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The map label for this gene is ilvD [H]

Identifier: 124485438

GI number: 124485438

Start: 586563

End: 588206

Strand: Reverse

Name: ilvD [H]

Synonym: Mlab_0613

Alternate gene names: 124485438

Gene position: 588206-586563 (Counterclockwise)

Preceding gene: 124485439

Following gene: 124485437

Centisome position: 32.59

GC content: 54.01

Gene sequence:

>1644_bases
ATGCGCAGCGATGATGTGAAATCAGGATACACCAGAGCACCCAACAGGGCTCTTATACGCTCACTCGGGATTTCTGATCA
AGAGATGAAAAAACCCTTTGTCGGGATCGCAAACTCCTGGAACACGATAGTTCCGGGACACACGCATCTCAGAATGGTTG
CCGAACGCGTAAGAGAGGGAGTTGCGGCAGGCGGGGGTGTTGCTTTTGAGTTCAACACGATAGGAATTTGCGACGGTATC
GCGATGGGACATGAAGGGATGCGGTACTCGCTAGCCTCACGGGAAAACATCGCGGATTCAGTTGAACTCATGATCCAGGC
CCACCGGTTCGATGCTCTTGTCTGTATCTGCACTTGTGATAAGATCGTTCCCGGCATGCTTATGGCGGCGGCACGATGTA
ATATTCCGGCGATCGTCGTCACCGGCGGGAACATGCTCCCCGGACATCACAAAGGTTGCGAACTTTCCCTGACCGATATT
TTCGAGGGGGTCGGCAAGGTGGCTGCCCGCAAAATGAGCGAGGAGGAACTTCATGAACTGGAAGCCGCGGCAATGCCTGG
GTGCGGCAGTTGTCAGGGATTATATACGGCAAATACGATGGCCTGCATGACCGAAGCGATGGGAATGTCCCTTCCCGGCT
GTGCGGCGATTCCCGCAGTGGATGCGGCCAAACTCCGGATCGCCTATGAAAGCGGTGTCAGAATCGTTGAGATGATCAAA
GAGGATGTCAAACCCCGTGACATCATCACGAAAAATTCGCTGAAAAACGCCATCGCCGTAGATATGGCGCTTGGCGGTTC
AACGAACACGGTCCTTCACTTAATGGCGATCGCTCAGGAAGCGGATGTCTCGCTGAGCCTTGATGATTTTACCCAGATGG
GAGCAACCGTTCCGCACATCTGTTCGATGCAGCCCGGAGGACCACACTCAATGCAGACGTTATATCACTCAGGGGGGATC
CCCGCGGTCTTCAAACAGATCAGACCGCATCTTGACGACTGTATGACGGTTTCGGGAAAAACAGTGTTTTCGATCGCAGA
CGGCGTGAAATACGTGGATGAAAATGTGATCCACAGTCTGGAAAATGCCGTTCACAAAGCAGGCGGGCTGAAGATCCTGA
AAGGCTCGCTTGCGCCGAACGGGTCGGTGATCAAATCGGCAGCAGTAATCGATGCGATGTGGAAGCATCAGGGACCCGCA
CGGGTTTTTGACGGAGAGAACGAGGCGATGGACGCGATTCTTACCGGAAAGATCGTCGAAGGCGACGTGATCGTTATCCG
GTATGAGGGACCGAAAGGAGGACCGGGCATGCCGGAGATGCTTTCCCCGACCTCGGCACTGATGGGTCTTGGCTATACGC
ATGTAGCTCTTGTGACGGACGGACGTTTTTCCGGCGGAACTCGTGGTCCGTGTATAGGTCATGTTGCTCCGGAAGCAAAG
GCAGGAGGACCGATCGGCCTTGTTCGTGAGGGAGACATCATCAAGATCGATCTCTATGAACAAACACTGGATCTTCTCGT
TGACGAGGCGGAACTTGCAGAGAGAAGAAAGACGTGGGTGCCCAAAGAAAGAGAGTTAAAGGGCGTGCTTGCACGATATG
CACGATACGCTGGTCAGGCCGATAAGGGCGCCGTGCCTGAATAA

Upstream 100 bases:

>100_bases
GCCCGCTTTCCTTTTTTGCGCATTAGCTTTCCTTTAAGTAATCAGAAATTTTCGGCCGCTTGTTTTATTATTGCCTATTA
CCTTTATCTATGTGATTCAG

Downstream 100 bases:

>100_bases
TTTTTTTATTTTTTTTAGAAAGCTCCACTTAAATCGGACGCGTGTGCATCTTCGCTTCAAGGAACCGAAGCCATATTTTT
GCCGTCTGAATGCATTGCCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MRSDDVKSGYTRAPNRALIRSLGISDQEMKKPFVGIANSWNTIVPGHTHLRMVAERVREGVAAGGGVAFEFNTIGICDGI
AMGHEGMRYSLASRENIADSVELMIQAHRFDALVCICTCDKIVPGMLMAAARCNIPAIVVTGGNMLPGHHKGCELSLTDI
FEGVGKVAARKMSEEELHELEAAAMPGCGSCQGLYTANTMACMTEAMGMSLPGCAAIPAVDAAKLRIAYESGVRIVEMIK
EDVKPRDIITKNSLKNAIAVDMALGGSTNTVLHLMAIAQEADVSLSLDDFTQMGATVPHICSMQPGGPHSMQTLYHSGGI
PAVFKQIRPHLDDCMTVSGKTVFSIADGVKYVDENVIHSLENAVHKAGGLKILKGSLAPNGSVIKSAAVIDAMWKHQGPA
RVFDGENEAMDAILTGKIVEGDVIVIRYEGPKGGPGMPEMLSPTSALMGLGYTHVALVTDGRFSGGTRGPCIGHVAPEAK
AGGPIGLVREGDIIKIDLYEQTLDLLVDEAELAERRKTWVPKERELKGVLARYARYAGQADKGAVPE

Sequences:

>Translated_547_residues
MRSDDVKSGYTRAPNRALIRSLGISDQEMKKPFVGIANSWNTIVPGHTHLRMVAERVREGVAAGGGVAFEFNTIGICDGI
AMGHEGMRYSLASRENIADSVELMIQAHRFDALVCICTCDKIVPGMLMAAARCNIPAIVVTGGNMLPGHHKGCELSLTDI
FEGVGKVAARKMSEEELHELEAAAMPGCGSCQGLYTANTMACMTEAMGMSLPGCAAIPAVDAAKLRIAYESGVRIVEMIK
EDVKPRDIITKNSLKNAIAVDMALGGSTNTVLHLMAIAQEADVSLSLDDFTQMGATVPHICSMQPGGPHSMQTLYHSGGI
PAVFKQIRPHLDDCMTVSGKTVFSIADGVKYVDENVIHSLENAVHKAGGLKILKGSLAPNGSVIKSAAVIDAMWKHQGPA
RVFDGENEAMDAILTGKIVEGDVIVIRYEGPKGGPGMPEMLSPTSALMGLGYTHVALVTDGRFSGGTRGPCIGHVAPEAK
AGGPIGLVREGDIIKIDLYEQTLDLLVDEAELAERRKTWVPKERELKGVLARYARYAGQADKGAVPE
>Mature_547_residues
MRSDDVKSGYTRAPNRALIRSLGISDQEMKKPFVGIANSWNTIVPGHTHLRMVAERVREGVAAGGGVAFEFNTIGICDGI
AMGHEGMRYSLASRENIADSVELMIQAHRFDALVCICTCDKIVPGMLMAAARCNIPAIVVTGGNMLPGHHKGCELSLTDI
FEGVGKVAARKMSEEELHELEAAAMPGCGSCQGLYTANTMACMTEAMGMSLPGCAAIPAVDAAKLRIAYESGVRIVEMIK
EDVKPRDIITKNSLKNAIAVDMALGGSTNTVLHLMAIAQEADVSLSLDDFTQMGATVPHICSMQPGGPHSMQTLYHSGGI
PAVFKQIRPHLDDCMTVSGKTVFSIADGVKYVDENVIHSLENAVHKAGGLKILKGSLAPNGSVIKSAAVIDAMWKHQGPA
RVFDGENEAMDAILTGKIVEGDVIVIRYEGPKGGPGMPEMLSPTSALMGLGYTHVALVTDGRFSGGTRGPCIGHVAPEAK
AGGPIGLVREGDIIKIDLYEQTLDLLVDEAELAERRKTWVPKERELKGVLARYARYAGQADKGAVPE

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=605, Percent_Identity=44.1322314049587, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786464, Length=529, Percent_Identity=34.9716446124764, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1788157, Length=519, Percent_Identity=32.1772639691715, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI2367371, Length=531, Percent_Identity=33.1450094161959, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322476, Length=546, Percent_Identity=42.4908424908425, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 58154; Mature: 58154

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00622 HTH_LUXR_1 ; PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDDVKSGYTRAPNRALIRSLGISDQEMKKPFVGIANSWNTIVPGHTHLRMVAERVREG
CCCCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
VAAGGGVAFEFNTIGICDGIAMGHEGMRYSLASRENIADSVELMIQAHRFDALVCICTCD
HCCCCCEEEEEECEEEECCHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEHH
KIVPGMLMAAARCNIPAIVVTGGNMLPGHHKGCELSLTDIFEGVGKVAARKMSEEELHEL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHH
EAAAMPGCGSCQGLYTANTMACMTEAMGMSLPGCAAIPAVDAAKLRIAYESGVRIVEMIK
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHCCCHHHHHHH
EDVKPRDIITKNSLKNAIAVDMALGGSTNTVLHLMAIAQEADVSLSLDDFTQMGATVPHI
HCCCCHHHEEHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCHH
CSMQPGGPHSMQTLYHSGGIPAVFKQIRPHLDDCMTVSGKTVFSIADGVKYVDENVIHSL
HCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHH
ENAVHKAGGLKILKGSLAPNGSVIKSAAVIDAMWKHQGPARVFDGENEAMDAILTGKIVE
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCEEEE
GDVIVIRYEGPKGGPGMPEMLSPTSALMGLGYTHVALVTDGRFSGGTRGPCIGHVAPEAK
CCEEEEEECCCCCCCCCCHHCCHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCCCC
AGGPIGLVREGDIIKIDLYEQTLDLLVDEAELAERRKTWVPKERELKGVLARYARYAGQA
CCCCEEEEECCCEEEEEEHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
DKGAVPE
CCCCCCC
>Mature Secondary Structure
MRSDDVKSGYTRAPNRALIRSLGISDQEMKKPFVGIANSWNTIVPGHTHLRMVAERVREG
CCCCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
VAAGGGVAFEFNTIGICDGIAMGHEGMRYSLASRENIADSVELMIQAHRFDALVCICTCD
HCCCCCEEEEEECEEEECCHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEHH
KIVPGMLMAAARCNIPAIVVTGGNMLPGHHKGCELSLTDIFEGVGKVAARKMSEEELHEL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHH
EAAAMPGCGSCQGLYTANTMACMTEAMGMSLPGCAAIPAVDAAKLRIAYESGVRIVEMIK
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHCCCHHHHHHH
EDVKPRDIITKNSLKNAIAVDMALGGSTNTVLHLMAIAQEADVSLSLDDFTQMGATVPHI
HCCCCHHHEEHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCHH
CSMQPGGPHSMQTLYHSGGIPAVFKQIRPHLDDCMTVSGKTVFSIADGVKYVDENVIHSL
HCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHH
ENAVHKAGGLKILKGSLAPNGSVIKSAAVIDAMWKHQGPARVFDGENEAMDAILTGKIVE
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCEEEE
GDVIVIRYEGPKGGPGMPEMLSPTSALMGLGYTHVALVTDGRFSGGTRGPCIGHVAPEAK
CCEEEEEECCCCCCCCCCHHCCHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCCCC
AGGPIGLVREGDIIKIDLYEQTLDLLVDEAELAERRKTWVPKERELKGVLARYARYAGQA
CCCCEEEEECCCEEEEEEHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
DKGAVPE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA