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The map label for this gene is fbiB

Identifier: 121639151

GI number: 121639151

Start: 3594018

End: 3595364

Strand: Direct

Name: fbiB

Synonym: BCG_3291

Alternate gene names: 121639151

Gene position: 3594018-3595364 (Clockwise)

Preceding gene: 121639150

Following gene: 121639152

Centisome position: 82.16

GC content: 68.67

Gene sequence:

>1347_bases
TTGACCGGCCCCGAACATGGCTCCGCCTCGACCATCGAGATCCTGCCCGTCATCGGGCTGCCCGAATTCCGTCCCGGCGA
CGATCTGAGCGCCGCCGTCGCCGCGGCGGCACCGTGGCTACGCGACGGTGACGTCGTGGTGGTTACCAGCAAGGTGGTGT
CCAAATGCGAGGGCCGGCTGGTTCCGGCTCCCGAAGACCCCGAGCAAAGAGACCGATTGCGCCGCAAGCTGATCGAGGAT
GAGGCAGTGCGCGTGTTGGCGCGCAAGGACCGCACGTTGATCACCGAGAATCGACTCGGGCTGGTTCAGGCGGCCGCCGG
CGTGGACGGATCCAACGTCGGCCGGTCCGAGTTAGCGCTGCTGCCGGTCGATCCTGACGCCAGTGCCGCAACCTTGCGCG
CCGGGCTGCGCGAGCGGCTCGGCGTCACCGTCGCCGTGGTCATCACCGACACCATGGGACGCGCCTGGCGCAACGGCCAG
ACCGATGCCGCAGTCGGCGCTGCCGGTCTGGCGGTGCTGCGCAACTATGCCGGTGTCCGCGACCCATACGGCAATGAGTT
GGTGGTCACCGAGGTCGCAGTCGCCGACGAGATCGCCGCGGCCGCCGACTTGGTCAAAGGCAAACTGACCGCGACGCCGG
TGGCGGTGGTGCGTGGGTTCGGCGTGTCCGACGACGGCTCGACAGCCCGGCAACTGCTGCGGCCGGGCGCCAACGACCTG
TTCTGGCTCGGGACCGCCGAAGCGCTCGAGCTGGGTCGCCAGCAAGCCCAACTGTTGCGCAGGTCCGTTCGCCGGTTTAG
CACCGATCCGGTGCCGGGCGACCTCGTCGAGGCTGCGGTCGCCGAGGCCCTCACCGCGCCAGCCCCACATCACACCCGGC
CGACCCGATTCGTGTGGCTGCAGACACCGGCCATCCGCGCGCGGCTGCTAGATCGGATGAAAGACAAGTGGCGGTCTGAT
CTCACCAGTGACGGCTTGCCCGCCGACGCGATAGAACGCCGGGTGGCACGCGGCCAGATCCTCTATGACGCACCCGAAGT
CGTCATACCGATGCTGGTGCCCGACGGAGCACACAGCTACCCCGATGCCGCCCGCACCGACGCCGAGCACACCATGTTCA
CGGTCGCCGTCGGAGCGGCCGTACAAGCCTTGCTGGTCGCGCTGGCCGTGCGCGGGCTGGGCAGTTGCTGGATCGGCTCG
ACGATCTTTGCCGCTGACCTGGTCCGCGACGAGCTGGACCTGCCAGTCGACTGGGAGCCGTTGGGCGCCATCGCGATCGG
ATATGCCGACGAGCCGTCCGGGTTGCGCGACCCGGTGCCTGCCGCCGATTTGCTGATCCTGAAGTGA

Upstream 100 bases:

>100_bases
TTGACGGGGTGACGGTGCGGTCGGTGCCGCTGCTGATGACCGACCCGAACGCGACGGCTGAGATGGTTCGCGCCGGGTGC
GACCTTGCGGGAGTGGTAGC

Downstream 100 bases:

>100_bases
CATTCGCTCTAGCGACGATAGGCTACCCAGACATGGCGGTCCTGCAGCCGATGCCAACCATCAACCTCCCGACGGATCAA
TTCACCGCGTTCGGTCAAAA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDPEQRDRLRRKLIED
EAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELALLPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQ
TDAAVGAAGLAVLRNYAGVRDPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDL
FWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWLQTPAIRARLLDRMKDKWRSD
LTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGS
TIFAADLVRDELDLPVDWEPLGAIAIGYADEPSGLRDPVPAADLLILK

Sequences:

>Translated_448_residues
MTGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDPEQRDRLRRKLIED
EAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELALLPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQ
TDAAVGAAGLAVLRNYAGVRDPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDL
FWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWLQTPAIRARLLDRMKDKWRSD
LTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGS
TIFAADLVRDELDLPVDWEPLGAIAIGYADEPSGLRDPVPAADLLILK
>Mature_447_residues
TGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDPEQRDRLRRKLIEDE
AVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELALLPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQT
DAAVGAAGLAVLRNYAGVRDPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDLF
WLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWLQTPAIRARLLDRMKDKWRSDL
TSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGST
IFAADLVRDELDLPVDWEPLGAIAIGYADEPSGLRDPVPAADLLILK

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives (Probable). M.bovis F420 contains primarily

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFE_MYCBO (Q7TWV3)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_856935.1
- ProteinModelPortal:   Q7TWV3
- SMR:   Q7TWV3
- EnsemblBacteria:   EBMYCT00000015419
- GeneID:   1093973
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3290
- GeneTree:   EBGT00050000015749
- HOGENOM:   HBG578188
- OMA:   PAPHHTT
- ProtClustDB:   PRK13294
- BioCyc:   MBOV233413:MB3290-MONOMER
- HAMAP:   MF_01259
- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415
- TIGRFAMs:   TIGR03553
- TIGRFAMs:   TIGR01916

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: NA

Molecular weight: Translated: 47579; Mature: 47448

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRL
CCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCC
VPAPEDPEQRDRLRRKLIEDEAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCCCCCEEE
LPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQTDAAVGAAGLAVLRNYAGVR
EECCCCCCHHHHHHHHHHHHCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
DPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDL
CCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCE
FWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWL
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
QTPAIRARLLDRMKDKWRSDLTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSY
ECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCC
PDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGSTIFAADLVRDELDLPVDWEP
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LGAIAIGYADEPSGLRDPVPAADLLILK
CCEEEEECCCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
TGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRL
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCC
VPAPEDPEQRDRLRRKLIEDEAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCCCCCEEE
LPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQTDAAVGAAGLAVLRNYAGVR
EECCCCCCHHHHHHHHHHHHCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
DPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDL
CCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCE
FWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWL
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
QTPAIRARLLDRMKDKWRSDLTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSY
ECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCC
PDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGSTIFAADLVRDELDLPVDWEP
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LGAIAIGYADEPSGLRDPVPAADLLILK
CCEEEEECCCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972