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The map label for this gene is metB [H]

Identifier: 124025173

GI number: 124025173

Start: 422288

End: 423457

Strand: Reverse

Name: metB [H]

Synonym: NATL1_04601

Alternate gene names: 124025173

Gene position: 423457-422288 (Counterclockwise)

Preceding gene: 124025174

Following gene: 124025172

Centisome position: 22.71

GC content: 37.95

Gene sequence:

>1170_bases
ATGGGATCAGTAAAAAGAGACAAGAAGTTCAGCAATGGAGTAAACACAAGAGTCATTCATCACAAAGATAATTTTTCTGA
AGGAACTGGTTCAATAATGCCGCCAATCTTTCCAACCTCAACGTTCGTTCATGGCAATGAGGGTGGCTTTGATTACACTC
GTTCAGGAAATCCAAATTTTCGAATTCTTGAATCAGTTTTGTCTGATCTGGAAGAGTGTCAATTCGCTAGTGTATTTAGT
TCTGGAGTCGCTGCGATTACAGCAATAGTCTCCACTCTTCAGGCTGGAGACTTAATCCTTTGTGAGGAGAACCTTTATGG
ATGTACGGTGAGATTATTTGAACAAGTTTTTAATCGTTTTGGATTAAAAACTCAATGGATAGACTTTACTAAGCCCAATT
TCCAAGAAGTCATTTCAAATCACAAACCCGCGATGATTTGGATCGAAAGTCCTACTAACCCACTCCTCAAAATTATTGAT
ATTGAAGGGATTTGTCATTTCTCAAATAAAATGAAAATACCTGTTGTTGTAGACAATACTTTTGCAACACCTCTATTACA
AAGACCTCTTAAACTTGGAGCGACCTTATCTTTAACTAGCACGACCAAGTTTATTAATGGTCACTCAGATGCACTTGGAG
GTGCAGTATGCACCGAGAATCCTATCTGGAGAGACAAGCTAAATTTCGCCCAGAAAGCTCTTGGATTAAACCCTTCTCCC
TTTGATTGCTGGCTTATCACACGAGGAATAAAAACTCTTCCACTTCGCCTAGAAAGACAAGTTAATAATGCATCCAAAAT
AGCTAATCAATTAGCCGATAATCCAGCAATAAAATATGTTCGATATCCTTTCAGGAATGATCACCCACAATGTAAATTAG
CAAAAAAACAAATGGCTATGGGAGGAGCAATTGTTACTGCCACTGTTAACGCAACCCAAGCTCAAACCTATTCATTTTGT
AAAAGTCTTCATTACTTCAAAATGGCAGAAAGTCTGGGAGGAATTGAAAGTCTTGTTTGCCATCCAGCTACAATGACACA
TGCTTCAGTGTCCAAGGAAACAAAATTAAAAATTGGAATTACTGATTCACTTATTCGGTTTTCTATTGGATGTGAGGACA
TTGAAGACTTAAGTGCTGATTTGAATCAAGCCTTAGGAACTATCTCTTGA

Upstream 100 bases:

>100_bases
CACACATAGCTTCTTTCTCATTAAATTTAATTATGGTGCCCCTTTTTTTAGGGGCTTTTTCATGTTTTTCTATAGCCTAT
AGAAAAGCCACTCAAAGGAT

Downstream 100 bases:

>100_bases
CTGAAAGAAATTTACTTATTGATCCTTGCTGGAGTGCCAAAGATTTAGGAGAGCCTCTACCAAGTAGGCCACATGCTGTA
TCTGTTGCATTGCCTAGATG

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MGSVKRDKKFSNGVNTRVIHHKDNFSEGTGSIMPPIFPTSTFVHGNEGGFDYTRSGNPNFRILESVLSDLEECQFASVFS
SGVAAITAIVSTLQAGDLILCEENLYGCTVRLFEQVFNRFGLKTQWIDFTKPNFQEVISNHKPAMIWIESPTNPLLKIID
IEGICHFSNKMKIPVVVDNTFATPLLQRPLKLGATLSLTSTTKFINGHSDALGGAVCTENPIWRDKLNFAQKALGLNPSP
FDCWLITRGIKTLPLRLERQVNNASKIANQLADNPAIKYVRYPFRNDHPQCKLAKKQMAMGGAIVTATVNATQAQTYSFC
KSLHYFKMAESLGGIESLVCHPATMTHASVSKETKLKIGITDSLIRFSIGCEDIEDLSADLNQALGTIS

Sequences:

>Translated_389_residues
MGSVKRDKKFSNGVNTRVIHHKDNFSEGTGSIMPPIFPTSTFVHGNEGGFDYTRSGNPNFRILESVLSDLEECQFASVFS
SGVAAITAIVSTLQAGDLILCEENLYGCTVRLFEQVFNRFGLKTQWIDFTKPNFQEVISNHKPAMIWIESPTNPLLKIID
IEGICHFSNKMKIPVVVDNTFATPLLQRPLKLGATLSLTSTTKFINGHSDALGGAVCTENPIWRDKLNFAQKALGLNPSP
FDCWLITRGIKTLPLRLERQVNNASKIANQLADNPAIKYVRYPFRNDHPQCKLAKKQMAMGGAIVTATVNATQAQTYSFC
KSLHYFKMAESLGGIESLVCHPATMTHASVSKETKLKIGITDSLIRFSIGCEDIEDLSADLNQALGTIS
>Mature_388_residues
GSVKRDKKFSNGVNTRVIHHKDNFSEGTGSIMPPIFPTSTFVHGNEGGFDYTRSGNPNFRILESVLSDLEECQFASVFSS
GVAAITAIVSTLQAGDLILCEENLYGCTVRLFEQVFNRFGLKTQWIDFTKPNFQEVISNHKPAMIWIESPTNPLLKIIDI
EGICHFSNKMKIPVVVDNTFATPLLQRPLKLGATLSLTSTTKFINGHSDALGGAVCTENPIWRDKLNFAQKALGLNPSPF
DCWLITRGIKTLPLRLERQVNNASKIANQLADNPAIKYVRYPFRNDHPQCKLAKKQMAMGGAIVTATVNATQAQTYSFCK
SLHYFKMAESLGGIESLVCHPATMTHASVSKETKLKIGITDSLIRFSIGCEDIEDLSADLNQALGTIS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=375, Percent_Identity=43.7333333333333, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI299473759, Length=375, Percent_Identity=40.2666666666667, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI25453487, Length=374, Percent_Identity=37.9679144385027, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1790375, Length=365, Percent_Identity=36.1643835616438, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1789383, Length=350, Percent_Identity=24.5714285714286, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17538055, Length=363, Percent_Identity=43.801652892562, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI17560056, Length=360, Percent_Identity=41.9444444444444, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=34.2391304347826, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=37.5647668393782, Blast_Score=255, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6321254, Length=383, Percent_Identity=31.5926892950392, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=411, Percent_Identity=31.1435523114355, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=28.7179487179487, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=285, Percent_Identity=28.0701754385965, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=40.5759162303665, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 42866; Mature: 42734

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVKRDKKFSNGVNTRVIHHKDNFSEGTGSIMPPIFPTSTFVHGNEGGFDYTRSGNPNF
CCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCH
RILESVLSDLEECQFASVFSSGVAAITAIVSTLQAGDLILCEENLYGCTVRLFEQVFNRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
GLKTQWIDFTKPNFQEVISNHKPAMIWIESPTNPLLKIIDIEGICHFSNKMKIPVVVDNT
CCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCEEEEEEECCEEECCCCEEEEEEECCC
FATPLLQRPLKLGATLSLTSTTKFINGHSDALGGAVCTENPIWRDKLNFAQKALGLNPSP
CCCHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCC
FDCWLITRGIKTLPLRLERQVNNASKIANQLADNPAIKYVRYPFRNDHPQCKLAKKQMAM
CEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHC
GGAIVTATVNATQAQTYSFCKSLHYFKMAESLGGIESLVCHPATMTHASVSKETKLKIGI
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCCCEEEEEC
TDSLIRFSIGCEDIEDLSADLNQALGTIS
CHHHHHEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSVKRDKKFSNGVNTRVIHHKDNFSEGTGSIMPPIFPTSTFVHGNEGGFDYTRSGNPNF
CCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCH
RILESVLSDLEECQFASVFSSGVAAITAIVSTLQAGDLILCEENLYGCTVRLFEQVFNRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
GLKTQWIDFTKPNFQEVISNHKPAMIWIESPTNPLLKIIDIEGICHFSNKMKIPVVVDNT
CCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCEEEEEEECCEEECCCCEEEEEEECCC
FATPLLQRPLKLGATLSLTSTTKFINGHSDALGGAVCTENPIWRDKLNFAQKALGLNPSP
CCCHHHHHHHHCCCEEEECCHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCC
FDCWLITRGIKTLPLRLERQVNNASKIANQLADNPAIKYVRYPFRNDHPQCKLAKKQMAM
CEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHC
GGAIVTATVNATQAQTYSFCKSLHYFKMAESLGGIESLVCHPATMTHASVSKETKLKIGI
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCCCEEEEEC
TDSLIRFSIGCEDIEDLSADLNQALGTIS
CHHHHHEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]