHGLDAILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTQAN
SSEFDKDCPQPVVGLITEWQDESGNVETRSDESDLGGTMRLGAQQCHLIEGTKAREVYGAETIVERHRHRYEVNNTLLPQ
IEAAGLKVSGLSADRKLVEIIEIPNHPWFIAAQFHPEFTSTPRDGHPLFAGFVAAAKAYQDSHKA
>Mature_544_residues
ATNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTQDGAETDLDLGHYERFIR
SKMSKANNFTSGKIYSEVLRKERRGDYLGATIQVIPHITNEIKERVIEGGKGRDVVIVEVGGTVGDIESLPFLEALRQLA
VDVGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPSNERKKIALFCNVPERAVISLKDV
DSIYRIPELLKSQGLDTFVCDRFRLDCPEADLSEWEQVLYRQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTNRL
TVNIKYIDSQDIETKGVELLHGLDAILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTQANS
SEFDKDCPQPVVGLITEWQDESGNVETRSDESDLGGTMRLGAQQCHLIEGTKAREVYGAETIVERHRHRYEVNNTLLPQI
EAAGLKVSGLSADRKLVEIIEIPNHPWFIAAQFHPEFTSTPRDGHPLFAGFVAAAKAYQDSHKA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.165170556553, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=556, Percent_Identity=44.9640287769784, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI28559083, Length=556, Percent_Identity=44.9640287769784, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI221316689, Length=556, Percent_Identity=44.9640287769784, Blast_Score=471, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=540, Percent_Identity=80.1851851851852, Blast_Score=905, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=615, Percent_Identity=38.3739837398374, Blast_Score=409, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=42.8822495606327, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=41.4762741652021, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=556, Percent_Identity=43.705035971223, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=42.0318725099602, Blast_Score=390, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_ACTP2 (A3MYK8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001052849.1
- ProteinModelPortal:   A3MYK8
- SMR:   A3MYK8
- STRING:   A3MYK8
- GeneID:   4848586
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0136
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   A3MYK8
- ProtClustDB:   PRK05380
- BioCyc:   APLE416269:APL_0136-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60070; Mature: 59939

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 379-379 ACT_SITE 515-515 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTQDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NEIKERVIEGGKGRDVVIVEVGGTVGDIESLPFLEALRQLAVDVGREKTLFMHLTLVPYI
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCC
PTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPSNERKKIALFCNVPERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHEEEHHH
VDSIYRIPELLKSQGLDTFVCDRFRLDCPEADLSEWEQVLYRQANPTGEVTIGMVGKYVE
HHHHHHHHHHHHHCCCCCEEHHCEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
LPDAYKSVNEALKHAGLTNRLTVNIKYIDSQDIETKGVELLHGLDAILVPGGFGYRGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCCCCCC
KIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTQANSSEFDKDCPQPVVGLITEWQ
CCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
DESGNVETRSDESDLGGTMRLGAQQCHLIEGTKAREVYGAETIVERHRHRYEVNNTLLPQ
CCCCCEECCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCC
IEAAGLKVSGLSADRKLVEIIEIPNHPWFIAAQFHPEFTSTPRDGHPLFAGFVAAAKAYQ
CHHCCEEEECCCCCHHEEEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
DSHKA
HCCCC
>Mature Secondary Structure 
ATNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTQDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NEIKERVIEGGKGRDVVIVEVGGTVGDIESLPFLEALRQLAVDVGREKTLFMHLTLVPYI
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCC
PTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPSNERKKIALFCNVPERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHEEEHHH
VDSIYRIPELLKSQGLDTFVCDRFRLDCPEADLSEWEQVLYRQANPTGEVTIGMVGKYVE
HHHHHHHHHHHHHCCCCCEEHHCEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEC
LPDAYKSVNEALKHAGLTNRLTVNIKYIDSQDIETKGVELLHGLDAILVPGGFGYRGVEG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCCCCCC
KIRTAQYARENKIPYLGICLGMQIALIEYARNVAGLTQANSSEFDKDCPQPVVGLITEWQ
CCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
DESGNVETRSDESDLGGTMRLGAQQCHLIEGTKAREVYGAETIVERHRHRYEVNNTLLPQ
CCCCCEECCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCC
IEAAGLKVSGLSADRKLVEIIEIPNHPWFIAAQFHPEFTSTPRDGHPLFAGFVAAAKAYQ
CHHCCEEEECCCCCHHEEEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
DSHKA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA