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The map label for this gene is nlpE [H]

Identifier: 124265603

GI number: 124265603

Start: 444146

End: 444724

Strand: Direct

Name: nlpE [H]

Synonym: Mpe_A0410

Alternate gene names: 124265603

Gene position: 444146-444724 (Clockwise)

Preceding gene: 124265601

Following gene: 124265604

Centisome position: 10.98

GC content: 75.13

Gene sequence:

>579_bases
ATGGCGCTGGCGCATCGCGCGTCGGGACTGCGGCGGTGGCTCGCGCCGTGTCTGGCTTTCGTCCTGATGGGCGGAGCGCT
CGCCAGCGCGTGGGCGCAACCGACCCTGCCGGTGCCCGCCACTTTCGCCGGCGTCCTGCCCTGTGCCGACTGCGCCGGCG
TGGCGCAGACGCTCACGCTGCGTGCGGACGGCCTGTACCGCCTGCGCCGCACCTACCTGGGCAAGCCGGAAGGGCCGTTC
TCCGAACTTGGACACTGGAAGCTGAACGCCGCCGGCACGCTGCTGATGCTGCGCGGCGAGACGGACACGCTGCTGTTCGC
GGTGATGCCCTCGGGCGCCGAGGACGAGGGCGGGCTGCGCCTGCTCGACCGCCGCGGCCAGCCCATCGAGTCGGACGCCA
ACCTGGCCCTGCGCCGCACGGCGGCGCTCGACCCGGTGGCGTTCACCGACTGCGACGGCGGCGTGCGAGAGCGCGTCGCC
GACGATGGGCCGGGCAGCGCCGACGCCCCACCTGGCGTGGCCTGCGGGGCGCCGCAGGTGCCGGCGCACGATGCCCCGGA
GCCTCAGCCCAGGCACTGA

Upstream 100 bases:

>100_bases
GGTCGCGCCGTGACGGCCTGGAAATCGGCTACAAAGCGGTGGGAGCCAGTGGGGACTGGAGCGAGGGCGGTCGATGATTC
GAACAGTTGGCGGCGGCGCC

Downstream 100 bases:

>100_bases
CCACGCAACCGGAGGATCCCATGGACATCGACGCATTGCAGACCTTCCTCGGCACCACCGTGACTGCGGTGATCCTCAAG
ATCCTGGCCGCGCTCGCGTT

Product: hypothetical protein

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MALAHRASGLRRWLAPCLAFVLMGGALASAWAQPTLPVPATFAGVLPCADCAGVAQTLTLRADGLYRLRRTYLGKPEGPF
SELGHWKLNAAGTLLMLRGETDTLLFAVMPSGAEDEGGLRLLDRRGQPIESDANLALRRTAALDPVAFTDCDGGVRERVA
DDGPGSADAPPGVACGAPQVPAHDAPEPQPRH

Sequences:

>Translated_192_residues
MALAHRASGLRRWLAPCLAFVLMGGALASAWAQPTLPVPATFAGVLPCADCAGVAQTLTLRADGLYRLRRTYLGKPEGPF
SELGHWKLNAAGTLLMLRGETDTLLFAVMPSGAEDEGGLRLLDRRGQPIESDANLALRRTAALDPVAFTDCDGGVRERVA
DDGPGSADAPPGVACGAPQVPAHDAPEPQPRH
>Mature_191_residues
ALAHRASGLRRWLAPCLAFVLMGGALASAWAQPTLPVPATFAGVLPCADCAGVAQTLTLRADGLYRLRRTYLGKPEGPFS
ELGHWKLNAAGTLLMLRGETDTLLFAVMPSGAEDEGGLRLLDRRGQPIESDANLALRRTAALDPVAFTDCDGGVRERVAD
DGPGSADAPPGVACGAPQVPAHDAPEPQPRH

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=168, Percent_Identity=32.7380952380952, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 20111; Mature: 19980

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAHRASGLRRWLAPCLAFVLMGGALASAWAQPTLPVPATFAGVLPCADCAGVAQTLTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHH
RADGLYRLRRTYLGKPEGPFSELGHWKLNAAGTLLMLRGETDTLLFAVMPSGAEDEGGLR
CCCHHHHHHHHHCCCCCCCHHHCCCEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCEE
LLDRRGQPIESDANLALRRTAALDPVAFTDCDGGVRERVADDGPGSADAPPGVACGAPQV
EECCCCCCCCCCCCEEEHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
PAHDAPEPQPRH
CCCCCCCCCCCC
>Mature Secondary Structure 
ALAHRASGLRRWLAPCLAFVLMGGALASAWAQPTLPVPATFAGVLPCADCAGVAQTLTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHH
RADGLYRLRRTYLGKPEGPFSELGHWKLNAAGTLLMLRGETDTLLFAVMPSGAEDEGGLR
CCCHHHHHHHHHCCCCCCCHHHCCCEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCEE
LLDRRGQPIESDANLALRRTAALDPVAFTDCDGGVRERVADDGPGSADAPPGVACGAPQV
EECCCCCCCCCCCCEEEHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
PAHDAPEPQPRH
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]