Click here to switch to the map view.

The map label for this gene is aph [H]

Identifier: 121639734

GI number: 121639734

Start: 4254050

End: 4254805

Strand: Direct

Name: aph [H]

Synonym: BCG_3879

Alternate gene names: 121639734

Gene position: 4254050-4254805 (Clockwise)

Preceding gene: 121639729

Following gene: 121639735

Centisome position: 97.25

GC content: 70.11

Gene sequence:

>756_bases
GTGTCCTTCCCCTCATCGCCACCCGCGCTGCCCGCGATCGTTGCCCGGTTTGCCGTCGGCAGGCCGGTGCGCGCGGTGTG
GGTCAACGAACTGGGCGGCGTCACCTTCCGGGTGGACTCCGGCATGGGCGCCGGCTGCGAGTTCATCAAGGTCGCCAGGA
GGGGTACCGCCGACTTCGCTAATGAGGCGCGGCGGCTGCGCTGGGCCGCGCCGTACCTGGCGGTGCCGCGGGTACTGGGT
GTCGGGGTCGACGGCGATTGGGCCTGGTTGCACACCGATGCGCTGCCCGGCTTGTCCGCGGTGCACCCGCGCTGGCGGGC
GTCCCCGCAGGTCGCGGTCCCGGCGCTGGGTGCGGGGCTGCGCACCCTGCACGACAGCTTGCCGGTGCACTCATGTCCGT
TCGACTGGTCGACGGCCAGCCGGCTGGCCAAGCTGGCCCCGGCGCGACGCGCGGAACTGGGTGACTCACCGCCGGTTGAT
CGGTTGGTCGTCTGTCACGGCGACGCGTGCTCACCCAACACCATCCTCGATGACACCGGCCGCTGTTGCGGACACGTCGA
CTTCGGCAATCTCGGTGTGGCCGATCGGTGGGCCGACCTCGCGGTCGCGACGCTGTCGTTGCAATGGAACTTTCCCGACT
ACCCGGGCCAGGTCAGAGATGACGAGTTCTTCGCCGCCTACGGTGTGGCGCCGGACCCGGCTCGCATCGACTACTACCGC
CGGCTGTGGCAGGCCGAAGACGACAGCTCACGCTAA

Upstream 100 bases:

>100_bases
TGACGGTGCCGTATACCGGTTCCACAGCAGTTCAGCCTAGTGGTCCCGGCTGCAAGCCGCCCAAAGTGGCGAAAACCCAA
ATTGACGAAAGAGGTGAGCC

Downstream 100 bases:

>100_bases
GCTCGAGGCTGCGCTTTGCGCTCGTAAGCTCTTCCGAAAGGTAGCTGTGCAGGTCACAAGCGTTGGTCACGCCGGCTTTC
TGATCCAGACCCAGGCCGGC

Product: putative phosphotransferase

Products: NA

Alternate protein names: APH(3')V; Kanamycin kinase, type V; Neomycin-kanamycin phosphotransferase type V [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFANEARRLRWAAPYLAVPRVLG
VGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVD
RLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR
RLWQAEDDSSR

Sequences:

>Translated_251_residues
MSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFANEARRLRWAAPYLAVPRVLG
VGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVD
RLVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR
RLWQAEDDSSR
>Mature_250_residues
SFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFANEARRLRWAAPYLAVPRVLGV
GVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGLRTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDR
LVVCHGDACSPNTILDDTGRCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYRR
LWQAEDDSSR

Specific function: Resistance to kanamycin and structurally-related aminoglycosides, including amikacin [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 27242; Mature: 27111

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFA
CCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHCCEEEEECCCCCCCHHHHHHHHCCCHHHH
NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL
HHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCEECHHHHHHH
RTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG
HHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEECCCCCCCCEECCCC
RCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR
CEECCCCCCCCCCHHHHHHEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
RLWQAEDDSSR
HHHCCCCCCCC
>Mature Secondary Structure 
SFPSSPPALPAIVARFAVGRPVRAVWVNELGGVTFRVDSGMGAGCEFIKVARRGTADFA
CCCCCCCHHHHHHHHHHCCCCCEEEEEHHHCCEEEEECCCCCCCHHHHHHHHCCCHHHH
NEARRLRWAAPYLAVPRVLGVGVDGDWAWLHTDALPGLSAVHPRWRASPQVAVPALGAGL
HHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCEECHHHHHHH
RTLHDSLPVHSCPFDWSTASRLAKLAPARRAELGDSPPVDRLVVCHGDACSPNTILDDTG
HHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEECCCCCCCCEECCCC
RCCGHVDFGNLGVADRWADLAVATLSLQWNFPDYPGQVRDDEFFAAYGVAPDPARIDYYR
CEECCCCCCCCCCHHHHHHEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
RLWQAEDDSSR
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6310563; 2987648 [H]