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The map label for this gene is pleD [H]

Identifier: 126173611

GI number: 126173611

Start: 1574737

End: 1576293

Strand: Reverse

Name: pleD [H]

Synonym: Sbal_1373

Alternate gene names: 126173611

Gene position: 1576293-1574737 (Counterclockwise)

Preceding gene: 126173612

Following gene: 126173610

Centisome position: 30.74

GC content: 44.51

Gene sequence:

>1557_bases
ATGAAGGATTCGAGCGCTACCGTTTCACAACTTGCCTTGCTGCAACAGAAACTTCATTCGGCCAAACTTGCCCTCGATGA
AGTCAATGAAGACAGAAACGCAAAGTTACAGACATTACTGCAATTTATTGGTCACTTAAGCCTCGCCTGTAAAGGGCAAA
ATCTTGAGCTAGATAATAAACTCGCCAAACTTCGCCATAACTTAAATACGTTTGAGCGAGTCGATGAGGCATTGCCAGAA
TTAGTCGATGTCGAGCGCCTACTAAAAGGCCAATATCACCATGTGATGGTACAACTCGAAGAGAGTCGTACGGGTTTGTC
ACAGGTGATCCGTCAGATCCAACGCGTGAACTCTGCACCTGAAAAACTCAAAAAAGAAGTCAATTACTTCAAACAAGATC
TAGCTAAACCTTTCCACACGGTTTGGGAATATATTCCTAAAGTTAAGCAAATCATCGGCTTCTACGAAACCATATTACAG
CTACAATTTAACGAATCTGAAAAACTCGATGTCTTGCCAAAACACAGGCAGCTAGCCCATGAGCTGGCGCAGATGATTTC
GGAAATCGAGTTTCGTAAAGATCAGCGAGACCAAGTCTTAGTGATCAAAGAAGTGCTCTCTTCTGAGATTGAAGTTGATA
GCTTGCTCGAAGCCTACCAAACCATTTTAGCGTTATTACTTGATAACATCGCCCGCGAGAAATCGGCATCGCAAGAGTTT
TTGTTTGCTCTAAACGATGCCTTAGCCGCAGTGCGTGAAGTGGTCAGTGAATCTTACAACCACAATCAACGCAGTTTTCA
ACTCAAGACCCAGTTGAATCGTGAGATCAACACCCGCGTCGATAACGCCGGTGAAATCATTATCGATGTGGAAGATGTCG
TCGATCTTAAAGCCCAATTGACTGAGCAACTTAACTCCATTCGTTCGGCCTTGGCCCGTAAAGAAGCCTTGGAGCAAAGA
GAGCAAGCCTTACTGCGTAAATCGATGGAGACGATGCGCAAAGAGCTTAATGAATTAAGTAACGAGGCAAACACCTACAA
AGAGCGTTTGTTCGAACAGCAAAAACTTAACTTGCTCGACAGCTTAACTCAATTGCCTAACCGTGCCGCGTTAGAAGAGC
GCATGGAGCTTGAATACCGTAACTATCAACGGCATAAATCTCCCCTGTGGGTGGCGGTTGCCGATATCGACCACTTCAAA
ACTATCAACGACAGCTTTGGTCACAGCACGGGCGACAAAACCCTGCAGGTGATTGCGATGGCATTGAAAAACTCGCTACG
CGATACAGAGTTTGTTGCCCGTTATGGTGGCGAAGAATTTGTCCTGCTATTACCCGATGTAGCACCTGCTGATATCGCTC
AACTTTTAAACAGAGTGCGCGAGAAAGTAAAAAATATTCCATTTAAATTTAAAAATCAGAGAATTACAGTTACAGTATCT
ATAGGGGCTGCGCAAGTTATGGGAAGTGAGCTCATACATGAGACCTTCGAACGCGCCGATGCAGCACTCTACAAAGCGAA
ACATGAAAGCAGAGACAGGGTTGTGATTGACGTATAA

Upstream 100 bases:

>100_bases
ACGCCCAGTGTTATAACATTAGTATCATATGAGTTAACAGCTAATCTGGACATAGGATCGTCATGCTTTTTAGCCTTGCA
GAGTTAGGACAACACTCAGA

Downstream 100 bases:

>100_bases
TTGAGCCCCACTATACGCCTCCTCTAATCACTCCTGTCATGACGAAGGAGTGATAAATGATAGTAAAAGTCAGCCCTAAA
GGCAGCATGGATCAGCTTTC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAPEKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQ
LQFNESEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKEVLSSEIEVDSLLEAYQTILALLLDNIAREKSASQEF
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQLTEQLNSIRSALARKEALEQR
EQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMELEYRNYQRHKSPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV

Sequences:

>Translated_518_residues
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAPEKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQ
LQFNESEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKEVLSSEIEVDSLLEAYQTILALLLDNIAREKSASQEF
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQLTEQLNSIRSALARKEALEQR
EQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMELEYRNYQRHKSPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV
>Mature_518_residues
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNKLAKLRHNLNTFERVDEALPE
LVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAPEKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQ
LQFNESEKLDVLPKHRQLAHELAQMISEIEFRKDQRDQVLVIKEVLSSEIEVDSLLEAYQTILALLLDNIAREKSASQEF
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQLTEQLNSIRSALARKEALEQR
EQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLDSLTQLPNRAALEERMELEYRNYQRHKSPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.2670807453416, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=36.25, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=35.4430379746835, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=32.9113924050633, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=36.25, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87081881, Length=122, Percent_Identity=45.0819672131148, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=33.9181286549708, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=188, Percent_Identity=32.4468085106383, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=29.746835443038, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=28.4023668639053, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=33.9622641509434, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=27.3809523809524, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59623; Mature: 59623

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKLRHNLNTFERVDEALPELVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAP
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHCHHHHHHHHHHHHCCCH
EKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQLQFNESEKLDVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ELAQMISEIEFRKDQRDQVLVIKEVLSSEIEVDSLLEAYQTILALLLDNIAREKSASQEF
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
TEQLNSIRSALARKEALEQREQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTQLPNRAALEERMELEYRNYQRHKSPLWVAVADIDHFKTINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCHHCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MKDSSATVSQLALLQQKLHSAKLALDEVNEDRNAKLQTLLQFIGHLSLACKGQNLELDNK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKLRHNLNTFERVDEALPELVDVERLLKGQYHHVMVQLEESRTGLSQVIRQIQRVNSAP
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHCHHHHHHHHHHHHCCCH
EKLKKEVNYFKQDLAKPFHTVWEYIPKVKQIIGFYETILQLQFNESEKLDVLPKHRQLAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ELAQMISEIEFRKDQRDQVLVIKEVLSSEIEVDSLLEAYQTILALLLDNIAREKSASQEF
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LFALNDALAAVREVVSESYNHNQRSFQLKTQLNREINTRVDNAGEIIIDVEDVVDLKAQL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
TEQLNSIRSALARKEALEQREQALLRKSMETMRKELNELSNEANTYKERLFEQQKLNLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTQLPNRAALEERMELEYRNYQRHKSPLWVAVADIDHFKTINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCHHCCCCCCHHHHHHHHH
ALKNSLRDTEFVARYGGEEFVLLLPDVAPADIAQLLNRVREKVKNIPFKFKNQRITVTVS
HHHHHCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
IGAAQVMGSELIHETFERADAALYKAKHESRDRVVIDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA