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The map label for this gene is cysB [H]

Identifier: 126207621

GI number: 126207621

Start: 148207

End: 149190

Strand: Direct

Name: cysB [H]

Synonym: APL_0133

Alternate gene names: 126207621

Gene position: 148207-149190 (Clockwise)

Preceding gene: 126207620

Following gene: 126207622

Centisome position: 6.52

GC content: 42.68

Gene sequence:

>984_bases
ATGAAATTACAGCAATTACGCTACATCGTTGAAATTGTAAATCAAAATCTGAATATTACCGAAGCGGCGGAAACACTTTA
CACTTCTCAACCGGGCATTAGTAAGCAGGTACGTCTATTGGAAAGCGAACTCGGAATTAATATTTTCGAACGAAACGGTA
AGCATATTAAAGGATTAACGCCGGCGGGAGAAAAAATTGTCGCGATTGCGCGCGAATTACTGGTAAAAGCGCAGAGTATT
AAATCGGTGGCGGAAGAACAGACTCGTCCGAATCGAGGCGTCTTACGCATTGCAACGCCGAATACGCAAGCGCGCTATGT
TTTGCCGCCGATTATTGAGAAATTCAAAGCGAAGTATCCGGAGGTCAGCCTACAATTACATCAAGGTTCGCCAAGTCAAA
TTTACGATGCCTTAATGGCGGGCGAAGTGGATCTGGCGATTACGACCGAAGCGCAGCATTTATTTGAAGATGCGATCTTA
TTGCCGTGTTATTTATGGAATCGTTCCGTCATTGTGAAGCCGGATCATCCGTTAGTAAAATTTGTGAACCAAAGCGAGAA
TTTAACGGTCGAAGAACTCGGTAAATATGATTTGATTACTTATACTTTCGGCTTTACCGGACAATCCGATCTCGACCATG
CGTTTAATAAAGAAGGTATCTTACCGCATATCGTGTTTACTGCGACGGATGCCGACGTCATTAAAACTTACGTGCGTTTA
GGCTTAGGGGTCGGGATTATGGCGTCAATGGCGCATACGCCCGCGGACAGTGATTTGGTGGCGATTGATGCAAGTCATTT
ATTCCAATCGAGTATGACGCAAATCGCATTTAAACGCGGCACTTTCTTACGTAATTATATGTATGATTTTATTAACTATT
TCTCGCCGCATTTAACAAAGATGCAAGTAGAGAAAGCGGAACAATTAAGAGAGAGCAATGCTATTCTTCGTTTGTTCGAA
AGCACTAAATTAGAGGAAAAATAG

Upstream 100 bases:

>100_bases
GGTTTAATCATTCGAAAAGTAAGAATGAAACACAGATTCGCCCGACTTATGAAATTAGCTCAATGGATCAGCTTATTGAG
ATTGTTAAAGGAACCGAATA

Downstream 100 bases:

>100_bases
ATGGGATTTATTAAAAAAATCGCACTTTCGTTTGTTGCAATTTTTGCATTAATTTCAACCGCTTGGGCGGATGATTTTAA
TATAAAATATAGTTCTAATT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKLQQLRYIVEIVNQNLNITEAAETLYTSQPGISKQVRLLESELGINIFERNGKHIKGLTPAGEKIVAIARELLVKAQSI
KSVAEEQTRPNRGVLRIATPNTQARYVLPPIIEKFKAKYPEVSLQLHQGSPSQIYDALMAGEVDLAITTEAQHLFEDAIL
LPCYLWNRSVIVKPDHPLVKFVNQSENLTVEELGKYDLITYTFGFTGQSDLDHAFNKEGILPHIVFTATDADVIKTYVRL
GLGVGIMASMAHTPADSDLVAIDASHLFQSSMTQIAFKRGTFLRNYMYDFINYFSPHLTKMQVEKAEQLRESNAILRLFE
STKLEEK

Sequences:

>Translated_327_residues
MKLQQLRYIVEIVNQNLNITEAAETLYTSQPGISKQVRLLESELGINIFERNGKHIKGLTPAGEKIVAIARELLVKAQSI
KSVAEEQTRPNRGVLRIATPNTQARYVLPPIIEKFKAKYPEVSLQLHQGSPSQIYDALMAGEVDLAITTEAQHLFEDAIL
LPCYLWNRSVIVKPDHPLVKFVNQSENLTVEELGKYDLITYTFGFTGQSDLDHAFNKEGILPHIVFTATDADVIKTYVRL
GLGVGIMASMAHTPADSDLVAIDASHLFQSSMTQIAFKRGTFLRNYMYDFINYFSPHLTKMQVEKAEQLRESNAILRLFE
STKLEEK
>Mature_327_residues
MKLQQLRYIVEIVNQNLNITEAAETLYTSQPGISKQVRLLESELGINIFERNGKHIKGLTPAGEKIVAIARELLVKAQSI
KSVAEEQTRPNRGVLRIATPNTQARYVLPPIIEKFKAKYPEVSLQLHQGSPSQIYDALMAGEVDLAITTEAQHLFEDAIL
LPCYLWNRSVIVKPDHPLVKFVNQSENLTVEELGKYDLITYTFGFTGQSDLDHAFNKEGILPHIVFTATDADVIKTYVRL
GLGVGIMASMAHTPADSDLVAIDASHLFQSSMTQIAFKRGTFLRNYMYDFINYFSPHLTKMQVEKAEQLRESNAILRLFE
STKLEEK

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=328, Percent_Identity=61.280487804878, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1788296, Length=314, Percent_Identity=40.4458598726115, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=31.140350877193, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790399, Length=266, Percent_Identity=27.4436090225564, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=301, Percent_Identity=25.9136212624585, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=223, Percent_Identity=26.9058295964126, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790262, Length=210, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=184, Percent_Identity=27.7173913043478, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=251, Percent_Identity=25.4980079681275, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=153, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=177, Percent_Identity=24.2937853107345, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789204, Length=234, Percent_Identity=26.0683760683761, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36853; Mature: 36853

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIVEIVNQNLNITEAAETLYTSQPGISKQVRLLESELGINIFERNGKHIKGLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCEEECCC
PAGEKIVAIARELLVKAQSIKSVAEEQTRPNRGVLRIATPNTQARYVLPPIIEKFKAKYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECHHHHHHHHCCCC
EVSLQLHQGSPSQIYDALMAGEVDLAITTEAQHLFEDAILLPCYLWNRSVIVKPDHPLVK
CEEEEEECCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEEECCCEEEECCCCHHHH
FVNQSENLTVEELGKYDLITYTFGFTGQSDLDHAFNKEGILPHIVFTATDADVIKTYVRL
HHCCCCCCCHHHCCCEEEEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHH
GLGVGIMASMAHTPADSDLVAIDASHLFQSSMTQIAFKRGTFLRNYMYDFINYFSPHLTK
HCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MQVEKAEQLRESNAILRLFESTKLEEK
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLQQLRYIVEIVNQNLNITEAAETLYTSQPGISKQVRLLESELGINIFERNGKHIKGLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCEEECCC
PAGEKIVAIARELLVKAQSIKSVAEEQTRPNRGVLRIATPNTQARYVLPPIIEKFKAKYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECHHHHHHHHCCCC
EVSLQLHQGSPSQIYDALMAGEVDLAITTEAQHLFEDAILLPCYLWNRSVIVKPDHPLVK
CEEEEEECCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEEECCCEEEECCCCHHHH
FVNQSENLTVEELGKYDLITYTFGFTGQSDLDHAFNKEGILPHIVFTATDADVIKTYVRL
HHCCCCCCCHHHCCCEEEEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHH
GLGVGIMASMAHTPADSDLVAIDASHLFQSSMTQIAFKRGTFLRNYMYDFINYFSPHLTK
HCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MQVEKAEQLRESNAILRLFESTKLEEK
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]