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The map label for this gene is rfbB [H]

Identifier: 124025564

GI number: 124025564

Start: 786366

End: 787442

Strand: Direct

Name: rfbB [H]

Synonym: NATL1_08571

Alternate gene names: 124025564

Gene position: 786366-787442 (Clockwise)

Preceding gene: 124025563

Following gene: 124025574

Centisome position: 42.17

GC content: 35.47

Gene sequence:

>1077_bases
ATGGAAAAAAATTATTTTTTAAATGAACTTCTAGGAAATCGGAGAAGGATTCTAGTTACTGGAGGAGCAGGCTTTATTGG
TGGAGCAGTTATAAGAAAGTTGCTTAAAGAGAGCACTTCTAAAATTTTCAATATCGACAAAATTGGATATGCAAGTGACC
TGACAGCTATAGACGAAATTTTAAGAACTAAAGATTATTCAGATCGTTATGATTTCGCAAAAATTGATCTTTCTATTCCA
GATGAAACAGCCAAAGCGATAAGTGATTCAGATCCAGACTTGATAATGCATTTGGCGGCAGAAAGCCATGTAGACAGATC
AATTCAAGGACCTGAGGCTTTCATAAATAGCAACATTTTTGGGACATTTAATCTTCTTGAAGCAACAAGAAAACACTATG
AAAATCTTTCGAATAAACGAAAAAATGACTTTCGTTTTCTACACATAAGCACTGATGAAGTATTTGGTTCCTTAGGTTTA
AATGGAAAATTTACAGAATCAACTTCCTATGACCCGAGAAGCCCATACTCCGCCAGCAAAGCATCAAGTGATCATTTAGT
TAGGTCATGGCACCACACCTTTCAACTTCCCATAGTCATAACAAATTGCAGTAATAACTTTGGACCATGGCAATTCCCTG
AAAAATTAATACCAGTAGCTATTAACAAAGCATTGGCTCTTAAATCCATACCACTTTATGGTGATGGAGAAAATATTAGA
GATTGGCTTTACGTCGATGATCACGTAGACGCTCTATTTCTTGCCGCTAATAAAGGTAAAATTGGAGATTCGTATTGTGT
AGGAGGGTATGGAGAGAGAAAAAATATAGAAATTTTAAAAATAATATGCAAAATTTTAGATGAAATATATCCTAAACATT
CACCCTTCGAACGACTTATTACAAAAGTACAGGACAGGAAAGGTCACGATCGAAGGTATGCAATCGATCCAAGCAAAATC
AGAAACGAATTGGGTTGGGAACCAAAGTACTCATTAGAAGATAGACTTGAAACGACAGTTCAATGGTACATAAAGAATAA
AAACTGGTCGCAAAGATTGATTCAAAGCTCGCCATAA

Upstream 100 bases:

>100_bases
AGACAATTTCTTAATCTTGAAGGCATTCATTGGAAAGAGAGCATCCTCAATGTGATGAAGCAAGTTTCAACTTGATAAAA
ACATATCAACATTCATAATT

Downstream 100 bases:

>100_bases
AATGGAGATTTGATTTGCAGCAAAACGATCACGAGTAAGAGGTAAATTTAAATTTATTTTAAAAACAAATGCAATACAGA
ATGTATATGGCGGGAGCCGT

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MEKNYFLNELLGNRRRILVTGGAGFIGGAVIRKLLKESTSKIFNIDKIGYASDLTAIDEILRTKDYSDRYDFAKIDLSIP
DETAKAISDSDPDLIMHLAAESHVDRSIQGPEAFINSNIFGTFNLLEATRKHYENLSNKRKNDFRFLHISTDEVFGSLGL
NGKFTESTSYDPRSPYSASKASSDHLVRSWHHTFQLPIVITNCSNNFGPWQFPEKLIPVAINKALALKSIPLYGDGENIR
DWLYVDDHVDALFLAANKGKIGDSYCVGGYGERKNIEILKIICKILDEIYPKHSPFERLITKVQDRKGHDRRYAIDPSKI
RNELGWEPKYSLEDRLETTVQWYIKNKNWSQRLIQSSP

Sequences:

>Translated_358_residues
MEKNYFLNELLGNRRRILVTGGAGFIGGAVIRKLLKESTSKIFNIDKIGYASDLTAIDEILRTKDYSDRYDFAKIDLSIP
DETAKAISDSDPDLIMHLAAESHVDRSIQGPEAFINSNIFGTFNLLEATRKHYENLSNKRKNDFRFLHISTDEVFGSLGL
NGKFTESTSYDPRSPYSASKASSDHLVRSWHHTFQLPIVITNCSNNFGPWQFPEKLIPVAINKALALKSIPLYGDGENIR
DWLYVDDHVDALFLAANKGKIGDSYCVGGYGERKNIEILKIICKILDEIYPKHSPFERLITKVQDRKGHDRRYAIDPSKI
RNELGWEPKYSLEDRLETTVQWYIKNKNWSQRLIQSSP
>Mature_358_residues
MEKNYFLNELLGNRRRILVTGGAGFIGGAVIRKLLKESTSKIFNIDKIGYASDLTAIDEILRTKDYSDRYDFAKIDLSIP
DETAKAISDSDPDLIMHLAAESHVDRSIQGPEAFINSNIFGTFNLLEATRKHYENLSNKRKNDFRFLHISTDEVFGSLGL
NGKFTESTSYDPRSPYSASKASSDHLVRSWHHTFQLPIVITNCSNNFGPWQFPEKLIPVAINKALALKSIPLYGDGENIR
DWLYVDDHVDALFLAANKGKIGDSYCVGGYGERKNIEILKIICKILDEIYPKHSPFERLITKVQDRKGHDRRYAIDPSKI
RNELGWEPKYSLEDRLETTVQWYIKNKNWSQRLIQSSP

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=32.0588235294118, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI42516563, Length=349, Percent_Identity=21.7765042979943, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=51.1494252873563, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=50.7288629737609, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=22.8571428571429, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=23.2091690544413, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=34.7058823529412, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=30.2670623145401, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=21.2827988338192, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=22.4089635854342, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40896; Mature: 40896

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNYFLNELLGNRRRILVTGGAGFIGGAVIRKLLKESTSKIFNIDKIGYASDLTAIDEI
CCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
LRTKDYSDRYDFAKIDLSIPDETAKAISDSDPDLIMHLAAESHVDRSIQGPEAFINSNIF
HHCCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHEEEHHHHHHHCCCCCCCHHHHCCCCC
GTFNLLEATRKHYENLSNKRKNDFRFLHISTDEVFGSLGLNGKFTESTSYDPRSPYSASK
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCEECCCCCCCCCCCCCCCC
ASSDHLVRSWHHTFQLPIVITNCSNNFGPWQFPEKLIPVAINKALALKSIPLYGDGENIR
CCCHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DWLYVDDHVDALFLAANKGKIGDSYCVGGYGERKNIEILKIICKILDEIYPKHSPFERLI
EEEEECCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
TKVQDRKGHDRRYAIDPSKIRNELGWEPKYSLEDRLETTVQWYIKNKNWSQRLIQSSP
HHHHHCCCCCCEEECCHHHHHHHCCCCCCCCHHHHHHHEEEEEEECCCHHHHHHCCCC
>Mature Secondary Structure
MEKNYFLNELLGNRRRILVTGGAGFIGGAVIRKLLKESTSKIFNIDKIGYASDLTAIDEI
CCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
LRTKDYSDRYDFAKIDLSIPDETAKAISDSDPDLIMHLAAESHVDRSIQGPEAFINSNIF
HHCCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHEEEHHHHHHHCCCCCCCHHHHCCCCC
GTFNLLEATRKHYENLSNKRKNDFRFLHISTDEVFGSLGLNGKFTESTSYDPRSPYSASK
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCEECCCCCCCCCCCCCCCC
ASSDHLVRSWHHTFQLPIVITNCSNNFGPWQFPEKLIPVAINKALALKSIPLYGDGENIR
CCCHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DWLYVDDHVDALFLAANKGKIGDSYCVGGYGERKNIEILKIICKILDEIYPKHSPFERLI
EEEEECCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
TKVQDRKGHDRRYAIDPSKIRNELGWEPKYSLEDRLETTVQWYIKNKNWSQRLIQSSP
HHHHHCCCCCCEEECCHHHHHHHCCCCCCCCHHHHHHHEEEEEEECCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]