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The map label for this gene is trpA

Identifier: 121637520

GI number: 121637520

Start: 1817606

End: 1818418

Strand: Direct

Name: trpA

Synonym: BCG_1651

Alternate gene names: 121637520

Gene position: 1817606-1818418 (Clockwise)

Preceding gene: 121637519

Following gene: 121637521

Centisome position: 41.55

GC content: 65.81

Gene sequence:

>813_bases
ATGGTGGCGGTGGAACAGAGCGAAGCAAGTAGGCTCGGGCCGGTTTTCGATTCCTGCCGTGCAAACAACCGCGCGGCATT
GATTGGTTACTTGCCGACCGGGTACCCGGACGTGCCAGCGTCGGTGGCCGCGATGACAGCGCTAGTTGAATCCGGTTGCG
ACATTATCGAAGTCGGGGTTCCGTATTCGGACCCGGGCATGGACGGCCCCACCATCGCCAGGGCAACCGAGGCGGCGCTC
CGTGGCGGGGTGCGAGTCCGGGATACGTTAGCCGCGGTCGAGGCCATCAGTATCGCCGGCGGGCGTGCGGTAGTGATGAC
CTACTGGAATCCGGTGCTGCGCTATGGGGTTGATGCATTCGCGCGGGATCTGGCGGCGGCCGGAGGACTCGGCCTGATCA
CTCCTGACCTCATTCCCGACGAGGCGCAACAGTGGCTGGCGGCATCCGAAGAGCATCGGTTGGATCGCATTTTCTTGGTC
GCGCCGTCCTCGACACCGGAGCGGTTGGCGGCCACCGTCGAGGCTTCACGCGGGTTCGTCTACGCGGCGTCGACGATGGG
GGTGACCGGGGCGCGGGATGCGGTGTCGCAGGCGGCACCCGAACTGGTGGGCCGGGTGAAGGCGGTGTCTGACATACCGG
TGGGCGTCGGTCTGGGTGTGCGGTCGCGCGCTCAAGCCGCGCAGATCGCCCAATACGCCGACGGTGTCATCGTTGGTTCC
GCATTGGTGACGGCGCTAACCGAGGGGTTGCCTAGATTGCGGGCACTGACCGGAGAGCTCGCTGCCGGGGTACGACTAGG
GATGTCCGCATGA

Upstream 100 bases:

>100_bases
AGTTGGGAAGGGGCGCGGTGATTGTGGTGAACCTGTCGGGACGTGGCGACAAAGATGTCGAGACGGCCGCGAAATGGTTT
GGCTTGCTGGGCAACGACTG

Downstream 100 bases:

>100_bases
TGCGGATGTTGCCCAGCTATATCCCCAGCCCACCGCGCGGGGTTTGGTACCTGGGCCCGCTACCCGTCCGCGCCTACGCA
GTTTGCGTTATCACCGGCAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MVAVEQSEASRLGPVFDSCRANNRAALIGYLPTGYPDVPASVAAMTALVESGCDIIEVGVPYSDPGMDGPTIARATEAAL
RGGVRVRDTLAAVEAISIAGGRAVVMTYWNPVLRYGVDAFARDLAAAGGLGLITPDLIPDEAQQWLAASEEHRLDRIFLV
APSSTPERLAATVEASRGFVYAASTMGVTGARDAVSQAAPELVGRVKAVSDIPVGVGLGVRSRAQAAQIAQYADGVIVGS
ALVTALTEGLPRLRALTGELAAGVRLGMSA

Sequences:

>Translated_270_residues
MVAVEQSEASRLGPVFDSCRANNRAALIGYLPTGYPDVPASVAAMTALVESGCDIIEVGVPYSDPGMDGPTIARATEAAL
RGGVRVRDTLAAVEAISIAGGRAVVMTYWNPVLRYGVDAFARDLAAAGGLGLITPDLIPDEAQQWLAASEEHRLDRIFLV
APSSTPERLAATVEASRGFVYAASTMGVTGARDAVSQAAPELVGRVKAVSDIPVGVGLGVRSRAQAAQIAQYADGVIVGS
ALVTALTEGLPRLRALTGELAAGVRLGMSA
>Mature_270_residues
MVAVEQSEASRLGPVFDSCRANNRAALIGYLPTGYPDVPASVAAMTALVESGCDIIEVGVPYSDPGMDGPTIARATEAAL
RGGVRVRDTLAAVEAISIAGGRAVVMTYWNPVLRYGVDAFARDLAAAGGLGLITPDLIPDEAQQWLAASEEHRLDRIFLV
APSSTPERLAATVEASRGFVYAASTMGVTGARDAVSQAAPELVGRVKAVSDIPVGVGLGVRSRAQAAQIAQYADGVIVGS
ALVTALTEGLPRLRALTGELAAGVRLGMSA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=221, Percent_Identity=27.6018099547511, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=38.9121338912134, Blast_Score=165, Evalue=9e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_MYCBO (P66981)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855292.1
- ProteinModelPortal:   P66981
- SMR:   P66981
- EnsemblBacteria:   EBMYCT00000016959
- GeneID:   1092573
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1639
- GeneTree:   EBGT00050000016358
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   MBOV233413:MB1639-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27728; Mature: 27728

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 57-57 ACT_SITE 68-68

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVEQSEASRLGPVFDSCRANNRAALIGYLPTGYPDVPASVAAMTALVESGCDIIEVGV
CCCCCCCHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECC
PYSDPGMDGPTIARATEAALRGGVRVRDTLAAVEAISIAGGRAVVMTYWNPVLRYGVDAF
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
ARDLAAAGGLGLITPDLIPDEAQQWLAASEEHRLDRIFLVAPSSTPERLAATVEASRGFV
HHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEE
YAASTMGVTGARDAVSQAAPELVGRVKAVSDIPVGVGLGVRSRAQAAQIAQYADGVIVGS
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ALVTALTEGLPRLRALTGELAAGVRLGMSA
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVAVEQSEASRLGPVFDSCRANNRAALIGYLPTGYPDVPASVAAMTALVESGCDIIEVGV
CCCCCCCHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECC
PYSDPGMDGPTIARATEAALRGGVRVRDTLAAVEAISIAGGRAVVMTYWNPVLRYGVDAF
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
ARDLAAAGGLGLITPDLIPDEAQQWLAASEEHRLDRIFLVAPSSTPERLAATVEASRGFV
HHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEE
YAASTMGVTGARDAVSQAAPELVGRVKAVSDIPVGVGLGVRSRAQAAQIAQYADGVIVGS
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ALVTALTEGLPRLRALTGELAAGVRLGMSA
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972