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The map label for this gene is proA

Identifier: 124265462

GI number: 124265462

Start: 291834

End: 293123

Strand: Direct

Name: proA

Synonym: Mpe_A0269

Alternate gene names: 124265462

Gene position: 291834-293123 (Clockwise)

Preceding gene: 124265461

Following gene: 124265463

Centisome position: 7.22

GC content: 71.4

Gene sequence:

>1290_bases
ATGAACGCACCCGACGCCACCCCCGTGATCGCGCTGATGGACCGCCTGGGCAGCGCGGCACGCTCCGCATCGACGGCGAT
GGCCGCCGCATCGACGGCGGCGAAGAACGCGGCGCTGCTGGCGCTGGCCCGCGAGATCCGGGCCGGGGCTGCGCGGCTGG
CCGCGGAGAACGCGCGCGACCTCGACGTTGCTACCCGCGCCGGCCTGGCCGCGCCGATGGTCGACCGCCTGCGCCTCACC
GACAAGGTGATCGCGCTGATGGCCGAAGGCTGCGAGCAGGTCGCCGCGCTGCCCGACCCGATCGGTGAGATGACGAACCT
GCGCCGGCGCCCGAGCGGCATCACCGTCGGCCAGATGCGGGTGCCGCTGGGCGTGTTCGGCATGGTCTACGAGAGCCGGC
CGAACGTGACGATCGACGCCGCCTCGCTGGCCATCAAGAGCGGCAACGCCGCGATCCTGCGCGGTGGTTCCGAGGCGCTG
CATTCCAACACGGCGCTGATGGCGCTGGTGGTGCGCGCGCTGGCCGAGGCCGGGCTGCCAGGCGATGCCGTGCAACTGGT
GCCCACCACCGACCGCGCCGCGGTCGGCCGGCTGATCGCGATGCCGCAGTACGTCGACGTGATCATTCCGCGCGGCGGCA
AGGGCCTGATCGAGCGCATCGCCGCCGAGGCCACGGTGCCGGTCATCAAGCACCTGGACGGCAACTGCCACGTCTACGTC
GACGCTGGCGCGGACCTCGACCTCGCGGTGCGCGTGACCGACAACGCCAAGACCCAGAAGTACAGCCCCTGCAATGCGGC
CGAGTCGCTGCTGGTGCACCGAGACGTCGCGGCGGCCTTCCTGCCACGCATCGGCGCGGTGTTCGCGGCCAAGGGCGTGG
AGATGCGTTGCGGCCCGCGCGCCAAGGCGCTGCTGGCCGCGGTGCCGGGCGCGAAGCTGGTCGACGCGACCGAGGCCGAC
TGGGCCGAGGAGTACCTGGCGCCGGTCATCAGCATCAAGCTGGTCGACTCGCTCGACGAGGCGATCGCCCGCATCAACCG
CTACGGTTCGCACCACACCGACGCGATCCTGACCACGAACCACCCGAACGCGATGCGATTCCTGCGCGAGGTCGACTCGG
CCAGCGTGATGGTCAACGCCAGCACGCGCTTCGCCGATGGCTTCGAGTTCGGCCTGGGCGCCGAGATCGGCATCAGCACC
GACAAGTTCCACGCCCGCGGGCCGGTGGGCCTCGAGGGGCTCACCTCGATGAAGTGGGTGGTGTTCGGGCAGGGCGAGGT
CCGCACCTGA

Upstream 100 bases:

>100_bases
GCCTTCGCCGCCACGGCGCCGTTGTGGTTGCTGCTGACGCAGTGGCTGAGCTGAGCCCAATGGACCGGCAGGGCCGGCCC
TGCTTTGCGACAATCCGGTC

Downstream 100 bases:

>100_bases
TCGGCGCATGAGCCTCGATCCGCGCGGCGCCCTCGTCCTGTTCTCCGGCGGCCAGGACTCGACCGCCTGCCTGGCCTGGG
CGCTGGAGCACCACGCGCAC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNAPDATPVIALMDRLGSAARSASTAMAAASTAAKNAALLALAREIRAGAARLAAENARDLDVATRAGLAAPMVDRLRLT
DKVIALMAEGCEQVAALPDPIGEMTNLRRRPSGITVGQMRVPLGVFGMVYESRPNVTIDAASLAIKSGNAAILRGGSEAL
HSNTALMALVVRALAEAGLPGDAVQLVPTTDRAAVGRLIAMPQYVDVIIPRGGKGLIERIAAEATVPVIKHLDGNCHVYV
DAGADLDLAVRVTDNAKTQKYSPCNAAESLLVHRDVAAAFLPRIGAVFAAKGVEMRCGPRAKALLAAVPGAKLVDATEAD
WAEEYLAPVISIKLVDSLDEAIARINRYGSHHTDAILTTNHPNAMRFLREVDSASVMVNASTRFADGFEFGLGAEIGIST
DKFHARGPVGLEGLTSMKWVVFGQGEVRT

Sequences:

>Translated_429_residues
MNAPDATPVIALMDRLGSAARSASTAMAAASTAAKNAALLALAREIRAGAARLAAENARDLDVATRAGLAAPMVDRLRLT
DKVIALMAEGCEQVAALPDPIGEMTNLRRRPSGITVGQMRVPLGVFGMVYESRPNVTIDAASLAIKSGNAAILRGGSEAL
HSNTALMALVVRALAEAGLPGDAVQLVPTTDRAAVGRLIAMPQYVDVIIPRGGKGLIERIAAEATVPVIKHLDGNCHVYV
DAGADLDLAVRVTDNAKTQKYSPCNAAESLLVHRDVAAAFLPRIGAVFAAKGVEMRCGPRAKALLAAVPGAKLVDATEAD
WAEEYLAPVISIKLVDSLDEAIARINRYGSHHTDAILTTNHPNAMRFLREVDSASVMVNASTRFADGFEFGLGAEIGIST
DKFHARGPVGLEGLTSMKWVVFGQGEVRT
>Mature_429_residues
MNAPDATPVIALMDRLGSAARSASTAMAAASTAAKNAALLALAREIRAGAARLAAENARDLDVATRAGLAAPMVDRLRLT
DKVIALMAEGCEQVAALPDPIGEMTNLRRRPSGITVGQMRVPLGVFGMVYESRPNVTIDAASLAIKSGNAAILRGGSEAL
HSNTALMALVVRALAEAGLPGDAVQLVPTTDRAAVGRLIAMPQYVDVIIPRGGKGLIERIAAEATVPVIKHLDGNCHVYV
DAGADLDLAVRVTDNAKTQKYSPCNAAESLLVHRDVAAAFLPRIGAVFAAKGVEMRCGPRAKALLAAVPGAKLVDATEAD
WAEEYLAPVISIKLVDSLDEAIARINRYGSHHTDAILTTNHPNAMRFLREVDSASVMVNASTRFADGFEFGLGAEIGIST
DKFHARGPVGLEGLTSMKWVVFGQGEVRT

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=426, Percent_Identity=37.3239436619718, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI21361368, Length=426, Percent_Identity=37.3239436619718, Blast_Score=261, Evalue=9e-70,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=44.1247002398081, Blast_Score=346, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17569967, Length=419, Percent_Identity=37.9474940334129, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17569969, Length=419, Percent_Identity=37.9474940334129, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=38.2284382284382, Blast_Score=277, Evalue=2e-75,
Organism=Drosophila melanogaster, GI21357643, Length=409, Percent_Identity=41.320293398533, Blast_Score=282, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_METPP (A2SCE2)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019466.1
- ProteinModelPortal:   A2SCE2
- SMR:   A2SCE2
- STRING:   A2SCE2
- GeneID:   4786938
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0269
- NMPDR:   fig|279263.3.peg.185
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   GICGAME
- PhylomeDB:   A2SCE2
- ProtClustDB:   PRK00197
- BioCyc:   MPET420662:MPE_A0269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44948; Mature: 44948

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDATPVIALMDRLGSAARSASTAMAAASTAAKNAALLALAREIRAGAARLAAENARD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LDVATRAGLAAPMVDRLRLTDKVIALMAEGCEQVAALPDPIGEMTNLRRRPSGITVGQMR
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEE
VPLGVFGMVYESRPNVTIDAASLAIKSGNAAILRGGSEALHSNTALMALVVRALAEAGLP
CCHHHHHHEEECCCCEEEEHHEEEEECCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCCC
GDAVQLVPTTDRAAVGRLIAMPQYVDVIIPRGGKGLIERIAAEATVPVIKHLDGNCHVYV
CCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
DAGADLDLAVRVTDNAKTQKYSPCNAAESLLVHRDVAAAFLPRIGAVFAAKGVEMRCGPR
ECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AKALLAAVPGAKLVDATEADWAEEYLAPVISIKLVDSLDEAIARINRYGSHHTDAILTTN
HHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
HPNAMRFLREVDSASVMVNASTRFADGFEFGLGAEIGISTDKFHARGPVGLEGLTSMKWV
CHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEE
VFGQGEVRT
EEECCCCCC
>Mature Secondary Structure
MNAPDATPVIALMDRLGSAARSASTAMAAASTAAKNAALLALAREIRAGAARLAAENARD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LDVATRAGLAAPMVDRLRLTDKVIALMAEGCEQVAALPDPIGEMTNLRRRPSGITVGQMR
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEE
VPLGVFGMVYESRPNVTIDAASLAIKSGNAAILRGGSEALHSNTALMALVVRALAEAGLP
CCHHHHHHEEECCCCEEEEHHEEEEECCCEEEEECCHHHHHCCHHHHHHHHHHHHHCCCC
GDAVQLVPTTDRAAVGRLIAMPQYVDVIIPRGGKGLIERIAAEATVPVIKHLDGNCHVYV
CCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
DAGADLDLAVRVTDNAKTQKYSPCNAAESLLVHRDVAAAFLPRIGAVFAAKGVEMRCGPR
ECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AKALLAAVPGAKLVDATEADWAEEYLAPVISIKLVDSLDEAIARINRYGSHHTDAILTTN
HHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
HPNAMRFLREVDSASVMVNASTRFADGFEFGLGAEIGISTDKFHARGPVGLEGLTSMKWV
CHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEE
VFGQGEVRT
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA