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The map label for this gene is pgk [H]

Identifier: 124027813

GI number: 124027813

Start: 946984

End: 948267

Strand: Reverse

Name: pgk [H]

Synonym: Hbut_0940

Alternate gene names: 124027813

Gene position: 948267-946984 (Counterclockwise)

Preceding gene: 124027814

Following gene: 124027812

Centisome position: 56.88

GC content: 52.1

Gene sequence:

>1284_bases
TTGCCGTGCCTTCTCCGTAATGGAGAGGTTATACCCACCATTGACGACCTTGACGAGAATTATGGTGTAAAGGGTTCTAA
AATACTTGTGAGAATCGATATTAACAGCCCCATAGATCCCAGGACTGGCCGCATACTCGACGACACACGTATCAGAGCTC
ATGCCAAGACTATCAGAGAGCTTTCTGAACGCGGCGCAGCCGTAGTACTAATCTCGCACCAGGGCAGACCCGGTGAGCCA
GACTTTACGAGCCTAGAGCCACACAGCAAGGTTCTCTCAGACCGGCTCGGCATGACGGTAAAGTTTGTTGATGACGTTAT
CGGCCCCGCAGCAAGGCAGGCAATAAAGGAGCTTAAACCCGGCGAGATACTACTCTTGGATAACACGAGACTCGTATCGG
AGGAGATCATAGAGGCTACACCGGAAAAGCATGCCGGAAGCATCTTCGTGCAAAGGCTAGCCCCCCTCTTCAACTACTAT
ATAAACGACGCATTTGCAACCGCCCACCGCAGCCAGCCGAGCATCGTCGGATTCCCAATGCTACTCCCATCAGCTGCTGG
CAGAGTTCTCGAGAGAGAGCTGCACGCGCTATCAAAACTCTACAAGCCAGAGGAGAGGCCGAGAGTATTTGTCCTGGGAG
GAGGTAAGGTCCACGATACGCTTAGGATACTGGAGTACTTGCACGCAAACCGAGCCGCTGACCGCATTCTTGCAACAGGC
CTCGTAGCAGAACTGTTCATGGTTGCCAAGGGCATAAACATCGGTAGGAAAAACATTGAAGTCCTTGAGGCTAAGGGTAT
TCTAGCACTAGTTCCCAGGGCACGCAGGCTACTGCTGAGAGGCCTTCCCCTTGAAACACCAGTGGACTTCGTAACAGAGC
ATAATGGCGAGGTACGTGTAGAAGCTGTTGGCACAGGCATAGAAGGTGTAATAAGAGACATAGGGCCTCAGACCATCTCA
ATGTACTCCGAGATGATGAAGGAAGCTAGGCTAATAGTACTCAGAGGCCCCGCTGGCGTCATAGAGGATCCAAGATTTAG
GAAAGGCACCGAAGAACTGGTGAAATCTGCAGCAGAGTCAAACGCCTATGTAATAATCGGCGGCGGCCACCTCAACGTAA
TAGCCTCCAACCTAGGGCTAGCCGATAAGGAAAACATGCACCTAAGTACTGGCGGTGGAGCACTCCTCTTATTCCTCGCA
GGCGAAGACCTACCCGGCCTCACAGCCCTCGCAAAATCTGCTCGTAAGTTCTTCCCCCAGCTCTCAGCCAAACACTCCAC
ATAA

Upstream 100 bases:

>100_bases
GGTATCTCCTCAACGCCCGTTAGCAGCTGTTTTAACTTATGCGCGGCTCTTTTAGCTTCCCCCGGATGCAGTATACTACC
CTCCCCACTCGGGAGCGCTT

Downstream 100 bases:

>100_bases
ACCACTACAAGCTCTTCCCCCTGAGAGGTGAAGAGCCGTGGCACGGGTTAGGGTTGGCGTAAACGGGTTTGGAACTATAG
GTAAGCGTGTAGCTGAGGCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPCLLRNGEVIPTIDDLDENYGVKGSKILVRIDINSPIDPRTGRILDDTRIRAHAKTIRELSERGAAVVLISHQGRPGEP
DFTSLEPHSKVLSDRLGMTVKFVDDVIGPAARQAIKELKPGEILLLDNTRLVSEEIIEATPEKHAGSIFVQRLAPLFNYY
INDAFATAHRSQPSIVGFPMLLPSAAGRVLERELHALSKLYKPEERPRVFVLGGGKVHDTLRILEYLHANRAADRILATG
LVAELFMVAKGINIGRKNIEVLEAKGILALVPRARRLLLRGLPLETPVDFVTEHNGEVRVEAVGTGIEGVIRDIGPQTIS
MYSEMMKEARLIVLRGPAGVIEDPRFRKGTEELVKSAAESNAYVIIGGGHLNVIASNLGLADKENMHLSTGGGALLLFLA
GEDLPGLTALAKSARKFFPQLSAKHST

Sequences:

>Translated_427_residues
MPCLLRNGEVIPTIDDLDENYGVKGSKILVRIDINSPIDPRTGRILDDTRIRAHAKTIRELSERGAAVVLISHQGRPGEP
DFTSLEPHSKVLSDRLGMTVKFVDDVIGPAARQAIKELKPGEILLLDNTRLVSEEIIEATPEKHAGSIFVQRLAPLFNYY
INDAFATAHRSQPSIVGFPMLLPSAAGRVLERELHALSKLYKPEERPRVFVLGGGKVHDTLRILEYLHANRAADRILATG
LVAELFMVAKGINIGRKNIEVLEAKGILALVPRARRLLLRGLPLETPVDFVTEHNGEVRVEAVGTGIEGVIRDIGPQTIS
MYSEMMKEARLIVLRGPAGVIEDPRFRKGTEELVKSAAESNAYVIIGGGHLNVIASNLGLADKENMHLSTGGGALLLFLA
GEDLPGLTALAKSARKFFPQLSAKHST
>Mature_426_residues
PCLLRNGEVIPTIDDLDENYGVKGSKILVRIDINSPIDPRTGRILDDTRIRAHAKTIRELSERGAAVVLISHQGRPGEPD
FTSLEPHSKVLSDRLGMTVKFVDDVIGPAARQAIKELKPGEILLLDNTRLVSEEIIEATPEKHAGSIFVQRLAPLFNYYI
NDAFATAHRSQPSIVGFPMLLPSAAGRVLERELHALSKLYKPEERPRVFVLGGGKVHDTLRILEYLHANRAADRILATGL
VAELFMVAKGINIGRKNIEVLEAKGILALVPRARRLLLRGLPLETPVDFVTEHNGEVRVEAVGTGIEGVIRDIGPQTISM
YSEMMKEARLIVLRGPAGVIEDPRFRKGTEELVKSAAESNAYVIIGGGHLNVIASNLGLADKENMHLSTGGGALLLFLAG
EDLPGLTALAKSARKFFPQLSAKHST

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=422, Percent_Identity=33.6492890995261, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI31543397, Length=424, Percent_Identity=33.7264150943396, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1789294, Length=409, Percent_Identity=33.7408312958435, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17508823, Length=408, Percent_Identity=34.5588235294118, Blast_Score=185, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6319857, Length=425, Percent_Identity=34.1176470588235, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17136394, Length=419, Percent_Identity=34.3675417661098, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=31.6546762589928, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 46560; Mature: 46429

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCLLRNGEVIPTIDDLDENYGVKGSKILVRIDINSPIDPRTGRILDDTRIRAHAKTIRE
CCCEECCCCCCCCHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHH
LSERGAAVVLISHQGRPGEPDFTSLEPHSKVLSDRLGMTVKFVDDVIGPAARQAIKELKP
HHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCC
GEILLLDNTRLVSEEIIEATPEKHAGSIFVQRLAPLFNYYINDAFATAHRSQPSIVGFPM
CCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHH
LLPSAAGRVLERELHALSKLYKPEERPRVFVLGGGKVHDTLRILEYLHANRAADRILATG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LVAELFMVAKGINIGRKNIEVLEAKGILALVPRARRLLLRGLPLETPVDFVTEHNGEVRV
HHHHHHHHHCCCCCCCCCEEEEECCCHHEECHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
EAVGTGIEGVIRDIGPQTISMYSEMMKEARLIVLRGPAGVIEDPRFRKGTEELVKSAAES
EEECCCHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCC
NAYVIIGGGHLNVIASNLGLADKENMHLSTGGGALLLFLAGEDLPGLTALAKSARKFFPQ
CCEEEEECCEEEEEECCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
LSAKHST
HCCCCCC
>Mature Secondary Structure 
PCLLRNGEVIPTIDDLDENYGVKGSKILVRIDINSPIDPRTGRILDDTRIRAHAKTIRE
CCEECCCCCCCCHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHH
LSERGAAVVLISHQGRPGEPDFTSLEPHSKVLSDRLGMTVKFVDDVIGPAARQAIKELKP
HHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCC
GEILLLDNTRLVSEEIIEATPEKHAGSIFVQRLAPLFNYYINDAFATAHRSQPSIVGFPM
CCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHH
LLPSAAGRVLERELHALSKLYKPEERPRVFVLGGGKVHDTLRILEYLHANRAADRILATG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LVAELFMVAKGINIGRKNIEVLEAKGILALVPRARRLLLRGLPLETPVDFVTEHNGEVRV
HHHHHHHHHCCCCCCCCCEEEEECCCHHEECHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
EAVGTGIEGVIRDIGPQTISMYSEMMKEARLIVLRGPAGVIEDPRFRKGTEELVKSAAES
EEECCCHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCC
NAYVIIGGGHLNVIASNLGLADKENMHLSTGGGALLLFLAGEDLPGLTALAKSARKFFPQ
CCEEEEECCEEEEEECCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
LSAKHST
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA