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The map label for this gene is lcfB [H]

Identifier: 124265370

GI number: 124265370

Start: 191933

End: 193477

Strand: Direct

Name: lcfB [H]

Synonym: Mpe_A0177

Alternate gene names: 124265370

Gene position: 191933-193477 (Clockwise)

Preceding gene: 124265369

Following gene: 124265371

Centisome position: 4.75

GC content: 68.8

Gene sequence:

>1545_bases
ATGCGACTGGTCGACTACCTCGACAAGGGCGCCCAACTCGGCGCTGACGCGCCCTGCCTGACCATGGGCGACGCCGACCT
GAGCTACGCCGACGTGCAGCGCATCAGCTACCGCGTCGCCCGCGGCCTGCAACGCTCGGGCATCGCACCGGGCGACAAGG
TCGCGGTGCTGTCGAGCAACCACGCGCTGGCCTTCGCCTGTGTGTTCGGCATCGCGCGCGCCGGCGCCGTGTGGTGCCCG
ATCAACCCGCGCAACGAGGCGAGCGAGAACCGCTACGTGCTCGACGCCTTCGACTGCGCCTGTGTCATCTTTCACAGCAA
CTATGCGCCGATGGTCGAGCAGATGCGCGCCGGCCTGCCCAAGCTGCGGCTGCTGGTCTGCCTCGATCAGCCGGCGCCGT
TCGCGGCGGGCTTCGGCGACTGGCTGCATGGTCTTGGCGACGAGCCGCTGCACGTCGAGCCGCCCGACGACCTGGCGATG
ATCGCGGGCACCGGCGGCACCACCGGCAGTCCCAAGGGCGTGATGCTGACCGGCCGCAACCTCGAGACGATGTCGGCGCT
GACGCTGATGGGCTATCCGTTCGACGGGCGGCCTTCCTACCTGGCGCTGGCGCCGCTGACCCATGCGGCCGGCGTGCTGT
GCCTGCCGGTGATGGCGCTGGGTGGCCGCGTGGTCATCATGCCGAAGCCCGATCTGGGCGAGTTCCTCGCGCTGATCGAG
CGCCACCGCATCACCCACACCTTCCTGCCGCCGACGCTGATCTACATGCTGCTGCAGCACGAGCGCCTGGCCACGGCGAG
GCTCGATTCGCTGCAGTGCTTCTGGTATGGCGCGGCGCCGATCTCGGCGGCGCGGCTCGAGGAGGCGTTGACCAAGATCG
GCCCGGTGATGGCGCAGCTGTTCGGGCAGACCGAGGCGCCGATGATGATCTCGATGATGTCGCCCCGCGAGCACTTCCAG
ACCGACGGCGCGGTAGCGCGCGCGCGCCTGTCGTCGGCCGGCCGGCCCGGCCCGCTGGTCCAGGTGGCCACGATGGACGG
CGAGGGCCGCCTGCTGCCCACCGGCGAGACCGGCGAGATCGTGGTGCGCGGCTCATTGGTGATGGCCGGCTACTACAAGG
ATCCCAAGGCCACGGCCGAGGCCGGCCGCCACGGATGGCACCACACCGGCGACATCGGCCGGCTCGATGCCGACGGCTTT
CTCTTCATCGTCGACCGCGCCAAGGACATGATCATCACCGGCGGCTTCAACGTCTACTCGGTCGAGGTCGAGCAGGCGCT
GATGCAGCATCCCGACGTGCAGGACAGCGCGGTGATCGGTCTGCCCGACGACAAGTGGGGTGAGCGCGTGGTGGCGGTGT
TGCAGCTGCACGCCGGCCGCAGCGTGAAGCCCGAGGACATCCAGGCCTTCGTCAAGGCCCGCATCGGCAGCGTCAAGTCG
CCCAAGCAGGTCGAGATCTGGTCCGACCTGCCACGTTCCAAGGTCGGCAAGGTCTTGAAGAAGGACATCCGTGCCTCGTT
GCTGAAGCCTGCAGAATCGAGTTGA

Upstream 100 bases:

>100_bases
CCGGCTGCCAGGCCTTCGGACATCACGAACTGCTCGGCGCCCTGCGCAGCTTCGGCCTGGTGCAGGCGCCGATCCTGACC
GTCCACCGCTAGGCCGCGCG

Downstream 100 bases:

>100_bases
GCTGCCTTCCAGCCACCGCACCCATCGGAGAGCCGCCATGGCCAACAGAGTCTGCATTGCCGGCGTGGGCATGATCGCCT
TCGCCAAGCCCGGCGCCAGC

Product: putative ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MRLVDYLDKGAQLGADAPCLTMGDADLSYADVQRISYRVARGLQRSGIAPGDKVAVLSSNHALAFACVFGIARAGAVWCP
INPRNEASENRYVLDAFDCACVIFHSNYAPMVEQMRAGLPKLRLLVCLDQPAPFAAGFGDWLHGLGDEPLHVEPPDDLAM
IAGTGGTTGSPKGVMLTGRNLETMSALTLMGYPFDGRPSYLALAPLTHAAGVLCLPVMALGGRVVIMPKPDLGEFLALIE
RHRITHTFLPPTLIYMLLQHERLATARLDSLQCFWYGAAPISAARLEEALTKIGPVMAQLFGQTEAPMMISMMSPREHFQ
TDGAVARARLSSAGRPGPLVQVATMDGEGRLLPTGETGEIVVRGSLVMAGYYKDPKATAEAGRHGWHHTGDIGRLDADGF
LFIVDRAKDMIITGGFNVYSVEVEQALMQHPDVQDSAVIGLPDDKWGERVVAVLQLHAGRSVKPEDIQAFVKARIGSVKS
PKQVEIWSDLPRSKVGKVLKKDIRASLLKPAESS

Sequences:

>Translated_514_residues
MRLVDYLDKGAQLGADAPCLTMGDADLSYADVQRISYRVARGLQRSGIAPGDKVAVLSSNHALAFACVFGIARAGAVWCP
INPRNEASENRYVLDAFDCACVIFHSNYAPMVEQMRAGLPKLRLLVCLDQPAPFAAGFGDWLHGLGDEPLHVEPPDDLAM
IAGTGGTTGSPKGVMLTGRNLETMSALTLMGYPFDGRPSYLALAPLTHAAGVLCLPVMALGGRVVIMPKPDLGEFLALIE
RHRITHTFLPPTLIYMLLQHERLATARLDSLQCFWYGAAPISAARLEEALTKIGPVMAQLFGQTEAPMMISMMSPREHFQ
TDGAVARARLSSAGRPGPLVQVATMDGEGRLLPTGETGEIVVRGSLVMAGYYKDPKATAEAGRHGWHHTGDIGRLDADGF
LFIVDRAKDMIITGGFNVYSVEVEQALMQHPDVQDSAVIGLPDDKWGERVVAVLQLHAGRSVKPEDIQAFVKARIGSVKS
PKQVEIWSDLPRSKVGKVLKKDIRASLLKPAESS
>Mature_514_residues
MRLVDYLDKGAQLGADAPCLTMGDADLSYADVQRISYRVARGLQRSGIAPGDKVAVLSSNHALAFACVFGIARAGAVWCP
INPRNEASENRYVLDAFDCACVIFHSNYAPMVEQMRAGLPKLRLLVCLDQPAPFAAGFGDWLHGLGDEPLHVEPPDDLAM
IAGTGGTTGSPKGVMLTGRNLETMSALTLMGYPFDGRPSYLALAPLTHAAGVLCLPVMALGGRVVIMPKPDLGEFLALIE
RHRITHTFLPPTLIYMLLQHERLATARLDSLQCFWYGAAPISAARLEEALTKIGPVMAQLFGQTEAPMMISMMSPREHFQ
TDGAVARARLSSAGRPGPLVQVATMDGEGRLLPTGETGEIVVRGSLVMAGYYKDPKATAEAGRHGWHHTGDIGRLDADGF
LFIVDRAKDMIITGGFNVYSVEVEQALMQHPDVQDSAVIGLPDDKWGERVVAVLQLHAGRSVKPEDIQAFVKARIGSVKS
PKQVEIWSDLPRSKVGKVLKKDIRASLLKPAESS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=28.5171102661597, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI122937307, Length=507, Percent_Identity=27.4161735700197, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI187761345, Length=530, Percent_Identity=27.3584905660377, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI187761343, Length=530, Percent_Identity=27.3584905660377, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI38505220, Length=510, Percent_Identity=25.6862745098039, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=23.4375, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=24.0157480314961, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI157311624, Length=484, Percent_Identity=25.4132231404959, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI157311622, Length=484, Percent_Identity=25.4132231404959, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI58082049, Length=482, Percent_Identity=25.7261410788382, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI28416953, Length=538, Percent_Identity=23.6059479553903, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI8923896, Length=415, Percent_Identity=25.3012048192771, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI27477105, Length=191, Percent_Identity=35.0785340314136, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI253970489, Length=408, Percent_Identity=25.2450980392157, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI40807491, Length=516, Percent_Identity=24.6124031007752, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=30.2973977695167, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI221142682, Length=489, Percent_Identity=28.4253578732106, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI145693145, Length=530, Percent_Identity=28.4905660377358, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786810, Length=522, Percent_Identity=27.2030651340996, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790505, Length=543, Percent_Identity=24.8618784530387, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1786801, Length=439, Percent_Identity=23.6902050113895, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786529, Length=390, Percent_Identity=26.1538461538462, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788595, Length=338, Percent_Identity=25.7396449704142, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994690, Length=518, Percent_Identity=27.7992277992278, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71994694, Length=518, Percent_Identity=27.7992277992278, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71994703, Length=518, Percent_Identity=27.992277992278, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17559526, Length=537, Percent_Identity=28.6778398510242, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=28.8844621513944, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=28.3132530120482, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17560308, Length=527, Percent_Identity=29.6015180265655, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531443, Length=506, Percent_Identity=26.0869565217391, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560140, Length=490, Percent_Identity=24.2857142857143, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=503, Percent_Identity=25.2485089463221, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=532, Percent_Identity=24.6240601503759, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71996755, Length=494, Percent_Identity=24.6963562753036, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=27.9329608938547, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556552, Length=525, Percent_Identity=22.6666666666667, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141359, Length=511, Percent_Identity=21.9178082191781, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=381, Percent_Identity=24.9343832020997, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=381, Percent_Identity=24.9343832020997, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17564090, Length=343, Percent_Identity=23.9067055393586, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=378, Percent_Identity=27.7777777777778, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=562, Percent_Identity=24.1992882562278, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=391, Percent_Identity=23.0179028132992, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=488, Percent_Identity=29.9180327868852, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24581924, Length=537, Percent_Identity=26.6294227188082, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859661, Length=366, Percent_Identity=30.8743169398907, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=24.4488977955912, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21358303, Length=470, Percent_Identity=24.468085106383, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648257, Length=377, Percent_Identity=27.5862068965517, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356441, Length=516, Percent_Identity=25, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648260, Length=507, Percent_Identity=24.8520710059172, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24653035, Length=363, Percent_Identity=25.8953168044077, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648255, Length=368, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648253, Length=368, Percent_Identity=24.7282608695652, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922652, Length=466, Percent_Identity=24.8927038626609, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161076582, Length=373, Percent_Identity=25.7372654155496, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24656500, Length=506, Percent_Identity=22.9249011857708, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281365686, Length=467, Percent_Identity=22.9122055674518, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19921316, Length=515, Percent_Identity=25.4368932038835, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20130357, Length=506, Percent_Identity=22.7272727272727, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281366413, Length=317, Percent_Identity=28.0757097791798, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666501, Length=317, Percent_Identity=28.0757097791798, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24666497, Length=317, Percent_Identity=28.0757097791798, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62472339, Length=378, Percent_Identity=25.1322751322751, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667955, Length=378, Percent_Identity=25.1322751322751, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=25.1322751322751, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55452; Mature: 55452

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVDYLDKGAQLGADAPCLTMGDADLSYADVQRISYRVARGLQRSGIAPGDKVAVLSSN
CCHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HALAFACVFGIARAGAVWCPINPRNEASENRYVLDAFDCACVIFHSNYAPMVEQMRAGLP
CCHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCHHHHHHHHCCCC
KLRLLVCLDQPAPFAAGFGDWLHGLGDEPLHVEPPDDLAMIAGTGGTTGSPKGVMLTGRN
CEEEEEEECCCCCCCCCHHHHHHCCCCCCEECCCCCCEEEEEECCCCCCCCCEEEEECCC
LETMSALTLMGYPFDGRPSYLALAPLTHAAGVLCLPVMALGGRVVIMPKPDLGEFLALIE
CHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
RHRITHTFLPPTLIYMLLQHERLATARLDSLQCFWYGAAPISAARLEEALTKIGPVMAQL
HHCCCEECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
FGQTEAPMMISMMSPREHFQTDGAVARARLSSAGRPGPLVQVATMDGEGRLLPTGETGEI
HCCCCCCEEEEECCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCE
VVRGSLVMAGYYKDPKATAEAGRHGWHHTGDIGRLDADGFLFIVDRAKDMIITGGFNVYS
EEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCCEECCCCEEEEEECCCCEEEECCCEEEE
VEVEQALMQHPDVQDSAVIGLPDDKWGERVVAVLQLHAGRSVKPEDIQAFVKARIGSVKS
EHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PKQVEIWSDLPRSKVGKVLKKDIRASLLKPAESS
CCEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLVDYLDKGAQLGADAPCLTMGDADLSYADVQRISYRVARGLQRSGIAPGDKVAVLSSN
CCHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
HALAFACVFGIARAGAVWCPINPRNEASENRYVLDAFDCACVIFHSNYAPMVEQMRAGLP
CCHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCHHHHHHHHCCCC
KLRLLVCLDQPAPFAAGFGDWLHGLGDEPLHVEPPDDLAMIAGTGGTTGSPKGVMLTGRN
CEEEEEEECCCCCCCCCHHHHHHCCCCCCEECCCCCCEEEEEECCCCCCCCCEEEEECCC
LETMSALTLMGYPFDGRPSYLALAPLTHAAGVLCLPVMALGGRVVIMPKPDLGEFLALIE
CHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHH
RHRITHTFLPPTLIYMLLQHERLATARLDSLQCFWYGAAPISAARLEEALTKIGPVMAQL
HHCCCEECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
FGQTEAPMMISMMSPREHFQTDGAVARARLSSAGRPGPLVQVATMDGEGRLLPTGETGEI
HCCCCCCEEEEECCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCE
VVRGSLVMAGYYKDPKATAEAGRHGWHHTGDIGRLDADGFLFIVDRAKDMIITGGFNVYS
EEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCCEECCCCEEEEEECCCCEEEECCCEEEE
VEVEQALMQHPDVQDSAVIGLPDDKWGERVVAVLQLHAGRSVKPEDIQAFVKARIGSVKS
EHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PKQVEIWSDLPRSKVGKVLKKDIRASLLKPAESS
CCEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]