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The map label for this gene is rfbD [H]

Identifier: 124025563

GI number: 124025563

Start: 785447

End: 786340

Strand: Direct

Name: rfbD [H]

Synonym: NATL1_08561

Alternate gene names: 124025563

Gene position: 785447-786340 (Clockwise)

Preceding gene: 124025562

Following gene: 124025564

Centisome position: 42.12

GC content: 39.04

Gene sequence:

>894_bases
ATGAAAGTTTTACTCACAGGAGCATCAGGACAACTAGGGCAAGCAATCATTAAATCAAAGCCATCTTTTGTTGAGCTAAT
AGCCACAACTCGACGTGAACTCGATTTAGCTGATGATGAAGCATGCCGTAGAGCAGTAAGACAGCATCAGCCAGATTGGG
TCATCAATAGTGGTGCATATACAGCTGTAGACAAGGCAGAAGACGAAAAAGAATTAGCAATGAGTATTAACACAATCGCT
CCAAAAATGTTTGCAGAGGAATTGAGTCAAACTGGAGGGAAGTTATTACAACTAAGTACTGATTTTGTATTTGATGGGGA
ACAAAATTTTCCATACAAAACTGGTCAAAAGAAGAAACCCTTGGGAGTTTATGGAGCAACTAAAGCAGCAGGTGAAGATG
CTATTTACGATGTACTAGGGAATTCCAACCAAGCTTTGATCATTCGAACGAGTTGGTTATTTGGGGCTGTTGGAAAGAAT
TTCATGAAGACGATGATTAGGCTACATCGGTCTCAAGAAGAAATTCGTGTCGTTTCAGATCAAATTGGTTGTCCAACAAG
CACTTTGACATTAGCTAAAGCATGCTGGAGGGCAATTGAGAAACAAAAAGATATTGAGCCAAACGTATTATCTCCTCAAC
TTCACCATTGGAGTGATGAAGGCTCAGCAAGTTGGTATGAGTTTGCAAAGGTAATTGGTGAATTAGGTTGTAGGATAGGC
TTAATAGAAAGACCAGCCAAAGTTATACCAATAAGTAGTGAGGAGTATACGACACGTGCTAAAAGACCTAAATATTCAAT
TTTGGATTGTACAACTACAAGACAATTTCTTAATCTTGAAGGCATTCATTGGAAAGAGAGCATCCTCAATGTGATGAAGC
AAGTTTCAACTTGA

Upstream 100 bases:

>100_bases
CTTGGCCTATAAAGGAGTTTGGCTTTGAACCAGTCATTAACGAAAAAGATGCATCTGCTCCTTTCTTGAAAGAGTCAATA
ATGAAAGGGAACATATTTTA

Downstream 100 bases:

>100_bases
TAAAAACATATCAACATTCATAATTATGGAAAAAAATTATTTTTTAAATGAACTTCTAGGAAATCGGAGAAGGATTCTAG
TTACTGGAGGAGCAGGCTTT

Product: putative dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKVLLTGASGQLGQAIIKSKPSFVELIATTRRELDLADDEACRRAVRQHQPDWVINSGAYTAVDKAEDEKELAMSINTIA
PKMFAEELSQTGGKLLQLSTDFVFDGEQNFPYKTGQKKKPLGVYGATKAAGEDAIYDVLGNSNQALIIRTSWLFGAVGKN
FMKTMIRLHRSQEEIRVVSDQIGCPTSTLTLAKACWRAIEKQKDIEPNVLSPQLHHWSDEGSASWYEFAKVIGELGCRIG
LIERPAKVIPISSEEYTTRAKRPKYSILDCTTTRQFLNLEGIHWKESILNVMKQVST

Sequences:

>Translated_297_residues
MKVLLTGASGQLGQAIIKSKPSFVELIATTRRELDLADDEACRRAVRQHQPDWVINSGAYTAVDKAEDEKELAMSINTIA
PKMFAEELSQTGGKLLQLSTDFVFDGEQNFPYKTGQKKKPLGVYGATKAAGEDAIYDVLGNSNQALIIRTSWLFGAVGKN
FMKTMIRLHRSQEEIRVVSDQIGCPTSTLTLAKACWRAIEKQKDIEPNVLSPQLHHWSDEGSASWYEFAKVIGELGCRIG
LIERPAKVIPISSEEYTTRAKRPKYSILDCTTTRQFLNLEGIHWKESILNVMKQVST
>Mature_297_residues
MKVLLTGASGQLGQAIIKSKPSFVELIATTRRELDLADDEACRRAVRQHQPDWVINSGAYTAVDKAEDEKELAMSINTIA
PKMFAEELSQTGGKLLQLSTDFVFDGEQNFPYKTGQKKKPLGVYGATKAAGEDAIYDVLGNSNQALIIRTSWLFGAVGKN
FMKTMIRLHRSQEEIRVVSDQIGCPTSTLTLAKACWRAIEKQKDIEPNVLSPQLHHWSDEGSASWYEFAKVIGELGCRIG
LIERPAKVIPISSEEYTTRAKRPKYSILDCTTTRQFLNLEGIHWKESILNVMKQVST

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=290, Percent_Identity=27.2413793103448, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI11034825, Length=290, Percent_Identity=27.2413793103448, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1788352, Length=301, Percent_Identity=32.890365448505, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33137; Mature: 33137

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLTGASGQLGQAIIKSKPSFVELIATTRRELDLADDEACRRAVRQHQPDWVINSGAY
CEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCE
TAVDKAEDEKELAMSINTIAPKMFAEELSQTGGKLLQLSTDFVFDGEQNFPYKTGQKKKP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCCCCCCC
LGVYGATKAAGEDAIYDVLGNSNQALIIRTSWLFGAVGKNFMKTMIRLHRSQEEIRVVSD
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QIGCPTSTLTLAKACWRAIEKQKDIEPNVLSPQLHHWSDEGSASWYEFAKVIGELGCRIG
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCEEE
LIERPAKVIPISSEEYTTRAKRPKYSILDCTTTRQFLNLEGIHWKESILNVMKQVST
EECCCCEEEECCCHHHHHHHCCCCCEEEECHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLLTGASGQLGQAIIKSKPSFVELIATTRRELDLADDEACRRAVRQHQPDWVINSGAY
CEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCE
TAVDKAEDEKELAMSINTIAPKMFAEELSQTGGKLLQLSTDFVFDGEQNFPYKTGQKKKP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCCCCCCC
LGVYGATKAAGEDAIYDVLGNSNQALIIRTSWLFGAVGKNFMKTMIRLHRSQEEIRVVSD
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QIGCPTSTLTLAKACWRAIEKQKDIEPNVLSPQLHHWSDEGSASWYEFAKVIGELGCRIG
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCEEE
LIERPAKVIPISSEEYTTRAKRPKYSILDCTTTRQFLNLEGIHWKESILNVMKQVST
EECCCCEEEECCCHHHHHHHCCCCCEEEECHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]