Click here to switch to the map view.

The map label for this gene is eco57IBM [H]

Identifier: 121639152

GI number: 121639152

Start: 3595660

End: 3597321

Strand: Direct

Name: eco57IBM [H]

Synonym: BCG_3292

Alternate gene names: 121639152

Gene position: 3595660-3597321 (Clockwise)

Preceding gene: 121639151

Following gene: 121639156

Centisome position: 82.2

GC content: 63.24

Gene sequence:

>1662_bases
ATGCAACCTAGTCATCCGACGCGCCCTGGTGCGGTCATCAGATATGTCGGTAGCTCCCTTGATACTTGTCCCATGACGAC
GTTCGCCGGCAAAACGGCTGCGTCCGCTGACAAGGTGCGCGGGGGCTACTACACGCCGCCGGCGGTGGCCCGATTCCTTG
CCCACTGGGTTCACCAGGCGGGGCCGAAGATCCTCGAACCATCCTGCGGCGATGGCCGAATCCTGCGCGAACTCTCCGCC
ATCACAGACCACGCGCACGGTGTGGAACTCGTTGCGCGCGAGGCGAAAAAGTCGCGGGACTTCGCGTCCGTCGACACTGA
GAACCTTTTTACCTGGCTGCACAAGACCCAACTCGGCAGCTGGGATGGCGTTGCCGGCAACCCGCCCTACATCCGCTTCG
GAAACTGGGCATCCGAACAACGGGATCCGGCACTCGAATTGATGCGGCGTGTGGGCCTACGACCGACCAAACTGACCAAT
GCCTGGGTCCCGTTTGTCGTGGCGAGCACGACGCTAGCGCGTGACGGCGGCCGAGTGGGCCTGGTGGTCCCGGCGGAATT
GCTTCAAGTCACCTACGCGGCGCAGCTACGCGAATTCCTGCTGAGCCGCTATCGGGAGATCACCCTGGTTACCTTCGAGC
GGCTGGTGTTCGACGGAATCCTGCAGGAAGTTGTGCTGTTCTGCGGCGTCGTCGGTCCCGGTCCTGCACACATACGCACC
GTCAGGCTCGGCGATGCGAACGATCTGAACGCGCTGGGGGACAAGGACTTCACCAATGAGTCAGCGCCGGCGCTTCTCCA
CGAAAAGGAGAAGTGGACCAAGTACTTCCTCGACCCCGCTCAAATCCGGCTACTGCGAGGACTCAAACAGTCCGCCACTA
TGATCAGGCTCGGCGAACTGGCCGACGTGGATGTGGGCATCGTGACCGGCCGCAACAGCTTCTTCACGTTCACCGATGCC
AAGGCACAAGCGCTGGGATTGCGAGCGCACTGCGTTCCCCTGGTCTCTCGCAGCGCCCAACTCAGCGGGCTGATCTATGA
CGAGGATTGCCGGGCATGCGATGTCGCCGGCAACCACCGAACGTGGCTACTCGACGCCGCGGACTATCCAACCGATCCAG
CTCTCGTCGCTCACATCACCGCGGGTGAAGCGGCCGGCGTCCACCTCGGCTACAAGTGCTCGATCCGCAAGCCATGGTGG
AGCACACCATCGCTGTGGATGCCCGACCTCTTTATGCTGCGCCAGATCCACTTCGCCCCGCGGCTGACCGTCAACGCTGC
CGCGGCGACCAGCACCGATACCGTGCACCGGGTCCGGCTCGACCCGAACGTCGATCCGGCAACTCTTGCCGCGGTGTTCC
ACAACAGCGCGACATTCGCGTTCGCCGAGATCATGGGCCGCAGTTATGGGGGCGGCATCTTGGAGTTGGAGCCTAGGGAA
GCCGAGCAACTACCTATGCCACCGCCGGCGTACGGGAGCGCAGAACTTGCCCAGGATGTTGATCTCCTGCTGAAAGCAAA
CGAGATCGACAAGGCGCTCGACGTCGTGGACCGTCACGTTCTGATCGACGGGCTCGGCTTGTCGCCGCGCCTGGTCGCAG
GTTGCCGAGCGGCATGGCTCACGCTCCGCGACCGCAGGACCAAGCGCGGATCTCGGCGATAA

Upstream 100 bases:

>100_bases
CAACATCCCATTGGTGGTTCCGAACGCCAACCAGCTGTAACCTTCCCAAGCGCCGACGTGTACCGCTGCTATCCGGCCCG
ATTCCAGGGACAGCCACCCC

Downstream 100 bases:

>100_bases
CCGCGGCGGGTGAGCGCCTCGCGTGCCCGGCCAACGATGTCGATCTCGGCGCAAGAAGCTCAAACGTCGGACGAGTAACG
GATCCCGCCGTCGGGAAGAA

Product: putative DNA methylase

Products: NA

Alternate protein names: M.Eco57IB; Adenine-specific methyltransferase Eco57IB [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSA
ITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTN
AWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRT
VRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDA
KAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPRE
AEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR

Sequences:

>Translated_553_residues
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSA
ITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTN
AWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRT
VRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDA
KAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPRE
AEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR
>Mature_553_residues
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQAGPKILEPSCGDGRILRELSA
ITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGSWDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTN
AWVPFVVASTTLARDGGRVGLVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRT
VRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGELADVDVGIVTGRNSFFTFTDA
KAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHRTWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWW
STPSLWMPDLFMLRQIHFAPRLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPRE
AEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWLTLRDRRTKRGSRR

Specific function: This methylase recognizes the double-stranded sequence 5'-CTGAAG-3' in one strand and 5'-CTTCAG-3' in the other, causes specific methylation on A-5 on one strand, and protects the DNA from cleavage by the Eco57I endonuclease [H]

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 60674; Mature: 60674

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQA
CCCCCCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHC
GPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGS
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
WDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVG
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
LVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRT
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCC
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHR
CCCCEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHCCHHHCCEEECCCCCEECCCCCCC
TWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAP
EEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCC
RLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPRE
CEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCC
AEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
TLRDRRTKRGSRR
HHHHHHCCCCCCC
>Mature Secondary Structure
MQPSHPTRPGAVIRYVGSSLDTCPMTTFAGKTAASADKVRGGYYTPPAVARFLAHWVHQA
CCCCCCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHC
GPKILEPSCGDGRILRELSAITDHAHGVELVAREAKKSRDFASVDTENLFTWLHKTQLGS
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
WDGVAGNPPYIRFGNWASEQRDPALELMRRVGLRPTKLTNAWVPFVVASTTLARDGGRVG
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
LVVPAELLQVTYAAQLREFLLSRYREITLVTFERLVFDGILQEVVLFCGVVGPGPAHIRT
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
VRLGDANDLNALGDKDFTNESAPALLHEKEKWTKYFLDPAQIRLLRGLKQSATMIRLGEL
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCC
ADVDVGIVTGRNSFFTFTDAKAQALGLRAHCVPLVSRSAQLSGLIYDEDCRACDVAGNHR
CCCCEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHCCHHHCCEEECCCCCEECCCCCCC
TWLLDAADYPTDPALVAHITAGEAAGVHLGYKCSIRKPWWSTPSLWMPDLFMLRQIHFAP
EEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCC
RLTVNAAAATSTDTVHRVRLDPNVDPATLAAVFHNSATFAFAEIMGRSYGGGILELEPRE
CEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCC
AEQLPMPPPAYGSAELAQDVDLLLKANEIDKALDVVDRHVLIDGLGLSPRLVAGCRAAWL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHH
TLRDRRTKRGSRR
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1334261; 1334260 [H]