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The map label for this gene is lipB

Identifier: 121638098

GI number: 121638098

Start: 2463228

End: 2463920

Strand: Direct

Name: lipB

Synonym: BCG_2233

Alternate gene names: 121638098

Gene position: 2463228-2463920 (Clockwise)

Preceding gene: 121638097

Following gene: 121638099

Centisome position: 56.31

GC content: 68.83

Gene sequence:

>693_bases
GTGACGGGTTCTATCCGGTCGAAGCTGTCCGCGATCGACGTCCGCCAGCTGGGGACCGTCGACTACCGGACCGCGTGGCA
GCTACAGCGAGAGCTAGCCGACGCCCGGGTCGCCGGCGGCGCCGACACGCTGCTGCTGTTGGAACACCCCGCGGTCTACA
CCGCCGGACGGCGTACCGAGACACACGAGCGACCCATTGACGGCACTCCGGTCGTCGGCACCGACCGCGGCGGCAAGATC
ACCTGGCACGGTCCGGGGCAATTGGTCGGCTACCCGATCATCGGGCTGGCCGAACCCCTCGACGTGGTCAATTACGTTCG
GCGCCTTGAAGAATCGCTGATCCAAGTCTGCGCCGATCTGGGCCTGCACGCCGGCCGCGTCGACGGCCGGTCCGGGGTCT
GGCTGCCCGGCAGGCCGGCGCGCAAGGTCGCGGCCATCGGTGTCCGGGTGTCGCGGGCGACGACACTGCACGGGTTTGCG
CTCAACTGCGATTGTGATTTGGCTGCCTTCACCGCCATCGTGCCATGCGGAATCAGTGACGCCGCAGTGACATCGCTGTC
CGCCGAACTCGGCCGTACGGTCACCGTCGACGAGGTCCGCGCGACGGTCGCCGCCGCTGTCTGCGCCGCTCTGGACGGCG
TCCTACCGGTCGGTGACCGCGTGCCCTCACACGCCGTACCATCGCCGTTATGA

Upstream 100 bases:

>100_bases
AACACCATTGGCGAGGCGCTCGGCTACGCCACCACCCGGCCCGGCTAGGCTTGACCCCGTCTGCCCAGCCGTGCGCTGGC
GGCCGAGTAGCCTAGCTATC

Downstream 100 bases:

>100_bases
GTGTCGCTGCCGAGGGCCGGCGCCTGTTACGCCTGGAGGTGCGCAACGCGCAGACCCCAATCGAGCGCAAACCGCCGTGG
ATCAAGACACGAGCCCGCAT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTETHERPIDGTPVVGTDRGGKI
TWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADLGLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFA
LNCDCDLAAFTAIVPCGISDAAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL

Sequences:

>Translated_230_residues
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTETHERPIDGTPVVGTDRGGKI
TWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADLGLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFA
LNCDCDLAAFTAIVPCGISDAAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
>Mature_229_residues
TGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTETHERPIDGTPVVGTDRGGKIT
WHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADLGLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFAL
NCDCDLAAFTAIVPCGISDAAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=207, Percent_Identity=41.0628019323672, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI87081767, Length=178, Percent_Identity=34.8314606741573, Blast_Score=105, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=218, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21355483, Length=224, Percent_Identity=35.2678571428571, Blast_Score=123, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_MYCBO (Q7VEN4)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855889.1
- ProteinModelPortal:   Q7VEN4
- SMR:   Q7VEN4
- EnsemblBacteria:   EBMYCT00000018030
- GeneID:   1091365
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2240
- GeneTree:   EBGT00050000016282
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14345
- BioCyc:   MBOV233413:MB2240-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24153; Mature: 24022

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTE
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
THERPIDGTPVVGTDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADL
CCCCCCCCCCEEECCCCCEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFALNCDCDLAAFTAIVPCGISD
CCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECEEEEEEEEEECCCCHHHHHHHHCCCCCH
AAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
HHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGSIRSKLSAIDVRQLGTVDYRTAWQLQRELADARVAGGADTLLLLEHPAVYTAGRRTE
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
THERPIDGTPVVGTDRGGKITWHGPGQLVGYPIIGLAEPLDVVNYVRRLEESLIQVCADL
CCCCCCCCCCEEECCCCCEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GLHAGRVDGRSGVWLPGRPARKVAAIGVRVSRATTLHGFALNCDCDLAAFTAIVPCGISD
CCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECEEEEEEEEEECCCCHHHHHHHHCCCCCH
AAVTSLSAELGRTVTVDEVRATVAAAVCAALDGVLPVGDRVPSHAVPSPL
HHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972