Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 124025427

GI number: 124025427

Start: 663894

End: 665561

Strand: Direct

Name: prfC [H]

Synonym: NATL1_07201

Alternate gene names: 124025427

Gene position: 663894-665561 (Clockwise)

Preceding gene: 124025426

Following gene: 124025428

Centisome position: 35.6

GC content: 39.27

Gene sequence:

>1668_bases
ATGAAATCAACTCCCCAAGACAAGACTAATTTAGATCCAAATTTAGTAGAAGAATTAACTGTATCTTTAAGTAAAGAGGT
TAGTAAAAGAAGAAATTTTGCGATTATTTCCCACCCTGATGCAGGGAAAACTACTTTGACAGAAAAACTTTTACTTTATG
GCGGAGCTATTCAACAGGCAGGAGCAGTAAAAGCAAGAGGAGAACAGAGAAAAGTTACTTCTGATTGGATGGAGCTTGAA
AAACAACGAGGCATATCAATTACATCAACTGTTTTACAATTTGCTTATAAAGATAAGACGATAAATTTATTAGATACCCC
TGGCCACCAAGACTTCTCGGAAGATACGTATAGGACACTTGCCGCTGCTGATAATGCTGTAATGCTTGAGGATGCAGCAA
AAGGTCTTGAACCTCAAACAAGGAAGCTTTTTGAGGTTTGCAGAATGCGTCAGATACCAATTTTTACATTCATCAACAAG
ATGGATAGGCCAGGTCAAGAACCGCTGGAATTATTAGATGAGATTGAGTCTGAATTAGGGTTATTGACCTTTGCAGTTAA
TTGGCCAATAGGGAGTGGTGAGCTTTTTAGAGGTGTTGTTGAGCGTGCTACCAAAGAAGTTGTTCTATTTTCTAGAGCGG
AAAGAGGGAAGCAATCTAATGAAATAAGGTTAAAAATCAATGATCCTGAACTTAAAAACTTAGTCGAAGAAGAGCTATTG
ACAAAAGCACTTGAAGAGATTGAGATCCTAGATGAGGCTGGTTGTGACTTAAATCAAGAATTAATCTTGTCTGGCGAATT
AACTCCTGTGTTTTTTGGATCTGCAATGACCAACTTCGGGGTTAGGCCATTTTTAGATAATTTTCTTGATCTTTCTCAGG
GACCTGTCGCTCGAAATAGCTTTGATGGACCAATCGTTCCTACAAGAGAATCATTTAGTGGATTTGTATTTAAATTACAA
GCAAATATGGATCCAAAACATAGGGATAGAGTTGCTTTCGTGCGTGTATGTAGCGGGAGGTTTGAAAAAGATATGACAGT
TCAACACGCCAGGACAGGTAAACAAATCAGACTCTCAAGACCCCAAAAGATTTTCGGTCAAGATAGGGCTGTTGTTGATG
ATGCTTATCCTGGGGATGTTATTGGTTTGAATAATCCGGGGATGTTTTCTATTGGAGATACTTTATTTATTGGTCCGAGA
GTCGAATTCGAGGGCATCCCATGTTTTAGCCCCGAGATATTTAGTTGGTTAAGAAATCCAAATCCTTCAGCTTTTAAAAA
CTTTAGGAAAGGGGTCAATGAATTAAGAGAGGAGGGAGCAGTCCAAATTCTTTACGATAAAGATCAAAGCAAAAGAGATC
CAATTTTGGCCGCAGTTGGTCAGCTTCAGCTTGAGGTGGTACAGCATCGACTTGCTAGTGAATATGGGGTGGAAACTCGG
CTTGAACCAATGGGTTATCAAGTCGCCAGATGGGTAAAAGGGGGATGGCCTGCTTTGGACGAGGTTGGAAGAATTTTTAA
TTGCAAAACCGTTCAAGATGCTTGGCTTAGACCAGTACTGCTTTTTAAAAATGAATGGAATCTTAATCAGTTAAAGGAAG
ATCATCCTGAAATGGAATTAAACTCAGTTGCTCCGGTTGTTAGTGGTGTTGATCCTGTTTCTCTCTAA

Upstream 100 bases:

>100_bases
GTTATCTCAATTAGAGGTTTTTCAAGTTGATGAGCTTTATTTTGAGATTATTGGGTGTACTTAATCTTTCCCCTGAACAA
ATGCACAATCAAAAACAATA

Downstream 100 bases:

>100_bases
GGTAGATTAAAATCTAAAATTAAAGAGAGTTCATAAATCTTTTTTTGAAAAATCTATGGATCCTGGTAGCAATTCACAAT
CAAATTCAAACTCACAAGAT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKSTPQDKTNLDPNLVEELTVSLSKEVSKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELE
KQRGISITSTVLQFAYKDKTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRQIPIFTFINK
MDRPGQEPLELLDEIESELGLLTFAVNWPIGSGELFRGVVERATKEVVLFSRAERGKQSNEIRLKINDPELKNLVEEELL
TKALEEIEILDEAGCDLNQELILSGELTPVFFGSAMTNFGVRPFLDNFLDLSQGPVARNSFDGPIVPTRESFSGFVFKLQ
ANMDPKHRDRVAFVRVCSGRFEKDMTVQHARTGKQIRLSRPQKIFGQDRAVVDDAYPGDVIGLNNPGMFSIGDTLFIGPR
VEFEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDKDQSKRDPILAAVGQLQLEVVQHRLASEYGVETR
LEPMGYQVARWVKGGWPALDEVGRIFNCKTVQDAWLRPVLLFKNEWNLNQLKEDHPEMELNSVAPVVSGVDPVSL

Sequences:

>Translated_555_residues
MKSTPQDKTNLDPNLVEELTVSLSKEVSKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELE
KQRGISITSTVLQFAYKDKTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRQIPIFTFINK
MDRPGQEPLELLDEIESELGLLTFAVNWPIGSGELFRGVVERATKEVVLFSRAERGKQSNEIRLKINDPELKNLVEEELL
TKALEEIEILDEAGCDLNQELILSGELTPVFFGSAMTNFGVRPFLDNFLDLSQGPVARNSFDGPIVPTRESFSGFVFKLQ
ANMDPKHRDRVAFVRVCSGRFEKDMTVQHARTGKQIRLSRPQKIFGQDRAVVDDAYPGDVIGLNNPGMFSIGDTLFIGPR
VEFEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDKDQSKRDPILAAVGQLQLEVVQHRLASEYGVETR
LEPMGYQVARWVKGGWPALDEVGRIFNCKTVQDAWLRPVLLFKNEWNLNQLKEDHPEMELNSVAPVVSGVDPVSL
>Mature_555_residues
MKSTPQDKTNLDPNLVEELTVSLSKEVSKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGEQRKVTSDWMELE
KQRGISITSTVLQFAYKDKTINLLDTPGHQDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRQIPIFTFINK
MDRPGQEPLELLDEIESELGLLTFAVNWPIGSGELFRGVVERATKEVVLFSRAERGKQSNEIRLKINDPELKNLVEEELL
TKALEEIEILDEAGCDLNQELILSGELTPVFFGSAMTNFGVRPFLDNFLDLSQGPVARNSFDGPIVPTRESFSGFVFKLQ
ANMDPKHRDRVAFVRVCSGRFEKDMTVQHARTGKQIRLSRPQKIFGQDRAVVDDAYPGDVIGLNNPGMFSIGDTLFIGPR
VEFEGIPCFSPEIFSWLRNPNPSAFKNFRKGVNELREEGAVQILYDKDQSKRDPILAAVGQLQLEVVQHRLASEYGVETR
LEPMGYQVARWVKGGWPALDEVGRIFNCKTVQDAWLRPVLLFKNEWNLNQLKEDHPEMELNSVAPVVSGVDPVSL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=479, Percent_Identity=30.4801670146138, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI19923640, Length=580, Percent_Identity=25.3448275862069, Blast_Score=169, Evalue=8e-42,
Organism=Homo sapiens, GI25306287, Length=551, Percent_Identity=25.5898366606171, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI25306283, Length=408, Percent_Identity=28.6764705882353, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI157426893, Length=150, Percent_Identity=32.6666666666667, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI94966754, Length=200, Percent_Identity=31, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=34.2281879194631, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132016, Length=126, Percent_Identity=34.1269841269841, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310110807, Length=126, Percent_Identity=34.1269841269841, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310123363, Length=126, Percent_Identity=34.1269841269841, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=471, Percent_Identity=50.3184713375796, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789738, Length=497, Percent_Identity=29.5774647887324, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=38.255033557047, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=33.879781420765, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789737, Length=150, Percent_Identity=30.6666666666667, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=150, Percent_Identity=30.6666666666667, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=490, Percent_Identity=27.3469387755102, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=480, Percent_Identity=26.875, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17557151, Length=151, Percent_Identity=33.7748344370861, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.0746268656716, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.6770186335404, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=508, Percent_Identity=29.5275590551181, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322359, Length=524, Percent_Identity=25.9541984732824, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=33.7748344370861, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=34.1772151898734, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=34.1772151898734, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582462, Length=495, Percent_Identity=28.8888888888889, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI221458488, Length=504, Percent_Identity=25, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI78706572, Length=184, Percent_Identity=32.6086956521739, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28574573, Length=152, Percent_Identity=33.5526315789474, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 62454; Mature: 62454

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTPQDKTNLDPNLVEELTVSLSKEVSKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCHHHHC
GAVKARGEQRKVTSDWMELEKQRGISITSTVLQFAYKDKTINLLDTPGHQDFSEDTYRTL
CCHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
AAADNAVMLEDAAKGLEPQTRKLFEVCRMRQIPIFTFINKMDRPGQEPLELLDEIESELG
HHCCCEEEEHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LLTFAVNWPIGSGELFRGVVERATKEVVLFSRAERGKQSNEIRLKINDPELKNLVEEELL
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
TKALEEIEILDEAGCDLNQELILSGELTPVFFGSAMTNFGVRPFLDNFLDLSQGPVARNS
HHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECHHHHHCCCHHHHHHHHCCCCCCCCCCC
FDGPIVPTRESFSGFVFKLQANMDPKHRDRVAFVRVCSGRFEKDMTVQHARTGKQIRLSR
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCC
PQKIFGQDRAVVDDAYPGDVIGLNNPGMFSIGDTLFIGPRVEFEGIPCFSPEIFSWLRNP
CHHHHCCCCCEECCCCCCCEEECCCCCCEECCCEEEECCEEEECCCCCCCHHHHHHHCCC
NPSAFKNFRKGVNELREEGAVQILYDKDQSKRDPILAAVGQLQLEVVQHRLASEYGVETR
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEPMGYQVARWVKGGWPALDEVGRIFNCKTVQDAWLRPVLLFKNEWNLNQLKEDHPEMEL
CCCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHCCEEEEEECCCCHHHHHHCCCCCCH
NSVAPVVSGVDPVSL
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSTPQDKTNLDPNLVEELTVSLSKEVSKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCHHHHC
GAVKARGEQRKVTSDWMELEKQRGISITSTVLQFAYKDKTINLLDTPGHQDFSEDTYRTL
CCHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
AAADNAVMLEDAAKGLEPQTRKLFEVCRMRQIPIFTFINKMDRPGQEPLELLDEIESELG
HHCCCEEEEHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LLTFAVNWPIGSGELFRGVVERATKEVVLFSRAERGKQSNEIRLKINDPELKNLVEEELL
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
TKALEEIEILDEAGCDLNQELILSGELTPVFFGSAMTNFGVRPFLDNFLDLSQGPVARNS
HHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECHHHHHCCCHHHHHHHHCCCCCCCCCCC
FDGPIVPTRESFSGFVFKLQANMDPKHRDRVAFVRVCSGRFEKDMTVQHARTGKQIRLSR
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCC
PQKIFGQDRAVVDDAYPGDVIGLNNPGMFSIGDTLFIGPRVEFEGIPCFSPEIFSWLRNP
CHHHHCCCCCEECCCCCCCEEECCCCCCEECCCEEEECCEEEECCCCCCCHHHHHHHCCC
NPSAFKNFRKGVNELREEGAVQILYDKDQSKRDPILAAVGQLQLEVVQHRLASEYGVETR
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LEPMGYQVARWVKGGWPALDEVGRIFNCKTVQDAWLRPVLLFKNEWNLNQLKEDHPEMEL
CCCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHCCEEEEEECCCCHHHHHHCCCCCCH
NSVAPVVSGVDPVSL
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA