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The map label for this gene is mccB [H]

Identifier: 124025172

GI number: 124025172

Start: 420825

End: 422291

Strand: Reverse

Name: mccB [H]

Synonym: NATL1_04591

Alternate gene names: 124025172

Gene position: 422291-420825 (Counterclockwise)

Preceding gene: 124025173

Following gene: 124025171

Centisome position: 22.65

GC content: 35.58

Gene sequence:

>1467_bases
TTGACTGAAAGAAATTTACTTATTGATCCTTGCTGGAGTGCCAAAGATTTAGGAGAGCCTCTACCAAGTAGGCCACATGC
TGTATCTGTTGCATTGCCTAGATGGGAAGATGTAATCGCATACGAAGAGAAGGTTCCAGCTTGTATAAATTCATTAAAAG
CAATTTACCCTAGATTTGGATTTAATCCTTTTGTTGCAGAGGTAGCTCGAAAATCACTTGAATTTCATGGCGAATCGAAC
AAAAGCAGTTGGCCTTACCCCAACAGAGCTTCTGCTTTAAGTGCCCAGGAATATTGTCTTCGAAAGAATTCAGATTGTTT
TTCAAAAATAGAAGACTTTCTTGGCGTTTCGTGTCTAATTATTGACCAAAAAAATACCTCTTCAGCAAAAGCCTTCTGGC
AACACACTGGTCTTGGATTGTCTTCACGTGAAGCGGCAATAGCCCTTGGGAAAGAGAAGAAACCACTCACTTCAGATGGT
GATTACGCCAGAGAGGAACTTATCAAACGCTTGGCAAATATATACAATTGCGATAAGTGTCTAATACGACTACATCGTTC
AGGTATGGCTGCCCTAACGACTATTATTTCAGCTATAAATTGTATCAAAGGGACTTCTTCGACTCTTCAAATAGGCTTCC
CATATGTTGATGTTCTTAAACTCCCTCAAATAATTTTCCAAGGAAGCGATCTCATTATTAAAACAAAATTAAATCATATA
AAAGAAGAGCTGGATAAAAAAAATCCAGCTGCATTAATCATAGAAATACCTAGTAACCCTTTATTGCAATGTGTTGACCT
AATATCTATTTCAAAATTAGCTAAAGAAAAAAACATACCTGTCATTGTTGACGATACCATCGGTTCATCTCTAAATGTTC
ACCTAACTCCTTATGCAGATGTGATTTTTAGCTCTCTTACAAAAAGTTTTGCTGGGAGAGGAGATATTCTTGCTGGCAGT
ACTGTTATCAGTCCATACTCTAAATGGAAAAAAGAAATAGCTGAGATAATTCCTAAAGTTGCATTATCTACACTATCTGA
TTCAGATGCTATTGAGTTGGAGTTAACTAGTAGAGACGTAAAGGATCGCTTAAAAAGGTTAAATCAATCTTGCTTAAAGC
TGAAAGAAAAATTAGAAACAAAAAAAGAAATTTCAAAGGTTTTACATCCTCAATATTGTAAAAATTTTAATTCGCTATTA
AAAAAAGGAGGAGGTTATGGATGTCTATTATCGTTTGAACTTAAAGGAGGGATTGAAGAATCAAAAAGAGTTTATGATTC
ATTAAGAGTAAACAAAGGACCAAGCTTAGGTACAAATTTTACATTGGTTTGTCCATATGTTCAGTTAGCTCACTTCAAAG
AATTGAAATGGGCTGAGAGTTGTGGCGTCTCGGAACACTTATTAAGAGTGTCAGTAGGGCTAGAAAATGAAGACGAATTA
TGGTCAAGATTTAATTCAGTAATATAA

Upstream 100 bases:

>100_bases
TAAAAATTGGAATTACTGATTCACTTATTCGGTTTTCTATTGGATGTGAGGACATTGAAGACTTAAGTGCTGATTTGAAT
CAAGCCTTAGGAACTATCTC

Downstream 100 bases:

>100_bases
AAAAATCACTAATAAAGATTATCTAAACAACCAATTACCGAGATTTTTATATAGATTTAGAGTATTAGTTAATTAAGTCA
TTTAAAAAATGTCCAGAATC

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTERNLLIDPCWSAKDLGEPLPSRPHAVSVALPRWEDVIAYEEKVPACINSLKAIYPRFGFNPFVAEVARKSLEFHGESN
KSSWPYPNRASALSAQEYCLRKNSDCFSKIEDFLGVSCLIIDQKNTSSAKAFWQHTGLGLSSREAAIALGKEKKPLTSDG
DYAREELIKRLANIYNCDKCLIRLHRSGMAALTTIISAINCIKGTSSTLQIGFPYVDVLKLPQIIFQGSDLIIKTKLNHI
KEELDKKNPAALIIEIPSNPLLQCVDLISISKLAKEKNIPVIVDDTIGSSLNVHLTPYADVIFSSLTKSFAGRGDILAGS
TVISPYSKWKKEIAEIIPKVALSTLSDSDAIELELTSRDVKDRLKRLNQSCLKLKEKLETKKEISKVLHPQYCKNFNSLL
KKGGGYGCLLSFELKGGIEESKRVYDSLRVNKGPSLGTNFTLVCPYVQLAHFKELKWAESCGVSEHLLRVSVGLENEDEL
WSRFNSVI

Sequences:

>Translated_488_residues
MTERNLLIDPCWSAKDLGEPLPSRPHAVSVALPRWEDVIAYEEKVPACINSLKAIYPRFGFNPFVAEVARKSLEFHGESN
KSSWPYPNRASALSAQEYCLRKNSDCFSKIEDFLGVSCLIIDQKNTSSAKAFWQHTGLGLSSREAAIALGKEKKPLTSDG
DYAREELIKRLANIYNCDKCLIRLHRSGMAALTTIISAINCIKGTSSTLQIGFPYVDVLKLPQIIFQGSDLIIKTKLNHI
KEELDKKNPAALIIEIPSNPLLQCVDLISISKLAKEKNIPVIVDDTIGSSLNVHLTPYADVIFSSLTKSFAGRGDILAGS
TVISPYSKWKKEIAEIIPKVALSTLSDSDAIELELTSRDVKDRLKRLNQSCLKLKEKLETKKEISKVLHPQYCKNFNSLL
KKGGGYGCLLSFELKGGIEESKRVYDSLRVNKGPSLGTNFTLVCPYVQLAHFKELKWAESCGVSEHLLRVSVGLENEDEL
WSRFNSVI
>Mature_487_residues
TERNLLIDPCWSAKDLGEPLPSRPHAVSVALPRWEDVIAYEEKVPACINSLKAIYPRFGFNPFVAEVARKSLEFHGESNK
SSWPYPNRASALSAQEYCLRKNSDCFSKIEDFLGVSCLIIDQKNTSSAKAFWQHTGLGLSSREAAIALGKEKKPLTSDGD
YAREELIKRLANIYNCDKCLIRLHRSGMAALTTIISAINCIKGTSSTLQIGFPYVDVLKLPQIIFQGSDLIIKTKLNHIK
EELDKKNPAALIIEIPSNPLLQCVDLISISKLAKEKNIPVIVDDTIGSSLNVHLTPYADVIFSSLTKSFAGRGDILAGST
VISPYSKWKKEIAEIIPKVALSTLSDSDAIELELTSRDVKDRLKRLNQSCLKLKEKLETKKEISKVLHPQYCKNFNSLLK
KGGGYGCLLSFELKGGIEESKRVYDSLRVNKGPSLGTNFTLVCPYVQLAHFKELKWAESCGVSEHLLRVSVGLENEDELW
SRFNSVI

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=245, Percent_Identity=28.1632653061224, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71980708, Length=258, Percent_Identity=26.3565891472868, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538055, Length=346, Percent_Identity=25.4335260115607, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560056, Length=337, Percent_Identity=24.9258160237389, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=573, Percent_Identity=29.3193717277487, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6322590, Length=303, Percent_Identity=36.6336633663366, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323557, Length=350, Percent_Identity=33.1428571428571, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6319307, Length=263, Percent_Identity=29.6577946768061, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19922554, Length=258, Percent_Identity=27.906976744186, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1; =4.4.1.2 [H]

Molecular weight: Translated: 54291; Mature: 54160

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERNLLIDPCWSAKDLGEPLPSRPHAVSVALPRWEDVIAYEEKVPACINSLKAIYPRFG
CCCCCEEECCCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
FNPFVAEVARKSLEFHGESNKSSWPYPNRASALSAQEYCLRKNSDCFSKIEDFLGVSCLI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEE
IDQKNTSSAKAFWQHTGLGLSSREAAIALGKEKKPLTSDGDYAREELIKRLANIYNCDKC
EECCCCCHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIRLHRSGMAALTTIISAINCIKGTSSTLQIGFPYVDVLKLPQIIFQGSDLIIKTKLNHI
HHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHH
KEELDKKNPAALIIEIPSNPLLQCVDLISISKLAKEKNIPVIVDDTIGSSLNVHLTPYAD
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECHHHH
VIFSSLTKSFAGRGDILAGSTVISPYSKWKKEIAEIIPKVALSTLSDSDAIELELTSRDV
HHHHHHHHHHCCCCCEEECCHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHH
KDRLKRLNQSCLKLKEKLETKKEISKVLHPQYCKNFNSLLKKGGGYGCLLSFELKGGIEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHH
SKRVYDSLRVNKGPSLGTNFTLVCPYVQLAHFKELKWAESCGVSEHLLRVSVGLENEDEL
HHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHH
WSRFNSVI
HHHHHCCC
>Mature Secondary Structure 
TERNLLIDPCWSAKDLGEPLPSRPHAVSVALPRWEDVIAYEEKVPACINSLKAIYPRFG
CCCCEEECCCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
FNPFVAEVARKSLEFHGESNKSSWPYPNRASALSAQEYCLRKNSDCFSKIEDFLGVSCLI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEE
IDQKNTSSAKAFWQHTGLGLSSREAAIALGKEKKPLTSDGDYAREELIKRLANIYNCDKC
EECCCCCHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIRLHRSGMAALTTIISAINCIKGTSSTLQIGFPYVDVLKLPQIIFQGSDLIIKTKLNHI
HHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHH
KEELDKKNPAALIIEIPSNPLLQCVDLISISKLAKEKNIPVIVDDTIGSSLNVHLTPYAD
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECHHHH
VIFSSLTKSFAGRGDILAGSTVISPYSKWKKEIAEIIPKVALSTLSDSDAIELELTSRDV
HHHHHHHHHHCCCCCEEECCHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHH
KDRLKRLNQSCLKLKEKLETKKEISKVLHPQYCKNFNSLLKKGGGYGCLLSFELKGGIEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHH
SKRVYDSLRVNKGPSLGTNFTLVCPYVQLAHFKELKWAESCGVSEHLLRVSVGLENEDEL
HHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHH
WSRFNSVI
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]