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The map label for this gene is gatB [H]

Identifier: 124027709

GI number: 124027709

Start: 829289

End: 830734

Strand: Reverse

Name: gatB [H]

Synonym: Hbut_0832

Alternate gene names: 124027709

Gene position: 830734-829289 (Counterclockwise)

Preceding gene: 124027710

Following gene: 124027705

Centisome position: 49.83

GC content: 54.01

Gene sequence:

>1446_bases
ATGGCTGTAAGCGGTGTCAAGGCGGTTATAGGGCTTGAAGTCCACGTACAAATAACAAGTCTCAAGACCAAGTTATTCTG
TAGCTGTAGCGCTGACTACCGTGGCGCCCCGCCAAACACCCATGTCTGTCCTGTCTGCCTCGGTCTCCCCGGCGCTCTCC
CAGTAGTCAACGAGGAGGCGGTCAAGAAGGCGATACAACTCTGCCTAGCAATAAACGGCGAGGTAGCCAACATTCTCCGG
TTCGACAGGAAGCACTACTTCTACCCCGACCTTCCCAAAAACTACCAGATAACACAGTACCTCGAGCCCATATGCAGGGG
CGGCTATATCGAGATAGAGACGGGCAAGGGGCCCAAGAAGATTAGGATACGGAGGATAAACCTGGAGGAAGATCCTGGCA
GGATTGTCTACCCCGGCGGTAGCCCACTAGCAAGCCCCTATGCGCTAGTGGACTACAATAGGAGCGGCGTAGCACTTCTC
GAAATAGTAACCGAGCCAGATATGGAGTCCCCCGAGGAGGCTGTAGCCTTTCTCGAAAAACTCCACAGCATAGTAGAGCA
TCTCGGCATTTGTGACTGCAGGCTAGAAGGCTCAATCCGCGTGGACGCTAACGTAAGCATTGAAGGTGGAGAAAGGGTCG
AGGTAAAGAATATTGGTGGTTTCAGCGAGGTGAAGAAGGCCCTCTACTACGAGATTACAAGGCAACGCGACCTCCTAATG
AAGGGCCGGAATGTGAAGCGTGAGACCCGCCACTGGGACCCGGTGCGCAAGGTGACGTTGCCAGCTCGTGTTAAGGAGAC
GGAGGAGGACTACAGATACATGCCTGACCCCAACCTGCCGCCAGTAAGGATAAGTGCGAAGCTAGTGGAGGAGCTGCGCA
ACAGTCTGCCCGAGCTACCAGATGCAAGGGCTAAGAGGATAGCATCCCAGTATGGGGTGCGGATGGAGCTTGCAAAGATA
CTCGTCTCGCGTAAGGTTCTTGCAGACTTCTTTGAGGACGCTGCCAGGCTGTATAGGGGTAATTATGAGCGCATGGCCAA
CTACCTTGTAAATGACCTGCTGAACTGGCTAAAAGATGAGGACCTACGGGGACTCTACACCAAGGTCAAGCCTGAGCACC
TTGCAAAGCTCATGCAGCTCCTTGACCGCGGTGTTATCAGCATTAGACAAGCTAAGGAAATGGCTGAGCATATAGCTAAG
CAGGGTGCTGACCCCGAGAAGCTGGTTGAGAAGCTAGGGTTCCGTAGGATAGCGGATGTGGAGGTGCTGCGTAGCGTAGT
GGAGGAGGTGTTCAAGGAGTACCCCAAGGCCGTGAGGGATGCGCTGAAAAACCCGAAGGCGATAAACTTCCTGGTAGGCA
TGGTTATGAGGAAGACTCGGGGCAGGGCAGACCCAGCACTAGCCCGAAGACTTGTAGAGGAGAAGCTGAAGGAGGTAAAG
TCCTAG

Upstream 100 bases:

>100_bases
TGGGGGTGATATCTATTATTACTCCACAGCAGCCTCTGCAGTCACGATAACTGGTTTAACCTGCAGCAGATCTAGAACCC
TTCACGGTGCCAGACAGGGT

Downstream 100 bases:

>100_bases
GCTTCTAGCTTCACAGTCTCGACTTCAATACTCTTCGCGTACTTTTCGGCAGTAAACGCTAAACTCGTATCCTGCGATGA
TGGGTACAACCTCTATGCCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAVSGVKAVIGLEVHVQITSLKTKLFCSCSADYRGAPPNTHVCPVCLGLPGALPVVNEEAVKKAIQLCLAINGEVANILR
FDRKHYFYPDLPKNYQITQYLEPICRGGYIEIETGKGPKKIRIRRINLEEDPGRIVYPGGSPLASPYALVDYNRSGVALL
EIVTEPDMESPEEAVAFLEKLHSIVEHLGICDCRLEGSIRVDANVSIEGGERVEVKNIGGFSEVKKALYYEITRQRDLLM
KGRNVKRETRHWDPVRKVTLPARVKETEEDYRYMPDPNLPPVRISAKLVEELRNSLPELPDARAKRIASQYGVRMELAKI
LVSRKVLADFFEDAARLYRGNYERMANYLVNDLLNWLKDEDLRGLYTKVKPEHLAKLMQLLDRGVISIRQAKEMAEHIAK
QGADPEKLVEKLGFRRIADVEVLRSVVEEVFKEYPKAVRDALKNPKAINFLVGMVMRKTRGRADPALARRLVEEKLKEVK
S

Sequences:

>Translated_481_residues
MAVSGVKAVIGLEVHVQITSLKTKLFCSCSADYRGAPPNTHVCPVCLGLPGALPVVNEEAVKKAIQLCLAINGEVANILR
FDRKHYFYPDLPKNYQITQYLEPICRGGYIEIETGKGPKKIRIRRINLEEDPGRIVYPGGSPLASPYALVDYNRSGVALL
EIVTEPDMESPEEAVAFLEKLHSIVEHLGICDCRLEGSIRVDANVSIEGGERVEVKNIGGFSEVKKALYYEITRQRDLLM
KGRNVKRETRHWDPVRKVTLPARVKETEEDYRYMPDPNLPPVRISAKLVEELRNSLPELPDARAKRIASQYGVRMELAKI
LVSRKVLADFFEDAARLYRGNYERMANYLVNDLLNWLKDEDLRGLYTKVKPEHLAKLMQLLDRGVISIRQAKEMAEHIAK
QGADPEKLVEKLGFRRIADVEVLRSVVEEVFKEYPKAVRDALKNPKAINFLVGMVMRKTRGRADPALARRLVEEKLKEVK
S
>Mature_480_residues
AVSGVKAVIGLEVHVQITSLKTKLFCSCSADYRGAPPNTHVCPVCLGLPGALPVVNEEAVKKAIQLCLAINGEVANILRF
DRKHYFYPDLPKNYQITQYLEPICRGGYIEIETGKGPKKIRIRRINLEEDPGRIVYPGGSPLASPYALVDYNRSGVALLE
IVTEPDMESPEEAVAFLEKLHSIVEHLGICDCRLEGSIRVDANVSIEGGERVEVKNIGGFSEVKKALYYEITRQRDLLMK
GRNVKRETRHWDPVRKVTLPARVKETEEDYRYMPDPNLPPVRISAKLVEELRNSLPELPDARAKRIASQYGVRMELAKIL
VSRKVLADFFEDAARLYRGNYERMANYLVNDLLNWLKDEDLRGLYTKVKPEHLAKLMQLLDRGVISIRQAKEMAEHIAKQ
GADPEKLVEKLGFRRIADVEVLRSVVEEVFKEYPKAVRDALKNPKAINFLVGMVMRKTRGRADPALARRLVEEKLKEVKS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=36.0160965794769, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI115534094, Length=302, Percent_Identity=40.7284768211921, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=514, Percent_Identity=32.4902723735409, Blast_Score=229, Evalue=6e-61,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=33.3333333333333, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54479; Mature: 54348

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSGVKAVIGLEVHVQITSLKTKLFCSCSADYRGAPPNTHVCPVCLGLPGALPVVNEEA
CCCCCCCCEEEEEEEEEEEEECEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCHHH
VKKAIQLCLAINGEVANILRFDRKHYFYPDLPKNYQITQYLEPICRGGYIEIETGKGPKK
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCE
IRIRRINLEEDPGRIVYPGGSPLASPYALVDYNRSGVALLEIVTEPDMESPEEAVAFLEK
EEEEEECCCCCCCEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHH
LHSIVEHLGICDCRLEGSIRVDANVSIEGGERVEVKNIGGFSEVKKALYYEITRQRDLLM
HHHHHHHCCCCEEEECCCEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KGRNVKRETRHWDPVRKVTLPARVKETEEDYRYMPDPNLPPVRISAKLVEELRNSLPELP
CCCCCCHHHHCCCCHHHEECCHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DARAKRIASQYGVRMELAKILVSRKVLADFFEDAARLYRGNYERMANYLVNDLLNWLKDE
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
DLRGLYTKVKPEHLAKLMQLLDRGVISIRQAKEMAEHIAKQGADPEKLVEKLGFRRIADV
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
EVLRSVVEEVFKEYPKAVRDALKNPKAINFLVGMVMRKTRGRADPALARRLVEEKLKEVK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure 
AVSGVKAVIGLEVHVQITSLKTKLFCSCSADYRGAPPNTHVCPVCLGLPGALPVVNEEA
CCCCCCCEEEEEEEEEEEEECEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCHHH
VKKAIQLCLAINGEVANILRFDRKHYFYPDLPKNYQITQYLEPICRGGYIEIETGKGPKK
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCE
IRIRRINLEEDPGRIVYPGGSPLASPYALVDYNRSGVALLEIVTEPDMESPEEAVAFLEK
EEEEEECCCCCCCEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHH
LHSIVEHLGICDCRLEGSIRVDANVSIEGGERVEVKNIGGFSEVKKALYYEITRQRDLLM
HHHHHHHCCCCEEEECCCEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KGRNVKRETRHWDPVRKVTLPARVKETEEDYRYMPDPNLPPVRISAKLVEELRNSLPELP
CCCCCCHHHHCCCCHHHEECCHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DARAKRIASQYGVRMELAKILVSRKVLADFFEDAARLYRGNYERMANYLVNDLLNWLKDE
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
DLRGLYTKVKPEHLAKLMQLLDRGVISIRQAKEMAEHIAKQGADPEKLVEKLGFRRIADV
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
EVLRSVVEEVFKEYPKAVRDALKNPKAINFLVGMVMRKTRGRADPALARRLVEEKLKEVK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]