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The map label for this gene is purF [H]

Identifier: 124021718

GI number: 124021718

Start: 4653

End: 6110

Strand: Direct

Name: purF [H]

Synonym: P9303_00031

Alternate gene names: 124021718

Gene position: 4653-6110 (Clockwise)

Preceding gene: 124021717

Following gene: 124021722

Centisome position: 0.17

GC content: 42.52

Gene sequence:

>1458_bases
ATGTGCGGCATTGTTGGCATTGTTTCTACTGCGCTGGTCAATCAGCAGATTTATGACAGCTTGCTGCTTCTACAGCACCG
GGGTCAGGACTCTACTGGCATAGCCACCATGGATGGCAGTGTTTTTCACTTGCATAAGGCAAGGGGGCAAGTGCGGGAGG
CCTATCGCACACGTGATATGCGTTCCTTAATCGGTAATATTGGTCTTGGTCATGTCAGATATGCAACTAAGGGTGCAGCT
GAGCGGGAAGAAGAAGCACAACCATTTTATGTGAATGCTCCTTACGGAATCATTCTTATTCATAACGGTAACCTTACCAA
TACTCGAGAGCTTGATCGCGACTTGTTCAATATTGATCGTCGCCATACAAATTCAAAGAGTGATACGGAGATGTTGCTTA
ATGTTCTAGCTACTGAATTGCAATTGAAAATTTATGGTAGTGATCTTACTCCAGATCAGGTATTTGATGCTGTAACGTCT
GTACATCAACGAGTAGAAGGTTCTTATACATCTATTGCTTTGATTGCAGGGCACGGTCTTCTTGCCTTTCGCGATCCGTT
TGGGATTAGGCCATTGGTCCTTGGTAAACGTAAGTCTTTAGAAGGTGCTGATGAGTGGATTGTGGCTAGTGAGTCATTGG
TATTAGAGAATGGTGATTATGAGATTGTTAGGGATGTTGATCCTGGAGAGGCTATTTTTATTACTCAAAATGGTGAGCTT
TATTCACGTCAGTGTGCGAAGAATCCGCGTCTTGTGCCATGTTCATTTGAGTATGTTTATCTTGCTCGACCTGATTCAGT
GATGAGTGGAATCTCGGTTTACGAGGCTCGATTAAGGATGGGGGATCGACTTGCAGAAACTATTGCAAAGTACACGCCAT
CTGGCGATATAGATGTTGTGATGCCAATTCCTGACTCTTCACGGCCTGCTGCTATGCAAGTGGCTAGACAGCTTGGGATT
GAATATCGTGAGGGCTTTTTTAAAAATCGTTATGTAGGCCGTACATTTATTATGCCTGGACAAGCTCAACGTAAGAAATC
TGTTAGACAAAAGTTAAATGCTATGGGAACAGAATTTAAAGGTAAGAATGTTTTAATTGTGGATGATTCAATTGTACGTG
GTACGACTTCGAAGGAGATAGTACAAATGGCTCGAGTTGCCGGTGCGAATAAAGTTACGTTTACTTCTGCAGCACCACCA
GTGCGATATTCACATGTTTATGGCATAAATATGCCAAATCGGCATGAGCTGATTGCTTATAATCGCACTATTCCTGAAAT
CTCTTCTGAACTTTCCACTGATCACATGGTCTATCAAGAGGTTGCTGATCTAGAAGCAGCAATTGTTGAAGGTTCAGGTG
TTTCTGAACTTGAGCTTTCTTGTTTTACCGGAGAGTATATTACAGGGACAGTTACTTCTGAATATCTCGATTGGATAGAA
CGCACACAATTGTCTTAA

Upstream 100 bases:

>100_bases
TCCCTCGTCGTATGGGCTTGGATCTCTCTTCAAGCGTCTGAGTTGAGCTTTAGTGCAAAATGTCTTCCTTGGCTTATGCA
TTTGAGGAGTTGAGCTTCAC

Downstream 100 bases:

>100_bases
TAAAGGTGATTATCAAGGCCTTAAGTGTTAAATGCTTAATCTATAATATTATTAGTCGTGATTAGTGGCACGAGTTTTTG
AATTTCTTCGTTGTCTTTTA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MCGIVGIVSTALVNQQIYDSLLLLQHRGQDSTGIATMDGSVFHLHKARGQVREAYRTRDMRSLIGNIGLGHVRYATKGAA
EREEEAQPFYVNAPYGIILIHNGNLTNTRELDRDLFNIDRRHTNSKSDTEMLLNVLATELQLKIYGSDLTPDQVFDAVTS
VHQRVEGSYTSIALIAGHGLLAFRDPFGIRPLVLGKRKSLEGADEWIVASESLVLENGDYEIVRDVDPGEAIFITQNGEL
YSRQCAKNPRLVPCSFEYVYLARPDSVMSGISVYEARLRMGDRLAETIAKYTPSGDIDVVMPIPDSSRPAAMQVARQLGI
EYREGFFKNRYVGRTFIMPGQAQRKKSVRQKLNAMGTEFKGKNVLIVDDSIVRGTTSKEIVQMARVAGANKVTFTSAAPP
VRYSHVYGINMPNRHELIAYNRTIPEISSELSTDHMVYQEVADLEAAIVEGSGVSELELSCFTGEYITGTVTSEYLDWIE
RTQLS

Sequences:

>Translated_485_residues
MCGIVGIVSTALVNQQIYDSLLLLQHRGQDSTGIATMDGSVFHLHKARGQVREAYRTRDMRSLIGNIGLGHVRYATKGAA
EREEEAQPFYVNAPYGIILIHNGNLTNTRELDRDLFNIDRRHTNSKSDTEMLLNVLATELQLKIYGSDLTPDQVFDAVTS
VHQRVEGSYTSIALIAGHGLLAFRDPFGIRPLVLGKRKSLEGADEWIVASESLVLENGDYEIVRDVDPGEAIFITQNGEL
YSRQCAKNPRLVPCSFEYVYLARPDSVMSGISVYEARLRMGDRLAETIAKYTPSGDIDVVMPIPDSSRPAAMQVARQLGI
EYREGFFKNRYVGRTFIMPGQAQRKKSVRQKLNAMGTEFKGKNVLIVDDSIVRGTTSKEIVQMARVAGANKVTFTSAAPP
VRYSHVYGINMPNRHELIAYNRTIPEISSELSTDHMVYQEVADLEAAIVEGSGVSELELSCFTGEYITGTVTSEYLDWIE
RTQLS
>Mature_485_residues
MCGIVGIVSTALVNQQIYDSLLLLQHRGQDSTGIATMDGSVFHLHKARGQVREAYRTRDMRSLIGNIGLGHVRYATKGAA
EREEEAQPFYVNAPYGIILIHNGNLTNTRELDRDLFNIDRRHTNSKSDTEMLLNVLATELQLKIYGSDLTPDQVFDAVTS
VHQRVEGSYTSIALIAGHGLLAFRDPFGIRPLVLGKRKSLEGADEWIVASESLVLENGDYEIVRDVDPGEAIFITQNGEL
YSRQCAKNPRLVPCSFEYVYLARPDSVMSGISVYEARLRMGDRLAETIAKYTPSGDIDVVMPIPDSSRPAAMQVARQLGI
EYREGFFKNRYVGRTFIMPGQAQRKKSVRQKLNAMGTEFKGKNVLIVDDSIVRGTTSKEIVQMARVAGANKVTFTSAAPP
VRYSHVYGINMPNRHELIAYNRTIPEISSELSTDHMVYQEVADLEAAIVEGSGVSELELSCFTGEYITGTVTSEYLDWIE
RTQLS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=480, Percent_Identity=35.625, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1788651, Length=482, Percent_Identity=53.5269709543568, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790167, Length=224, Percent_Identity=30.8035714285714, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=32.9192546583851, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6323958, Length=493, Percent_Identity=47.4645030425963, Blast_Score=441, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24659598, Length=491, Percent_Identity=35.030549898167, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24659604, Length=483, Percent_Identity=35.1966873706004, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI28573187, Length=511, Percent_Identity=33.0724070450098, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53971; Mature: 53971

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVSTALVNQQIYDSLLLLQHRGQDSTGIATMDGSVFHLHKARGQVREAYRTRDM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHH
RSLIGNIGLGHVRYATKGAAEREEEAQPFYVNAPYGIILIHNGNLTNTRELDRDLFNIDR
HHHHHCCCCCCEEECCCCCCCHHHCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHTNSKSDTEMLLNVLATELQLKIYGSDLTPDQVFDAVTSVHQRVEGSYTSIALIAGHGL
HCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCE
LAFRDPFGIRPLVLGKRKSLEGADEWIVASESLVLENGDYEIVRDVDPGEAIFITQNGEL
EEECCCCCCCCEEECCCCCCCCCCCEEEECCEEEEECCCEEEEEECCCCCEEEEEECCCH
YSRQCAKNPRLVPCSFEYVYLARPDSVMSGISVYEARLRMGDRLAETIAKYTPSGDIDVV
HHHHHCCCCCEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQAQRKKSVRQKLNAMGTEFK
EECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCC
GKNVLIVDDSIVRGTTSKEIVQMARVAGANKVTFTSAAPPVRYSHVYGINMPNRHELIAY
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEE
NRTIPEISSELSTDHMVYQEVADLEAAIVEGSGVSELELSCFTGEYITGTVTSEYLDWIE
CCCCHHHHHHHCHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCEEEECHHHHHHHHHH
RTQLS
HHCCC
>Mature Secondary Structure
MCGIVGIVSTALVNQQIYDSLLLLQHRGQDSTGIATMDGSVFHLHKARGQVREAYRTRDM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHH
RSLIGNIGLGHVRYATKGAAEREEEAQPFYVNAPYGIILIHNGNLTNTRELDRDLFNIDR
HHHHHCCCCCCEEECCCCCCCHHHCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHTNSKSDTEMLLNVLATELQLKIYGSDLTPDQVFDAVTSVHQRVEGSYTSIALIAGHGL
HCCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCE
LAFRDPFGIRPLVLGKRKSLEGADEWIVASESLVLENGDYEIVRDVDPGEAIFITQNGEL
EEECCCCCCCCEEECCCCCCCCCCCEEEECCEEEEECCCEEEEEECCCCCEEEEEECCCH
YSRQCAKNPRLVPCSFEYVYLARPDSVMSGISVYEARLRMGDRLAETIAKYTPSGDIDVV
HHHHHCCCCCEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQAQRKKSVRQKLNAMGTEFK
EECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCC
GKNVLIVDDSIVRGTTSKEIVQMARVAGANKVTFTSAAPPVRYSHVYGINMPNRHELIAY
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEE
NRTIPEISSELSTDHMVYQEVADLEAAIVEGSGVSELELSCFTGEYITGTVTSEYLDWIE
CCCCHHHHHHHCHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCEEEECHHHHHHHHHH
RTQLS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]