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The map label for this gene is fixJ [H]

Identifier: 124265402

GI number: 124265402

Start: 226850

End: 227527

Strand: Direct

Name: fixJ [H]

Synonym: Mpe_A0209

Alternate gene names: 124265402

Gene position: 226850-227527 (Clockwise)

Preceding gene: 124265401

Following gene: 124265403

Centisome position: 5.61

GC content: 69.47

Gene sequence:

>678_bases
ATGAGCCATGGCGTGCTGCCGGCCACCGCGCCGCTGGGTCTGCCGGTCGTCCACCTCGTGGACGACGAGGCGGTGGTGCG
CAATGCGATCGCCTGGCTGTTGCGCACGCGCCGGCTGCTGGCCGAGCCGCACGAATCGGCCGAGGCTTTCGAGGCCATGC
TCGCGGCGCGCGGTGACGCGATCCTCAATGCTCCCACCTGCGTTCTGCTCGACATGCGCATGCCCGGTGGCATGAGCGGG
CTGCAGCTGTTCGAGCGGCTGATCGAGCAGCGCCTGCTGGCGGCGATGCCGGTCATCTTCCTCACCGGCCACGGTGACGT
GGCTACCGCGGTCGCGGCGATTCAGCAGGGCGCGTTCGACTTCGTCGAGAAGCCGTTCTCCGACAACAGCCTGGTCGACC
GCATCGAGCGGGCGCTGGCCGCCAGCCGCTCGACGCTCGAGGCGCGTCGCGCCGGCGCGCTGATGCGTCAGCGCCTGACC
GAGCTCACCGAGCGCGAGCGCGAGGTGATGATGCTGGTGATCGACGGCCGCCCGAACAAGCTGATCGCGCATGCACTCGA
CATCAGCGTGCGCACGGTCGAAGTGCACCGCGCGCGCGTTTTCGAGAAGATGGGCGTGAAGTCGGCCGTCGAACTGGCCA
ACGTGCTGCGCGAGCACGGCGCGCAGCGACCATCCTGA

Upstream 100 bases:

>100_bases
GCCGCGTGCGCGGCACCTGTTTCGAGTTCACACTTCCGGCTGCCGGGCCCGCTTCCTCCCCGGCGGCAGCCCCTCCGGGC
GTTCTCGATGGAGTCTCTGC

Downstream 100 bases:

>100_bases
ACCGGGCGCGGCCGCTGGCGTGGCGGCGCGGTGCGCGAGCGCCGAGGCCCGCGGCGACAATGCGGCCATGAGCCTCGGCG
AGTTCGACCTGATCGCGCGC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSHGVLPATAPLGLPVVHLVDDEAVVRNAIAWLLRTRRLLAEPHESAEAFEAMLAARGDAILNAPTCVLLDMRMPGGMSG
LQLFERLIEQRLLAAMPVIFLTGHGDVATAVAAIQQGAFDFVEKPFSDNSLVDRIERALAASRSTLEARRAGALMRQRLT
ELTEREREVMMLVIDGRPNKLIAHALDISVRTVEVHRARVFEKMGVKSAVELANVLREHGAQRPS

Sequences:

>Translated_225_residues
MSHGVLPATAPLGLPVVHLVDDEAVVRNAIAWLLRTRRLLAEPHESAEAFEAMLAARGDAILNAPTCVLLDMRMPGGMSG
LQLFERLIEQRLLAAMPVIFLTGHGDVATAVAAIQQGAFDFVEKPFSDNSLVDRIERALAASRSTLEARRAGALMRQRLT
ELTEREREVMMLVIDGRPNKLIAHALDISVRTVEVHRARVFEKMGVKSAVELANVLREHGAQRPS
>Mature_224_residues
SHGVLPATAPLGLPVVHLVDDEAVVRNAIAWLLRTRRLLAEPHESAEAFEAMLAARGDAILNAPTCVLLDMRMPGGMSGL
QLFERLIEQRLLAAMPVIFLTGHGDVATAVAAIQQGAFDFVEKPFSDNSLVDRIERALAASRSTLEARRAGALMRQRLTE
LTEREREVMMLVIDGRPNKLIAHALDISVRTVEVHRARVFEKMGVKSAVELANVLREHGAQRPS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=139, Percent_Identity=37.410071942446, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=33.502538071066, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=28.3018867924528, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788905, Length=82, Percent_Identity=42.6829268292683, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=30.8943089430894, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24679; Mature: 24548

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHGVLPATAPLGLPVVHLVDDEAVVRNAIAWLLRTRRLLAEPHESAEAFEAMLAARGDA
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
ILNAPTCVLLDMRMPGGMSGLQLFERLIEQRLLAAMPVIFLTGHGDVATAVAAIQQGAFD
EECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHH
FVEKPFSDNSLVDRIERALAASRSTLEARRAGALMRQRLTELTEREREVMMLVIDGRPNK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
LIAHALDISVRTVEVHRARVFEKMGVKSAVELANVLREHGAQRPS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SHGVLPATAPLGLPVVHLVDDEAVVRNAIAWLLRTRRLLAEPHESAEAFEAMLAARGDA
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
ILNAPTCVLLDMRMPGGMSGLQLFERLIEQRLLAAMPVIFLTGHGDVATAVAAIQQGAFD
EECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHH
FVEKPFSDNSLVDRIERALAASRSTLEARRAGALMRQRLTELTEREREVMMLVIDGRPNK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
LIAHALDISVRTVEVHRARVFEKMGVKSAVELANVLREHGAQRPS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]