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The map label for this gene is lipA

Identifier: 121638099

GI number: 121638099

Start: 2463917

End: 2464852

Strand: Direct

Name: lipA

Synonym: BCG_2234

Alternate gene names: 121638099

Gene position: 2463917-2464852 (Clockwise)

Preceding gene: 121638098

Following gene: 121638100

Centisome position: 56.32

GC content: 67.52

Gene sequence:

>936_bases
ATGAGTGTCGCTGCCGAGGGCCGGCGCCTGTTACGCCTGGAGGTGCGCAACGCGCAGACCCCAATCGAGCGCAAACCGCC
GTGGATCAAGACACGAGCCCGCATCGGGCCGGAGTACACCGAGCTGAAGAACCTGGTCCGCCGCGAGGGGCTGCACACGG
TCTGCGAGGAGGCCGGCTGCCCCAACATCTTCGAATGCTGGGAGGACCGAGAAGCCACCTTCCTGATCGGCGGTGACCAG
TGCACCCGCCGATGCGATTTCTGCCAGATCGACACCGGAAAGCCCGCCGAGCTGGACCGCGACGAGCCACGCCGAGTCGC
CGACAGCGTGCGCACGATGGGCCTGCGCTATGCCACCGTCACCGGCGTGGCTCGCGACGACCTGCCTGACGGCGGGGCCT
GGCTGTACGCCGCGACCGTGCGCGCCATCAAGGAACTCAATCCGTCGACCGGCGTCGAACTGCTGATTCCCGACTTCAAC
GGCGAACCAACCCGGCTGGCCGAGGTCTTCGAGTCCGGCCCGGAAGTCCTGGCACACAATGTCGAAACCGTGCCCCGTAT
CTTCAAGCGGATCCGGCCGGCGTTCACGTACCGGCGCAGCCTGGGTGTGCTTACCGCTGCGCGCGACGCCGGCCTGGTCA
CCAAGAGCAACCTCATCCTCGGCCTGGGCGAAACCTCCGACGAGGTGCGCACCGCCCTGGGCGATCTGCGCGACGCCGGC
TGCGACATCGTTACCATCACCCAATACCTGCGGCCGTCGGCGCGCCACCATCCGGTCGAGCGCTGGGTGAAGCCCGAGGA
GTTCGTCCAGTTCGCGCGATTCGCCGAAGGGCTGGGCTTCGCCGGGGTATTGGCGGGACCCCTGGTTAGGTCGTCATATC
GGGCGGGCCGGCTCTACGAACAGGCACGTAACTCACGGGCCTTGGCATCCCGCTAG

Upstream 100 bases:

>100_bases
ACGAGGTCCGCGCGACGGTCGCCGCCGCTGTCTGCGCCGCTCTGGACGGCGTCCTACCGGTCGGTGACCGCGTGCCCTCA
CACGCCGTACCATCGCCGTT

Downstream 100 bases:

>100_bases
CCAGCGTTTACGTATTCTGGACGATTATGGCGAAACCCCGAAATGCCGCTGAAAGCAAGGCCGCCAAAGCTCAGGCAAAC
GCTGCTCGTAAGGCTGCCGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSVAAEGRRLLRLEVRNAQTPIERKPPWIKTRARIGPEYTELKNLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQ
CTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAATVRAIKELNPSTGVELLIPDFN
GEPTRLAEVFESGPEVLAHNVETVPRIFKRIRPAFTYRRSLGVLTAARDAGLVTKSNLILGLGETSDEVRTALGDLRDAG
CDIVTITQYLRPSARHHPVERWVKPEEFVQFARFAEGLGFAGVLAGPLVRSSYRAGRLYEQARNSRALASR

Sequences:

>Translated_311_residues
MSVAAEGRRLLRLEVRNAQTPIERKPPWIKTRARIGPEYTELKNLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQ
CTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAATVRAIKELNPSTGVELLIPDFN
GEPTRLAEVFESGPEVLAHNVETVPRIFKRIRPAFTYRRSLGVLTAARDAGLVTKSNLILGLGETSDEVRTALGDLRDAG
CDIVTITQYLRPSARHHPVERWVKPEEFVQFARFAEGLGFAGVLAGPLVRSSYRAGRLYEQARNSRALASR
>Mature_310_residues
SVAAEGRRLLRLEVRNAQTPIERKPPWIKTRARIGPEYTELKNLVRREGLHTVCEEAGCPNIFECWEDREATFLIGGDQC
TRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATVTGVARDDLPDGGAWLYAATVRAIKELNPSTGVELLIPDFNG
EPTRLAEVFESGPEVLAHNVETVPRIFKRIRPAFTYRRSLGVLTAARDAGLVTKSNLILGLGETSDEVRTALGDLRDAGC
DIVTITQYLRPSARHHPVERWVKPEEFVQFARFAEGLGFAGVLAGPLVRSSYRAGRLYEQARNSRALASR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=44.2105263157895, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=44.3089430894309, Blast_Score=203, Evalue=1e-52,
Organism=Escherichia coli, GI1786846, Length=297, Percent_Identity=41.4141414141414, Blast_Score=241, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI32564533, Length=291, Percent_Identity=42.6116838487972, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=43.7062937062937, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=45.8041958041958, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_MYCBO (P65284)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855890.1
- ProteinModelPortal:   P65284
- EnsemblBacteria:   EBMYCT00000015304
- GeneID:   1091366
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2241
- GeneTree:   EBGT00050000017436
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   MBOV233413:MB2241-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34719; Mature: 34588

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAAEGRRLLRLEVRNAQTPIERKPPWIKTRARIGPEYTELKNLVRREGLHTVCEEAGC
CCCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATV
CCHHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHE
TGVARDDLPDGGAWLYAATVRAIKELNPSTGVELLIPDFNGEPTRLAEVFESGPEVLAHN
ECCHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHC
VETVPRIFKRIRPAFTYRRSLGVLTAARDAGLVTKSNLILGLGETSDEVRTALGDLRDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCHHHHHHHHHHHHHCC
CDIVTITQYLRPSARHHPVERWVKPEEFVQFARFAEGLGFAGVLAGPLVRSSYRAGRLYE
CCCEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QARNSRALASR
HHHCCCCCCCC
>Mature Secondary Structure 
SVAAEGRRLLRLEVRNAQTPIERKPPWIKTRARIGPEYTELKNLVRREGLHTVCEEAGC
CCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PNIFECWEDREATFLIGGDQCTRRCDFCQIDTGKPAELDRDEPRRVADSVRTMGLRYATV
CCHHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHE
TGVARDDLPDGGAWLYAATVRAIKELNPSTGVELLIPDFNGEPTRLAEVFESGPEVLAHN
ECCHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHC
VETVPRIFKRIRPAFTYRRSLGVLTAARDAGLVTKSNLILGLGETSDEVRTALGDLRDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCHHHHHHHHHHHHHCC
CDIVTITQYLRPSARHHPVERWVKPEEFVQFARFAEGLGFAGVLAGPLVRSSYRAGRLYE
CCCEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QARNSRALASR
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972